##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3643208
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.403992305682245	33.0	31.0	34.0	29.0	34.0
2	31.524460310803008	34.0	31.0	34.0	28.0	34.0
3	31.599154371641696	34.0	31.0	34.0	28.0	34.0
4	35.08827988959181	37.0	35.0	37.0	32.0	37.0
5	34.83303149312364	37.0	35.0	37.0	32.0	37.0
6	34.78868925408596	37.0	35.0	37.0	32.0	37.0
7	34.65901754717271	37.0	35.0	37.0	32.0	37.0
8	34.621730079644095	37.0	35.0	37.0	32.0	37.0
9	36.235995584111585	39.0	37.0	39.0	32.0	39.0
10-11	36.08521926280355	39.0	37.0	39.0	32.0	39.0
12-13	36.01149728481053	39.0	37.0	39.0	31.0	39.0
14-15	37.24222018067593	40.0	38.0	41.0	31.0	41.0
16-17	37.21779267063533	40.0	38.0	41.0	31.0	41.0
18-19	37.18322437258592	40.0	38.0	41.0	31.0	41.0
20-21	37.14731728191199	40.0	38.0	41.0	31.0	41.0
22-23	37.04127433844019	40.0	38.0	41.0	31.0	41.0
24-25	36.96497990232784	40.0	37.0	41.0	31.0	41.0
26-27	36.824117783008816	40.0	37.0	41.0	30.0	41.0
28-29	36.67375551986052	40.0	37.0	41.0	30.0	41.0
30-31	36.52892121449008	40.0	37.0	41.0	30.0	41.0
32-33	36.476043640659555	40.0	36.5	41.0	30.0	41.0
34-35	36.60396153609676	40.0	37.0	41.0	30.0	41.0
36-37	36.57707273370063	40.0	37.0	41.0	30.0	41.0
38-39	36.48791065456598	40.0	37.0	41.0	30.0	41.0
40-41	36.36386571944287	40.0	36.5	41.0	29.5	41.0
42-43	36.259443188530554	40.0	36.0	41.0	29.0	41.0
44-45	36.122628600947294	40.0	36.0	41.0	28.5	41.0
46-47	35.96241128697565	40.0	36.0	41.0	28.0	41.0
48-49	35.8012365750185	40.0	35.0	41.0	27.5	41.0
50-51	35.647558278308566	40.0	35.0	41.0	27.0	41.0
52-53	35.47337250576964	39.0	35.0	41.0	27.0	41.0
54-55	35.26461720000614	39.0	35.0	41.0	26.0	41.0
56-57	35.00620675514547	39.0	35.0	41.0	26.0	41.0
58-59	34.78148735949196	38.5	35.0	40.5	26.0	41.0
60-61	34.50102601882736	38.0	34.5	40.0	25.5	41.0
62-63	34.1973402287215	37.5	34.0	40.0	25.0	41.0
64-65	33.88701784251681	37.0	34.0	40.0	24.0	41.0
66-67	33.555377293857504	36.0	34.0	39.0	23.0	41.0
68-69	33.19409871739412	36.0	34.0	39.0	22.5	41.0
70-71	32.81580354456841	35.0	33.0	38.5	22.0	40.0
72-73	32.42234069534322	35.0	33.0	37.5	20.5	40.0
74-75	32.01567176510372	35.0	33.0	37.0	20.0	39.0
76-77	29.277696469704722	33.0	29.0	34.5	13.0	36.5
78-79	30.93535148144163	34.5	31.5	36.0	16.5	37.0
80-81	31.13576715905323	35.0	32.0	35.5	18.0	37.0
82-83	30.97510820134343	35.0	32.0	35.0	17.5	37.0
84-85	30.75040019674968	35.0	32.0	35.0	13.5	36.0
86-87	30.511776297153496	35.0	32.0	35.0	8.5	36.0
88-89	30.242043825112376	35.0	32.0	35.0	2.0	36.0
90-91	30.061785245311274	34.5	31.5	35.0	2.0	35.0
92-93	29.915149093875506	34.0	31.0	35.0	2.0	35.0
94-95	29.818297225961295	34.0	31.0	35.0	2.0	35.0
96-97	29.63214644895378	34.0	31.0	35.0	2.0	35.0
98-99	29.37290404500649	34.0	31.0	35.0	2.0	35.0
100-101	28.296636508264143	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69286.0
3	23871.0
4	12770.0
5	9125.0
6	7940.0
7	7872.0
8	8113.0
9	8760.0
10	9702.0
11	10536.0
12	11381.0
13	12222.0
14	13326.0
15	14625.0
16	15941.0
17	17511.0
18	15948.0
19	13852.0
20	13076.0
21	12923.0
22	13690.0
23	15675.0
24	18264.0
25	21857.0
26	26570.0
27	32228.0
28	38258.0
29	46195.0
30	56628.0
31	70070.0
32	89521.0
33	119808.0
34	177008.0
35	259262.0
36	443879.0
37	871900.0
38	916387.0
39	117224.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35356761254509	19.149069519851405	13.510358994714739	25.987003872888764
2	15.08477874741058	20.556139140736317	38.33852704320309	26.02055506865002
3	18.207978162866606	24.459582280277434	28.956930459743134	28.375509097112822
4	13.222627803345432	16.431607731746244	35.209019455633154	35.13674500927518
5	14.216140511567671	37.70055656273706	33.86161628059262	14.221686645102642
6	33.91796444397598	34.93263525650316	16.92218716100783	14.227213138513022
7	29.212148660518228	32.13106609574001	20.265700483091788	18.391084760649978
8	27.10975623815241	35.35169877902231	19.244630876720283	18.293914106104996
9	27.320153008008326	15.57199039966974	18.941932494658552	38.16592409766338
10-11	26.202071818498545	25.369074611183713	27.110738594337473	21.318114975980272
12-13	26.492349988210904	22.478636208802325	27.34150802166339	23.68750578132338
14-15	23.705246485735696	24.12555826088866	24.612463320166565	27.55673193320908
16-17	23.962951810783977	27.249429828329536	25.312836156994905	23.47478220389158
18-19	23.951945992422853	26.72923541646509	26.623353007012398	22.69546558409966
20-21	24.32666598411222	25.72289240956451	26.85790451575644	23.092537090566832
22-23	24.257033855667036	25.430315263967046	26.44845837492908	23.86419250543684
24-25	24.063084617837067	25.66018283411405	26.354707623660207	23.922024924388683
26-27	23.649904538808737	25.9908481875412	26.48486379813255	23.874383475517508
28-29	23.750101050388768	26.460804321292063	26.094401444749494	23.694693183569683
30-31	24.47869626161297	25.845143731534957	26.0226085419198	23.653551464932274
32-33	24.086623800956104	25.9187293799589	25.988938406574913	24.00570841251008
34-35	24.35070407533562	26.0978392397919	26.16189724804491	23.389559436827575
36-37	24.252806850709867	26.00845496570377	26.275927008363148	23.462811175223212
38-39	24.29990118577075	26.111111111111114	26.023386034255598	23.565601668862538
40-41	24.462899571659207	25.888355249044714	25.948031168178076	23.700714011118002
42-43	24.075843122270022	25.865700510144695	25.862996611479684	24.195459756105596
44-45	23.891695493323923	25.94169939818477	26.132852287418075	24.033752821073236
46-47	24.069985180703593	25.843716471461974	25.82749306833827	24.25880527949616
48-49	23.814074835693937	26.063403343645398	26.03007751823072	24.092444302429943
50-51	24.05612443769057	25.91792176363954	26.099557179608524	23.92639661906137
52-53	24.284348512783858	25.814316131456604	25.934088888486308	23.967246467273227
54-55	23.563519851909433	25.69197864720233	26.388074581079806	24.35642691980843
56-57	23.783240421901215	25.828513454143586	25.989782281592937	24.39846384236226
58-59	23.93616145178797	25.853206531376845	26.168619260874603	24.042012755960588
60-61	24.26703442990225	25.85291730666318	26.19905413879814	23.680994124636435
62-63	24.10220220923733	25.803687223474714	26.20095296558101	23.89315760170695
64-65	23.6778189571319	25.597612802561283	26.291064278462432	24.43350396184438
66-67	23.798806364408957	25.83518108415721	26.031223442157277	24.334789109276556
68-69	23.724047590839696	25.771795754263632	26.16982753283309	24.334329122063583
70-71	23.662607632105352	25.631218112660832	26.078174619356524	24.62799963587729
72-73	23.624545844170427	26.072381587267245	25.85749754886954	24.445575019692782
74-75	23.540390730038695	26.012359362692216	25.94944511085016	24.497804796418926
76-77	23.5165058606505	25.53546250376432	26.047713114344763	24.900318521240415
78-79	23.46291377385986	25.56785946922738	26.179196017042273	24.790030739870488
80-81	23.713604968288383	25.88518509211385	25.815866780311346	24.58534315928642
82-83	23.674687904477075	25.893313936050188	25.97607451829264	24.455923641180092
84-85	23.62799604170488	25.90858743559808	25.780332742810764	24.68308377988628
86-87	23.278473977908096	26.216218146634883	25.726912328232	24.778395547225024
88-89	23.1670439663404	25.92498050431052	25.936732273371465	24.971243255977612
90-91	23.39088578454455	25.665914904983385	26.053099708933296	24.890099601538772
92-93	23.54681575169461	25.533127043814442	26.018074477061564	24.901982727429388
94-95	23.368220554102777	25.634030185632845	25.95727808034231	25.04047117992207
96-97	23.04846893432141	25.467846021721797	26.105219871811503	25.378465172145294
98-99	22.73620289827624	25.426816322234057	26.079737524962837	25.75724325452687
100-101	22.770827740639547	25.683983019071977	26.050177511414585	25.49501172887389
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	74.5
2	73.0
3	111.0
4	112.5
5	112.5
6	196.5
7	431.0
8	659.5
9	705.0
10	595.5
11	477.0
12	486.0
13	536.0
14	608.0
15	663.0
16	723.0
17	841.0
18	955.5
19	1074.5
20	1214.5
21	1416.5
22	1645.5
23	1956.0
24	2410.5
25	3114.5
26	4371.0
27	6383.0
28	9232.0
29	13342.0
30	18514.5
31	24974.5
32	33867.5
33	41204.0
34	47178.5
35	54714.0
36	64220.5
37	73418.0
38	81132.0
39	89477.5
40	96306.0
41	102230.0
42	108802.5
43	116391.5
44	124255.0
45	132158.5
46	139078.5
47	145055.5
48	153074.0
49	160213.0
50	162697.0
51	161693.0
52	160395.0
53	160148.0
54	157192.5
55	148921.5
56	137929.5
57	124923.5
58	110967.5
59	95343.0
60	78729.0
61	65366.5
62	54332.5
63	42097.0
64	30315.5
65	22401.5
66	16763.5
67	12549.5
68	9639.5
69	7596.0
70	5919.0
71	4304.5
72	3201.0
73	2602.5
74	2032.0
75	1516.5
76	1077.0
77	654.0
78	388.5
79	238.5
80	153.5
81	95.5
82	54.5
83	37.5
84	30.5
85	20.0
86	14.0
87	8.0
88	4.0
89	2.5
90	3.5
91	3.5
92	3.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14698584324584268
2	5.215183980711505E-4
3	0.006560152481000261
4	0.00411725051108803
5	0.028299235179545062
6	0.0015371068574728645
7	2.1958669392469496E-4
8	0.0017017968779163859
9	0.0
10-11	0.10155884594017142
12-13	3.019317041464556E-4
14-15	0.0054347706746361996
16-17	1.9213835718410807E-4
18-19	4.2544921947909646E-4
20-21	8.508984389581929E-4
22-23	1.9213835718410807E-4
24-25	0.0019076594034707873
26-27	0.0028683511893913276
28-29	0.006134703261521164
30-31	0.01410844508466165
32-33	0.01410844508466165
34-35	0.011418508084084136
36-37	6.450359134037914E-4
38-39	2.1958669392469496E-4
40-41	0.0054210465062659065
42-43	0.008783467756987797
44-45	0.008577605231433397
46-47	0.008852088598839266
48-49	0.010636230486977412
50-51	0.005379874001155026
52-53	0.0016194518676946252
54-55	8.783467756987798E-4
56-57	0.006107254924780578
58-59	0.009936297900092447
60-61	0.007809051802696964
62-63	0.01877466233056142
64-65	0.035147595196321484
66-67	0.03823553307963751
68-69	0.028450201031618286
70-71	0.04332719954501637
72-73	0.02775026844473332
74-75	0.026048471566816937
76-77	0.24035136066894888
78-79	0.06852477267287511
80-81	0.05584364109872399
82-83	0.021231288468843944
84-85	0.03392614421136537
86-87	0.08374487539553052
88-89	0.2665919705929499
90-91	0.32218857666100864
92-93	0.05175383892437654
94-95	0.005036769791897691
96-97	0.010142160425646847
98-99	0.0011665543114749417
100-101	0.0017017968779163859
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3643208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.44690241722946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70597393674164	41.97661749271831
2	13.18754181180448	14.624166879245129
3	4.551074450272983	7.57028942813497
4	2.0868278822400383	4.628325677924681
5	1.1342779098569138	3.1446098290927655
6	0.7215502370559999	2.400463534990369
7	0.4878750524316665	1.8935812296785524
8	0.33535568985918685	1.4875547368548003
9	0.26822347611148706	1.3384944815367332
>10	1.4053154201032707	13.919955228419715
>50	0.08464486570179969	3.184048476039817
>100	0.029253226959229935	2.759462744182372
>500	0.0014403615540773745	0.5646080154267781
>1k	6.456793072583634E-4	0.5078222457549728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7448336740586867E-5
2	0.0	0.0	0.0	0.0	2.7448336740586867E-5
3	0.0	0.0	0.0	0.0	2.7448336740586867E-5
4	0.0	0.0	0.0	2.7448336740586867E-5	2.7448336740586867E-5
5	0.0	0.0	0.0	2.7448336740586867E-5	2.7448336740586867E-5
6	0.0	0.0	0.0	2.7448336740586867E-5	8.23450102217606E-5
7	0.0	0.0	0.0	2.7448336740586867E-5	8.23450102217606E-5
8	0.0	0.0	0.0	2.7448336740586867E-5	8.23450102217606E-5
9	0.0	0.0	0.0	2.7448336740586867E-5	1.3724168370293432E-4
10-11	2.7448336740586867E-5	0.0	0.0	5.4896673481173734E-5	1.3724168370293432E-4
12-13	2.7448336740586867E-5	1.3724168370293433E-5	0.0	8.23450102217606E-5	2.744833674058687E-4
14-15	2.7448336740586867E-5	2.7448336740586867E-5	0.0	8.23450102217606E-5	3.7055254599792267E-4
16-17	2.7448336740586867E-5	2.7448336740586867E-5	0.0	8.23450102217606E-5	4.940700613305637E-4
18-19	2.7448336740586867E-5	2.7448336740586867E-5	0.0	8.23450102217606E-5	5.215183980711505E-4
20-21	2.7448336740586867E-5	2.7448336740586867E-5	0.0	1.0979334696234747E-4	5.215183980711505E-4
22-23	2.7448336740586867E-5	2.7448336740586867E-5	0.0	1.646900204435212E-4	5.489667348117374E-4
24-25	2.7448336740586867E-5	2.7448336740586867E-5	0.0	3.019317041464555E-4	6.03863408292911E-4
26-27	2.7448336740586867E-5	2.7448336740586867E-5	0.0	5.352425664414439E-4	6.03863408292911E-4
28-29	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.001962556076951961	6.03863408292911E-4
30-31	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.00783650013943755	6.03863408292911E-4
32-33	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.021766531035285384	6.587600817740848E-4
34-35	4.11725051108803E-5	2.7448336740586867E-5	0.0	0.041035263427177365	7.136567552552585E-4
36-37	5.4896673481173734E-5	4.11725051108803E-5	0.0	0.07192836642870788	7.136567552552585E-4
38-39	5.4896673481173734E-5	5.4896673481173734E-5	0.0	0.12336654948056769	7.411050919958454E-4
40-41	5.4896673481173734E-5	5.4896673481173734E-5	0.0	0.18553703219799694	7.411050919958454E-4
42-43	5.4896673481173734E-5	5.4896673481173734E-5	0.0	0.24842117167068145	7.548292603661389E-4
44-45	5.4896673481173734E-5	5.4896673481173734E-5	0.0	0.32719789811616573	7.960017654770192E-4
46-47	6.862084185146716E-5	5.4896673481173734E-5	0.0	0.41812051356935975	8.508984389581928E-4
48-49	8.23450102217606E-5	5.4896673481173734E-5	0.0	0.5140387263093406	9.057951124393667E-4
50-51	8.23450102217606E-5	5.4896673481173734E-5	0.0	0.6225282772765102	9.606917859205404E-4
52-53	8.23450102217606E-5	5.4896673481173734E-5	0.0	0.7367682547908327	0.0010018642910314206
54-55	8.23450102217606E-5	5.4896673481173734E-5	0.0	0.8721160032586666	0.0010842093012531813
56-57	8.23450102217606E-5	5.4896673481173734E-5	0.0	1.025305170607882	0.0011528301431046484
58-59	8.23450102217606E-5	5.4896673481173734E-5	0.0	1.196925896078401	0.0011802784798452353
60-61	8.23450102217606E-5	5.4896673481173734E-5	0.0	1.378016297724423	0.0011802784798452353
62-63	8.23450102217606E-5	8.23450102217606E-5	0.0	1.574916941333023	0.001262623490066996
64-65	8.23450102217606E-5	1.235175153326409E-4	0.0	1.7877101719144226	0.0013037959951778763
66-67	8.23450102217606E-5	1.646900204435212E-4	0.0	2.035843136049328	0.0013449685002887564
68-69	8.23450102217606E-5	1.646900204435212E-4	0.0	2.3107107801695648	0.0013724168370293434
70-71	8.23450102217606E-5	1.7841418881381463E-4	0.0	2.602102323007635	0.0014410376788808106
72-73	8.23450102217606E-5	1.9213835718410807E-4	0.0	2.913407634151001	0.001619451867694625
74-75	8.23450102217606E-5	1.9213835718410807E-4	0.0	3.24168974156842	0.0016743485411757988
76-77	8.23450102217606E-5	1.9213835718410807E-4	0.0	3.6093876605453215	0.0017017968779163859
78-79	8.23450102217606E-5	1.9213835718410807E-4	0.0	4.025778928900024	0.0017017968779163859
80-81	8.23450102217606E-5	1.9213835718410807E-4	0.0	4.463977900795124	0.001770417719767853
82-83	8.23450102217606E-5	1.9213835718410807E-4	0.0	4.925370717236019	0.0020449010871737214
84-85	8.23450102217606E-5	1.9213835718410807E-4	0.0	5.401050392950388	0.002058625255544015
86-87	8.23450102217606E-5	1.9213835718410807E-4	0.0	5.920235682398589	0.0020723494239143084
88-89	8.23450102217606E-5	1.9213835718410807E-4	0.0	6.481156167860853	0.0020997977606548954
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3450	0.0	51.105637	1
GTATCAA	6420	0.0	44.859203	1
TCAACGC	8265	0.0	33.993057	4
CAACGCA	8550	0.0	32.974762	5
ATCAACG	8705	0.0	32.438408	3
TATCAAC	8750	0.0	32.163548	2
AACGCAG	9050	0.0	31.04447	6
GTACATG	12460	0.0	26.012392	1
ACGCAGA	11000	0.0	25.281136	7
TACATGG	12940	0.0	24.939741	2
CGCAGAG	11330	0.0	24.209137	8
ACATGGG	13675	0.0	23.043692	3
CATGGGG	11510	0.0	21.06963	4
GCAGAGT	12570	0.0	20.913332	9
GAGTACA	9960	0.0	19.419992	1
ATGGGGG	7625	0.0	19.391602	5
AGAGTAC	10960	0.0	18.997522	10-11
GTATATG	1265	0.0	18.784231	1
TACCGTA	255	2.656834E-7	18.64203	7
CGTATAC	260	3.3154356E-7	18.253157	3
>>END_MODULE