FastQCFastQC Report
Mon 15 Aug 2016
SRR2921354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540742
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.058.6997681
GTATCAA29900.047.790581
TCAACGC38450.036.3039934
ATCAACG38800.035.851813
CAACGCA39650.035.3238565
TATCAAC39800.035.1895832
AACGCAG41850.033.458246
ACGCAGA50600.027.1131677
GTACATG60400.026.6446341
CGCAGAG50900.026.4887628
TCTATAC3050.026.4620973
GTAAGTA9550.025.353742
TACATGG64100.024.8860762
ACATGGG64700.024.5819153
GCAGAGT55250.022.9424659
CATGGGG56450.021.6158624
TGTAAGT10650.021.3962971
ATGGGGG37150.020.9591835
TAAGTAC12300.020.4571883
AGAGTAC48750.019.52544210-11
Produced by FastQC (version 0.11.5)