##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921354_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1540742
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171025389065786	33.0	31.0	34.0	28.0	34.0
2	31.245205881322118	34.0	31.0	34.0	28.0	34.0
3	31.28823579807651	34.0	31.0	34.0	28.0	34.0
4	34.71183559609591	37.0	35.0	37.0	32.0	37.0
5	34.7108892987924	37.0	35.0	37.0	32.0	37.0
6	34.76132214218863	37.0	35.0	37.0	32.0	37.0
7	34.69833430905369	37.0	35.0	37.0	32.0	37.0
8	34.67419983358668	37.0	35.0	37.0	32.0	37.0
9	36.34013157296939	39.0	37.0	39.0	32.0	39.0
10-11	36.253101752272606	39.0	37.0	39.0	32.0	39.0
12-13	36.16202355748075	39.0	37.0	39.0	32.0	39.0
14-15	37.42210701077792	40.0	38.0	41.0	32.0	41.0
16-17	37.36746807706936	40.0	38.0	41.0	32.0	41.0
18-19	37.32869390202902	40.0	38.0	41.0	32.0	41.0
20-21	37.2759313369792	40.0	38.0	41.0	32.0	41.0
22-23	37.17321329593145	40.0	38.0	41.0	31.0	41.0
24-25	37.08136404407746	40.0	38.0	41.0	31.0	41.0
26-27	36.95074905467625	40.0	38.0	41.0	31.0	41.0
28-29	36.8272241556341	40.0	38.0	41.0	30.0	41.0
30-31	36.69084213969633	40.0	37.0	41.0	30.0	41.0
32-33	36.53100356841055	40.0	37.0	41.0	30.0	41.0
34-35	36.38431937339282	40.0	37.0	41.0	30.0	41.0
36-37	36.229517985490105	40.0	36.5	41.0	29.5	41.0
38-39	36.46405855100984	40.0	37.0	41.0	30.0	41.0
40-41	36.59141666807291	40.0	37.0	41.0	30.0	41.0
42-43	36.4510602683642	40.0	37.0	41.0	30.0	41.0
44-45	36.34617801033528	40.0	37.0	41.0	29.5	41.0
46-47	36.201449691122846	40.0	36.5	41.0	29.0	41.0
48-49	36.06345189525566	40.0	36.0	41.0	28.0	41.0
50-51	34.38479966146181	38.0	34.0	39.5	25.5	40.0
52-53	34.55896412248124	38.5	34.5	39.5	25.5	40.5
54-55	35.39382940167789	39.0	35.0	41.0	27.0	41.0
56-57	35.390841231043225	39.0	35.0	41.0	27.0	41.0
58-59	35.213873575199486	39.0	35.0	41.0	26.5	41.0
60-61	34.96639541208067	39.0	35.0	41.0	26.0	41.0
62-63	34.67833225809383	38.0	35.0	40.5	26.0	41.0
64-65	34.38162716405472	37.0	35.0	40.0	26.0	41.0
66-67	34.039789919402466	37.0	34.0	40.0	26.0	41.0
68-69	33.668285799958724	36.0	34.0	39.0	25.0	41.0
70-71	33.28943229950245	36.0	34.0	39.0	24.0	41.0
72-73	32.935521651256344	35.0	34.0	38.5	23.5	40.0
74-75	32.538164728423055	35.0	34.0	37.0	22.0	39.5
76-77	32.17511140736087	35.0	33.0	37.0	21.5	39.0
78-79	31.84445903337483	35.0	33.0	36.0	20.0	39.0
80-81	31.51656961386137	35.0	33.0	36.0	19.0	37.0
82-83	31.234296851776612	35.0	33.0	35.5	18.0	37.0
84-85	31.026504437472333	35.0	33.0	35.0	16.0	36.5
86-87	30.82490546762534	35.0	33.0	35.0	12.0	36.0
88-89	30.64831263118679	35.0	33.0	35.0	7.0	36.0
90-91	30.43064088601466	35.0	32.0	35.0	2.0	36.0
92-93	30.271653852494445	35.0	32.0	35.0	2.0	35.0
94-95	30.106482136529024	35.0	32.0	35.0	2.0	35.0
96-97	29.89394752658135	35.0	32.0	35.0	2.0	35.0
98-99	29.712700114620098	35.0	32.0	35.0	2.0	35.0
100-101	28.73668368876814	34.0	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40822.0
3	6742.0
4	4806.0
5	3596.0
6	3144.0
7	3245.0
8	3362.0
9	3551.0
10	3807.0
11	4046.0
12	3992.0
13	4249.0
14	4440.0
15	4944.0
16	5116.0
17	5479.0
18	5106.0
19	4615.0
20	4498.0
21	4709.0
22	5372.0
23	6095.0
24	7188.0
25	8398.0
26	9978.0
27	11805.0
28	14354.0
29	17041.0
30	20474.0
31	25507.0
32	32948.0
33	43553.0
34	66772.0
35	101437.0
36	179755.0
37	366198.0
38	421071.0
39	78520.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.855733040907765	18.152135310452202	14.00545765834903	24.986673990291003
2	16.159195387372414	19.646585980313223	39.224670804872346	24.969547827442018
3	19.210297709556908	23.675616435510115	29.28089773838385	27.833188116549127
4	14.040306054868596	15.978276960956187	35.93021564609419	34.05120133808103
5	15.078430354105258	36.797145862279415	33.37717988339328	14.747243900222045
6	33.543582783043206	34.06346170947927	17.550344000295997	14.842611507181521
7	29.16638539389439	31.430674483453373	20.923903166006326	18.479036956645913
8	27.376250124144214	33.168997765079546	20.25747997302257	19.197272137753668
9	27.382494832940395	15.24983641972103	19.397700009347442	37.96996873799113
10-11	25.889348525691062	24.74229414122661	27.481040658111905	21.88731667497042
12-13	26.105030074111724	22.273051706828774	27.540881868837207	24.08103635022229
14-15	23.812284803621434	23.748362997942426	24.86490066311163	27.574451535324506
16-17	23.93689986282579	26.585796853627393	25.76778282199747	23.70952046154935
18-19	23.827935573540515	26.182970521733317	26.997973933133952	22.99111997159222
20-21	24.337315999554658	25.16295454995461	26.869153038837407	23.63057641165333
22-23	24.222328126562324	24.92439165964365	26.572679450503646	24.28060076329038
24-25	24.03991644037287	25.23241035762806	26.333391225589892	24.394281976409175
26-27	23.657527274049336	25.596932269985174	26.441057929275747	24.304482526689743
28-29	23.771403049391864	25.955535733934994	26.19160993647452	24.081451280198625
30-31	24.34999926972592	25.30636620486298	26.156113124323483	24.187521401087622
32-33	24.010771344873223	25.41457085012096	26.018081630877944	24.55657617412787
34-35	24.19673478316326	25.689654780709624	26.13423005683281	23.97938037929431
36-37	24.189355748841937	25.624724560416272	26.150420680214886	24.0354990105269
38-39	24.078237256847316	25.622484172783516	26.084213416468614	24.215065153900554
40-41	24.17462340382514	25.371565913782625	26.136652396951852	24.317158285440378
42-43	23.935455419072774	25.489045249908436	25.95383850920373	24.62166082181506
44-45	23.82922298674896	25.450195128148394	26.229700482922265	24.490881402180378
46-47	23.995742062019545	25.39617604415986	25.963738641112105	24.644343252708488
48-49	23.71925448804785	25.555480099026408	26.095095372539895	24.630170040385853
50-51	23.848261377017476	25.53379117808376	26.24265338535642	24.375294059542345
52-53	24.103387455677908	25.329296253548367	26.20777703257739	24.35953925819634
54-55	23.538303519990176	25.175294399384963	26.56153281016605	24.724869270458814
56-57	23.694092116051237	25.359650100561314	26.24525262517195	24.701005158215498
58-59	23.89117115852426	25.36487609778036	26.324176741906825	24.419776001788556
60-61	23.98951420836465	25.337381028282195	26.428822343692065	24.244282419661086
62-63	23.98097541303508	25.268280667251382	26.411432690092322	24.339311229621213
64-65	23.615252154759904	25.058907982011252	26.54844882959077	24.777391033638075
66-67	23.66622160541144	25.279035210313378	26.284046129965468	24.77069705430971
68-69	23.769425927057164	25.235704064351694	26.45107142346539	24.543798585125753
70-71	23.630065886537658	25.107452661209855	26.428302457513595	24.83417899473889
72-73	23.551301600442667	25.451902404091868	26.238688427860836	24.758107567604625
74-75	23.58147415892428	25.46833649851921	26.323992650487128	24.62619669206938
76-77	23.52273391860367	25.008545120184028	26.415240855421768	25.053480105790538
78-79	23.59623591686155	25.038965462106333	26.47948512292807	24.88531349810405
80-81	23.72065132605304	25.4001885075403	26.178854654186168	24.70030551222049
82-83	23.65089857762854	25.369990126663406	26.43490000240496	24.544211293303096
84-85	23.661972837950305	25.44679982620344	26.240323466987913	24.65090386885834
86-87	23.385776252932487	25.630358346588693	26.266258269299147	24.71760713117967
88-89	23.36127166878577	25.2285509247342	26.474367974789192	24.935809431690835
90-91	23.53458138325533	25.09464378575143	26.498602444097763	24.872172386895475
92-93	23.697368262814237	24.964076034969352	26.54505552653758	24.793500175678833
94-95	23.5209138577266	25.047477561529035	26.522614012788548	24.908994567955816
96-97	23.279535095848935	24.883372499022347	26.6711028080645	25.165989597064215
98-99	23.080028460515585	24.818960762128143	26.655990181187132	25.445020596169144
100-101	23.338027445603235	24.863548917916102	26.65303293615422	25.14539070032644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	39.0
2	41.5
3	49.5
4	50.5
5	53.0
6	87.5
7	184.5
8	319.5
9	392.5
10	327.0
11	273.5
12	278.0
13	296.5
14	337.5
15	380.5
16	411.0
17	456.5
18	504.5
19	535.0
20	585.0
21	631.5
22	694.0
23	776.0
24	865.5
25	1034.0
26	1271.0
27	1652.5
28	2204.0
29	3161.5
30	4852.5
31	7383.0
32	10886.0
33	14342.0
34	17651.0
35	21369.5
36	26210.5
37	31276.5
38	35331.5
39	38673.5
40	41478.5
41	44372.0
42	47266.0
43	50675.5
44	54511.5
45	58607.5
46	61815.0
47	64745.5
48	67933.5
49	68794.5
50	68291.0
51	67933.5
52	67081.0
53	66657.0
54	65871.0
55	63081.5
56	58311.5
57	52084.0
58	46796.5
59	40941.0
60	33767.0
61	27859.5
62	23095.5
63	17978.0
64	13228.0
65	9923.0
66	7605.5
67	5948.5
68	4457.5
69	3435.5
70	2695.5
71	1932.5
72	1381.5
73	1064.5
74	813.5
75	617.5
76	483.0
77	320.5
78	200.5
79	150.0
80	114.0
81	80.0
82	73.5
83	65.0
84	49.0
85	41.0
86	31.0
87	30.0
88	31.5
89	21.5
90	17.0
91	19.0
92	17.0
93	12.5
94	10.5
95	9.5
96	8.0
97	5.5
98	4.5
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033814876208995405
2	0.04037015931285056
3	0.03349035724345802
4	0.04062977448528047
5	0.03387978000210288
6	0.012396624483528067
7	0.008177877931542074
8	0.012656239655957973
9	0.013889411725000031
10-11	0.01781609120800238
12-13	0.019308878449474345
14-15	0.03757929620922906
16-17	0.023689884484229026
18-19	0.020704310001285097
20-21	0.023268009829030427
22-23	0.03686535448504682
24-25	0.03404203948487158
26-27	0.029206706898364552
28-29	0.021840126380665938
30-31	0.014570901552628539
32-33	0.011228356207593484
34-35	0.011066096724824793
36-37	0.001492787241471966
38-39	0.007820907069450952
40-41	0.004316102241647206
42-43	0.14334002707786248
44-45	0.12211648673171759
46-47	0.1277955686286218
48-49	0.12724388638720824
50-51	0.12578355104229003
52-53	0.13019700897359845
54-55	0.12808763569760545
56-57	0.13814772362926434
58-59	0.13428594793936946
60-61	0.13555157190496528
62-63	0.13639532121536246
64-65	0.13717416673265218
66-67	0.12996984569772227
68-69	0.1264325889733648
70-71	0.11737850983487177
72-73	0.12244100569725495
74-75	0.12101312224889046
76-77	0.12020182483504702
78-79	0.14278834483644892
80-81	0.15200468345771065
82-83	0.14635805345736014
84-85	0.14028954880181108
86-87	0.15388689345782747
88-89	0.15278352897500036
90-91	0.15200468345771065
92-93	0.15729434259596997
94-95	0.17180034035549105
96-97	0.16995058225192797
98-99	0.1603448208720214
100-101	0.1716705327692761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1540742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.74065066666083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2397364801691	51.145330125673794
2	11.167625086301074	14.236633788043093
3	3.5385077202557973	6.766403534343215
4	1.6380637601580281	4.17644999623799
5	0.9159775318273305	2.9192501937358037
6	0.5638593960278762	2.1564458872396326
7	0.39666092053073476	1.7698397618065975
8	0.2699298564363972	1.376440374689144
9	0.21232050132338937	1.2180102213802013
>10	1.0092381524339051	10.845967920714124
>50	0.03499185788572621	1.4783890918927647
>100	0.011759497380225541	1.3717173838203822
>500	0.0013292392703735034	0.5391217204232879
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.490379310747679E-5
2	0.0	0.0	0.0	0.0	6.490379310747679E-5
3	0.0	0.0	0.0	0.0	6.490379310747679E-5
4	0.0	0.0	0.0	0.0	6.490379310747679E-5
5	0.0	0.0	0.0	0.0	6.490379310747679E-5
6	0.0	0.0	0.0	0.0	1.2980758621495357E-4
7	0.0	0.0	0.0	0.0	1.9471137932243037E-4
8	0.0	0.0	0.0	0.0	1.9471137932243037E-4
9	0.0	0.0	0.0	0.0	2.5961517242990714E-4
10-11	0.0	0.0	0.0	0.0	2.5961517242990714E-4
12-13	0.0	0.0	0.0	0.0	4.867784483060759E-4
14-15	0.0	0.0	0.0	0.0	5.516822414135527E-4
16-17	0.0	0.0	0.0	0.0	5.841341379672911E-4
18-19	0.0	0.0	0.0	0.0	7.139417241822446E-4
20-21	0.0	0.0	0.0	0.0	7.46393620735983E-4
22-23	0.0	0.0	0.0	0.0	8.762012069509367E-4
24-25	0.0	0.0	0.0	6.490379310747679E-5	0.0010384606897196286
26-27	0.0	0.0	0.0	2.9206706898364553E-4	0.001070912586273367
28-29	6.490379310747679E-5	0.0	0.0	5.841341379672911E-4	0.0011033644828271055
30-31	6.490379310747679E-5	0.0	0.0	0.0027908631036215018	0.0011033644828271055
32-33	6.490379310747679E-5	0.0	0.0	0.008729560172955628	0.0011033644828271055
34-35	6.490379310747679E-5	0.0	0.0	0.018108158276986024	0.0011033644828271055
36-37	6.490379310747679E-5	0.0	0.0	0.03336054965724307	0.0011033644828271055
38-39	6.490379310747679E-5	0.0	0.0	0.05844586569328285	0.0011033644828271055
40-41	6.490379310747679E-5	0.0	0.0	0.08817180293650723	0.0011682682759345821
42-43	6.490379310747679E-5	0.0	0.0	0.12289533224900728	0.0011682682759345821
44-45	6.490379310747679E-5	0.0	0.0	0.16836043932079478	0.0012007201724883207
46-47	6.490379310747679E-5	0.0	0.0	0.22446976846220845	0.0013629796552570126
48-49	6.490379310747679E-5	0.0	0.0	0.287426447776461	0.0016225948276869196
50-51	6.490379310747679E-5	0.0	0.0	0.3523302408839377	0.0016874986207943965
52-53	6.490379310747679E-5	0.0	0.0	0.42385422088837715	0.0017199505173481349
54-55	6.490379310747679E-5	0.0	0.0	0.5176726538252349	0.0017848543104556115
56-57	6.490379310747679E-5	0.0	0.0	0.630410542452922	0.0018497581035630884
58-59	6.490379310747679E-5	0.0	0.0	0.7481135712533311	0.0018822100001168267
60-61	6.490379310747679E-5	0.0	0.0	0.8778238017786235	0.0018822100001168267
62-63	6.490379310747679E-5	0.0	0.0	1.0167503709251777	0.0018822100001168267
64-65	6.490379310747679E-5	0.0	0.0	1.1798536030042668	0.0018822100001168267
66-67	6.490379310747679E-5	0.0	0.0	1.3726827723265802	0.0018822100001168267
68-69	6.490379310747679E-5	0.0	0.0	1.5831333214775738	0.0018822100001168267
70-71	6.490379310747679E-5	0.0	0.0	1.8076679937328897	0.0018822100001168267
72-73	6.490379310747679E-5	0.0	0.0	2.0433985702992454	0.0021742770691004724
74-75	6.490379310747679E-5	0.0	0.0	2.3003202353151924	0.0022391808622079495
76-77	6.490379310747679E-5	0.0	0.0	2.612345220679387	0.0022716327587616876
78-79	6.490379310747679E-5	0.0	0.0	2.9592235429422966	0.0022716327587616876
80-81	6.490379310747679E-5	0.0	0.0	3.3267088195168304	0.0022716327587616876
82-83	6.490379310747679E-5	0.0	0.0	3.710906822816539	0.0022716327587616876
84-85	6.490379310747679E-5	0.0	0.0	4.125642060773316	0.0023040846553154257
86-87	6.490379310747679E-5	0.0	0.0	4.584446974250069	0.002368988448422903
88-89	6.490379310747679E-5	0.0	0.0	5.084368440660409	0.002401440344976641
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1480	0.0	58.699768	1
GTATCAA	2990	0.0	47.79058	1
TCAACGC	3845	0.0	36.303993	4
ATCAACG	3880	0.0	35.85181	3
CAACGCA	3965	0.0	35.323856	5
TATCAAC	3980	0.0	35.189583	2
AACGCAG	4185	0.0	33.45824	6
ACGCAGA	5060	0.0	27.113167	7
GTACATG	6040	0.0	26.644634	1
CGCAGAG	5090	0.0	26.488762	8
TCTATAC	305	0.0	26.462097	3
GTAAGTA	955	0.0	25.35374	2
TACATGG	6410	0.0	24.886076	2
ACATGGG	6470	0.0	24.581915	3
GCAGAGT	5525	0.0	22.942465	9
CATGGGG	5645	0.0	21.615862	4
TGTAAGT	1065	0.0	21.396297	1
ATGGGGG	3715	0.0	20.959183	5
TAAGTAC	1230	0.0	20.457188	3
AGAGTAC	4875	0.0	19.525442	10-11
>>END_MODULE