FastQCFastQC Report
Mon 15 Aug 2016
SRR2921354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540742
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.055.3474731
GTATCAA26350.042.9115751
TATCAAC41150.031.0570761
TCAACGC44150.028.8119873
ATCAACG44200.028.5646212
CAACGCA45600.027.9999054
AACGCAG49750.025.9799675
TATACCG2103.4338882E-822.6278615
ACGCAGA63800.022.48871
TTATATG7150.021.90672
CGCAGAG65350.021.498852
TAAGGTG6950.020.5115855
TATGTAA9650.019.6744142
ATTATAT9450.019.6069891
ATATGTA10050.018.9091511
GTACATG57750.018.8391631
AGAGTAC60250.018.7707985
TATATGT7900.018.6253193
CAGAGTA63100.018.5989329
ATGTAAG10800.018.4584263
Produced by FastQC (version 0.11.5)