##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1540742
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.62024076711091	31.0	31.0	34.0	25.0	34.0
2	29.81063474611583	31.0	31.0	34.0	25.0	34.0
3	29.85541122394275	31.0	31.0	34.0	25.0	34.0
4	33.33931443421417	37.0	35.0	37.0	28.0	37.0
5	32.94386341126548	37.0	35.0	37.0	26.0	37.0
6	32.86989969767813	37.0	35.0	37.0	25.0	37.0
7	32.67087091803819	37.0	35.0	37.0	23.0	37.0
8	32.601058451057995	37.0	35.0	37.0	23.0	37.0
9	34.021192386525456	39.0	35.0	39.0	23.0	39.0
10-11	33.810113244138215	38.5	35.0	39.0	20.0	39.0
12-13	33.70148473917112	38.0	35.0	39.0	18.0	39.0
14-15	34.68994354668075	39.0	35.0	41.0	17.0	41.0
16-17	34.628359582590726	39.0	35.0	41.0	17.0	41.0
18-19	34.55778903930704	39.0	35.0	41.0	17.5	41.0
20-21	34.49635078423253	39.0	34.5	41.0	18.0	41.0
22-23	34.35885242305331	39.0	34.0	41.0	16.5	41.0
24-25	34.24441113437551	39.0	34.0	41.0	16.0	41.0
26-27	34.05716628741217	39.0	34.0	41.0	11.0	41.0
28-29	33.870933939621295	39.0	34.0	40.5	10.0	41.0
30-31	33.68706993124092	39.0	34.0	40.0	9.0	41.0
32-33	33.620426716478164	38.5	33.5	40.0	9.0	41.0
34-35	33.744166122556535	39.0	34.0	40.5	8.0	41.0
36-37	33.699727793491704	39.0	34.0	41.0	8.0	41.0
38-39	33.59066410859184	39.0	34.0	41.0	4.0	41.0
40-41	33.4396031262859	39.0	33.0	41.0	2.0	41.0
42-43	33.29741222086501	39.0	33.0	40.5	2.0	41.0
44-45	33.132185012156484	38.0	33.0	40.0	2.0	41.0
46-47	32.95074061718315	38.0	33.0	40.0	2.0	41.0
48-49	32.77217210928241	38.0	33.0	40.0	2.0	41.0
50-51	32.598278621599206	38.0	32.5	40.0	2.0	41.0
52-53	32.40585672357864	38.0	32.0	40.0	2.0	41.0
54-55	32.17734701851445	38.0	32.0	40.0	2.0	41.0
56-57	31.903006473504327	37.0	31.5	40.0	2.0	41.0
58-59	31.655378707142404	37.0	31.0	40.0	2.0	41.0
60-61	31.370768759467843	36.0	31.0	40.0	2.0	41.0
62-63	31.082624151220646	36.0	31.0	39.0	2.0	41.0
64-65	30.759702468031634	35.5	30.5	39.0	2.0	40.5
66-67	30.433529753845875	35.0	30.0	39.0	2.0	40.0
68-69	30.086679015695033	35.0	30.0	38.0	2.0	40.0
70-71	29.725226222170875	35.0	29.0	37.0	2.0	39.5
72-73	29.34487247053692	35.0	29.0	37.0	2.0	39.0
74-75	28.943517798567186	34.5	29.0	36.0	2.0	39.0
76-77	26.451798549010803	31.0	25.0	34.0	2.0	36.0
78-79	27.981190231719523	34.0	27.5	35.0	2.0	37.0
80-81	28.208140298635335	34.0	29.0	35.0	2.0	37.0
82-83	28.064655211579876	34.0	29.0	35.0	2.0	36.0
84-85	27.844399646404135	34.0	28.5	35.0	2.0	36.0
86-87	27.59519406883177	34.0	27.5	35.0	2.0	35.5
88-89	27.306040855639687	34.0	27.0	35.0	2.0	35.0
90-91	27.09055312310562	34.0	26.5	35.0	2.0	35.0
92-93	26.922106361739992	34.0	26.0	35.0	2.0	35.0
94-95	26.78532486295564	34.0	26.0	35.0	2.0	35.0
96-97	26.55518152941894	34.0	25.0	35.0	2.0	35.0
98-99	26.219097681506703	33.5	25.0	35.0	2.0	35.0
100-101	25.091963482529845	32.0	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87282.0
3	20372.0
4	10590.0
5	7005.0
6	6016.0
7	5769.0
8	5850.0
9	5948.0
10	6544.0
11	6803.0
12	7035.0
13	7560.0
14	7924.0
15	8442.0
16	8872.0
17	9507.0
18	8605.0
19	7584.0
20	7244.0
21	7275.0
22	7762.0
23	8743.0
24	10267.0
25	12381.0
26	14724.0
27	17569.0
28	20687.0
29	25086.0
30	30148.0
31	37350.0
32	46869.0
33	61953.0
34	89211.0
35	133100.0
36	220527.0
37	331914.0
38	216407.0
39	13817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.397564681647477	26.09515593300276	16.197972257067445	27.309307128282317
2	12.384657472365499	26.073820515766155	32.43311480155276	29.108407210315583
3	14.466653036454327	28.92408945313641	28.06902079905497	28.54023671135429
4	12.660527473839847	21.47748234246467	31.78116209364303	34.08082809005246
5	14.318422745576129	38.538504033564244	33.17130265266815	13.971770568191483
6	33.28973910966533	36.90472790713976	16.22217866405664	13.583354319138275
7	28.155654872782076	32.79030493100078	20.94237711440332	18.111663081813827
8	27.646458267482473	35.27161493033493	18.959985824891984	18.12194097729062
9	26.880295338220154	15.349098031987186	18.974429203591516	38.79617742620114
10-11	26.018752991652278	26.302866595527313	26.943120774747825	20.735259638072588
12-13	26.372887824320724	23.489824204545933	27.152188932652145	22.985099038481195
14-15	24.004792676499527	24.790677387446095	24.38566247199296	26.81886746406142
16-17	24.109801890457074	27.888931184623477	25.034715495199215	22.966551429720226
18-19	24.232735825911547	27.160820751667714	26.449763554254012	22.15667986816673
20-21	24.81997987318353	25.883793434348192	26.450927011570162	22.84529968089812
22-23	24.67024784511084	25.66324439449732	26.114845426598443	23.551662333793395
24-25	24.343676424534593	26.151269939914553	25.865397565103386	23.63965607044747
26-27	24.063734831530684	26.429126836183126	26.003799581487847	23.503338750798342
28-29	24.260772710367	26.668036237147298	25.853693523878885	23.217497528606824
30-31	24.729899515836344	26.234678996930526	25.731430520713683	23.303990966519454
32-33	24.653612302187625	26.28018771748123	25.429493481527015	23.636706498804127
34-35	24.715648531654	26.505977173991358	25.615486323283427	23.162887971071218
36-37	24.706340882126156	26.379376815695398	25.729912794697583	23.18436950748086
38-39	24.84061219973013	26.21294067327432	25.619556589402876	23.326890537592675
40-41	24.847930580468194	26.236944325538087	25.503460688801532	23.411664405192184
42-43	24.56502760261324	26.333412306124504	25.43416103309393	23.66739905816833
44-45	24.6065877943634	26.132268028221628	25.426078230342046	23.835065947072927
46-47	24.69611817994166	26.132019011194586	25.397581148746628	23.774281660117126
48-49	24.2621360987921	26.358754920390933	25.62277349064736	23.756335490169604
50-51	24.541877510326906	26.177046362671003	25.51280769760021	23.768268429401886
52-53	24.774235506433886	26.045889896829998	25.48072819697678	23.699146399759332
54-55	24.118388113678655	26.02294108827704	25.819887858280072	24.03878293976423
56-57	24.47219260795415	25.99181834851369	25.4829722015328	24.053016841999362
58-59	24.55203315332765	25.99189471371372	25.71602807867976	23.74004405427887
60-61	24.644999815011104	26.06860297033752	25.777553771728893	23.508843442922487
62-63	24.627954696972548	26.06076323410178	25.679678487942976	23.6316035809827
64-65	24.226158246889245	25.80493955081536	25.814580517327123	24.154321684968274
66-67	24.395414047805936	26.198930186869955	25.503012785429043	23.90264297989507
68-69	24.356888212433088	25.944546854139706	25.597522431943	24.10104250148421
70-71	24.298144921062402	25.825669846332044	25.65351732627626	24.222667906329292
72-73	24.319707245441617	26.206875733168527	25.346126955089026	24.127290066300834
74-75	24.279115814074267	26.13863282264347	25.290119449493638	24.292131913788626
76-77	24.101843813908424	25.762121670259358	25.75796000247099	24.37807451336123
78-79	24.097548875734727	25.745992929998675	25.80031012423151	24.35614807003509
80-81	24.36612941556898	25.995836531256533	25.457124453431096	24.180909599743387
82-83	24.306534491751837	25.96405492350043	25.614640294436597	24.114770290311135
84-85	24.14313269375722	26.01846014811487	25.56598071403391	24.272426444094005
86-87	24.064308314116634	26.209141985345624	25.368186109224894	24.358363591312852
88-89	24.044644974041766	26.002627208027196	25.45506996185377	24.497657856077264
90-91	23.935896768483932	25.888569309056837	25.61636971771412	24.55916420474511
92-93	24.149515878019308	25.776111195154428	25.55773202502774	24.51664090179852
94-95	24.008041851378277	25.91884155800323	25.5674081093536	24.505708481264886
96-97	23.721118617763405	25.87396678054839	25.623290678801936	24.78162392288627
98-99	23.541075430030844	25.69131890188595	25.56624852140135	25.20135714668185
100-101	23.594864229326102	25.679048083269997	25.62910399857729	25.096983688826608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	41.0
2	29.5
3	46.0
4	47.0
5	54.0
6	94.0
7	194.0
8	282.5
9	301.5
10	270.0
11	216.0
12	208.5
13	227.5
14	247.5
15	265.5
16	315.5
17	365.0
18	396.0
19	425.0
20	476.5
21	555.0
22	647.0
23	756.5
24	869.5
25	1090.5
26	1428.0
27	1946.0
28	2683.5
29	3851.0
30	5819.0
31	8733.0
32	12710.5
33	16024.0
34	18946.0
35	22830.5
36	27487.0
37	32262.0
38	36169.0
39	39316.0
40	42266.5
41	45128.0
42	48279.0
43	51710.0
44	55160.5
45	59430.0
46	62832.5
47	65739.5
48	68615.5
49	69192.5
50	68256.5
51	67407.0
52	66440.5
53	65411.5
54	64028.0
55	60863.5
56	56578.0
57	50882.0
58	44686.5
59	38833.0
60	32306.0
61	26802.0
62	22505.5
63	17544.0
64	12730.0
65	9468.0
66	7196.0
67	5578.5
68	4346.0
69	3289.0
70	2409.5
71	1755.0
72	1262.5
73	943.5
74	728.0
75	523.5
76	367.0
77	248.0
78	139.5
79	76.0
80	51.5
81	29.0
82	17.5
83	6.5
84	3.5
85	5.0
86	5.5
87	3.0
88	1.0
89	1.5
90	1.0
91	0.5
92	1.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09054079138493011
2	3.245189655373839E-4
3	0.003050478276051409
4	0.0025961517242990713
5	0.0190168113804907
6	0.0011682682759345821
7	0.0
8	8.437493103971983E-4
9	0.0
10-11	0.06756484862488334
12-13	1.6225948276869196E-4
14-15	0.003764420000233654
16-17	2.2716327587616876E-4
18-19	5.192303448598143E-4
20-21	7.46393620735983E-4
22-23	9.735568966121517E-5
24-25	0.0012656239655957973
26-27	0.002985574482943932
28-29	0.007334128621144877
30-31	0.016258400173422935
32-33	0.015284843276810784
34-35	0.00850239689707946
36-37	6.490379310747678E-4
38-39	1.9471137932243035E-4
40-41	0.005224755345151881
42-43	0.008242781724649552
44-45	0.009768020862675256
46-47	0.011423067586915915
48-49	0.013889411725000031
50-51	0.0068798020693925386
52-53	0.0021093732759929957
54-55	8.437493103971983E-4
56-57	0.006457927414193941
58-59	0.009248790517815441
60-61	0.007074513448714971
62-63	0.01758892793212621
64-65	0.028557668967289784
66-67	0.030699494139836518
68-69	0.02339781741524538
70-71	0.03491824069182251
72-73	0.022002385863434632
74-75	0.02219709724275706
76-77	0.18802628863236026
78-79	0.046341308278738426
80-81	0.04413457931308421
82-83	0.02002282017365659
84-85	0.02927161069147203
86-87	0.06915499155601652
88-89	0.21862842708253558
90-91	0.2597449800161221
92-93	0.034463914140070176
94-95	0.003374997241588793
96-97	0.007042061552161231
98-99	6.165860345210295E-4
100-101	0.001233172069042059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1540742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.25016770267891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83661507143665	55.70776255476374
2	9.760545108345992	13.127965908116607
3	3.0288228102318553	6.110665257893745
4	1.4475395513250187	3.8938911033147234
5	0.7577482208359138	2.5479347463810886
6	0.5052441187551092	2.038665103024399
7	0.3550954834333287	1.6716161567948635
8	0.23496643124701777	1.2641225524689537
9	0.18671658222073706	1.130104932049404
>10	0.8485607028811107	9.637979099823136
>50	0.027943874435000002	1.2680301950549953
>100	0.00923050197784367	1.1620709667555331
>500	9.71542874456592E-4	0.4391914235588013
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	3.245189655373839E-5
14-15	0.0	0.0	0.0	0.0	6.490379310747679E-5
16-17	0.0	0.0	0.0	0.0	1.2980758621495357E-4
18-19	0.0	0.0	0.0	0.0	1.9471137932243037E-4
20-21	0.0	0.0	0.0	0.0	3.245189655373839E-4
22-23	0.0	0.0	0.0	0.0	3.8942275864486074E-4
24-25	0.0	0.0	0.0	9.735568966121519E-5	4.543265517523375E-4
26-27	0.0	0.0	0.0	2.2716327587616876E-4	5.841341379672911E-4
28-29	0.0	0.0	0.0	5.192303448598143E-4	5.841341379672911E-4
30-31	0.0	0.0	0.0	0.0024014403449766413	5.841341379672911E-4
32-33	0.0	0.0	0.0	0.007820907069450952	5.841341379672911E-4
34-35	0.0	0.0	0.0	0.016420659656191626	6.490379310747678E-4
36-37	0.0	0.0	0.0	0.030634590346729045	6.490379310747678E-4
38-39	0.0	0.0	0.0	0.05354562931366835	6.490379310747678E-4
40-41	0.0	0.0	0.0	0.08035089586705627	6.490379310747678E-4
42-43	0.0	0.0	0.0	0.11199149500695119	6.490379310747678E-4
44-45	0.0	0.0	0.0	0.15356237449229007	6.490379310747678E-4
46-47	0.0	0.0	0.0	0.2038952660471383	7.139417241822446E-4
48-49	0.0	0.0	0.0	0.26065363311962675	7.139417241822446E-4
50-51	0.0	0.0	0.0	0.31945646967500074	7.139417241822446E-4
52-53	0.0	0.0	0.0	0.3832244464030967	8.112974138434598E-4
54-55	0.0	0.0	0.0	0.4678914445118002	9.411050000584134E-4
56-57	0.0	0.0	0.0	0.5699526591733074	9.735568966121518E-4
58-59	0.0	0.0	0.0	0.6760054571109245	9.735568966121518E-4
60-61	0.0	0.0	0.0	0.792410410049184	9.735568966121518E-4
62-63	0.0	0.0	0.0	0.9191350660915325	9.735568966121518E-4
64-65	0.0	0.0	0.0	1.0674726852386707	0.0010060087931658902
66-67	0.0	0.0	0.0	1.2403114862838813	0.0010384606897196286
68-69	0.0	3.245189655373839E-5	0.0	1.431647868364723	0.0010384606897196286
70-71	6.490379310747679E-5	6.490379310747679E-5	0.0	1.6372306330326558	0.0011033644828271055
72-73	6.490379310747679E-5	6.490379310747679E-5	0.0	1.8523218033908337	0.0011033644828271055
74-75	6.490379310747679E-5	6.490379310747679E-5	0.0	2.0885067065089418	0.0011033644828271055
76-77	6.490379310747679E-5	6.490379310747679E-5	0.0	2.3747324341129143	0.0011033644828271055
78-79	6.490379310747679E-5	6.490379310747679E-5	0.0	2.6954220758569574	0.0011033644828271055
80-81	6.490379310747679E-5	6.490379310747679E-5	0.0	3.036848479498839	0.0011033644828271055
82-83	6.490379310747679E-5	6.490379310747679E-5	0.0	3.390152277279389	0.001233172069042059
84-85	1.2980758621495357E-4	6.490379310747679E-5	0.0	3.7714620617858152	0.001233172069042059
86-87	1.2980758621495357E-4	6.490379310747679E-5	0.0	4.1953811864673	0.0012656239655957973
88-89	1.2980758621495357E-4	6.490379310747679E-5	0.0	4.656457732702815	0.0012980758621495357
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1485	0.0	55.347473	1
GTATCAA	2635	0.0	42.911575	1
TATCAAC	4115	0.0	31.057076	1
TCAACGC	4415	0.0	28.811987	3
ATCAACG	4420	0.0	28.564621	2
CAACGCA	4560	0.0	27.999905	4
AACGCAG	4975	0.0	25.979967	5
TATACCG	210	3.4338882E-8	22.627861	5
ACGCAGA	6380	0.0	22.4887	1
TTATATG	715	0.0	21.9067	2
CGCAGAG	6535	0.0	21.49885	2
TAAGGTG	695	0.0	20.511585	5
TATGTAA	965	0.0	19.674414	2
ATTATAT	945	0.0	19.606989	1
ATATGTA	1005	0.0	18.909151	1
GTACATG	5775	0.0	18.839163	1
AGAGTAC	6025	0.0	18.770798	5
TATATGT	790	0.0	18.625319	3
CAGAGTA	6310	0.0	18.598932	9
ATGTAAG	1080	0.0	18.458426	3
>>END_MODULE