FastQCFastQC Report
Mon 15 Aug 2016
SRR2921353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512223
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.057.147931
GTATCAA47750.047.412141
TCAACGC62300.035.9575274
CAACGCA64100.034.7983325
TATCAAC64700.034.6984522
ATCAACG65700.034.0237963
AACGCAG67350.033.1776966
ACGCAGA79450.027.352927
GTACATG100850.026.9193571
CGCAGAG83750.025.6672958
TACATGG104300.025.5288812
ACATGGG106600.024.976583
CATGGGG85600.022.3997694
GCAGAGT93300.021.8193789
ATGGGGG57250.020.3099675
AGAGTAC81650.019.93446710-11
GAGTACA80100.018.8425271
CAGAGTA88350.018.36903210-11
GTGTAAG11600.018.0027661
AGTACAT80500.017.9238362
Produced by FastQC (version 0.11.5)