##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921353_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2512223
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51579059661503	31.0	31.0	34.0	27.0	34.0
2	30.582554972229776	33.0	31.0	34.0	27.0	34.0
3	30.624025812995104	33.0	31.0	34.0	26.0	34.0
4	34.08918197150492	37.0	35.0	37.0	30.0	37.0
5	34.090826331898086	37.0	35.0	37.0	30.0	37.0
6	34.12858213621959	37.0	35.0	37.0	30.0	37.0
7	34.05128286780274	37.0	35.0	37.0	30.0	37.0
8	34.021522372814836	37.0	35.0	37.0	30.0	37.0
9	35.59241436767357	39.0	37.0	39.0	30.0	39.0
10-11	35.48649343629128	39.0	37.0	39.0	30.0	39.0
12-13	35.37262277273952	39.0	37.0	39.0	30.0	39.0
14-15	36.51325539173871	40.0	37.0	41.0	29.5	41.0
16-17	36.45727130911547	40.0	37.0	41.0	29.0	41.0
18-19	36.41817426239629	40.0	37.0	41.0	29.0	41.0
20-21	36.36222660169897	40.0	37.0	41.0	28.0	41.0
22-23	36.251088975779616	40.0	37.0	41.0	27.5	41.0
24-25	36.14684564228574	40.0	36.5	41.0	27.0	41.0
26-27	35.99765267653389	40.0	36.0	41.0	27.0	41.0
28-29	35.8598366864725	40.0	36.0	41.0	27.0	41.0
30-31	35.71156422021453	40.0	36.0	41.0	26.5	41.0
32-33	35.53522676927964	40.0	35.5	41.0	25.5	41.0
34-35	35.37403188331609	39.0	35.0	41.0	25.0	41.0
36-37	35.20701446487831	39.0	35.0	41.0	25.0	41.0
38-39	35.43504418198543	39.5	35.0	41.0	25.5	41.0
40-41	35.554379925667426	40.0	35.0	41.0	25.5	41.0
42-43	35.39376301387257	40.0	35.0	41.0	24.5	41.0
44-45	35.27050305645638	40.0	35.0	41.0	24.0	41.0
46-47	35.1127320305562	40.0	35.0	41.0	23.5	41.0
48-49	34.96409096644685	40.0	35.0	41.0	23.0	41.0
50-51	33.25799958841233	37.0	32.5	39.5	19.0	40.0
52-53	33.42046904275615	37.5	33.5	39.5	20.0	40.0
54-55	34.27818967504079	39.0	34.0	40.5	20.5	41.0
56-57	34.28925875608972	39.0	34.0	41.0	20.0	41.0
58-59	34.10922278794518	38.5	34.0	41.0	20.0	41.0
60-61	33.863606654345574	38.0	34.0	40.5	18.5	41.0
62-63	33.57196892950984	37.0	34.0	40.0	17.5	41.0
64-65	33.28110900186807	37.0	34.0	40.0	15.5	41.0
66-67	32.943970141185716	36.0	33.0	39.5	13.0	41.0
68-69	32.580082659859414	36.0	33.0	39.0	9.5	41.0
70-71	32.19949502890468	35.0	33.0	39.0	7.5	40.5
72-73	31.842822671395012	35.0	32.5	37.5	4.5	40.0
74-75	31.447450723920607	35.0	32.0	37.0	2.0	39.0
76-77	31.07917310684601	35.0	32.0	36.5	2.0	39.0
78-79	30.742055343016922	35.0	32.0	36.0	2.0	38.5
80-81	30.400228005236798	35.0	31.5	36.0	2.0	37.0
82-83	30.105096959943445	35.0	31.0	35.0	2.0	37.0
84-85	29.87756739748024	35.0	31.0	35.0	2.0	36.5
86-87	29.662614743993664	35.0	31.0	35.0	2.0	36.0
88-89	29.472083887457444	35.0	31.0	35.0	2.0	36.0
90-91	29.226314304104374	35.0	31.0	35.0	2.0	35.5
92-93	29.0435518662157	35.0	31.0	35.0	2.0	35.0
94-95	28.842794807626554	34.0	30.0	35.0	2.0	35.0
96-97	28.6030999238523	34.0	30.0	35.0	2.0	35.0
98-99	28.39086677416774	34.0	30.0	35.0	2.0	35.0
100-101	27.364692147154134	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83796.0
3	14485.0
4	9861.0
5	7362.0
6	6840.0
7	6803.0
8	7184.0
9	7581.0
10	8264.0
11	9133.0
12	9115.0
13	9317.0
14	9976.0
15	10746.0
16	11092.0
17	11859.0
18	11077.0
19	9842.0
20	9956.0
21	10216.0
22	11316.0
23	13127.0
24	15447.0
25	18218.0
26	21439.0
27	25363.0
28	29970.0
29	35482.0
30	42571.0
31	52077.0
32	65356.0
33	85217.0
34	123586.0
35	180343.0
36	297018.0
37	546749.0
38	579299.0
39	105131.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60295701825895	18.50804625806886	13.6906058558137	24.198390867858496
2	16.183966220527196	19.631769654709874	39.45387144078118	24.73039268398175
3	19.015009145507353	24.35436859759728	29.263592517768327	27.36702973912704
4	13.465542510314945	16.073670208529144	36.472647475825596	33.98813980533032
5	14.485608572772312	37.55721068705391	33.755151600107205	14.202029140066577
6	33.19887619738446	34.816342991501415	17.601835156772218	14.382945654341917
7	29.001957087395336	32.008689850216726	20.895650138381523	18.093702924006408
8	27.236096037608597	34.03597309621995	19.98550024166264	18.742430624508813
9	27.55575692017242	15.448943784634702	19.314454338825783	37.680844956367096
10-11	25.97815207550505	25.293005686129778	27.404322764505363	21.32451947385981
12-13	26.47257548266983	22.779217097606587	27.335530336601764	23.412677083121817
14-15	23.889286774539805	24.169791180716878	24.821434295805116	27.119487748938205
16-17	23.90352571121419	27.093169556648284	25.60345205152197	23.399852680615556
18-19	23.86084529584957	26.618936044416753	26.897416653892936	22.622802005840747
20-21	24.517516521743286	25.590352341093748	26.874605955651237	23.017525181511733
22-23	24.40039137634013	25.40973455063003	26.4460973470992	23.743776725930633
24-25	24.162373340575378	25.679698096017063	26.340541130316154	23.81738743309141
26-27	23.77073445857528	25.93156371945811	26.449722365950564	23.84797945601604
28-29	23.788106205682936	26.326993745346833	26.245735011326854	23.639165037643377
30-31	24.439989235308822	25.77207995808777	26.13958826648662	23.648342540116786
32-33	24.115317980745036	25.836552578527822	26.025786074389245	24.022343366337896
34-35	24.426499818669445	26.063729629174038	26.075911220878133	23.433859331278388
36-37	24.390421087618947	25.885613519704957	26.140531051654236	23.583434341021857
38-39	24.224668030491394	26.054466207520306	26.031118770185184	23.68974699180312
40-41	24.428711889910158	25.75238940659918	26.098649353337606	23.720249350153058
42-43	24.12419243705272	25.824205300225454	25.91707940167301	24.13452286104882
44-45	24.04431561888903	25.8245127969554	26.17446319140723	23.95670839274834
46-47	24.274755400490385	25.779130366469076	25.825209925190006	24.120904307850534
48-49	23.94248277083152	25.98984474008899	25.93479354906116	24.13287894001833
50-51	24.151842583419054	25.87043865223148	26.100628241122248	23.877090523227213
52-53	24.3477289902436	25.71942725770014	26.028968110751975	23.903875641304285
54-55	23.706468891767212	25.604195511941146	26.44795966872332	24.24137592756832
56-57	23.828230626451226	25.743610216634057	26.113780140243016	24.314379016671705
58-59	24.079248994202906	25.71787670730268	26.236986852054507	23.96588744643991
60-61	24.233513470397032	25.773027299738764	26.31902207509921	23.67443715476499
62-63	24.154667690834515	25.61847394603537	26.40396841112203	23.822889952008087
64-65	23.807703474691593	25.48215513514462	26.380958989992777	24.329182400171014
66-67	23.867588871333467	25.67135532023669	26.19990556760331	24.26115024082654
68-69	23.93996455648551	25.592546334180337	26.304985699770146	24.162503409564007
70-71	23.90776307085889	25.477414444181733	26.244606846538794	24.370215638420582
72-73	23.804461641889958	25.879751948469337	26.033089487237355	24.28269692240335
74-75	23.80617626107187	25.88319241398051	26.144489172413394	24.166142152534224
76-77	23.762608571176795	25.400581438699927	26.19771706958404	24.639092920539234
78-79	23.751992474613086	25.463511465665622	26.319091481431922	24.46540457828937
80-81	24.011848957502345	25.746493097880542	25.953448985583087	24.288208959034023
82-83	23.955359092663187	25.699932292762504	26.182219221875318	24.162489392698987
84-85	23.934670832466676	25.7778319320257	26.060812251233077	24.22668498427455
86-87	23.67788255371024	25.99842191196774	26.015355642243136	24.308339892078884
88-89	23.587398438846304	25.654223763235663	26.166656827015743	24.59172097090229
90-91	23.8424931438544	25.459845425081028	26.275103465469957	24.422557965594617
92-93	24.01426614160947	25.33197541302578	26.316008879139307	24.33774956622544
94-95	23.872532053999333	25.33779504391061	26.30162426877983	24.48804863331023
96-97	23.626931408359926	25.18005731911932	26.444592814623295	24.748418457897454
98-99	23.365953233506946	25.192500095741423	26.380012861264294	25.061533809487337
100-101	23.64627819636614	25.178949266638647	26.458020356220903	24.716752180774318
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	47.5
2	59.5
3	81.0
4	76.5
5	88.0
6	161.0
7	341.0
8	517.5
9	591.0
10	562.0
11	478.0
12	467.5
13	536.5
14	631.5
15	697.5
16	775.0
17	896.5
18	995.5
19	1087.5
20	1159.0
21	1268.0
22	1448.0
23	1625.0
24	1880.5
25	2315.5
26	3044.5
27	4132.5
28	5756.0
29	8027.5
30	11272.5
31	15709.5
32	21849.0
33	27124.0
34	31644.5
35	37444.0
36	44741.5
37	52312.5
38	58338.0
39	64583.5
40	69873.5
41	74069.5
42	78010.0
43	82472.5
44	87368.0
45	92958.0
46	97267.5
47	100859.0
48	105512.5
49	108008.5
50	108305.5
51	107591.5
52	106592.0
53	106344.5
54	105465.5
55	101161.5
56	94302.5
57	85374.5
58	75996.5
59	66211.0
60	55186.5
61	45703.0
62	37766.5
63	29099.0
64	21107.5
65	15583.5
66	11755.5
67	9046.0
68	6996.5
69	5406.5
70	4191.5
71	3094.5
72	2145.5
73	1573.5
74	1245.5
75	975.5
76	738.5
77	517.5
78	339.5
79	233.0
80	169.5
81	127.5
82	111.5
83	92.5
84	72.5
85	63.0
86	51.5
87	37.0
88	35.0
89	36.5
90	27.5
91	30.0
92	27.0
93	23.0
94	21.0
95	15.0
96	13.0
97	11.5
98	9.5
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04697035255230129
2	0.05369746236699529
3	0.0459354125808099
4	0.05186641472512591
5	0.04418397570597833
6	0.015683321106446362
7	0.013016360410680102
8	0.018191060268137025
9	0.018788141020920517
10-11	0.019783275608893
12-13	0.024679337781717627
14-15	0.04109905848326363
16-17	0.026789023108219293
18-19	0.022211404003545862
20-21	0.024221575871250286
22-23	0.04318884111800585
24-25	0.03825297356166232
26-27	0.029197248811112707
28-29	0.019624054074817404
30-31	0.012220252740302116
32-33	0.009831929729168151
34-35	0.009394070510460258
36-37	0.0015126045737181772
38-39	0.0067669151982128984
40-41	0.003701900667257644
42-43	0.20163417021498492
44-45	0.1766960974403944
46-47	0.18324408302925338
48-49	0.18139313269562454
50-51	0.17942276621143902
52-53	0.1846173687606554
54-55	0.18274651573526715
56-57	0.19349396928536997
58-59	0.18943382016644222
60-61	0.19397163388759675
62-63	0.19192165663637345
64-65	0.1924590293138786
66-67	0.18346301263860731
68-69	0.18195040806488913
70-71	0.16646611387603727
72-73	0.1734320559918447
74-75	0.17301409946489624
76-77	0.1714019814323808
78-79	0.19809149108180285
80-81	0.2056744166421532
82-83	0.20322638555574085
84-85	0.19829051799939734
86-87	0.21463062793390555
88-89	0.21331705027778186
90-91	0.21287919105907396
92-93	0.21687963210272337
94-95	0.22730864258467498
96-97	0.2280649448715341
98-99	0.2177752532318986
100-101	0.22754747488578841
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2512223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.81568214541334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87410063377558	47.179081330152655
2	11.756452324431647	14.064404307918185
3	3.844231287591402	6.898359503760612
4	1.708628486171776	4.088111137337989
5	0.9660183528051826	2.889152336901528
6	0.5994729403237232	2.15147297119081
7	0.42205040932620536	1.7671663193518525
8	0.29760538187952995	1.4241175141816256
9	0.22890817232669017	1.2323068628742722
>10	1.2223634759690551	12.893793724177108
>50	0.05807614472439764	2.3563336497910137
>100	0.020089469220193737	2.0466133871833847
>500	0.0014020450182107584	0.589406141029397
>1k	6.008764363704702E-4	0.4196808141496546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9805383518899394E-5
2	0.0	0.0	0.0	0.0	3.9805383518899394E-5
3	0.0	0.0	0.0	0.0	3.9805383518899394E-5
4	0.0	0.0	0.0	0.0	7.961076703779879E-5
5	0.0	7.961076703779879E-5	0.0	0.0	7.961076703779879E-5
6	0.0	7.961076703779879E-5	0.0	0.0	1.194161505566982E-4
7	0.0	7.961076703779879E-5	0.0	0.0	1.194161505566982E-4
8	0.0	7.961076703779879E-5	0.0	0.0	1.194161505566982E-4
9	0.0	7.961076703779879E-5	0.0	0.0	1.194161505566982E-4
10-11	0.0	7.961076703779879E-5	0.0	0.0	1.194161505566982E-4
12-13	0.0	7.961076703779879E-5	0.0	0.0	1.3931884231614789E-4
14-15	0.0	7.961076703779879E-5	0.0	0.0	1.791242258350473E-4
16-17	0.0	7.961076703779879E-5	0.0	0.0	1.9902691759449698E-4
18-19	0.0	1.194161505566982E-4	0.0	0.0	2.7863768463229577E-4
20-21	0.0	1.194161505566982E-4	0.0	1.9902691759449697E-5	3.582484516700946E-4
22-23	0.0	1.194161505566982E-4	0.0	1.194161505566982E-4	4.577619104673431E-4
24-25	0.0	1.194161505566982E-4	0.0	2.5873499287284605E-4	5.174699857456922E-4
26-27	0.0	1.194161505566982E-4	0.0	7.164969033401892E-4	5.174699857456922E-4
28-29	1.9902691759449697E-5	1.194161505566982E-4	0.0	0.0018708530253882718	5.174699857456922E-4
30-31	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.006448472130061702	5.174699857456922E-4
32-33	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.018111449501099226	5.572753692645915E-4
34-35	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.03441175405208853	5.572753692645915E-4
36-37	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.06030515603113259	5.572753692645915E-4
38-39	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.10504640710637551	5.572753692645915E-4
40-41	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.1553803145660238	5.572753692645915E-4
42-43	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.20949573345996753	5.572753692645915E-4
44-45	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.2804488295824057	5.572753692645915E-4
46-47	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.3633435407605137	5.771780610240413E-4
48-49	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.4541794259506421	6.766915198212897E-4
50-51	3.9805383518899394E-5	1.194161505566982E-4	0.0	0.5468264560908804	7.563022868590885E-4
52-53	3.9805383518899394E-5	1.3931884231614789E-4	0.0	0.6445486726297784	8.359130538968874E-4
54-55	3.9805383518899394E-5	1.9902691759449698E-4	0.0	0.7642434608711091	9.553292044535856E-4
56-57	3.9805383518899394E-5	1.9902691759449698E-4	0.0	0.9000395267458343	9.553292044535856E-4
58-59	3.9805383518899394E-5	2.388323011133964E-4	0.0	1.0465034354036247	9.553292044535856E-4
60-61	3.9805383518899394E-5	2.388323011133964E-4	0.0	1.2039138245291123	9.553292044535856E-4
62-63	3.9805383518899394E-5	2.388323011133964E-4	0.0	1.3688275284479126	9.951345879724849E-4
64-65	3.9805383518899394E-5	3.1844306815119515E-4	0.0	1.5499022180753859	9.951345879724849E-4
66-67	3.9805383518899394E-5	3.582484516700946E-4	0.0	1.7595372703776695	9.951345879724849E-4
68-69	3.9805383518899394E-5	3.582484516700946E-4	0.0	1.9929162339489768	9.951345879724849E-4
70-71	3.9805383518899394E-5	3.582484516700946E-4	0.0	2.243471220508689	0.0010349399714913844
72-73	3.9805383518899394E-5	3.582484516700946E-4	0.0	2.501191176101803	0.0010747453550102838
74-75	3.9805383518899394E-5	3.582484516700946E-4	0.0	2.7825953348886623	0.0010946480467697334
76-77	3.9805383518899394E-5	3.582484516700946E-4	0.0	3.1072878482523247	0.0011543561220480826
78-79	3.9805383518899394E-5	3.582484516700946E-4	0.0	3.4639838899651823	0.001194161505566982
80-81	3.9805383518899394E-5	3.9805383518899396E-4	0.0	3.8344724970673383	0.0012140641973264316
82-83	3.9805383518899394E-5	3.9805383518899396E-4	0.0	4.2202861768242705	0.0012936749643642303
84-85	3.9805383518899394E-5	3.9805383518899396E-4	0.0	4.636152921137972	0.0013135776561236801
86-87	5.970807527834909E-5	3.9805383518899396E-4	0.0	5.0831673780552125	0.0013135776561236801
88-89	7.961076703779879E-5	3.9805383518899396E-4	0.0	5.577609949435221	0.0013135776561236801
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2450	0.0	57.14793	1
GTATCAA	4775	0.0	47.41214	1
TCAACGC	6230	0.0	35.957527	4
CAACGCA	6410	0.0	34.798332	5
TATCAAC	6470	0.0	34.698452	2
ATCAACG	6570	0.0	34.023796	3
AACGCAG	6735	0.0	33.177696	6
ACGCAGA	7945	0.0	27.35292	7
GTACATG	10085	0.0	26.919357	1
CGCAGAG	8375	0.0	25.667295	8
TACATGG	10430	0.0	25.528881	2
ACATGGG	10660	0.0	24.97658	3
CATGGGG	8560	0.0	22.399769	4
GCAGAGT	9330	0.0	21.819378	9
ATGGGGG	5725	0.0	20.309967	5
AGAGTAC	8165	0.0	19.934467	10-11
GAGTACA	8010	0.0	18.842527	1
CAGAGTA	8835	0.0	18.369032	10-11
GTGTAAG	1160	0.0	18.002766	1
AGTACAT	8050	0.0	17.923836	2
>>END_MODULE