FastQCFastQC Report
Mon 15 Aug 2016
SRR2921353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512223
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.061.3039551
GTATCAA46600.044.1641121
TCAACGC62850.032.2466354
CAACGCA64400.031.3724565
TATCAAC65650.031.1604942
ATCAACG65550.030.845993
AACGCAG69100.029.3050446
GTACATG99300.026.6129511
TACATGG102600.025.3047332
ACATGGG106200.023.7318613
ACGCAGA84350.023.442857
CGCAGAG85050.022.74698
CATGGGG86000.021.4138664
ATGGGGG53850.020.7456765
GCAGAGT94250.019.265679
AGAGTAC82550.018.94440510-11
CAGAGTA87150.017.39904610-11
ATACCGT2951.2223773E-616.1134596
TCTATAC6650.015.7022853
GAGTACA76450.015.6671711
Produced by FastQC (version 0.11.5)