FastQCFastQC Report
Mon 15 Aug 2016
SRR2921352_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921352_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345871
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.050.7712941
GTATCAA34300.042.4788861
TCAACGC40150.035.5871474
CAACGCA41050.034.921815
ATCAACG41650.034.3003733
AACGCAG43800.032.722256
TATCAAC44500.032.423582
GTACATG70400.027.235641
ACGCAGA52350.027.0181887
TACATGG72350.026.1747022
ACATGGG72550.025.4483453
CGCAGAG55100.025.2422588
CATGGGG57900.022.5458354
GCAGAGT67400.019.860599
GTGTAGC12600.019.5867181
GTATAGG6200.019.1371461
ATGGGGG38400.018.665895
CAGAGTA60150.017.95367610-11
GAGTACA53950.017.8580251
AGAGTAC55900.017.36557210-11
Produced by FastQC (version 0.11.5)