##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921352_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345871
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.913274003557742	33.0	31.0	34.0	28.0	34.0
2	30.981166057298122	33.0	31.0	34.0	28.0	34.0
3	31.03491624219746	34.0	31.0	34.0	27.0	34.0
4	34.46187663345512	37.0	35.0	37.0	32.0	37.0
5	34.464991041706895	37.0	35.0	37.0	32.0	37.0
6	34.512985155620235	37.0	35.0	37.0	32.0	37.0
7	34.444672788912946	37.0	35.0	37.0	32.0	37.0
8	34.41966288853905	37.0	35.0	37.0	32.0	37.0
9	36.054945902822446	39.0	37.0	39.0	32.0	39.0
10-11	35.96322197597395	39.0	37.0	39.0	31.0	39.0
12-13	35.86579014788111	39.0	37.0	39.0	31.0	39.0
14-15	37.10015448419799	40.0	38.0	41.0	31.0	41.0
16-17	37.05311864974672	40.0	38.0	41.0	31.0	41.0
18-19	37.008508566754095	40.0	38.0	41.0	31.0	41.0
20-21	36.95638229894142	40.0	38.0	41.0	31.0	41.0
22-23	36.84970443813833	40.0	38.0	41.0	30.0	41.0
24-25	36.7599407640062	40.0	38.0	41.0	30.0	41.0
26-27	36.63215283363834	40.0	37.0	41.0	30.0	41.0
28-29	36.50494016934435	40.0	37.0	41.0	30.0	41.0
30-31	36.37240773256501	40.0	37.0	41.0	29.5	41.0
32-33	36.20788824278914	40.0	37.0	41.0	29.0	41.0
34-35	36.0503947147989	40.0	36.0	41.0	28.0	41.0
36-37	35.89818003632766	40.0	36.0	41.0	27.5	41.0
38-39	36.12817030433472	40.0	37.0	41.0	28.5	41.0
40-41	36.25156391805005	40.0	37.0	41.0	29.0	41.0
42-43	36.11223549803037	40.0	37.0	41.0	28.0	41.0
44-45	36.0128928658055	40.0	36.5	41.0	28.0	41.0
46-47	35.86842903978949	40.0	36.0	41.0	27.0	41.0
48-49	35.734725183098305	40.0	36.0	41.0	27.0	41.0
50-51	34.0639640883919	38.0	33.5	39.5	24.0	40.0
52-53	34.23705395565229	38.5	34.5	39.5	23.5	40.0
54-55	35.090202956599065	39.0	35.0	40.5	25.0	41.0
56-57	35.10613946802702	39.0	35.0	41.0	25.0	41.0
58-59	34.9410619765537	39.0	35.0	41.0	25.0	41.0
60-61	34.71322250882508	39.0	35.0	41.0	24.5	41.0
62-63	34.436710501131564	38.0	35.0	41.0	23.5	41.0
64-65	34.163829980420914	37.5	34.5	40.0	23.0	41.0
66-67	33.83156618586444	37.0	34.0	40.0	22.5	41.0
68-69	33.476094380296274	36.5	34.0	39.5	22.0	41.0
70-71	33.108015103984826	36.0	34.0	39.0	21.0	41.0
72-73	32.75117600243151	35.5	34.0	39.0	20.0	40.5
74-75	32.35691284814894	35.0	33.0	37.5	18.5	39.5
76-77	31.983430887717187	35.0	33.0	37.0	17.5	39.0
78-79	31.637749049287024	35.0	33.0	36.5	14.0	39.0
80-81	31.287445260203995	35.0	33.0	36.0	9.0	38.0
82-83	30.988258305763615	35.0	33.0	36.0	7.0	37.0
84-85	30.75069110790832	35.0	32.0	35.0	2.0	37.0
86-87	30.52871556023328	35.0	32.0	35.0	2.0	36.0
88-89	30.33659054568644	35.0	32.0	35.0	2.0	36.0
90-91	30.100417286372526	35.0	32.0	35.0	2.0	36.0
92-93	29.926627252734697	35.0	32.0	35.0	2.0	35.5
94-95	29.744444174466544	35.0	32.0	35.0	2.0	35.0
96-97	29.519784335967323	35.0	31.5	35.0	2.0	35.0
98-99	29.32553814766456	35.0	31.0	35.0	2.0	35.0
100-101	28.333278343097298	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70001.0
3	11809.0
4	7865.0
5	5912.0
6	5338.0
7	5292.0
8	5518.0
9	6191.0
10	6222.0
11	6928.0
12	7032.0
13	7071.0
14	7587.0
15	8530.0
16	8504.0
17	8972.0
18	8197.0
19	7556.0
20	7623.0
21	7873.0
22	8672.0
23	9931.0
24	11705.0
25	13837.0
26	16386.0
27	19553.0
28	23035.0
29	27761.0
30	33271.0
31	40519.0
32	51328.0
33	67839.0
34	99742.0
35	151100.0
36	264709.0
37	524427.0
38	635085.0
39	136925.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.848028220187054	18.12026330198705	12.974650616548997	25.057057861276906
2	15.843363936909553	19.10740422308881	40.13456873522277	24.914663104778867
3	19.333150817186198	23.519295112405214	29.225653923798657	27.921900146609936
4	13.174135345015452	15.64752688566412	36.79048906171303	34.3878487076074
5	14.220554565508758	37.19878021686076	34.411794932808306	14.168870284822177
6	33.30786661522162	35.13149395982597	17.379628188757838	14.181011236194575
7	28.90107416495995	31.548799557987685	21.129280075851494	18.42084620120087
8	26.52236900783706	34.96782379359477	19.887939656929245	18.62186754163892
9	26.737099260074054	15.632539687615246	19.610147921236578	38.02021313107412
10-11	25.634772442923403	25.196480103211876	27.819817507519296	21.34892994634543
12-13	25.985339030131605	22.551399306987133	27.771507850862815	23.691753812018447
14-15	23.278605963792177	24.175997796178965	25.02929615039202	27.51610008963684
16-17	23.457398897597674	27.279841984025722	25.87490259909842	23.387856519278184
18-19	23.423066969156523	26.689066321938515	27.111228976606778	22.776637732298184
20-21	23.897473833890643	25.922649154018423	27.129226843299676	23.050650168791257
22-23	23.749351280147614	25.7076430179407	26.746938063618682	23.796067638293
24-25	23.50366220978589	25.87803806276106	26.77130468395704	23.846995043496
26-27	23.28223907100359	26.13192548909163	26.8308516759795	23.754983763925278
28-29	23.330360176871544	26.438083235021626	26.647042697056534	23.584513891050303
30-31	23.81403683119006	25.969546726660013	26.63860980174101	23.57780664040892
32-33	23.51015342796764	26.103644132719943	26.504618527570695	23.88158391174172
34-35	23.800271692501894	26.270742238932943	26.53431338171901	23.39467268684615
36-37	23.740466362027906	26.100070401265903	26.604389006319064	23.55507423038713
38-39	23.60194072939776	26.257590432942436	26.562529308748374	23.57793952891143
40-41	23.742680784697892	26.01640936578204	26.602522087932257	23.63838776158781
42-43	23.491081011618466	25.990839796337458	26.52117187079741	23.99690732124667
44-45	23.504791824286613	26.120665829346816	26.62337787063942	23.75116447572715
46-47	23.58837696326139	26.071880744009583	26.3958930411524	23.943849251576626
48-49	23.461819942028317	26.19540183519369	26.46022498442499	23.882553238352997
50-51	23.558725377958424	26.18610093062844	26.578487039413197	23.676686651999944
52-53	23.657970983560197	26.09684596955355	26.52930724450539	23.715875802380864
54-55	23.325161070833175	25.960347559131513	26.810847659337245	23.903643710698066
56-57	23.414467444929716	26.104463361578116	26.52613932019987	23.954929873292297
58-59	23.464405585823066	26.101076290134095	26.67970096529938	23.754817158743457
60-61	23.66128374005536	26.158799181034702	26.708283741762962	23.471633337146976
62-63	23.538023803831837	26.01505669205287	26.79344113930535	23.653478364809946
64-65	23.30300259962471	25.888539516366066	26.798995156147175	24.009462727862047
66-67	23.362112866824862	26.04370630191532	26.625273143043504	23.968907688216312
68-69	23.407746016243642	26.011001830792747	26.755633774860726	23.82561837810288
70-71	23.33545671345572	25.802440286602966	26.789737351750187	24.072365648191127
72-73	23.24381000877356	26.101267503103614	26.615537717239523	24.039384770883306
74-75	23.253309544592152	26.115940235056094	26.71553175732923	23.915218463022523
76-77	23.250297496589674	25.787875114602343	26.729172250952242	24.232655137855737
78-79	23.16283505725695	25.849760894850725	26.81892494363104	24.168479104261287
80-81	23.35462911629581	26.07247255162361	26.526090447339527	24.046807884741046
82-83	23.38901990033927	26.012163963101237	26.66826484744454	23.93055128911496
84-85	23.356652253236085	26.063135479345647	26.579875856795805	24.000336410622463
86-87	23.18386126984005	26.204535954292897	26.592637282919227	24.018965492947824
88-89	23.11384307176065	25.92895901297591	26.727654013830925	24.229543901432514
90-91	23.300991393011895	25.845920460132398	26.7706854742212	24.082402672634508
92-93	23.327700665138764	25.7741431708257	26.86750704795169	24.030649116083847
94-95	23.270433000010033	25.719764591888744	26.822607565676606	24.18719484242462
96-97	23.147095987903025	25.570098853717084	26.974266603978318	24.308538554401572
98-99	23.006382258286965	25.528371309342894	26.875020549018537	24.5902258833516
100-101	23.161071640181703	25.541675785607907	26.919669689706893	24.377582884503493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	26.5
2	32.5
3	50.0
4	58.5
5	66.0
6	104.5
7	210.5
8	339.0
9	411.0
10	391.0
11	356.5
12	369.0
13	433.0
14	482.5
15	514.5
16	587.5
17	658.5
18	737.5
19	822.5
20	913.5
21	1009.5
22	1155.0
23	1358.0
24	1655.5
25	2142.5
26	3036.5
27	4494.0
28	6657.5
29	9806.5
30	13882.5
31	19043.0
32	25725.0
33	31351.5
34	36293.5
35	41981.0
36	48821.5
37	55576.0
38	60675.0
39	65701.0
40	69601.5
41	73161.0
42	77086.0
43	81149.0
44	84982.5
45	88654.5
46	91749.0
47	94529.5
48	97374.0
49	98734.0
50	98318.0
51	96888.5
52	94972.0
53	92689.0
54	89709.5
55	85304.5
56	79117.0
57	71242.0
58	63625.5
59	55529.5
60	46402.0
61	38617.0
62	32051.0
63	25015.5
64	18462.0
65	13746.5
66	10571.0
67	8372.5
68	6572.0
69	5204.5
70	4177.0
71	3286.5
72	2469.0
73	1918.5
74	1624.5
75	1349.0
76	1037.5
77	704.5
78	458.5
79	315.5
80	229.5
81	182.0
82	148.0
83	102.5
84	69.5
85	69.5
86	63.0
87	41.5
88	36.0
89	38.0
90	32.5
91	26.5
92	25.5
93	25.0
94	18.0
95	15.0
96	14.0
97	8.0
98	4.0
99	3.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0376406034261901
2	0.04463161017805327
3	0.037214322526686244
4	0.044077445008698264
5	0.03683066971713278
6	0.013896757323825564
7	0.010017601138340514
8	0.014877203392684422
9	0.015601880921840972
10-11	0.0172430623849308
12-13	0.019608921377177176
14-15	0.03636176072767854
16-17	0.022955226438282413
18-19	0.019097384297772555
20-21	0.02210266463927471
22-23	0.03700118207693432
24-25	0.032844943306771776
26-27	0.026877010713717845
28-29	0.01875635957816947
30-31	0.011829294961231883
32-33	0.008951898889580884
34-35	0.008717444394853767
36-37	0.0013854129233875179
38-39	0.0066073539423097015
40-41	0.0032610488812044656
42-43	0.15196914067312312
44-45	0.13123057491226076
46-47	0.13696405301058753
48-49	0.1353654996374481
50-51	0.13440636761356445
52-53	0.13783792885457044
54-55	0.1360049209867039
56-57	0.14632091875469708
58-59	0.14210073784960894
60-61	0.1450633901011607
62-63	0.1445518530217561
64-65	0.1456814974054413
66-67	0.13785924289954563
68-69	0.13513104514272098
70-71	0.12398379962069525
72-73	0.12944019513434457
74-75	0.12899260018986553
76-77	0.12733010468180048
78-79	0.1488359760617698
80-81	0.1577878749513507
82-83	0.15450551202517104
84-85	0.14900648842157133
86-87	0.16318032833007443
88-89	0.16200805585643882
90-91	0.1603881884383242
92-93	0.16533304687256886
94-95	0.17692788733907364
96-97	0.17596875531518996
98-99	0.166675831706006
100-101	0.17620320980991708
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2345871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.06955750403341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68037217986684	52.49836117004663
2	11.36886955571294	14.79534622622636
3	3.384972814455947	6.607760495994933
4	1.5013571521654625	3.9077058218768967
5	0.821269417445891	2.6719818792399717
6	0.5160617233925431	2.0147944791553005
7	0.34222012012321834	1.5587678249776484
8	0.2546304636192349	1.3254953275800399
9	0.1800121497819868	1.05419798384973
>10	0.8996341627866657	10.06368730305727
>50	0.03798147673318291	1.6557433258369387
>100	0.011634872673014152	1.354677471527021
>500	8.527230773223281E-4	0.3982210024826403
>1k	1.3118816574184667E-4	0.09325968814837125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.2628089950385166E-5	0.0
3	0.0	0.0	0.0	4.2628089950385166E-5	0.0
4	0.0	0.0	0.0	4.2628089950385166E-5	0.0
5	0.0	0.0	0.0	4.2628089950385166E-5	0.0
6	0.0	0.0	0.0	4.2628089950385166E-5	0.0
7	0.0	0.0	0.0	4.2628089950385166E-5	0.0
8	0.0	0.0	0.0	4.2628089950385166E-5	4.2628089950385166E-5
9	0.0	0.0	0.0	8.525617990077033E-5	8.525617990077033E-5
10-11	0.0	0.0	0.0	1.278842698511555E-4	8.525617990077033E-5
12-13	0.0	0.0	0.0	1.278842698511555E-4	1.9182640477673327E-4
14-15	0.0	0.0	0.0	1.278842698511555E-4	4.262808995038517E-4
16-17	0.0	0.0	0.0	1.278842698511555E-4	5.11537079404622E-4
18-19	0.0	0.0	0.0	1.491983148263481E-4	5.541651693550071E-4
20-21	0.0	0.0	0.0	1.7051235980154066E-4	6.607353942309701E-4
22-23	0.0	0.0	0.0	1.7051235980154066E-4	9.165039339332811E-4
24-25	0.0	0.0	0.0	1.7051235980154066E-4	0.001023074158809244
26-27	0.0	2.1314044975192583E-5	0.0	2.1314044975192584E-4	0.0010657022487596292
28-29	0.0	4.2628089950385166E-5	0.0	7.033634841813552E-4	0.0011083303387100143
30-31	0.0	8.525617990077033E-5	0.0	0.0037725859606090872	0.0011296443836852068
32-33	0.0	8.525617990077033E-5	0.0	0.011275129791876875	0.0013427848334371328
34-35	0.0	8.525617990077033E-5	0.0	0.020994334300564695	0.0014067269683627104
36-37	0.0	8.525617990077033E-5	0.0	0.03768323151614049	0.0014067269683627104
38-39	0.0	1.278842698511555E-4	0.0	0.06711792762688144	0.0014067269683627104
40-41	0.0	1.278842698511555E-4	0.0	0.10275501082540345	0.0014919831482634808
42-43	0.0	1.278842698511555E-4	0.0	0.1413760603204524	0.0015132971932386734
44-45	0.0	1.278842698511555E-4	0.0	0.18737176937691802	0.0015559252831890587
46-47	0.0	1.278842698511555E-4	0.0	0.2463690458682511	0.0016198674181146363
48-49	0.0	1.278842698511555E-4	0.0	0.31259178360617446	0.0016624955080650214
50-51	0.0	1.278842698511555E-4	0.0	0.3813935207860961	0.0017051235980154067
52-53	0.0	1.278842698511555E-4	0.0	0.4583798512364917	0.0019608921377177175
54-55	0.0	1.278842698511555E-4	0.0	0.5516287980029593	0.0021740325874696435
56-57	0.0	1.278842698511555E-4	0.0	0.6630159970433156	0.0021740325874696435
58-59	0.0	1.278842698511555E-4	0.0	0.7893869697012326	0.002195346632444836
60-61	0.0	1.278842698511555E-4	0.0	0.921811131132104	0.0022592887673704137
62-63	0.0	1.491983148263481E-4	0.0	1.0680254796619253	0.0025150573070727247
64-65	0.0	1.7051235980154066E-4	0.0	1.2308434692274213	0.0025150573070727247
66-67	0.0	1.7051235980154066E-4	0.0	1.4202613869219578	0.0025789994419983028
68-69	0.0	1.7051235980154066E-4	0.0	1.6284143501496886	0.0026429415769238804
70-71	0.0	1.7051235980154066E-4	0.0	1.8575829617229593	0.0026855696668742655
72-73	0.0	1.7051235980154066E-4	0.0	2.0957887283657115	0.0029413382065765765
74-75	0.0	1.7051235980154066E-4	0.0	2.346186128734274	0.0030265943864773467
76-77	0.0	1.7051235980154066E-4	0.0	2.638785338153718	0.0030265943864773467
78-79	0.0	1.9182640477673327E-4	0.0	2.9676823661659144	0.0030479084314525393
80-81	0.0	2.55768539702311E-4	0.0	3.316273571735189	0.003069222476427732
82-83	0.0	2.55768539702311E-4	0.0	3.680743740810982	0.0031971067462788876
84-85	0.0	2.55768539702311E-4	0.0	4.065760649242861	0.0032397348362292726
86-87	0.0	2.55768539702311E-4	0.0	4.489249408854962	0.0032823629261796577
88-89	0.0	2.55768539702311E-4	0.0	4.955685969092077	0.003324991016130043
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	50.771294	1
GTATCAA	3430	0.0	42.478886	1
TCAACGC	4015	0.0	35.587147	4
CAACGCA	4105	0.0	34.92181	5
ATCAACG	4165	0.0	34.300373	3
AACGCAG	4380	0.0	32.72225	6
TATCAAC	4450	0.0	32.42358	2
GTACATG	7040	0.0	27.23564	1
ACGCAGA	5235	0.0	27.018188	7
TACATGG	7235	0.0	26.174702	2
ACATGGG	7255	0.0	25.448345	3
CGCAGAG	5510	0.0	25.242258	8
CATGGGG	5790	0.0	22.545835	4
GCAGAGT	6740	0.0	19.86059	9
GTGTAGC	1260	0.0	19.586718	1
GTATAGG	620	0.0	19.137146	1
ATGGGGG	3840	0.0	18.66589	5
CAGAGTA	6015	0.0	17.953676	10-11
GAGTACA	5395	0.0	17.858025	1
AGAGTAC	5590	0.0	17.365572	10-11
>>END_MODULE