FastQCFastQC Report
Mon 15 Aug 2016
SRR2921352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345871
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.056.7256931
GTATCAA35900.042.6255571
TCAACGC51150.029.5076224
ATCAACG52100.028.9695783
CAACGCA53250.028.4707095
TATCAAC53600.028.424512
AACGCAG56300.026.9260356
GTACATG67050.022.0429781
ACGCAGA69150.021.5788147
TACATGG68700.021.2096942
CGCAGAG70350.021.0080818
TAACGCT2201.3561294E-619.4165734
ACATGGG74000.019.3699263
AGAGTAC70700.018.5515310-11
GCAGAGT79450.018.242999
GTACCGT2905.192851E-818.0253966
CATGGGG59800.017.5405754
CAGAGTA74550.017.0197729
TAAGGTG10100.016.469255
TACACTG32700.016.4232035
Produced by FastQC (version 0.11.5)