##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345871
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.12050918400884	31.0	31.0	34.0	26.0	34.0
2	30.29150110982232	31.0	31.0	34.0	26.0	34.0
3	30.350902926887283	33.0	31.0	34.0	26.0	34.0
4	33.83748125962595	37.0	35.0	37.0	30.0	37.0
5	33.485429931995405	37.0	35.0	37.0	28.0	37.0
6	33.41808778061539	37.0	35.0	37.0	28.0	37.0
7	33.23708592671976	37.0	35.0	37.0	26.0	37.0
8	33.17857247904936	37.0	35.0	37.0	26.0	37.0
9	34.66341158571805	39.0	35.0	39.0	27.0	39.0
10-11	34.465386417241184	39.0	35.0	39.0	26.0	39.0
12-13	34.36317129117501	39.0	35.0	39.0	25.0	39.0
14-15	35.44308169545555	40.0	36.0	41.0	25.0	41.0
16-17	35.39317144889894	40.0	36.0	41.0	25.0	41.0
18-19	35.336783011512566	40.0	36.0	41.0	25.0	41.0
20-21	35.28818677582868	40.0	36.0	41.0	24.0	41.0
22-23	35.160325951427	40.0	36.0	41.0	23.0	41.0
24-25	35.07450026024449	40.0	36.0	41.0	23.0	41.0
26-27	34.91060207487965	39.5	35.0	41.0	21.5	41.0
28-29	34.738842630306614	39.0	35.0	41.0	19.0	41.0
30-31	34.5789129069757	39.0	35.0	41.0	18.0	41.0
32-33	34.518532135825026	39.0	35.0	41.0	18.0	41.0
34-35	34.6436511214811	39.0	35.0	41.0	18.0	41.0
36-37	34.60882226686804	39.0	35.0	41.0	17.0	41.0
38-39	34.51124486384801	39.0	35.0	41.0	16.0	41.0
40-41	34.37669420014997	39.0	35.0	41.0	15.0	41.0
42-43	34.25392828505915	39.0	35.0	41.0	14.0	41.0
44-45	34.10277461974678	39.0	34.0	41.0	9.0	41.0
46-47	33.93450598945978	39.0	34.0	41.0	8.5	41.0
48-49	33.77074719794908	39.0	34.0	41.0	7.0	41.0
50-51	33.613863038504675	39.0	34.0	40.5	2.0	41.0
52-53	33.436790641940675	38.5	33.5	40.0	2.0	41.0
54-55	33.224885980516405	38.0	33.0	40.0	2.0	41.0
56-57	32.969511324365236	38.0	33.0	40.0	2.0	41.0
58-59	32.74715873123458	38.0	33.0	40.0	2.0	41.0
60-61	32.487992306482326	37.0	32.5	40.0	2.0	41.0
62-63	32.20227604160672	37.0	32.0	40.0	2.0	41.0
64-65	31.90172882481603	36.0	32.0	39.5	2.0	41.0
66-67	31.588147003820758	36.0	32.0	39.0	2.0	41.0
68-69	31.244310109123646	35.0	31.0	39.0	2.0	40.0
70-71	30.88684565349075	35.0	31.0	38.0	2.0	40.0
72-73	30.51178048579824	35.0	31.0	37.0	2.0	39.5
74-75	30.112181147215683	35.0	30.5	37.0	2.0	39.0
76-77	27.522052150352685	32.0	27.0	34.5	2.0	36.5
78-79	29.086423123863163	34.0	29.5	35.5	2.0	37.0
80-81	29.294993629231957	34.0	30.0	35.5	2.0	37.0
82-83	29.132101466789948	34.0	30.0	35.0	2.0	37.0
84-85	28.895702918020643	34.0	30.0	35.0	2.0	36.0
86-87	28.632217628335063	34.0	29.5	35.0	2.0	36.0
88-89	28.34108674347396	34.0	29.0	35.0	2.0	36.0
90-91	28.13166751283425	34.0	29.0	35.0	2.0	35.0
92-93	27.96227520609616	34.0	29.0	35.0	2.0	35.0
94-95	27.8322874531464	34.0	29.0	35.0	2.0	35.0
96-97	27.61185909199611	34.0	29.0	35.0	2.0	35.0
98-99	27.303100852519172	34.0	28.5	35.0	2.0	35.0
100-101	26.200903630250767	32.5	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108954.0
3	27988.0
4	13815.0
5	9143.0
6	7535.0
7	7401.0
8	7519.0
9	7915.0
10	8622.0
11	8824.0
12	9359.0
13	10188.0
14	10800.0
15	11342.0
16	12278.0
17	12978.0
18	11907.0
19	10066.0
20	9789.0
21	9502.0
22	10280.0
23	11677.0
24	13759.0
25	16632.0
26	19975.0
27	23695.0
28	28366.0
29	33994.0
30	41119.0
31	50808.0
32	64515.0
33	84795.0
34	121831.0
35	181756.0
36	310252.0
37	529630.0
38	444819.0
39	42043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.662215675363825	23.095327909985627	14.810612396776031	27.431844017874518
2	13.347011295615353	23.429343364028465	35.49179705916706	27.73184828118912
3	15.78101500233612	26.88970094229267	29.05474880721367	28.274535248157534
4	12.503996541872247	19.114767041041393	34.03945300876724	34.34178340831912
5	13.570481416578195	38.21567599142139	34.365707756132295	13.848134835868114
6	33.27299970799435	36.93837401874378	16.388806592080893	13.399819681180983
7	28.303998090260755	32.26431984722086	21.08829560035296	18.343386462165423
8	26.58638862636822	36.42599027131778	19.077544272921465	17.91007682939254
9	26.405203014146984	15.422885572139302	19.19977696983338	38.972134443880336
10-11	25.63962173843677	26.12093773648813	27.485946153012677	20.753494372062427
12-13	25.98143884542474	23.221691596326647	27.63134056619578	23.16552899205283
14-15	23.223101671208852	24.75938778755275	24.771537228230866	27.245973313007532
16-17	23.53987446440412	27.98468882633447	25.451852906503497	23.023583802757912
18-19	23.458398154879855	27.30329364858612	26.901863642108758	22.33644455442527
20-21	23.98748515732753	26.273645701385146	26.959600435576885	22.779268705710436
22-23	23.800715002324303	26.13081349684647	26.596419702485203	23.472051798344022
24-25	23.567011482664743	26.37530130458571	26.62753499225544	23.430152220494104
26-27	23.39662061436322	26.666768976699316	26.58713766795364	23.34947274098382
28-29	23.473932027918664	26.85661363086528	26.483021900315144	23.18643244090091
30-31	23.89686587507413	26.508558485005974	26.445077032546926	23.149498607372973
32-33	23.66644696228133	26.561322250909797	26.28648570756181	23.48574507924706
34-35	23.8500715251472	26.69285240922829	26.443777354825055	23.013298710799454
36-37	23.875155220458876	26.527746576197952	26.511462548654922	23.085635654688243
38-39	23.822715212817112	26.623303823239542	26.396329881016122	23.157651082927227
40-41	23.91906541601887	26.434656238822974	26.37467595941803	23.271602385740124
42-43	23.75280649313748	26.47485704017583	26.244219951046105	23.528116515640583
44-45	23.755661172271655	26.49028954550115	26.242558970300255	23.51149031192694
46-47	23.85822865972521	26.56680015407148	26.07855897550566	23.496412210697653
48-49	23.61119874489474	26.659262796190347	26.23131624048226	23.498222218432655
50-51	23.75373307235132	26.600129467883587	26.21449677939581	23.431640680369288
52-53	24.00010486677763	26.41952210163253	26.179287660441375	23.401085371148465
54-55	23.514741326612825	26.43235683039098	26.48920180028254	23.563700042713656
56-57	23.69789318666787	26.447486972697178	26.155852731742325	23.698767108892625
58-59	23.6494992682252	26.488563807224132	26.354636016109705	23.507300908440968
60-61	23.79911062757839	26.616245306836046	26.34845732551363	23.236186740071936
62-63	23.771469964007462	26.459249810060687	26.378861353254717	23.390418872677134
64-65	23.517526206639676	26.26399185215334	26.44353187917496	23.774950062032026
66-67	23.565148052125267	26.537426656205227	26.24678054854336	23.65064474312615
68-69	23.577181867661793	26.433147294708164	26.283123939502023	23.70654689812802
70-71	23.541512615362574	26.28492646902044	26.324734605317364	23.848826310299625
72-73	23.438453691435416	26.55430851341657	26.16943689100989	23.83780090413812
74-75	23.401384531552328	26.547770005397936	26.113206517429017	23.937638945620723
76-77	23.394373548347968	26.200124887989485	26.366464243338523	24.039037320324024
78-79	23.29562223734874	26.289031112293937	26.39638106546428	24.018965584893042
80-81	23.53499121543831	26.45857949617836	26.098816563126338	23.907612725256996
82-83	23.545716351133876	26.391128770079785	26.228684828695187	23.834470050091145
84-85	23.404360560934727	26.494409542729976	26.12387997903765	23.977349917297648
86-87	23.30621375607567	26.588312531514134	26.110406768063626	23.995066944346565
88-89	23.229828738034648	26.40627590249638	26.14220929815237	24.221686061316603
90-91	23.191870290196256	26.327475663809963	26.273724138623795	24.206929907369986
92-93	23.37703680644102	26.213192492867716	26.271572770653783	24.13819793003748
94-95	23.25601718831263	26.27106975078652	26.244383531277443	24.228529529623408
96-97	23.02519012977147	26.208354391364114	26.34594655200014	24.42050892686428
98-99	22.88755457235501	26.12759066156518	26.215319925135987	24.76953484094382
100-101	22.846610546104436	26.180956308874105	26.27853319223264	24.69389995278882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	25.0
2	35.5
3	46.5
4	39.5
5	44.5
6	81.0
7	210.0
8	321.0
9	323.5
10	286.0
11	258.5
12	261.5
13	273.0
14	316.5
15	375.0
16	419.0
17	463.5
18	566.0
19	647.5
20	702.0
21	854.5
22	1032.0
23	1273.0
24	1692.5
25	2311.5
26	3316.0
27	4963.5
28	7340.0
29	10709.0
30	15115.0
31	20501.0
32	27089.0
33	32581.0
34	37418.0
35	43133.0
36	49901.5
37	56734.5
38	62309.5
39	67047.0
40	70670.0
41	74338.0
42	78007.5
43	81953.5
44	85990.0
45	89748.0
46	92918.0
47	95488.5
48	98016.5
49	99153.5
50	98152.0
51	96222.0
52	94552.0
53	92179.0
54	88713.5
55	83756.5
56	77410.5
57	69704.5
58	61817.5
59	53638.5
60	44807.0
61	37381.5
62	30957.0
63	23873.0
64	17416.0
65	13239.5
66	10225.0
67	7987.0
68	6259.5
69	4866.5
70	3811.0
71	2935.5
72	2210.5
73	1681.5
74	1324.5
75	1080.5
76	835.5
77	557.5
78	349.5
79	217.0
80	142.0
81	97.5
82	56.0
83	36.5
84	21.0
85	12.5
86	7.5
87	6.5
88	7.0
89	4.5
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10328786194978325
2	2.1314044975192584E-4
3	0.004390693264889672
4	0.00396441236538582
5	0.021356673065142967
6	0.0011083303387100143
7	4.2628089950385166E-5
8	0.001023074158809244
9	0.0
10-11	0.07131679448699438
12-13	1.7051235980154066E-4
14-15	0.0035807595558323543
16-17	1.0657022487596292E-4
18-19	3.197106746278887E-4
20-21	7.033634841813553E-4
22-23	1.9182640477673324E-4
24-25	0.0013001567434867477
26-27	0.0025576853970231098
28-29	0.005477709558624494
30-31	0.012958939344917092
32-33	0.012575286535363625
34-35	0.008205907315449144
36-37	5.967932593053923E-4
38-39	2.983966296526962E-4
40-41	0.004262808995038517
42-43	0.008291163495349915
44-45	0.007566485966193366
46-47	0.008802700574754537
48-49	0.010699650577546677
50-51	0.004923544389269487
52-53	0.0015985533731394438
54-55	7.246775291565479E-4
56-57	0.005307197198822953
58-59	0.008696130349878574
60-61	0.007332031471466249
62-63	0.01641181463089829
64-65	0.030329885999699047
66-67	0.03201369555273926
68-69	0.024276697226744352
70-71	0.037214322526686244
72-73	0.02316836688803434
74-75	0.022550259583753753
76-77	0.19493825534311135
78-79	0.05128159221031336
80-81	0.0482763118688112
82-83	0.019033442162846977
84-85	0.030436456224575005
86-87	0.071828331566399
88-89	0.22861444640391565
90-91	0.27292634590734105
92-93	0.038407909045297034
94-95	0.00387915618548505
96-97	0.0072680893365406705
98-99	7.459915741317404E-4
100-101	0.0012149005635859772
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2345871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.4854030689188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.40763683001046	55.613125874303336
2	10.371492529235965	13.998487075235385
3	3.130220570689034	6.337325907227115
4	1.3387838215374355	3.6139346327440514
5	0.7466007837011849	2.5192327409822566
6	0.46270871954055687	1.8735650665018675
7	0.30822014302927964	1.456025241040352
8	0.21257229628055957	1.1476421676623354
9	0.17122982370529077	1.0399962303154313
>10	0.8082036114197182	9.347921144231805
>50	0.031498038962204794	1.4220570173013487
>100	0.010069793751670036	1.2233698670028064
>500	6.358651139388092E-4	0.3111091979590838
>1k	1.2717302278746786E-4	0.09620783749283977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.2628089950385166E-5
2	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
3	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
4	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
5	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
6	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
7	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	4.2628089950385166E-5
8	0.0	4.2628089950385166E-5	0.0	4.2628089950385166E-5	8.525617990077033E-5
9	0.0	4.2628089950385166E-5	0.0	8.525617990077033E-5	8.525617990077033E-5
10-11	0.0	4.2628089950385166E-5	0.0	8.525617990077033E-5	8.525617990077033E-5
12-13	0.0	4.2628089950385166E-5	0.0	8.525617990077033E-5	1.278842698511555E-4
14-15	0.0	4.2628089950385166E-5	0.0	8.525617990077033E-5	2.1314044975192584E-4
16-17	0.0	4.2628089950385166E-5	0.0	1.0657022487596292E-4	2.55768539702311E-4
18-19	2.1314044975192583E-5	4.2628089950385166E-5	0.0	1.491983148263481E-4	2.55768539702311E-4
20-21	4.2628089950385166E-5	4.2628089950385166E-5	0.0	1.7051235980154066E-4	3.410247196030813E-4
22-23	4.2628089950385166E-5	4.2628089950385166E-5	0.0	2.1314044975192584E-4	4.262808995038517E-4
24-25	4.2628089950385166E-5	4.2628089950385166E-5	0.0	2.3445449472711842E-4	5.11537079404622E-4
26-27	4.2628089950385166E-5	4.2628089950385166E-5	0.0	3.197106746278888E-4	5.11537079404622E-4
28-29	4.2628089950385166E-5	4.2628089950385166E-5	0.0	7.459915741317404E-4	5.11537079404622E-4
30-31	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.0033463050611052354	5.11537079404622E-4
32-33	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.010124171363216477	5.11537079404622E-4
34-35	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.019161326432698134	5.541651693550071E-4
36-37	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.03465663712966314	5.541651693550071E-4
38-39	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.06170416020318252	5.754792143301997E-4
40-41	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.09437859115015276	5.967932593053923E-4
42-43	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.1299091041237988	5.967932593053923E-4
44-45	4.2628089950385166E-5	4.2628089950385166E-5	0.0	0.17228142553448167	6.181073042805849E-4
46-47	6.394213492557776E-5	4.2628089950385166E-5	0.0	0.22693063685087544	6.820494392061627E-4
48-49	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.2882511442445045	7.67305619106933E-4
50-51	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.35161779995575204	7.67305619106933E-4
52-53	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.4226148837681185	8.738758439828959E-4
54-55	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.5089580799626237	0.001023074158809244
56-57	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.6122459419124069	0.0010870162937348217
58-59	8.525617990077033E-5	4.2628089950385166E-5	0.0	0.7286206274769584	0.001172272473635592
60-61	1.278842698511555E-4	4.2628089950385166E-5	0.0	0.8505582787800352	0.0011935865186107847
62-63	1.278842698511555E-4	4.2628089950385166E-5	0.0	0.9863713733619623	0.0012362146085611698
64-65	1.278842698511555E-4	4.2628089950385166E-5	0.0	1.136955101111698	0.0012362146085611698
66-67	1.278842698511555E-4	4.2628089950385166E-5	0.0	1.313371451371367	0.0012575286535363623
68-69	1.278842698511555E-4	4.2628089950385166E-5	0.0	1.508053938174776	0.0012788426985115549
70-71	1.278842698511555E-4	4.2628089950385166E-5	0.0	1.7220469497257096	0.0014067269683627104
72-73	1.278842698511555E-4	4.2628089950385166E-5	0.0	1.9471445787087185	0.0015559252831890587
74-75	1.278842698511555E-4	4.2628089950385166E-5	0.0	2.1844338414175377	0.0016198674181146363
76-77	1.278842698511555E-4	4.2628089950385166E-5	0.0	2.460173641261604	0.0016624955080650214
78-79	1.278842698511555E-4	4.2628089950385166E-5	0.0	2.773170391722307	0.0016624955080650214
80-81	1.278842698511555E-4	4.2628089950385166E-5	0.0	3.104497220861676	0.0016624955080650214
82-83	1.278842698511555E-4	4.2628089950385166E-5	0.0	3.450061832044473	0.0018116938228913697
84-85	1.278842698511555E-4	4.2628089950385166E-5	0.0	3.8157255876388767	0.0018543219128417548
86-87	1.278842698511555E-4	4.2628089950385166E-5	0.0	4.2191152028393715	0.00189695000279214
88-89	1.278842698511555E-4	4.2628089950385166E-5	0.0	4.662340768098502	0.0019182640477673324
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1885	0.0	56.725693	1
GTATCAA	3590	0.0	42.625557	1
TCAACGC	5115	0.0	29.507622	4
ATCAACG	5210	0.0	28.969578	3
CAACGCA	5325	0.0	28.470709	5
TATCAAC	5360	0.0	28.42451	2
AACGCAG	5630	0.0	26.926035	6
GTACATG	6705	0.0	22.042978	1
ACGCAGA	6915	0.0	21.578814	7
TACATGG	6870	0.0	21.209694	2
CGCAGAG	7035	0.0	21.008081	8
TAACGCT	220	1.3561294E-6	19.416573	4
ACATGGG	7400	0.0	19.369926	3
AGAGTAC	7070	0.0	18.55153	10-11
GCAGAGT	7945	0.0	18.24299	9
GTACCGT	290	5.192851E-8	18.025396	6
CATGGGG	5980	0.0	17.540575	4
CAGAGTA	7455	0.0	17.019772	9
TAAGGTG	1010	0.0	16.46925	5
TACACTG	3270	0.0	16.423203	5
>>END_MODULE