FastQCFastQC Report
Mon 15 Aug 2016
SRR2921351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727100
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.060.769091
GTATCAA31500.056.2988741
TCAACGC40250.043.1716234
CAACGCA40650.042.7468155
GTACATG102400.040.622931
AACGCAG43200.040.082336
ATCAACG43750.039.4458583
TACATGG106600.038.40722
TATCAAC45350.038.3166052
ACATGGG107700.037.6834033
CATGGGG95500.036.1414874
ATGGGGG61650.032.9156725
ACGCAGA53250.032.2865267
CGCAGAG56800.030.1638838
TGGGGGG44050.027.4216656
GCAGAGT64250.026.2951569
ATGGGGA31900.024.251255
AGAGTAC59450.023.21489110-11
GAGTACA71450.022.9879841
AGTACAT70750.022.5418622
Produced by FastQC (version 0.11.5)