##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921351_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1727100
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.496678246772046	31.0	31.0	34.0	27.0	34.0
2	30.68729778240982	33.0	31.0	34.0	27.0	34.0
3	30.764927334838745	33.0	31.0	34.0	27.0	34.0
4	34.17612298071912	37.0	35.0	37.0	30.0	37.0
5	34.21817844942389	37.0	35.0	37.0	31.0	37.0
6	34.26340339297088	37.0	35.0	37.0	31.0	37.0
7	34.20472989404204	37.0	35.0	37.0	30.0	37.0
8	34.15109489896358	37.0	35.0	37.0	30.0	37.0
9	35.80013375021713	39.0	37.0	39.0	30.0	39.0
10-11	35.794585432227436	39.0	37.0	39.0	30.0	39.0
12-13	35.72118059174339	39.0	37.0	39.0	30.0	39.0
14-15	37.03544641306236	41.0	38.0	41.0	31.0	41.0
16-17	37.02289444733947	41.0	38.0	41.0	30.5	41.0
18-19	37.033116495860114	41.0	38.0	41.0	30.0	41.0
20-21	37.05962393607782	41.0	38.0	41.0	30.0	41.0
22-23	37.01766110821609	41.0	38.0	41.0	30.0	41.0
24-25	37.004425626773205	41.0	38.0	41.0	30.0	41.0
26-27	36.96977360893984	41.0	38.0	41.0	30.0	41.0
28-29	36.898658734294486	40.0	38.0	41.0	30.0	41.0
30-31	36.820427595391116	40.0	38.0	41.0	30.0	41.0
32-33	36.73733165421805	40.0	38.0	41.0	30.0	41.0
34-35	36.631331133113314	40.0	38.0	41.0	30.0	41.0
36-37	36.528185397487114	40.0	37.0	41.0	29.5	41.0
38-39	36.4250772972034	40.0	37.0	41.0	29.0	41.0
40-41	36.31735655144462	40.0	37.0	41.0	28.5	41.0
42-43	36.13816918533959	40.0	36.5	41.0	28.0	41.0
44-45	35.92245990388513	40.0	36.0	41.0	27.0	41.0
46-47	35.714030745179784	40.0	35.5	41.0	27.0	41.0
48-49	35.51423571304498	40.0	35.0	41.0	26.0	41.0
50-51	35.34548028487059	39.5	35.0	41.0	26.0	41.0
52-53	35.157441375716516	39.0	35.0	41.0	26.0	41.0
54-55	34.95241676799259	39.0	35.0	41.0	25.5	41.0
56-57	34.706285391697065	39.0	35.0	40.5	24.5	41.0
58-59	34.445795553239535	38.0	34.5	40.0	24.0	41.0
60-61	34.16025041977882	38.0	34.0	40.0	23.0	41.0
62-63	33.831516704302004	37.0	34.0	40.0	22.5	41.0
64-65	33.491668403682475	36.5	34.0	39.5	22.0	41.0
66-67	33.156489491054366	36.0	33.5	39.0	22.0	41.0
68-69	32.80766950379248	35.5	33.0	39.0	20.5	40.5
70-71	32.74142377395634	35.0	33.0	38.5	21.5	40.0
72-73	32.450588269353254	35.0	33.0	37.5	20.5	40.0
74-75	32.12132447455272	35.0	33.0	37.0	20.0	39.0
76-77	31.81856667245672	35.0	33.0	36.5	19.5	39.0
78-79	31.530015633142263	35.0	33.0	36.0	18.5	38.0
80-81	31.258659313299752	35.0	33.0	36.0	18.0	37.0
82-83	31.03942302124949	35.0	33.0	35.0	16.5	37.0
84-85	30.830304846274103	35.0	32.5	35.0	13.5	36.0
86-87	30.66565890799606	35.0	32.0	35.0	11.5	36.0
88-89	30.538262984193153	35.0	32.0	35.0	10.0	36.0
90-91	30.421417694401022	35.0	32.0	35.0	7.0	35.5
92-93	30.318659602802384	35.0	32.0	35.0	7.0	35.0
94-95	30.207460482890394	35.0	32.0	35.0	6.0	35.0
96-97	30.125197730299348	35.0	32.0	35.0	2.0	35.0
98-99	30.053505587400842	35.0	32.0	35.0	2.0	35.0
100-101	29.977204852064155	35.0	32.0	35.0	2.0	35.0
102-103	29.169828324937757	33.5	30.0	35.0	2.0	35.0
104-105	29.76101586474437	34.5	31.0	35.0	2.0	35.0
106-107	29.84269961206647	35.0	31.0	35.0	2.0	35.0
108-109	29.794287823519195	35.0	31.0	35.0	2.0	35.0
110-111	29.726979908517166	35.0	31.0	35.0	2.0	35.0
112-113	29.65603005037346	35.0	31.0	35.0	2.0	35.0
114-115	29.546683168316832	34.5	31.0	35.0	2.0	35.0
116-117	29.426474436917374	34.0	31.0	35.0	2.0	35.0
118-119	29.43702391291761	34.5	31.0	35.0	2.0	35.0
120-121	29.61074546928377	35.0	32.0	35.0	2.0	35.0
122-123	29.496781309709917	35.0	32.0	35.0	2.0	35.0
124-125	28.95174917491749	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58353.0
3	8971.0
4	5597.0
5	4475.0
6	4873.0
7	5425.0
8	5624.0
9	6176.0
10	6613.0
11	7446.0
12	7258.0
13	7032.0
14	7312.0
15	6566.0
16	5268.0
17	4707.0
18	4517.0
19	4509.0
20	4407.0
21	4444.0
22	5080.0
23	6867.0
24	10004.0
25	12682.0
26	13656.0
27	14489.0
28	17278.0
29	20674.0
30	25252.0
31	31776.0
32	40987.0
33	56953.0
34	90996.0
35	151363.0
36	316153.0
37	572502.0
38	170726.0
39	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.463027297297145	17.85944066513744	12.900907516268523	24.776624521296895
2	16.036435652737886	19.4818979894376	39.99281941999444	24.488846937830075
3	19.239321782636896	25.12328592606319	28.08174279625683	27.555649495043085
4	13.293325511674867	15.539407781546407	35.73631204942701	35.43095465735171
5	14.443102954077599	38.159050572256625	32.40289877731357	14.994947696352206
6	32.92668588410345	34.50798322905065	17.932741288008447	14.632589598837455
7	29.344028667077044	32.290129072495205	20.49119239034571	17.874649870082038
8	27.19188220088113	35.19554431364919	19.233388461912636	18.379185023557042
9	28.233317833937193	16.071233140063775	18.428395974635038	37.267053051363995
10-11	26.526777506749568	25.84549461844134	26.53830062899455	21.089427245814544
12-13	26.26183606908602	23.178182692015387	26.884803073577974	23.675178165320627
14-15	24.05988697653398	24.150304591263453	24.17870666620729	27.611101765995276
16-17	24.303580806861326	27.057816009491532	24.690954845157506	23.947648338489635
18-19	24.386395385027395	26.63267684334716	25.945179105233823	23.03574866639162
20-21	25.57850217978776	25.567297798556226	25.333366531499944	23.52083349015607
22-23	25.317906590673662	25.144803042962916	25.310176427228477	24.227113939134952
24-25	24.699869542483814	25.357310571691123	25.343876863854582	24.59894302197048
26-27	24.360203322093312	25.42753719064732	25.58645314584976	24.625806341409607
28-29	24.464569288606384	26.056117626111213	25.267262980982892	24.212050104299514
30-31	25.254117898429275	25.556954574788065	25.26619083285008	23.922736693932585
32-33	24.77152060758755	25.55573055789911	25.33514074724328	24.337608087270066
34-35	24.73486723818954	25.792300365091357	25.484737675633262	23.988094721085833
36-37	24.782661942801155	25.88473316500437	25.274012283324225	24.058592608870246
38-39	24.880282065297955	25.901199553439074	25.176030240142307	24.042488141120664
40-41	24.795718979728385	25.580763782146953	25.34978139477114	24.273735843353524
42-43	24.584345459546316	25.675588818598992	25.222860782582263	24.51720493927243
44-45	24.332083556507765	25.67935262525514	25.62194009756945	24.36662372066764
46-47	24.31194493359036	25.510572790027176	25.43816117938565	24.739321096996814
48-49	24.314283993667463	25.784936946786075	25.34158408388411	24.55919497566235
50-51	24.329383889000194	25.73616353038864	25.485844189383407	24.448608391227765
52-53	24.41091624570644	25.818932671840944	25.36496730754532	24.405183774907297
54-55	24.177751244726	25.356336022314014	25.720062085024985	24.745850647935
56-57	24.281038882529003	25.56241535034462	25.37098190994522	24.785563857181156
58-59	24.37106681521183	25.788345149776603	25.474819510681122	24.36576852433045
60-61	24.836965002737397	25.584038045021835	25.47940612846564	24.099590823775127
62-63	24.65808512615652	25.61810166349016	25.59716942921011	24.126643781143212
64-65	24.46301520965937	25.338334218766057	25.536536042419705	24.662114529154863
66-67	24.459497292021865	25.65221205349194	25.36990602055035	24.518384633935845
68-69	24.327124071361155	25.526436169288765	25.554085431878587	24.592354327471497
70-71	24.311288205833144	25.35997899260022	25.77398921430993	24.55474358725671
72-73	24.34613354657211	25.947740480615256	25.23975609946755	24.466369873345087
74-75	24.268828075149013	25.80276915986015	25.42414051471261	24.504262250278224
76-77	24.27164705766546	25.25137332492575	25.554005673379415	24.922973944029373
78-79	24.117982553914658	25.35588450726245	25.72669343701778	24.799439501805118
80-81	24.489494588568814	25.622271883342496	25.260988680912437	24.62724484717625
82-83	24.52646316974367	25.710884282838954	25.41586469979224	24.346787847625137
84-85	24.537526958834952	25.792088137475655	25.120118637693235	24.550266265996154
86-87	24.017751208612022	26.077820196053114	25.36837405854494	24.536054536789926
88-89	24.096242527556264	25.561316308166937	25.510448103429507	24.83199306084729
90-91	24.534758150671248	25.32497010720872	25.50122900628545	24.639042735834582
92-93	24.485228484462976	25.646115374715794	25.37728968696646	24.491366453854774
94-95	24.397089363983817	25.482020166458547	25.375533482939694	24.745356986617942
96-97	24.06014449827759	25.49803380659791	25.55521584118971	24.88660585393479
98-99	23.99968963098224	25.565431839981613	25.366876735149553	25.068001793886594
100-101	23.895036182140032	25.69450194764548	25.53479907143027	24.87566279878422
102-103	23.892814283708333	25.574022041079846	25.63956913785591	24.89359453735591
104-105	24.155824228000995	25.603325979583207	25.46053583939873	24.780313953017064
106-107	24.10021109170981	25.512391757955893	25.60860047441349	24.77879667592081
108-109	24.112318321328306	25.420436604960845	25.628573968092937	24.838671105617912
110-111	23.902829530418973	25.947254287145366	25.49753469236493	24.65238149007073
112-113	23.791362744910703	26.062256840881364	25.49873856909671	24.647641845111224
114-115	24.016037770808918	26.094700321010183	25.320894381626914	24.56836752655398
116-117	23.783717117405587	25.920124930914813	25.4928317876684	24.8033261640112
118-119	23.883688500179932	25.649697396048854	25.65970155139802	24.806912552373202
120-121	23.771260971595094	25.631354404779653	25.87247977230909	24.724904851316158
122-123	23.794574059721878	25.88663190036452	25.620601075066784	24.698192964846815
124-125	23.707336929222965	25.701066752284007	25.546816578072214	25.044779740420815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	365.0
1	322.0
2	245.5
3	193.5
4	173.5
5	145.0
6	131.0
7	165.5
8	214.0
9	244.0
10	244.5
11	260.5
12	288.0
13	310.5
14	338.5
15	385.5
16	409.0
17	435.5
18	514.0
19	581.5
20	651.0
21	762.5
22	862.5
23	1061.0
24	1281.5
25	1596.5
26	2180.0
27	2973.0
28	3893.0
29	5236.0
30	7258.5
31	9467.0
32	12474.5
33	15761.0
34	19116.0
35	22991.5
36	26922.5
37	30409.0
38	33681.5
39	37178.5
40	40222.5
41	42922.0
42	46171.5
43	50900.0
44	56391.5
45	61383.0
46	64544.5
47	67695.0
48	72676.5
49	76850.5
50	78338.0
51	78504.5
52	77650.5
53	75999.0
54	75408.0
55	73119.5
56	68601.5
57	62990.5
58	57023.0
59	50846.5
60	43110.5
61	36018.0
62	29880.5
63	23407.5
64	17656.0
65	13474.0
66	10246.5
67	7782.5
68	6019.0
69	4611.0
70	3613.5
71	2870.5
72	2209.0
73	1750.0
74	1367.0
75	1016.0
76	710.0
77	468.5
78	313.0
79	217.0
80	148.5
81	103.0
82	75.0
83	46.0
84	41.0
85	38.0
86	26.0
87	18.5
88	10.5
89	11.0
90	11.5
91	7.5
92	7.0
93	9.5
94	7.5
95	3.5
96	2.5
97	2.0
98	1.5
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017312257541543627
2	0.012738115916854843
3	0.012738115916854843
4	0.015691042788489377
5	0.009553586937641132
6	0.031439986103873545
7	0.013954026981645532
8	0.0132013201320132
9	0.008742979561114006
10-11	0.007961322448034278
12-13	0.007092814544612356
14-15	0.006571709802559203
16-17	0.005037345839847143
18-19	0.006513809275664408
20-21	0.0056163511087950905
22-23	0.005732152162584679
24-25	0.0058479532163742695
26-27	0.005008395576399745
28-29	0.005934804006716461
30-31	0.005587400845347692
32-33	0.00804817323837647
34-35	0.00781657113079729
36-37	0.007208615598401946
38-39	0.0071796653349545485
40-41	0.007440217705981124
42-43	0.007237565861849342
44-45	0.007237565861849342
46-47	0.009669387991430722
48-49	0.008453476926640032
50-51	0.0061374558508482426
52-53	0.005558450581900295
54-55	0.006571709802559203
56-57	0.007469167969428522
58-59	0.007411267442533727
60-61	0.005413699264663308
62-63	0.005703201899137283
64-65	0.0041977881998726185
66-67	0.004255688726767414
68-69	0.006369057958427422
70-71	0.005182097157084129
72-73	0.004892594522610156
74-75	0.00451624109779399
76-77	0.005037345839847143
78-79	0.003763534248161658
80-81	0.003560882404029877
82-83	0.005558450581900295
84-85	0.00935093509350935
86-87	0.006890162700480574
88-89	0.0050952463667419375
90-91	0.0055005500550055
92-93	0.00836662613629784
94-95	0.00715071507150715
96-97	0.009206183776272364
98-99	0.00715071507150715
100-101	0.008279775345955647
102-103	0.005645301372242487
104-105	0.005211047420531527
106-107	0.00726651612529674
108-109	0.006687510856348792
110-111	0.00781657113079729
112-113	0.005384749001215911
114-115	0.0030108273985293265
116-117	0.003792484511609056
118-119	0.1630768339991894
120-121	0.011435354061721963
122-123	0.002576573446818366
124-125	0.0020554687047652136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1727100.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.43703127515755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43356724757712	48.61166019314306
2	10.648873065888539	12.871725490565773
3	3.4714882629206834	6.294193341524392
4	1.6063110986449716	3.883226964257553
5	0.9503102805739263	2.8716966074075065
6	0.6030309776533734	2.1867241233795456
7	0.4160978147963067	1.7603401652458373
8	0.3047535506230417	1.4734719896176065
9	0.23585196197358302	1.2828773161894236
>10	1.2381457197613144	13.20676071215781
>50	0.06602261233207132	2.6913664265512183
>100	0.024686434713592515	2.4999219952950265
>500	7.65308925801947E-4	0.27630385566664595
>1k	9.566361572524337E-5	0.08973081899866803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.790052689479474E-5	0.0
5	0.0	0.0	0.0	5.790052689479474E-5	0.0
6	0.0	0.0	0.0	5.790052689479474E-5	0.0
7	0.0	0.0	0.0	5.790052689479474E-5	0.0
8	0.0	0.0	0.0	5.790052689479474E-5	0.0
9	0.0	0.0	0.0	2.895026344739737E-4	0.0
10-11	0.0	0.0	0.0	3.474031613687685E-4	0.0
12-13	0.0	0.0	0.0	4.053036882635632E-4	8.68507903421921E-5
14-15	0.0	0.0	0.0	4.053036882635632E-4	1.4475131723698685E-4
16-17	0.0	0.0	0.0	4.053036882635632E-4	2.3160210757917896E-4
18-19	0.0	0.0	0.0	4.053036882635632E-4	2.3160210757917896E-4
20-21	0.0	0.0	0.0	5.211047420531526E-4	2.3160210757917896E-4
22-23	0.0	0.0	0.0	5.790052689479474E-4	2.605523710265763E-4
24-25	0.0	0.0	0.0	8.974581668693186E-4	3.184528979213711E-4
26-27	0.0	0.0	0.0	0.001331712118580279	3.474031613687685E-4
28-29	0.0	0.0	0.0	0.0023739216026865844	3.474031613687685E-4
30-31	0.0	0.0	0.0	0.0061374558508482426	3.474031613687685E-4
32-33	0.0	0.0	0.0	0.01728330727809623	4.632042151583579E-4
34-35	0.0	0.0	0.0	0.03094783162526779	4.632042151583579E-4
36-37	0.0	0.0	0.0	0.05086561287707718	4.632042151583579E-4
38-39	0.0	0.0	0.0	0.09130913091309131	5.211047420531526E-4
40-41	0.0	0.0	0.0	0.1453013722424874	5.790052689479474E-4
42-43	0.0	0.0	0.0	0.19295350587690346	5.790052689479474E-4
44-45	0.0	0.0	0.0	0.24491922876498173	6.079555323953448E-4
46-47	0.0	0.0	0.0	0.30093798853569564	6.369057958427422E-4
48-49	0.0	0.0	0.0	0.36425221469515373	7.527068496323316E-4
50-51	0.0	0.0	0.0	0.42962190955937696	7.527068496323316E-4
52-53	0.0	0.0	0.0	0.5059927045336112	7.81657113079729E-4
54-55	0.0	0.0	0.0	0.5819002952926872	8.106073765271264E-4
56-57	0.0	0.0	0.0	0.6585895431648428	8.106073765271264E-4
58-59	0.0	0.0	0.0	0.7503039777661977	8.106073765271264E-4
60-61	0.0	0.0	0.0	0.8483874703259799	8.106073765271264E-4
62-63	0.0	0.0	0.0	0.9652307335996757	8.106073765271264E-4
64-65	0.0	0.0	0.0	1.0651670430200915	8.106073765271264E-4
66-67	0.0	0.0	0.0	1.1740200335823054	8.395576399745238E-4
68-69	5.790052689479474E-5	0.0	0.0	1.3038330148804356	8.685079034219212E-4
70-71	5.790052689479474E-5	0.0	0.0	1.441202014938336	8.685079034219212E-4
72-73	5.790052689479474E-5	0.0	0.0	1.571680852295756	0.0010422094841063053
74-75	5.790052689479474E-5	0.0	0.0	1.7053442186323895	0.0010422094841063053
76-77	5.790052689479474E-5	0.0	0.0	1.859678073070465	0.0011001100110011
78-79	5.790052689479474E-5	0.0	0.0	2.0347982166637717	0.0011001100110011
80-81	5.790052689479474E-5	0.0	0.0	2.2161137166348217	0.0011290602744484974
82-83	5.790052689479474E-5	0.0	0.0	2.406982803543512	0.0012159110647906896
84-85	5.790052689479474E-5	0.0	0.0	2.605552660529211	0.0012159110647906896
86-87	5.790052689479474E-5	0.0	0.0	2.817787041862081	0.0012159110647906896
88-89	5.790052689479474E-5	0.0	0.0	3.0667014069828036	0.0012159110647906896
90-91	5.790052689479474E-5	0.0	0.0	3.3317121185802794	0.0012159110647906896
92-93	5.790052689479474E-5	0.0	0.0	3.5968386312315443	0.0012738115916854844
94-95	5.790052689479474E-5	0.0	0.0	3.859967575704939	0.0013317121185802791
96-97	5.790052689479474E-5	0.0	0.0	4.157199930519368	0.0013317121185802791
98-99	5.790052689479474E-5	0.0	0.0	4.48176133402814	0.0013317121185802791
100-101	1.1580105378958948E-4	0.0	0.0	4.827890683805222	0.0013317121185802791
102-103	1.1580105378958948E-4	0.0	0.0	5.212031729488738	0.0013317121185802791
104-105	1.1580105378958948E-4	0.0	0.0	5.602686584447918	0.0013317121185802791
106-107	1.1580105378958948E-4	0.0	0.0	6.013317121185803	0.0013317121185802791
108-109	1.1580105378958948E-4	0.0	0.0	6.4453998031382085	0.0013317121185802791
110-111	1.1580105378958948E-4	0.0	0.0	6.923629205025765	0.001389612645475074
112-113	1.1580105378958948E-4	0.0	0.0	7.388715187308204	0.001389612645475074
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1655	0.0	60.76909	1
GTATCAA	3150	0.0	56.298874	1
TCAACGC	4025	0.0	43.171623	4
CAACGCA	4065	0.0	42.746815	5
GTACATG	10240	0.0	40.62293	1
AACGCAG	4320	0.0	40.08233	6
ATCAACG	4375	0.0	39.445858	3
TACATGG	10660	0.0	38.4072	2
TATCAAC	4535	0.0	38.316605	2
ACATGGG	10770	0.0	37.683403	3
CATGGGG	9550	0.0	36.141487	4
ATGGGGG	6165	0.0	32.915672	5
ACGCAGA	5325	0.0	32.286526	7
CGCAGAG	5680	0.0	30.163883	8
TGGGGGG	4405	0.0	27.421665	6
GCAGAGT	6425	0.0	26.295156	9
ATGGGGA	3190	0.0	24.25125	5
AGAGTAC	5945	0.0	23.214891	10-11
GAGTACA	7145	0.0	22.987984	1
AGTACAT	7075	0.0	22.541862	2
>>END_MODULE