FastQCFastQC Report
Mon 15 Aug 2016
SRR2921351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727100
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.12222801227491169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.10213652944241793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.10178912628104916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.070.5454561
GTATCAA42300.053.111331
GTACATG97000.038.5604671
TCAACGC59300.037.1846924
TACATGG101750.036.3140342
ACATGGG101100.036.075923
CAACGCA60950.035.98255
ATCAACG62050.035.53673
TATCAAC63300.035.399842
CATGGGG89750.035.1267854
AACGCAG63850.034.7205546
ATGGGGG58300.031.7913515
ACGCAGA78050.028.1664527
CGCAGAG80850.027.1133738
TGGGGGG44950.026.7809686
AGAGTAC80200.024.17538810-11
GCAGAGT88650.023.517579
ATGGGGA31400.023.1551385
GTAAGTA8500.023.1372762
GTATATA4250.022.494211
Produced by FastQC (version 0.11.5)