FastQCFastQC Report
Mon 15 Aug 2016
SRR2921350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535629
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.062.107551
GTATCAA44850.056.9215621
TCAACGC56000.045.1594474
CAACGCA57750.043.790115
AACGCAG61300.041.4466486
ATCAACG62000.040.9811253
TATCAAC62650.040.650922
GTACATG130800.037.39781
TACATGG133750.036.3031272
ACATGGG135700.035.2991073
ACGCAGA74350.034.0858047
CATGGGG127800.031.8473224
CGCAGAG79850.031.7380038
ATGGGGG73900.029.8722955
GTATAGG8800.027.725741
GCAGAGT90950.027.6672179
AGAGTAC81100.023.98582510-11
GTATATA7150.023.3041881
CAGAGTA87050.022.65387710-11
TGGGGGG50850.022.3493256
Produced by FastQC (version 0.11.5)