##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921350_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2535629 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.6288656581858 31.0 31.0 34.0 27.0 34.0 2 30.741989857349004 33.0 31.0 34.0 27.0 34.0 3 30.91486767188733 34.0 31.0 34.0 27.0 34.0 4 34.31715128672215 37.0 35.0 37.0 32.0 37.0 5 34.386056477505186 37.0 35.0 37.0 32.0 37.0 6 34.39772379949906 37.0 35.0 37.0 31.0 37.0 7 34.3582996566138 37.0 35.0 37.0 31.0 37.0 8 34.28536193583525 37.0 35.0 37.0 31.0 37.0 9 35.9291079254891 39.0 37.0 39.0 31.0 39.0 10-11 35.93479408856737 39.0 37.0 39.0 31.0 39.0 12-13 35.866481255735756 39.0 37.0 39.0 31.0 39.0 14-15 37.19877079809389 41.0 38.0 41.0 31.0 41.0 16-17 37.18646458137212 41.0 38.0 41.0 31.0 41.0 18-19 37.19495852902771 41.0 38.0 41.0 31.0 41.0 20-21 37.21514503896272 41.0 38.0 41.0 31.0 41.0 22-23 37.162974551876474 41.0 38.0 41.0 31.0 41.0 24-25 37.13822250810351 41.0 38.0 41.0 30.5 41.0 26-27 37.09157155877299 41.0 38.0 41.0 30.0 41.0 28-29 37.02229032717326 41.0 38.0 41.0 30.0 41.0 30-31 36.94571268115328 40.0 38.0 41.0 30.0 41.0 32-33 36.85961274303141 40.0 38.0 41.0 30.0 41.0 34-35 36.749645945838296 40.0 38.0 41.0 30.0 41.0 36-37 36.65185738923162 40.0 38.0 41.0 30.0 41.0 38-39 36.55761982529779 40.0 37.5 41.0 30.0 41.0 40-41 36.45911251212224 40.0 37.0 41.0 29.0 41.0 42-43 36.297104978685766 40.0 37.0 41.0 28.5 41.0 44-45 36.10564104606786 40.0 36.5 41.0 28.0 41.0 46-47 35.90816972830016 40.0 36.0 41.0 27.5 41.0 48-49 35.712256800975226 40.0 35.5 41.0 27.0 41.0 50-51 35.548019051683035 40.0 35.0 41.0 27.0 41.0 52-53 35.35562635543292 39.0 35.0 41.0 26.0 41.0 54-55 35.14747405870496 39.0 35.0 41.0 26.0 41.0 56-57 34.90258748421003 39.0 35.0 41.0 26.0 41.0 58-59 34.64312779984769 38.0 35.0 40.5 25.0 41.0 60-61 34.36123186791127 38.0 34.0 40.0 24.5 41.0 62-63 34.04649931042751 37.0 34.0 40.0 23.5 41.0 64-65 33.7010765376165 37.0 34.0 40.0 23.0 41.0 66-67 33.36676442018923 36.0 34.0 39.0 22.0 41.0 68-69 33.019206674162504 36.0 33.5 39.0 22.0 40.5 70-71 32.950910405268274 35.0 34.0 39.0 22.5 40.0 72-73 32.65448237892846 35.0 34.0 37.5 22.0 40.0 74-75 32.3295322383519 35.0 33.0 37.0 21.5 39.0 76-77 32.029702689155236 35.0 33.0 36.5 20.5 39.0 78-79 31.742837576001854 35.0 33.0 36.0 20.0 38.5 80-81 31.466561945773613 35.0 33.0 36.0 19.5 37.0 82-83 31.248743408440273 35.0 33.0 35.0 18.5 37.0 84-85 31.036920424872882 35.0 33.0 35.0 18.0 36.5 86-87 30.874936159824642 35.0 33.0 35.0 17.5 36.0 88-89 30.744028601976076 35.0 33.0 35.0 16.5 36.0 90-91 30.624283757600185 35.0 32.5 35.0 15.0 35.5 92-93 30.520372657040916 35.0 32.0 35.0 11.5 35.0 94-95 30.412691683207598 35.0 32.0 35.0 10.0 35.0 96-97 30.336325818958528 35.0 32.0 35.0 7.0 35.0 98-99 30.265463322907255 35.0 32.0 35.0 7.0 35.0 100-101 30.186191867974376 35.0 32.0 35.0 6.0 35.0 102-103 29.391251046584497 34.0 30.5 35.0 2.0 35.0 104-105 29.97929014851936 34.5 31.5 35.0 2.0 35.0 106-107 30.06522799668248 35.0 32.0 35.0 2.0 35.0 108-109 30.0197483543531 35.0 32.0 35.0 2.0 35.0 110-111 29.955160435536904 35.0 32.0 35.0 2.0 35.0 112-113 29.883300159447614 35.0 32.0 35.0 2.0 35.0 114-115 29.77086592715259 35.0 31.0 35.0 2.0 35.0 116-117 29.656704312815478 35.0 31.0 35.0 2.0 35.0 118-119 29.66893500587034 35.0 31.5 35.0 2.0 35.0 120-121 29.84371234908577 35.0 32.0 35.0 2.0 35.0 122-123 29.73111799872931 35.0 32.0 35.0 2.0 35.0 124-125 29.200115829247892 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85689.0 3 12564.0 4 7809.0 5 6381.0 6 7062.0 7 7540.0 8 7814.0 9 8385.0 10 9055.0 11 9503.0 12 9716.0 13 9691.0 14 9536.0 15 8798.0 16 7125.0 17 6511.0 18 6308.0 19 6141.0 20 6115.0 21 6473.0 22 7384.0 23 9555.0 24 13244.0 25 16852.0 26 18479.0 27 20648.0 28 24793.0 29 29433.0 30 35472.0 31 44620.0 32 57772.0 33 78871.0 34 124408.0 35 213940.0 36 464846.0 37 863356.0 38 273619.0 39 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02963262160186 17.900235052436138 12.934589628582438 25.135542697379552 2 16.085000341178333 19.70176675156025 39.43780910658214 24.77542380067928 3 19.01167574236533 24.670123399525586 28.41754682405766 27.90065403405142 4 13.205531841378054 15.634764677436785 35.79584315142355 35.363860329761614 5 14.558980772742567 37.65468399403489 33.10248318714402 14.683852046078524 6 33.08880761263339 34.878941029390816 17.442370107135012 14.589881250840783 7 29.48311375015876 31.647982177750745 20.843283011038718 18.025621061051776 8 27.108749758408372 34.730659850824566 19.74156598705463 18.419024403712424 9 27.777201490109 15.731062540797552 18.64312520583659 37.848610763256865 10-11 26.265843129598625 25.505155980997117 26.92928743139656 21.299713458007695 12-13 26.244473100433634 22.930808275721716 27.182830754655846 23.641887869188803 14-15 23.87897877147148 24.072099851072153 24.240531091843046 27.808390285613317 16-17 24.166380728137536 27.06857791923518 24.813469479513 23.951571873114286 18-19 24.27341558274064 26.521272874063435 26.166386441271584 23.038925101924345 20-21 25.375426866820703 25.368662862916914 25.602602741373875 23.653307528888508 22-23 25.15544871636858 25.01161021620145 25.499643558967573 24.333297508462394 24-25 24.57542422081569 25.417535669887105 25.53317488814706 24.473865221150145 26-27 24.358964246189913 25.545532134414195 25.61023322366423 24.48527039573166 28-29 24.452591090203285 25.819163527041855 25.440673169740887 24.287572213013974 30-31 24.83365605271892 25.606427489499538 25.367656556370193 24.192259901411347 32-33 24.49590450450273 25.58294869759595 25.43950035645158 24.481646441449747 34-35 24.597279853057557 25.62066281469953 25.653694888942226 24.12836244330069 36-37 24.613606128504383 25.73785285420901 25.457861010613186 24.19068000667342 38-39 24.758714926376523 25.704075573634423 25.375473119104434 24.16173638088462 40-41 24.626971485501766 25.597635430210147 25.44233629844569 24.3330567858424 42-43 24.415898355783156 25.580275866203472 25.459921622824037 24.543904155189335 44-45 24.417700715028616 25.59014807391114 25.51984422323218 24.472306987828066 46-47 24.49168068502957 25.530643516811192 25.475740179421898 24.501935618737342 48-49 24.31217474320132 25.745183058850067 25.44278507478404 24.499857123164563 50-51 24.405170628777643 25.709975448387386 25.446829489543383 24.43802443329159 52-53 24.536459311121664 25.651946531707555 25.460148831544586 24.35144532562619 54-55 24.227649555297397 25.380912367526427 25.686379800734265 24.705058276441914 56-57 24.373626217595696 25.537946862599476 25.345336308243148 24.743090611561684 58-59 24.465225007695917 25.644568753573104 25.452747664060077 24.4374585746709 60-61 24.742702492497504 25.519140489270516 25.460907146510987 24.277249871720997 62-63 24.5254789285253 25.539548270493427 25.59825516101011 24.33671763997117 64-65 24.370255806125222 25.283396965569573 25.666334126526035 24.68001310177917 66-67 24.444679767731973 25.696967760841073 25.438322489399606 24.420029982027348 68-69 24.45031540702375 25.516416389333035 25.470487731191337 24.562780472451877 70-71 24.400754643607982 25.303869865586748 25.677937875406503 24.617437615398767 72-73 24.47208383791594 25.7162509418711 25.321340486919503 24.49032473329346 74-75 24.332963718960823 25.67856249128632 25.473535959311 24.514937830441855 76-77 24.265620248731317 25.293304258280493 25.62978528554644 24.811290207441747 78-79 24.275948835407018 25.34708055573906 25.627493918954226 24.749476689899698 80-81 24.47175002425517 25.61937447693629 25.257617503476578 24.651257995331967 82-83 24.43101593226382 25.65480594632031 25.423015881780366 24.491162239635507 84-85 24.454486829312543 25.593908569377582 25.33181612903862 24.61978847227125 86-87 24.176345792582666 25.81176277559823 25.395542941478773 24.616348490340332 88-89 24.317836987640707 25.445148710254895 25.474156784004425 24.762857518099974 90-91 24.45580538672998 25.265195287695867 25.503137460609192 24.775861864964956 92-93 24.43475499969433 25.59320897450309 25.49225863323611 24.479777392566472 94-95 24.414209055274245 25.371853676650534 25.554070605904815 24.659866662170405 96-97 24.243536388131655 25.46905940423209 25.55332730001757 24.734076907618682 98-99 24.157089769752176 25.403302461661514 25.478318967659284 24.96128880092702 100-101 24.141728975971272 25.445170934397993 25.60731481490611 24.805785274724624 102-103 24.115031944553454 25.397468020936415 25.659784491105654 24.827715543404484 104-105 24.16450338355621 25.40502891345211 25.598679233823102 24.83178846916858 106-107 24.137245575299772 25.31387230390475 25.671129821970744 24.877752298824735 108-109 24.13034955613297 25.2988036809332 25.726105565731288 24.84474119720254 110-111 23.982760218741312 25.70488460468996 25.47906358525133 24.833291591317398 112-113 24.007976378497776 25.6995649552857 25.59986022406351 24.692598442153017 114-115 24.069855927375457 25.729803832405562 25.49930261995223 24.701037620266753 116-117 23.907547274362567 25.57198266486745 25.673637358760615 24.846832702009372 118-119 23.958028422164944 25.427784196960268 25.73918367750027 24.875003703374514 120-121 23.8737439973179 25.52481437291077 25.8151716249396 24.786270004831728 122-123 23.92829859625307 25.67015476987235 25.668951884999476 24.732594748875105 124-125 23.906557098068866 25.510114942120747 25.67615187133676 24.907176088473626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 355.0 1 310.0 2 239.5 3 185.0 4 147.0 5 136.5 6 143.5 7 179.0 8 222.0 9 252.0 10 252.5 11 255.5 12 296.0 13 368.0 14 414.0 15 445.0 16 508.5 17 565.5 18 613.5 19 668.5 20 745.5 21 859.0 22 988.0 23 1250.5 24 1555.0 25 1978.0 26 2674.5 27 3654.0 28 4991.0 29 6892.5 30 9819.0 31 13338.0 32 17790.0 33 22786.5 34 27945.5 35 33401.0 36 39543.0 37 45591.5 38 50648.5 39 56336.0 40 61226.0 41 65198.0 42 70041.5 43 78092.5 44 86433.5 45 93068.0 46 97458.0 47 101167.0 48 108312.5 49 113375.5 50 112833.5 51 111525.5 52 110942.5 53 109316.5 54 108951.5 55 106530.0 56 99268.0 57 90386.0 58 82550.5 59 74849.0 60 63720.0 61 52805.5 62 43927.0 63 34099.5 64 25462.5 65 19659.0 66 15110.0 67 11361.0 68 8883.0 69 6917.5 70 5384.0 71 4209.5 72 3274.5 73 2757.5 74 2131.5 75 1566.0 76 1166.0 77 759.0 78 492.5 79 326.0 80 219.5 81 152.5 82 122.5 83 91.5 84 68.5 85 50.0 86 34.5 87 29.0 88 30.0 89 23.5 90 17.0 91 13.0 92 11.0 93 11.5 94 13.5 95 11.0 96 5.5 97 4.5 98 5.0 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017747075774886625 2 0.011752507957591588 3 0.011318690549761026 4 0.013527215535080251 5 0.00859747226427841 6 0.03115597747146763 7 0.014631478027739864 8 0.014158222673742886 9 0.009622858864605193 10-11 0.008834099941276898 12-13 0.007434052852369175 14-15 0.006822764686789747 16-17 0.004693115593803352 18-19 0.006704450848290504 20-21 0.005856535005712586 22-23 0.005777659113379757 24-25 0.006191757548127111 26-27 0.0048508673784690105 28-29 0.006369228305875978 30-31 0.005698783221046927 32-33 0.008735505075860862 34-35 0.008459439452695958 36-37 0.0078087133409501155 38-39 0.0076706805293676635 40-41 0.007867870260199738 42-43 0.007769275394783701 44-45 0.008124216910281434 46-47 0.010411617787933487 48-49 0.009011570699025764 50-51 0.006073443709627868 52-53 0.005718502194130135 54-55 0.00672416982137371 56-57 0.007966465125615774 58-59 0.0076312425832012485 60-61 0.005521312463298062 62-63 0.00597484884421183 64-65 0.003766323858892606 66-67 0.00433817407830562 68-69 0.006901640579122577 70-71 0.0050283381362178775 72-73 0.00463395867455373 74-75 0.004081827428223924 76-77 0.004811429432302596 78-79 0.0034113823433948734 80-81 0.0034311013164780805 82-83 0.005718502194130135 84-85 0.010411617787933487 86-87 0.007493209771618797 88-89 0.005048057109301085 90-91 0.005481874517131647 92-93 0.008834099941276898 94-95 0.007473490798535591 96-97 0.010352460868683865 98-99 0.007374895933119553 100-101 0.008932694806692934 102-103 0.005600188355630891 104-105 0.005166370947800329 106-107 0.00800590307178219 108-109 0.007296020040786724 110-111 0.008439720479612752 112-113 0.005619907328714098 114-115 0.002662061366232994 116-117 0.0033325064510620443 118-119 0.16382522837528676 120-121 0.011989135634590076 122-123 0.002287400877652054 124-125 0.001912740389071114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2535629.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.822776864837685 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22943863707947 43.66984497299199 2 11.340827523557795 12.661529686203533 3 3.9969301547492466 6.693592205187252 4 1.8624141294504262 4.158605135129283 5 1.1181764003241386 3.1209855845410908 6 0.6774887848641824 2.2691583159541966 7 0.48526380859269724 1.8962141315356011 8 0.36800533982715067 1.643446397619184 9 0.26876091093994997 1.3502682325252207 >10 1.5322676401481923 15.298653560913177 >50 0.084233485143063 3.182448357961058 >100 0.03449685032452632 3.3676162110842913 >500 0.0014842931241486588 0.5442545129707621 >1k 2.1204187487579822E-4 0.14338269538327078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.943794616641472E-5 7 0.0 0.0 0.0 0.0 3.943794616641472E-5 8 0.0 0.0 0.0 0.0 7.887589233282945E-5 9 0.0 0.0 0.0 0.0 7.887589233282945E-5 10-11 0.0 0.0 0.0 0.0 7.887589233282945E-5 12-13 0.0 0.0 0.0 0.0 9.859486541603682E-5 14-15 0.0 0.0 0.0 0.0 2.563466500816957E-4 16-17 0.0 0.0 0.0 0.0 4.33817407830562E-4 18-19 0.0 0.0 0.0 0.0 4.33817407830562E-4 20-21 0.0 0.0 0.0 0.0 4.33817407830562E-4 22-23 3.943794616641472E-5 0.0 0.0 5.915691924962209E-5 4.535363809137693E-4 24-25 3.943794616641472E-5 0.0 0.0 3.549415154977325E-4 5.126933001633914E-4 26-27 3.943794616641472E-5 0.0 0.0 5.718502194130135E-4 5.126933001633914E-4 28-29 3.943794616641472E-5 0.0 0.0 0.0018141455236550773 5.126933001633914E-4 30-31 3.943794616641472E-5 0.0 0.0 0.006586137009791259 5.915691924962209E-4 32-33 3.943794616641472E-5 0.0 0.0 0.017825951667219456 6.310071386626356E-4 34-35 3.943794616641472E-5 0.0 0.0 0.03306871786053875 6.704450848290503E-4 36-37 3.943794616641472E-5 0.0 0.0 0.05659345274880513 6.704450848290503E-4 38-39 3.943794616641472E-5 0.0 0.0 0.09885121206611851 6.901640579122577E-4 40-41 3.943794616641472E-5 0.0 0.0 0.14767538942013994 7.09883030995465E-4 42-43 3.943794616641472E-5 3.943794616641472E-5 0.0 0.19596715450091476 7.493209771618798E-4 44-45 3.943794616641472E-5 3.943794616641472E-5 0.0 0.2503126443182343 7.887589233282944E-4 46-47 3.943794616641472E-5 3.943794616641472E-5 0.0 0.30712300577095464 7.887589233282944E-4 48-49 3.943794616641472E-5 3.943794616641472E-5 0.0 0.3664771147514088 8.281968694947092E-4 50-51 3.943794616641472E-5 3.943794616641472E-5 0.0 0.435532958488801 8.67634815661124E-4 52-53 7.887589233282945E-5 3.943794616641472E-5 0.0 0.5037211674105321 9.267917349107461E-4 54-55 7.887589233282945E-5 3.943794616641472E-5 0.0 0.577982820041891 9.859486541603682E-4 56-57 7.887589233282945E-5 3.943794616641472E-5 0.0 0.6572530918363846 9.859486541603682E-4 58-59 7.887589233282945E-5 3.943794616641472E-5 0.0 0.7467575106610629 9.859486541603682E-4 60-61 7.887589233282945E-5 3.943794616641472E-5 0.0 0.8431832890379467 9.859486541603682E-4 62-63 7.887589233282945E-5 3.943794616641472E-5 0.0 0.9454853213936266 0.0010648245464931975 64-65 7.887589233282945E-5 3.943794616641472E-5 0.0 1.0436266504287497 0.0010648245464931975 66-67 7.887589233282945E-5 3.943794616641472E-5 0.0 1.1514302762746442 0.0010648245464931975 68-69 7.887589233282945E-5 3.943794616641472E-5 0.0 1.2730963401980337 0.0010648245464931975 70-71 7.887589233282945E-5 3.943794616641472E-5 0.0 1.4075797366255078 0.0010845435195764048 72-73 7.887589233282945E-5 3.943794616641472E-5 0.0 1.5416293156451517 0.0012817332504084784 74-75 7.887589233282945E-5 3.943794616641472E-5 0.0 1.678814211385025 0.0013408901696581007 76-77 7.887589233282945E-5 3.943794616641472E-5 0.0 1.8329179860302907 0.0013408901696581007 78-79 7.887589233282945E-5 3.943794616641472E-5 0.0 2.0058139420238525 0.0014000470889077225 80-81 7.887589233282945E-5 3.943794616641472E-5 0.0 2.189634209105512 0.0014394850350741375 82-83 7.887589233282945E-5 3.943794616641472E-5 0.0 2.384556258033017 0.0015775178466565889 84-85 9.859486541603682E-5 3.943794616641472E-5 0.0 2.583619291307995 0.0015775178466565889 86-87 1.1831383849924418E-4 3.943794616641472E-5 0.0 2.795420781194725 0.0015775178466565889 88-89 1.1831383849924418E-4 3.943794616641472E-5 0.0 3.034375297017032 0.0015775178466565889 90-91 1.1831383849924418E-4 3.943794616641472E-5 0.0 3.296164383669693 0.0015775178466565889 92-93 1.1831383849924418E-4 3.943794616641472E-5 0.0 3.5685819968142027 0.0015972368197397962 94-95 1.1831383849924418E-4 3.943794616641472E-5 0.0 3.850464717038652 0.0016761127120726257 96-97 1.1831383849924418E-4 3.943794616641472E-5 0.0 4.148477557245165 0.0016958316851558332 98-99 1.1831383849924418E-4 3.943794616641472E-5 0.0 4.470646139478607 0.0017155506582390407 100-101 1.1831383849924418E-4 3.943794616641472E-5 0.0 4.823674914587268 0.001735269631322248 102-103 1.1831383849924418E-4 3.943794616641472E-5 0.0 5.198177651383542 0.0018141455236550773 104-105 1.3803281158245153E-4 5.915691924962209E-5 0.0 5.588909103027296 0.001853583469821492 106-107 1.577517846656589E-4 7.887589233282945E-5 0.0 6.001745523497325 0.001912740389071114 108-109 1.577517846656589E-4 7.887589233282945E-5 0.0 6.432407895634574 0.0019521783352375289 110-111 1.577517846656589E-4 7.887589233282945E-5 0.0 6.895665730278365 0.0020310542275703584 112-113 1.577517846656589E-4 7.887589233282945E-5 0.0 7.38538642679982 0.0020507732006535655 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2290 0.0 62.10755 1 GTATCAA 4485 0.0 56.921562 1 TCAACGC 5600 0.0 45.159447 4 CAACGCA 5775 0.0 43.79011 5 AACGCAG 6130 0.0 41.446648 6 ATCAACG 6200 0.0 40.981125 3 TATCAAC 6265 0.0 40.65092 2 GTACATG 13080 0.0 37.3978 1 TACATGG 13375 0.0 36.303127 2 ACATGGG 13570 0.0 35.299107 3 ACGCAGA 7435 0.0 34.085804 7 CATGGGG 12780 0.0 31.847322 4 CGCAGAG 7985 0.0 31.738003 8 ATGGGGG 7390 0.0 29.872295 5 GTATAGG 880 0.0 27.72574 1 GCAGAGT 9095 0.0 27.667217 9 AGAGTAC 8110 0.0 23.985825 10-11 GTATATA 715 0.0 23.304188 1 CAGAGTA 8705 0.0 22.653877 10-11 TGGGGGG 5085 0.0 22.349325 6 >>END_MODULE