FastQCFastQC Report
Mon 15 Aug 2016
SRR2921350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535629
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.067.2460251
GTATCAA52700.057.3804321
TCAACGC71600.040.7037244
GTACATG126500.038.5971681
CAACGCA75100.038.568675
ATCAACG76550.038.1495253
TATCAAC78650.038.0402452
AACGCAG76850.037.6046686
TACATGG129650.037.2349552
ACATGGG136050.035.351943
CATGGGG126750.032.5384144
ATGGGGG72150.030.7287485
ACGCAGA94400.029.8472587
CGCAGAG99600.028.2269298
GCAGAGT111250.024.4659929
AGAGTAC101600.024.2099810-11
ATGGGGA49200.023.1369745
TGGGGGG51300.022.6496326
TATACCG3255.966285E-1022.005755
GTGGTAT14900.021.2575651
Produced by FastQC (version 0.11.5)