##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2535629
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.954759943193583	31.0	30.0	34.0	16.0	34.0
2	29.7417615116407	33.0	31.0	34.0	25.0	34.0
3	29.974015520409335	33.0	31.0	34.0	26.0	34.0
4	32.09735178135287	37.0	33.0	37.0	19.0	37.0
5	31.734411855993127	37.0	33.0	37.0	19.0	37.0
6	31.875073995446495	37.0	33.0	37.0	17.0	37.0
7	32.08390462484851	37.0	35.0	37.0	17.0	37.0
8	32.33879128216312	37.0	35.0	37.0	17.0	37.0
9	34.07998567613795	39.0	35.0	39.0	17.0	39.0
10-11	34.2646578028568	39.0	37.0	39.0	17.0	39.0
12-13	34.290946940581605	39.0	37.0	39.0	17.5	39.0
14-15	35.665459536864425	40.0	38.0	41.0	19.0	41.0
16-17	35.687168943090654	40.0	38.0	41.0	20.5	41.0
18-19	35.71407252401673	40.5	38.0	41.0	20.5	41.0
20-21	35.70585128975887	41.0	38.0	41.0	19.0	41.0
22-23	35.654567959271645	40.5	38.0	41.0	19.0	41.0
24-25	35.5897546525931	40.5	37.5	41.0	18.0	41.0
26-27	35.50068976967845	40.0	37.0	41.0	18.0	41.0
28-29	35.426380988701425	40.0	37.0	41.0	17.0	41.0
30-31	35.33407687007839	40.0	37.0	41.0	16.0	41.0
32-33	35.220588855861806	40.0	36.5	41.0	16.0	41.0
34-35	35.13910710123602	40.0	36.0	41.0	14.5	41.0
36-37	35.05524191433368	40.0	36.0	41.0	9.5	41.0
38-39	34.95500702192632	40.0	36.0	41.0	9.0	41.0
40-41	34.82563123390685	40.0	35.5	41.0	8.0	41.0
42-43	34.690949464610156	40.0	35.0	41.0	4.5	41.0
44-45	34.54639164483448	40.0	35.0	41.0	2.0	41.0
46-47	34.34312886467224	40.0	35.0	41.0	2.0	41.0
48-49	34.14950195789684	40.0	35.0	41.0	2.0	41.0
50-51	33.97319698583665	40.0	35.0	41.0	2.0	41.0
52-53	33.78854674717792	39.0	35.0	41.0	2.0	41.0
54-55	33.585319263977496	39.0	35.0	41.0	2.0	41.0
56-57	33.34495168654405	39.0	34.0	41.0	2.0	41.0
58-59	33.282726100703215	38.5	34.0	41.0	2.0	41.0
60-61	33.05234953536184	38.0	34.0	41.0	2.0	41.0
62-63	32.77731501729946	37.0	34.0	40.0	2.0	41.0
64-65	32.48455787498881	37.0	34.0	40.0	2.0	41.0
66-67	32.1756258506272	36.0	34.0	39.0	2.0	41.0
68-69	31.849356707941105	35.5	33.0	39.0	2.0	41.0
70-71	31.52429554954609	35.0	33.0	38.5	2.0	40.5
72-73	31.188559722262205	35.0	33.0	37.0	2.0	39.5
74-75	30.860356739885844	35.0	33.0	37.0	2.0	39.0
76-77	30.565973571054755	35.0	33.0	36.5	2.0	39.0
78-79	30.31148996954996	35.0	32.0	36.0	2.0	37.5
80-81	30.080775421009932	35.0	32.0	35.5	2.0	37.0
82-83	29.879332110494083	35.0	32.0	35.0	2.0	37.0
84-85	29.703704485159303	35.0	32.0	35.0	2.0	36.0
86-87	29.556326260663525	35.0	32.0	35.0	2.0	36.0
88-89	29.43485166796878	35.0	32.0	35.0	2.0	36.0
90-91	29.33066430459661	35.0	32.0	35.0	2.0	35.5
92-93	29.261148022837723	35.0	31.5	35.0	2.0	35.0
94-95	29.19006487147765	35.0	31.0	35.0	2.0	35.0
96-97	29.12695390374538	35.0	31.0	35.0	2.0	35.0
98-99	29.06590554059762	35.0	31.0	35.0	2.0	35.0
100-101	29.013251741481106	35.0	31.0	35.0	2.0	35.0
102-103	28.340915015564185	34.0	29.5	35.0	2.0	35.0
104-105	28.881998510034393	35.0	31.0	35.0	2.0	35.0
106-107	28.94702142939681	35.0	31.0	35.0	2.0	35.0
108-109	29.01766248138036	35.0	31.5	35.0	2.0	35.0
110-111	29.125890262337272	35.0	32.0	35.0	2.0	35.0
112-113	29.092941238643352	35.0	32.0	35.0	2.0	35.0
114-115	29.034083653405133	35.0	32.0	35.0	2.0	35.0
116-117	28.9736737906058	35.0	32.0	35.0	2.0	35.0
118-119	28.899216131381998	35.0	32.0	35.0	2.0	35.0
120-121	28.80646577239809	35.0	32.0	35.0	2.0	35.0
122-123	28.692298242369052	35.0	32.0	35.0	2.0	35.0
124-125	28.17279617798976	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	189371.0
3	11980.0
4	8544.0
5	7939.0
6	8302.0
7	8568.0
8	8987.0
9	9036.0
10	9182.0
11	9324.0
12	9958.0
13	11348.0
14	13074.0
15	10887.0
16	7542.0
17	6712.0
18	6088.0
19	5576.0
20	5601.0
21	5711.0
22	6627.0
23	8067.0
24	10703.0
25	13941.0
26	16263.0
27	19298.0
28	22904.0
29	27262.0
30	32846.0
31	40419.0
32	51542.0
33	69512.0
34	105796.0
35	182070.0
36	406958.0
37	853232.0
38	314060.0
39	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.039046720782345	18.16701186552641	12.304937577254158	27.489003836437085
2	15.186606557978315	20.020673371380436	38.07355098084144	26.71916908979981
3	17.9600012462391	24.418911441697507	28.495414747188963	29.12567256487443
4	13.119662097877383	17.220291167620974	31.824112920149005	37.835933814352636
5	14.485878840247823	37.69407927098853	32.14316616943021	15.676875719333431
6	32.844320792752725	35.04542963329189	16.74711435909435	15.363135214861035
7	29.296244137011485	31.66723866554634	19.65352647171367	19.38299072572851
8	27.262519065091684	34.70822866514185	18.51192300365269	19.517329266113776
9	27.539628386506433	15.997838630922564	18.382325680456567	38.08020730211443
10-11	26.229374686337476	25.909189954517085	25.749896226387193	22.11153913275825
12-13	26.315549592565556	23.26914349979283	26.34461259199337	24.070694315648243
14-15	23.833672039560994	24.076846415623105	23.807130301790995	28.282351243024905
16-17	23.904581466768207	26.88918607572322	24.425418702814962	24.78081375469361
18-19	24.146868489041577	26.05635524755396	26.113244484899013	23.68353177850545
20-21	25.107438824843854	24.986896742386207	25.715414202945304	24.19025022982463
22-23	24.89112169012107	24.668376170173158	25.51110592283019	24.929396216875578
24-25	24.40002066548379	25.25858475352664	25.440354247407644	24.90104033358192
26-27	24.179168166951868	25.248764704931205	25.731958421362116	24.840108706754812
28-29	24.24047445426756	25.396952787651507	25.556913097302488	24.80565966077845
30-31	24.631876350996144	25.36475170460663	25.450391204706996	24.55298073969023
32-33	24.219158244364614	25.492017956885647	25.564564058858767	24.724259739890968
34-35	24.478561335274208	25.48939533346558	25.748995614106008	24.283047717154204
36-37	24.62209574034687	25.48156690115155	25.571840359926473	24.324496998575107
38-39	24.515021716505057	25.581285748033327	25.63066205663368	24.273030478827938
40-41	24.36369831706452	25.73111050551954	25.46314938029183	24.442041797124105
42-43	24.310240181035947	25.66662946353745	25.419294384154778	24.603835971271824
44-45	24.32808585167625	25.65872215533108	25.51459618106592	24.498595811926744
46-47	24.299513059678684	25.668068948572525	25.193748770029057	24.83866922171974
48-49	24.236432064785504	25.888329877911946	25.09744130549067	24.777796751811877
50-51	24.253055159094647	25.901383838093032	25.21167331656169	24.63388768625063
52-53	24.42293411220648	25.815961246696578	25.233778285387963	24.52732635570898
54-55	24.04496083614756	25.512663721703767	25.470543995198035	24.971831446950638
56-57	24.11608717205869	25.66649143072587	25.201754673100833	25.01566672411461
58-59	24.286794322040013	25.6904105450758	25.294532441457328	24.72826269142686
60-61	24.516678110244047	25.47308774272577	25.397268291220836	24.612965855809346
62-63	24.25504677537605	25.559003308449302	25.530962928724982	24.65498698744966
64-65	24.17246371610358	25.28175454689941	25.54845365784979	24.997328079147223
66-67	24.134406098052988	25.776483862583998	25.306935675526667	24.78217436383635
68-69	24.121273261979574	25.593531230318	25.310859751170224	24.974335756532206
70-71	24.086252365783796	25.42597911602999	25.480995050932137	25.006773467254085
72-73	24.210639647992668	25.75798746583195	25.140369509892814	24.891003376282573
74-75	23.999488884217683	25.740753083357227	25.284475765184887	24.9752822672402
76-77	23.96632157149173	25.349824441982637	25.47283139607569	25.211022590449943
78-79	23.949895666913417	25.43256525303978	25.42808904614989	25.189450033896915
80-81	24.125966377573373	25.666728058402867	25.12650707181532	25.080798492208444
82-83	24.048766597952618	25.717386100253627	25.19672633496462	25.03712096682914
84-85	24.042929781919987	25.673195881574156	25.1341580333716	25.14971630313425
86-87	23.760080832014463	25.919742202033497	25.189568347735413	25.130608618216627
88-89	23.879222867383202	25.613684020809036	25.186531625880598	25.32056148592716
90-91	23.94803876446835	25.438442890321543	25.274045293046555	25.33947305216355
92-93	23.89346973666178	25.70028799690805	25.290111777505874	25.11613048892429
94-95	23.89099421957567	25.54632443967508	25.233877064726002	25.32880427602325
96-97	23.749338425583034	25.606203300339402	25.188277571779345	25.456180702298216
98-99	23.60815079969325	25.562064789853878	25.215996744002524	25.61378766645035
100-101	23.619543710850444	25.613999524378368	25.30200593225586	25.464450832515322
102-103	23.513756056930262	25.477825320231258	25.383312263606438	25.625106359232042
104-105	23.52576491390596	25.614990524045616	25.26614210244127	25.593102459607152
106-107	23.573403711787766	25.457121115527947	25.270520142079395	25.698955030604886
108-109	23.507991054640954	25.418611213485953	25.38118441806953	25.692213313803563
110-111	23.39243268995278	25.847811400538806	25.143150562565246	25.61660534694317
112-113	23.39396905631848	25.915239608354057	25.17756599850963	25.51322533681784
114-115	23.336295132435016	25.863743043617323	25.13398098532864	25.665980838619017
116-117	23.161073666691646	25.71091374462161	25.276028253556344	25.8519843351304
118-119	23.245798511961276	25.58201260086539	25.271634801672725	25.900554085500605
120-121	23.10903772518592	25.698101495212033	25.372481835853616	25.82037894374843
122-123	23.066323182351677	25.827718584752073	25.194787473023965	25.91117075987228
124-125	22.922826795339617	25.771924306705145	25.09400533004158	26.211243567913662
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	64.0
2	69.5
3	79.5
4	76.0
5	101.5
6	187.0
7	447.0
8	654.0
9	721.5
10	682.0
11	593.5
12	645.5
13	706.0
14	786.0
15	887.5
16	998.0
17	1078.5
18	1190.0
19	1324.0
20	1437.0
21	1564.5
22	1729.5
23	2021.5
24	2383.0
25	2815.0
26	3427.5
27	4319.5
28	5478.0
29	7062.0
30	9438.5
31	12551.5
32	16576.0
33	21025.5
34	25527.0
35	30346.5
36	36211.5
37	42058.5
38	47221.5
39	52790.0
40	57850.5
41	62131.5
42	67059.5
43	74988.0
44	83028.5
45	90673.0
46	96046.5
47	99710.0
48	106919.0
49	112958.0
50	113730.0
51	112902.0
52	112007.0
53	110706.0
54	111173.5
55	108821.5
56	101116.0
57	92105.5
58	84930.5
59	77481.5
60	65803.0
61	54460.0
62	45614.5
63	35425.0
64	26437.0
65	20632.0
66	15976.5
67	12269.5
68	9509.0
69	7305.0
70	5779.0
71	4638.0
72	3686.5
73	3119.0
74	2486.0
75	1884.5
76	1459.5
77	990.5
78	687.0
79	508.0
80	405.0
81	333.0
82	271.0
83	247.5
84	221.5
85	199.5
86	184.0
87	167.0
88	166.5
89	163.0
90	141.0
91	133.0
92	137.5
93	119.5
94	107.0
95	105.5
96	93.5
97	64.5
98	35.0
99	14.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3575444199447159
2	0.0
3	0.0
4	0.01802314139805153
5	0.18299207021216432
6	0.0500861916313467
7	0.025989606523667302
8	0.009307355295273875
9	0.007848151287116531
10-11	0.0024254336892345052
12-13	0.05832872238012738
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.155035693313178E-4
92-93	4.535363809137693E-4
94-95	7.887589233282945E-5
96-97	4.3381740783056193E-4
98-99	1.971897308320736E-5
100-101	0.0
102-103	1.971897308320736E-5
104-105	1.971897308320736E-5
106-107	1.7747075774886625E-4
108-109	4.929743270801841E-4
110-111	4.7325535399697665E-4
112-113	8.873537887443313E-4
114-115	3.155035693313178E-4
116-117	7.493209771618798E-4
118-119	3.746604885809399E-4
120-121	7.887589233282945E-5
122-123	0.0017747075774886625
124-125	5.915691924962208E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2535629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.305256209848835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37209414088636	48.25792891357205
2	9.759568557909365	11.575874276487996
3	3.301180791327165	5.873321178740671
4	1.6071169153438702	3.8124192169460067
5	0.9305107832025828	2.759209020192814
6	0.6164219865965685	2.19342383090961
7	0.4204112262837876	1.7452816841779728
8	0.31973504509992856	1.5169575015135068
9	0.2415203635866772	1.2891084338163363
>10	1.3262475677754362	14.082842711989166
>50	0.07271314258731361	2.9305676567715264
>100	0.031085305203513693	3.1716820770137004
>500	8.630602175266704E-4	0.3414760734838676
>1k	5.311139800147838E-4	0.44990742438490483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1831383849924418E-4
7	0.0	0.0	0.0	0.0	1.1831383849924418E-4
8	0.0	0.0	0.0	0.0	1.1831383849924418E-4
9	0.0	0.0	0.0	0.0	1.577517846656589E-4
10-11	0.0	0.0	0.0	0.0	1.577517846656589E-4
12-13	0.0	0.0	0.0	0.0	1.577517846656589E-4
14-15	0.0	0.0	0.0	0.0	1.971897308320736E-4
16-17	0.0	0.0	0.0	0.0	1.971897308320736E-4
18-19	0.0	0.0	0.0	0.0	1.971897308320736E-4
20-21	0.0	0.0	0.0	0.0	1.971897308320736E-4
22-23	0.0	0.0	0.0	3.943794616641472E-5	2.563466500816957E-4
24-25	0.0	0.0	0.0	2.7606562316490307E-4	3.155035693313178E-4
26-27	0.0	0.0	0.0	4.929743270801841E-4	3.155035693313178E-4
28-29	0.0	0.0	0.0	0.0016366747659062111	3.549415154977325E-4
30-31	0.0	0.0	0.0	0.0062509144673767334	3.9437946166414727E-4
32-33	0.0	0.0	0.0	0.016800565066892672	4.33817407830562E-4
34-35	1.971897308320736E-5	0.0	0.0	0.03127429130996688	4.33817407830562E-4
36-37	3.943794616641472E-5	0.0	0.0	0.05322150835157667	4.33817407830562E-4
38-39	3.943794616641472E-5	0.0	0.0	0.092146761217828	4.33817407830562E-4
40-41	3.943794616641472E-5	0.0	0.0	0.138170844394034	4.33817407830562E-4
42-43	3.943794616641472E-5	0.0	0.0	0.1834850445392445	4.33817407830562E-4
44-45	3.943794616641472E-5	0.0	0.0	0.23394589665917215	4.33817407830562E-4
46-47	3.943794616641472E-5	0.0	0.0	0.2862208943027549	4.535363809137693E-4
48-49	3.943794616641472E-5	0.0	0.0	0.34165092763965077	4.732553539969767E-4
50-51	3.943794616641472E-5	0.0	0.0	0.40504742610216243	5.126933001633914E-4
52-53	3.943794616641472E-5	0.0	0.0	0.4694890301380841	5.126933001633914E-4
54-55	3.943794616641472E-5	0.0	0.0	0.5383871220908105	5.324122732465988E-4
56-57	3.943794616641472E-5	0.0	0.0	0.6102036220598518	5.718502194130135E-4
58-59	3.943794616641472E-5	0.0	0.0	0.6932599366863212	5.915691924962209E-4
60-61	3.943794616641472E-5	0.0	0.0	0.7822122242646696	5.915691924962209E-4
62-63	3.943794616641472E-5	0.0	0.0	0.87818446626064	6.310071386626356E-4
64-65	3.943794616641472E-5	0.0	0.0	0.9697396582859716	6.310071386626356E-4
66-67	3.943794616641472E-5	0.0	0.0	1.0696754138716664	6.310071386626356E-4
68-69	3.943794616641472E-5	0.0	0.0	1.1824482209345293	6.310071386626356E-4
70-71	3.943794616641472E-5	0.0	0.0	1.3065594375202365	6.310071386626356E-4
72-73	3.943794616641472E-5	0.0	0.0	1.4300199279941979	9.070727618275386E-4
74-75	3.943794616641472E-5	0.0	0.0	1.5580157822772969	9.465107079939534E-4
76-77	3.943794616641472E-5	0.0	0.0	1.6998543556648076	9.662296810771608E-4
78-79	3.943794616641472E-5	0.0	0.0	1.860327358615949	9.859486541603682E-4
80-81	3.943794616641472E-5	0.0	0.0	2.0315866398436047	0.0010253866003267827
82-83	3.943794616641472E-5	0.0	0.0	2.213158943993778	0.0011831383849924418
84-85	3.943794616641472E-5	0.0	0.0	2.398694761733676	0.001202857358075649
86-87	3.943794616641472E-5	0.0	0.0	2.5951746095347543	0.0012225763311588564
88-89	3.943794616641472E-5	0.0	0.0	2.8154749768203473	0.0012225763311588564
90-91	3.943794616641472E-5	0.0	0.0	3.0580183457437977	0.0012225763311588564
92-93	3.943794616641472E-5	0.0	0.0	3.3128663538711693	0.0012225763311588564
94-95	3.943794616641472E-5	0.0	0.0	3.5739061195466686	0.0013803281158245152
96-97	3.943794616641472E-5	0.0	0.0	3.8528704317548033	0.001538079900490174
98-99	5.915691924962209E-5	0.0	0.0	4.153683366139132	0.0016169557928230036
100-101	7.887589233282945E-5	0.0	0.0	4.484666329340767	0.0016169557928230036
102-103	7.887589233282945E-5	0.0	0.0	4.834895010271612	0.00179442655057187
104-105	7.887589233282945E-5	1.971897308320736E-5	0.0	5.1995185415532	0.001853583469821492
106-107	7.887589233282945E-5	3.943794616641472E-5	0.0	5.5856554724685665	0.001853583469821492
108-109	7.887589233282945E-5	3.943794616641472E-5	0.0	5.991294467763225	0.0018733024429046993
110-111	7.887589233282945E-5	3.943794616641472E-5	0.0	6.42789225079852	0.0019324593621543214
112-113	7.887589233282945E-5	3.943794616641472E-5	0.0	6.891366994146226	0.0019324593621543214
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2675	0.0	67.246025	1
GTATCAA	5270	0.0	57.380432	1
TCAACGC	7160	0.0	40.703724	4
GTACATG	12650	0.0	38.597168	1
CAACGCA	7510	0.0	38.56867	5
ATCAACG	7655	0.0	38.149525	3
TATCAAC	7865	0.0	38.040245	2
AACGCAG	7685	0.0	37.604668	6
TACATGG	12965	0.0	37.234955	2
ACATGGG	13605	0.0	35.35194	3
CATGGGG	12675	0.0	32.538414	4
ATGGGGG	7215	0.0	30.728748	5
ACGCAGA	9440	0.0	29.847258	7
CGCAGAG	9960	0.0	28.226929	8
GCAGAGT	11125	0.0	24.465992	9
AGAGTAC	10160	0.0	24.20998	10-11
ATGGGGA	4920	0.0	23.136974	5
TGGGGGG	5130	0.0	22.649632	6
TATACCG	325	5.966285E-10	22.00575	5
GTGGTAT	1490	0.0	21.257565	1
>>END_MODULE