FastQCFastQC Report
Mon 15 Aug 2016
SRR2921349_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921349_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982433
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34100.059.6835751
GGTATCA17800.055.4972151
TCAACGC42150.047.01194
CAACGCA43450.045.331425
AACGCAG46100.042.982686
TATCAAC46150.042.93722
ATCAACG47550.041.673013
GTACATG97800.036.386931
ACGCAGA57000.034.6509257
TACATGG101600.034.4971122
ACATGGG102300.033.795723
CGCAGAG60650.032.662858
CATGGGG93750.031.9269814
ATGGGGG61150.030.1666015
GCAGAGT67250.028.4834929
TGGGGGG47350.026.7676246
AGAGTAC61050.024.65270210-11
GTACTAG3302.910383E-1123.4429631
CAGAGTA65250.022.15416710-11
GAGTACA68800.020.7589741
Produced by FastQC (version 0.11.5)