FastQCFastQC Report
Mon 15 Aug 2016
SRR2921349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982433
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.067.175961
GTATCAA42550.056.6121641
TCAACGC56050.041.8927734
CAACGCA58050.040.3467755
ATCAACG59250.040.032553
TATCAAC60650.039.992832
AACGCAG60250.038.7697226
GTACATG91250.036.879061
TACATGG95150.035.450812
ACATGGG98650.034.0722243
CATGGGG89300.032.8346334
ACGCAGA73550.031.5080017
ATGGGGG58100.031.0802865
CGCAGAG78550.029.1953478
TACCGTA1454.029873E-628.7596767
GCAGAGT84050.025.440349
TGGGGGG46050.025.2329856
AGAGTAC77100.024.02457810-11
GTGGTAT11800.023.30131
GAGTACT34200.022.45203612-13
Produced by FastQC (version 0.11.5)