##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1982433
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.851549585786756	31.0	30.0	34.0	16.0	34.0
2	29.634366457781926	33.0	31.0	34.0	25.0	34.0
3	29.868384959289923	33.0	31.0	34.0	25.0	34.0
4	31.98525397831856	37.0	33.0	37.0	19.0	37.0
5	31.626274885456407	37.0	33.0	37.0	17.0	37.0
6	31.77079528034491	37.0	33.0	37.0	17.0	37.0
7	31.97989288919222	37.0	35.0	37.0	17.0	37.0
8	32.235235692706894	37.0	35.0	37.0	17.0	37.0
9	33.95855446312687	39.0	35.0	39.0	17.0	39.0
10-11	34.14122343605055	39.0	36.0	39.0	17.0	39.0
12-13	34.15749485606828	39.0	37.0	39.0	17.0	39.0
14-15	35.51334622658118	40.0	37.0	41.0	17.0	41.0
16-17	35.53545718821267	40.0	37.0	41.0	17.0	41.0
18-19	35.56412751401939	40.0	37.5	41.0	17.5	41.0
20-21	35.55515747568771	40.0	37.5	41.0	18.0	41.0
22-23	35.50235922222844	40.0	37.0	41.0	18.0	41.0
24-25	35.433517299197504	40.0	37.0	41.0	17.5	41.0
26-27	35.33914412239909	40.0	37.0	41.0	16.0	41.0
28-29	35.26227468973731	40.0	37.0	41.0	15.5	41.0
30-31	35.166273462961925	40.0	36.5	41.0	13.5	41.0
32-33	35.038650486548605	40.0	36.0	41.0	9.5	41.0
34-35	34.94951153456384	40.0	36.0	41.0	9.0	41.0
36-37	34.86531348096001	40.0	36.0	41.0	8.0	41.0
38-39	34.765714906884625	40.0	35.0	41.0	5.0	41.0
40-41	34.6326178488756	40.0	35.0	41.0	2.0	41.0
42-43	34.486812164648185	40.0	35.0	41.0	2.0	41.0
44-45	34.33761292311014	40.0	35.0	41.0	2.0	41.0
46-47	34.13511957276741	40.0	35.0	41.0	2.0	41.0
48-49	33.93068618208031	40.0	35.0	41.0	2.0	41.0
50-51	33.75448527138118	39.5	35.0	41.0	2.0	41.0
52-53	33.57137794820808	39.0	35.0	41.0	2.0	41.0
54-55	33.37637085339075	39.0	34.0	41.0	2.0	41.0
56-57	33.1377693470599	38.5	34.0	41.0	2.0	41.0
58-59	33.07990787078302	38.5	34.0	41.0	2.0	41.0
60-61	32.84670806024718	37.5	34.0	41.0	2.0	41.0
62-63	32.56929010967836	37.0	34.0	40.0	2.0	41.0
64-65	32.2815431845616	36.5	34.0	40.0	2.0	41.0
66-67	31.974052086501786	36.0	33.0	39.0	2.0	41.0
68-69	31.651303221849112	35.5	33.0	39.0	2.0	41.0
70-71	31.325990336117286	35.0	33.0	38.5	2.0	40.5
72-73	30.98760311193367	35.0	33.0	37.0	2.0	39.5
74-75	30.655864788368635	35.0	32.5	37.0	2.0	39.0
76-77	30.360312303114405	35.0	32.0	36.5	2.0	39.0
78-79	30.097087770431585	35.0	32.0	36.0	2.0	38.0
80-81	29.866907229651645	35.0	32.0	35.5	2.0	37.0
82-83	29.65874256532251	35.0	32.0	35.0	2.0	37.0
84-85	29.485008068368515	35.0	32.0	35.0	2.0	36.0
86-87	29.336951614506013	35.0	31.0	35.0	2.0	36.0
88-89	29.21143110511175	35.0	31.0	35.0	2.0	36.0
90-91	29.106036118244603	35.0	31.0	35.0	2.0	35.5
92-93	29.033196077748908	35.0	31.0	35.0	2.0	35.0
94-95	28.958683597377565	35.0	31.0	35.0	2.0	35.0
96-97	28.892786288363844	35.0	31.0	35.0	2.0	35.0
98-99	28.837183148182056	35.0	31.0	35.0	2.0	35.0
100-101	28.77595711935788	35.0	31.0	35.0	2.0	35.0
102-103	28.1040799361189	34.0	29.5	35.0	2.0	35.0
104-105	28.647705622333767	35.0	30.5	35.0	2.0	35.0
106-107	28.705154222109904	35.0	31.0	35.0	2.0	35.0
108-109	28.77367053514545	35.0	31.0	35.0	2.0	35.0
110-111	28.87930159556464	35.0	31.5	35.0	2.0	35.0
112-113	28.837538015156124	35.0	31.5	35.0	2.0	35.0
114-115	28.773109103813347	35.0	31.0	35.0	2.0	35.0
116-117	28.71152492921577	35.0	31.0	35.0	2.0	35.0
118-119	28.62809033142608	35.0	31.0	35.0	2.0	35.0
120-121	28.52741202350849	35.0	31.0	35.0	2.0	35.0
122-123	28.40175708334153	35.0	31.0	35.0	2.0	35.0
124-125	27.87345095647621	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	151576.0
3	10138.0
4	7223.0
5	6686.0
6	6774.0
7	7139.0
8	7535.0
9	7301.0
10	7620.0
11	7775.0
12	8299.0
13	9541.0
14	10941.0
15	9361.0
16	6488.0
17	5623.0
18	5123.0
19	4720.0
20	4487.0
21	4783.0
22	5349.0
23	6653.0
24	8680.0
25	11147.0
26	13207.0
27	15200.0
28	18251.0
29	21500.0
30	26325.0
31	32194.0
32	40932.0
33	55039.0
34	84451.0
35	142657.0
36	312747.0
37	657011.0
38	241594.0
39	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37043351575563	18.172034465006252	12.07277520649694	27.38475681274118
2	15.422664977832795	20.171728376192284	37.808793538041385	26.596813107933535
3	18.222658722892525	24.339838975642554	28.776458019009976	28.661044282454945
4	13.287311298051455	17.260785815405324	31.80338571228206	37.64851717426116
5	14.468792557613058	37.69704411716403	32.202775735433455	15.631387589789458
6	32.70008261547367	34.97950258116579	16.87495489457569	15.445459908784844
7	28.987489625142853	31.983521321119802	19.614269748808628	19.414719304928717
8	27.548070253322603	34.452429177880745	18.584238657488566	19.415261911308082
9	27.620378451185246	15.85131337997972	18.428131100898458	38.10017706793658
10-11	26.29577373136527	25.944606174273744	25.773776765081692	21.985843329279298
12-13	26.323022840891024	23.22043693203756	26.378692938780556	24.077847288290858
14-15	23.865825478086773	24.073474362059148	24.00724261551336	28.053457544340716
16-17	23.885220837223756	26.801284078705308	24.59187775828994	24.721617325780997
18-19	24.082982880127602	26.1211097676441	26.168778465653062	23.627128886575232
20-21	24.91496560035068	25.176084134999773	26.037954372228466	23.87099589242108
22-23	24.7760706162579	24.743938382785196	25.797719267183304	24.6822717337736
24-25	24.340671286242713	25.055878307110508	25.74604034537359	24.857410061273193
26-27	23.967922245039304	25.326127036827977	26.0024676748218	24.70348304331092
28-29	24.076727939859758	25.616906094682644	25.768790168444532	24.537575797013066
30-31	24.677353534772674	25.304613068890603	25.711512066233766	24.30652133010296
32-33	24.313280701037563	25.468754807854793	25.74720053590714	24.470763955200503
34-35	24.44945175952983	25.57100290400735	25.940145265943414	24.039400070519406
36-37	24.567538978618696	25.463660058120503	25.948468371944976	24.020332591315825
38-39	24.36087373444651	25.736632713438485	25.834567927390236	24.06792562472477
40-41	24.402211827587617	25.719658621501964	25.655268046889855	24.222861504020564
42-43	24.187349585080558	25.786621792514552	25.543208774268788	24.482819848136103
44-45	24.090650226262376	25.710023995766818	25.857519522727877	24.341806255242926
46-47	24.078342117993394	25.654032191756293	25.448880239584387	24.818745450665926
48-49	24.133476389870427	25.932704913608685	25.32204114842721	24.61177754809368
50-51	24.142732692605502	26.006023911022464	25.451478057518212	24.399765338853825
52-53	24.26036592409428	25.780820839846797	25.510244230195926	24.448569005862996
54-55	23.760979564000397	25.5365250679342	25.833357293790005	24.869138074275398
56-57	23.882648240823272	25.706013771966063	25.485602792124627	24.925735195086038
58-59	24.114913341333605	25.730125557837262	25.55372615367077	24.601234947158364
60-61	24.4322758953266	25.568278978406838	25.72255909783584	24.276886028430724
62-63	24.160766088942225	25.574029488007916	25.731941508237604	24.533262914812255
64-65	23.916268544762925	25.314474688425786	25.77554953937914	24.99370722743215
66-67	23.97145325970663	25.58406765827647	25.59037304161099	24.854106040405906
68-69	23.81233060587672	25.640992659020505	25.67647935642718	24.870197378675595
70-71	23.81376823327699	25.46918357392154	25.707653171633037	25.009395021168434
72-73	23.881815930223116	25.84092375379143	25.358486264100726	24.91877405188473
74-75	23.800325156007794	25.797063507316516	25.529690032399582	24.87292130427611
76-77	23.789782555072478	25.329582386895293	25.531808641199984	25.34882641683225
78-79	23.647583550112415	25.494430328792955	25.692646359296884	25.16533976179775
80-81	23.93667276523343	25.68336483502847	25.313894593159013	25.06606780657909
82-83	23.925701398231368	25.638899271753445	25.532943610200192	24.902455719814995
84-85	23.880781847356253	25.647953802221817	25.295457652288878	25.17580669813305
86-87	23.443944889940795	25.9772965845504	25.41387275131114	25.164885774197664
88-89	23.47637978181356	25.62717125875124	25.527041771399084	25.369407188036114
90-91	23.72161344369954	25.388834564439968	25.57660911694004	25.31294287492046
92-93	23.832730115893412	25.44197132300087	25.52366419915003	25.201634361955687
94-95	23.75998868056727	25.469303731301686	25.3833739022004	25.387333685930635
96-97	23.450509628102132	25.373179124971124	25.522188470086654	25.654122776840094
98-99	23.252777396652196	25.370327960807735	25.495048506077385	25.881846136462688
100-101	23.36656522565958	25.562881570272488	25.509613691862477	25.560939512205454
102-103	23.233653604851614	25.39985598500832	25.553656934094853	25.81283347604521
104-105	23.299431380387478	25.471005948500135	25.402151145120953	25.82741152599143
106-107	23.400839676135007	25.279694476125524	25.477809820367014	25.841656027372455
108-109	23.316631558132872	25.224926655448076	25.539137944254104	25.919303842164947
110-111	23.079637312886994	25.67769272481935	25.537233438843842	25.705436523449816
112-113	23.051996202609544	25.801447124566312	25.433057877920557	25.71349879490359
114-115	23.084301358509848	25.75604832353433	25.3404723850205	25.81917793293532
116-117	22.849634713953005	25.609268272751624	25.441417783021652	26.099679230273722
118-119	22.929572761496125	25.3978326588241	25.540965149319344	26.13162943036043
120-121	22.879176405338594	25.416010591034293	25.67554036545525	26.029272638171864
122-123	22.822918863076207	25.567014157572483	25.41661580244572	26.19345117690559
124-125	22.557985624720356	25.54325976540924	25.24125682054003	26.65749778933037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	78.5
2	84.5
3	98.0
4	93.0
5	115.5
6	203.5
7	434.0
8	620.0
9	702.5
10	707.5
11	649.5
12	685.0
13	738.0
14	781.0
15	889.5
16	1010.0
17	1087.5
18	1158.0
19	1227.0
20	1330.0
21	1453.0
22	1612.5
23	1845.0
24	2137.5
25	2527.5
26	3074.5
27	3901.0
28	5052.5
29	6591.5
30	8496.5
31	10813.0
32	13855.0
33	16973.0
34	20276.0
35	24053.0
36	28010.0
37	32297.0
38	36525.5
39	40834.5
40	45366.0
41	49551.0
42	53664.0
43	58342.5
44	63446.5
45	68362.5
46	73567.0
47	78920.5
48	83725.5
49	87324.5
50	89415.5
51	90938.0
52	90510.0
53	88932.0
54	87542.5
55	84857.0
56	79509.5
57	72312.0
58	65173.5
59	57507.0
60	48923.0
61	41128.5
62	34511.0
63	27110.0
64	20221.0
65	15328.5
66	11723.0
67	9174.5
68	7090.5
69	5413.0
70	4247.5
71	3280.5
72	2481.0
73	1989.0
74	1592.0
75	1250.5
76	940.5
77	654.5
78	469.0
79	354.0
80	302.5
81	252.0
82	213.5
83	190.0
84	174.0
85	157.5
86	145.0
87	143.5
88	133.5
89	119.0
90	110.0
91	102.5
92	91.5
93	84.5
94	77.5
95	72.0
96	59.5
97	42.0
98	34.0
99	15.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35209260539952675
2	0.0
3	0.0
4	0.01710019960321484
5	0.17644984723317259
6	0.04862711627580856
7	0.024111785871199682
8	0.008978865868354693
9	0.007213358534689444
10-11	0.0023203810671028986
12-13	0.056395348543935665
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.2610766669037492E-4
92-93	4.035445334091997E-4
94-95	7.566460001422494E-5
96-97	3.5310146673304976E-4
98-99	5.0443066676149964E-5
100-101	0.0
102-103	2.5221533338074982E-5
104-105	2.5221533338074982E-5
106-107	1.0088613335229993E-4
108-109	4.539876000853496E-4
110-111	4.035445334091997E-4
112-113	9.584182668468492E-4
114-115	1.7655073336652488E-4
116-117	7.062029334660995E-4
118-119	2.0177226670459986E-4
120-121	7.566460001422494E-5
122-123	0.0015637350669606487
124-125	1.0088613335229993E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1982433.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.031281291806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.03964998363263	54.832144864423604
2	9.319320625998504	12.307333634076734
3	3.0413096114594573	6.024647113492579
4	1.3995983929456874	3.696691007206252
5	0.8153597317118322	2.6919623899337735
6	0.5029266808172207	1.992533587811774
7	0.3746405500730006	1.7316596881631063
8	0.26183404841378516	1.38313901620664
9	0.20328039430801043	1.2080578407895328
>10	0.9977638107243172	11.259643364189976
>50	0.03408034277895901	1.4973966864083426
>100	0.009854306419965389	1.0124751234678013
>500	7.630414331333369E-5	0.04670652745240744
>1k	3.0521657325333475E-4	0.3156091563774328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0443066676149964E-5
2	0.0	0.0	0.0	5.0443066676149964E-5	5.0443066676149964E-5
3	0.0	0.0	0.0	1.0088613335229993E-4	5.0443066676149964E-5
4	0.0	0.0	0.0	1.0088613335229993E-4	5.0443066676149964E-5
5	0.0	0.0	0.0	1.0088613335229993E-4	5.0443066676149964E-5
6	0.0	0.0	0.0	1.0088613335229993E-4	5.0443066676149964E-5
7	0.0	0.0	0.0	1.0088613335229993E-4	5.0443066676149964E-5
8	0.0	0.0	0.0	1.513292000284499E-4	1.0088613335229993E-4
9	0.0	0.0	0.0	1.513292000284499E-4	2.0177226670459986E-4
10-11	0.0	0.0	0.0	2.0177226670459986E-4	2.0177226670459986E-4
12-13	0.0	0.0	0.0	2.5221533338074984E-4	2.2699380004267484E-4
14-15	0.0	0.0	0.0	2.5221533338074984E-4	3.278799333949748E-4
16-17	0.0	0.0	0.0	2.5221533338074984E-4	3.5310146673304976E-4
18-19	0.0	0.0	0.0	3.026584000568998E-4	4.035445334091997E-4
20-21	0.0	0.0	0.0	3.278799333949748E-4	4.035445334091997E-4
22-23	0.0	0.0	0.0	3.783230000711247E-4	5.296522000995746E-4
24-25	0.0	0.0	0.0	5.548737334376496E-4	7.062029334660995E-4
26-27	0.0	0.0	0.0	0.0010088613335229994	8.070890668183994E-4
28-29	0.0	0.0	0.0	0.002925697867216698	8.070890668183994E-4
30-31	0.0	0.0	0.0	0.009886841068525392	8.070890668183994E-4
32-33	0.0	0.0	0.0	0.027163591405106755	8.827536668326243E-4
34-35	0.0	0.0	0.0	0.049207211542584284	0.0010340828668610742
36-37	0.0	0.0	0.0	0.08199520488208176	0.0010593044001991493
38-39	0.0	0.0	0.0	0.14293042942687093	0.0010593044001991493
40-41	0.0	0.0	0.0	0.21261752603997208	0.0011097474668752992
42-43	0.0	0.0	0.0	0.27531825791842646	0.0011097474668752992
44-45	0.0	0.0	0.0	0.3461655450650791	0.001134969000213374
46-47	0.0	0.0	0.0	0.42566381814669146	0.001210633600227599
48-49	0.0	0.0	0.0	0.5074068076953925	0.0014376274002702739
50-51	0.0	0.0	0.0	0.5950516360452031	0.001462848933608349
52-53	0.0	0.0	0.0	0.6844871932620169	0.0015132920002844988
54-55	0.0	0.0	0.0	0.7848436744142173	0.0015889566002987238
56-57	0.0	0.0	0.0	0.8905723421674276	0.0016141781336367989
58-59	0.0	0.0	0.0	1.008079465989519	0.0016646212003129488
60-61	0.0	0.0	0.0	1.1293950413456595	0.0017150642669890987
62-63	0.0	0.0	0.0	1.2575960953030947	0.0018159504003413987
64-65	0.0	0.0	0.0	1.3873861058608286	0.0018159504003413987
66-67	0.0	0.0	0.0	1.5304678644877279	0.0018411719336794735
68-69	0.0	0.0	0.0	1.6897922905843474	0.0018663934670175486
70-71	0.0	0.0	0.0	1.8601889698163823	0.0018663934670175486
72-73	0.0	0.0	0.0	2.027861723447905	0.0020681657337221484
74-75	0.0	0.0	0.0	2.2027478356141166	0.0020681657337221484
76-77	0.0	0.0	0.0	2.404646209985407	0.0020681657337221484
78-79	0.0	0.0	0.0	2.6217783904928944	0.0020681657337221484
80-81	0.0	0.0	0.0	2.8413066166674987	0.0020933872670602233
82-83	0.0	0.0	0.0	3.0716548806441377	0.002244716467088673
84-85	0.0	0.0	0.0	3.315042677356561	0.0022699380004267485
86-87	0.0	0.0	0.0	3.5669805738705924	0.0022699380004267485
88-89	0.0	0.0	0.0	3.8592729237255434	0.0022699380004267485
90-91	0.0	0.0	0.0	4.171439841850898	0.0022699380004267485
92-93	0.0	0.0	0.0	4.49581398211188	0.002320381067102898
94-95	0.0	0.0	0.0	4.82114654063971	0.0024212672004551984
96-97	0.0	0.0	0.0	5.1685731623716915	0.0024969318004694234
98-99	0.0	0.0	0.0	5.548459897509778	0.0025725964004836483
100-101	0.0	0.0	0.0	5.97122323932259	0.002623039467159798
102-103	0.0	0.0	0.0	6.414441244672582	0.002698704067174023
104-105	0.0	0.0	0.0	6.875339544892563	0.002723925600512098
106-107	0.0	0.0	0.0	7.3593659911835605	0.002723925600512098
108-109	0.0	0.0	0.0	7.860442194011096	0.002723925600512098
110-111	0.0	0.0	0.0	8.402932154579752	0.002850033267202473
112-113	0.0	0.0	0.0	8.961891776418168	0.002976140933892848
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2180	0.0	67.17596	1
GTATCAA	4255	0.0	56.612164	1
TCAACGC	5605	0.0	41.892773	4
CAACGCA	5805	0.0	40.346775	5
ATCAACG	5925	0.0	40.03255	3
TATCAAC	6065	0.0	39.99283	2
AACGCAG	6025	0.0	38.769722	6
GTACATG	9125	0.0	36.87906	1
TACATGG	9515	0.0	35.45081	2
ACATGGG	9865	0.0	34.072224	3
CATGGGG	8930	0.0	32.834633	4
ACGCAGA	7355	0.0	31.508001	7
ATGGGGG	5810	0.0	31.080286	5
CGCAGAG	7855	0.0	29.195347	8
TACCGTA	145	4.029873E-6	28.759676	7
GCAGAGT	8405	0.0	25.44034	9
TGGGGGG	4605	0.0	25.232985	6
AGAGTAC	7710	0.0	24.024578	10-11
GTGGTAT	1180	0.0	23.3013	1
GAGTACT	3420	0.0	22.452036	12-13
>>END_MODULE