FastQCFastQC Report
Mon 15 Aug 2016
SRR2921348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096575
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.062.3308141
GTATCAA42750.059.5800171
TCAACGC54700.045.6902854
CAACGCA56950.043.8840875
GTACATG134900.043.452751
ATCAACG58200.043.0448273
TACATGG139550.041.6180652
ACATGGG139300.041.5218853
AACGCAG60900.041.232186
TATCAAC62450.040.4965822
CATGGGG119950.039.488874
ATGGGGG78800.037.4547585
ACGCAGA73750.033.9600267
CGCAGAG78700.031.7484478
TGGGGGG58050.031.6736016
GCAGAGT89450.027.932299
GTATAGG6300.027.3936821
TATACCG2256.552E-926.4465485
ATGGGGA33550.025.007955
AGAGTAC80550.024.51912310-11
Produced by FastQC (version 0.11.5)