##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921348_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2096575 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.39173175297807 33.0 31.0 34.0 26.0 34.0 2 30.453932246640353 33.0 31.0 34.0 26.0 34.0 3 30.457541466439313 33.0 31.0 34.0 26.0 34.0 4 33.810426052013405 37.0 35.0 37.0 30.0 37.0 5 33.89305462480474 37.0 35.0 37.0 30.0 37.0 6 33.93902436116046 37.0 35.0 37.0 30.0 37.0 7 33.85772319139549 37.0 35.0 37.0 30.0 37.0 8 33.869150877025625 37.0 35.0 37.0 30.0 37.0 9 35.44218594612642 39.0 37.0 39.0 30.0 39.0 10-11 35.439979967327666 39.0 37.0 39.0 30.0 39.0 12-13 35.389546999272625 39.0 37.0 39.0 30.0 39.0 14-15 36.699131440563775 40.5 38.0 41.0 29.0 41.0 16-17 36.71501377246223 41.0 38.0 41.0 29.0 41.0 18-19 36.71641987527276 41.0 38.0 41.0 29.0 41.0 20-21 36.72644694322884 41.0 38.0 41.0 29.0 41.0 22-23 36.703077400045316 41.0 38.0 41.0 29.0 41.0 24-25 36.6990572719793 41.0 38.0 41.0 29.0 41.0 26-27 36.65967733088489 40.5 38.0 41.0 29.5 41.0 28-29 36.583220490561985 40.0 38.0 41.0 29.5 41.0 30-31 36.50525643013009 40.0 38.0 41.0 29.0 41.0 32-33 36.40995957692904 40.0 37.0 41.0 29.0 41.0 34-35 36.3013605523294 40.0 37.0 41.0 28.5 41.0 36-37 36.19968830115784 40.0 37.0 41.0 28.0 41.0 38-39 36.09821828458319 40.0 37.0 41.0 27.0 41.0 40-41 35.98231663546498 40.0 37.0 41.0 27.0 41.0 42-43 35.816262952672815 40.0 36.0 41.0 26.0 41.0 44-45 35.616635226500364 40.0 36.0 41.0 26.0 41.0 46-47 35.40575152331779 40.0 35.0 41.0 24.5 41.0 48-49 35.200269487139735 40.0 35.0 41.0 24.0 41.0 50-51 35.03240165508031 39.5 35.0 41.0 24.0 41.0 52-53 34.847379893397566 39.0 35.0 41.0 23.0 41.0 54-55 34.63945792542599 39.0 35.0 41.0 23.0 41.0 56-57 34.402425145773464 39.0 35.0 41.0 22.0 41.0 58-59 34.14015429927382 38.0 34.0 40.0 21.5 41.0 60-61 33.85802177360695 38.0 34.0 40.0 20.0 41.0 62-63 33.532177479937516 37.0 34.0 40.0 19.0 41.0 64-65 33.19492767966803 36.5 34.0 39.5 18.0 41.0 66-67 32.863354041710885 36.0 33.0 39.0 16.5 41.0 68-69 32.51114245853356 35.5 33.0 39.0 13.5 40.5 70-71 32.441051953781766 35.0 33.0 39.0 13.5 40.0 72-73 32.14721724717694 35.0 33.0 37.5 12.0 40.0 74-75 31.821834420423784 35.0 33.0 37.0 9.0 39.0 76-77 31.520525619164587 35.0 33.0 36.5 7.0 39.0 78-79 31.235233177921135 35.0 33.0 36.0 6.5 38.0 80-81 30.966761265397135 35.0 33.0 36.0 2.0 37.0 82-83 30.745600801306892 35.0 32.5 35.0 2.0 37.0 84-85 30.536365977844817 35.0 32.0 35.0 2.0 36.0 86-87 30.375042867533956 35.0 32.0 35.0 2.0 36.0 88-89 30.241166903163496 35.0 32.0 35.0 2.0 36.0 90-91 30.119662783349035 35.0 32.0 35.0 2.0 35.5 92-93 30.01061087726411 35.0 32.0 35.0 2.0 35.0 94-95 29.890933784863407 35.0 32.0 35.0 2.0 35.0 96-97 29.808236767108262 35.0 31.5 35.0 2.0 35.0 98-99 29.74176454455481 35.0 31.0 35.0 2.0 35.0 100-101 29.661500542551543 35.0 31.0 35.0 2.0 35.0 102-103 28.884529291821185 34.0 29.5 35.0 2.0 35.0 104-105 29.448892837127218 34.5 31.0 35.0 2.0 35.0 106-107 29.524567687776493 35.0 31.0 35.0 2.0 35.0 108-109 29.47105827361292 35.0 31.0 35.0 2.0 35.0 110-111 29.39854238460346 35.0 31.0 35.0 2.0 35.0 112-113 29.31734185516855 35.0 31.0 35.0 2.0 35.0 114-115 29.19648593539463 35.0 31.0 35.0 2.0 35.0 116-117 29.07236492851436 34.0 31.0 35.0 2.0 35.0 118-119 29.075839404743448 34.5 31.0 35.0 2.0 35.0 120-121 29.238894387274485 35.0 31.5 35.0 2.0 35.0 122-123 29.113964680490803 35.0 31.0 35.0 2.0 35.0 124-125 28.571850756591108 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85042.0 3 11296.0 4 7141.0 5 6202.0 6 6739.0 7 7494.0 8 7526.0 9 7807.0 10 8732.0 11 9154.0 12 9326.0 13 9347.0 14 9975.0 15 8752.0 16 6509.0 17 5870.0 18 5582.0 19 5321.0 20 5244.0 21 5508.0 22 6313.0 23 8576.0 24 12410.0 25 15585.0 26 15998.0 27 17273.0 28 20317.0 29 24478.0 30 29780.0 31 36926.0 32 47960.0 33 66357.0 34 107656.0 35 177781.0 36 377823.0 37 691435.0 38 211219.0 39 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03487956615096 17.560715442562373 12.385430297398212 25.018974693888456 2 16.037934758550158 19.929629601361615 39.770628372550966 24.261807267537264 3 19.104253196898004 25.757025734033164 28.295701676597517 26.84301939247132 4 13.503198021653432 15.510882051008062 35.426151650339456 35.55976827699905 5 14.126127174688705 39.11949673534339 32.21773408789959 14.536642002068314 6 32.922715639891834 34.90258346104007 17.691278600423292 14.483422298644813 7 29.443094445541618 33.03762155953018 20.014186914265053 17.505097080663152 8 27.58448464865205 35.52887041823252 19.142173488692922 17.7444714444225 9 28.89598681160167 16.153164395140593 18.008164519163437 36.9426842740943 10-11 26.544822725651983 26.156203677976148 26.283875076172336 21.015098520199533 12-13 26.385702912622282 23.574634365995095 26.75573726154071 23.28392545984191 14-15 24.056351773818257 24.49831571455965 24.141543894930354 27.303788616691744 16-17 24.112961311156372 27.50234918696666 24.63851140250039 23.74617809937657 18-19 24.32779481592481 26.9656481858885 26.063428016882913 22.64312898130378 20-21 25.48274325814613 25.708290562740167 25.630134856157248 23.178831322956455 22-23 25.20758901953302 25.130911779436666 25.621693815736123 24.039805385294187 24-25 24.54421651891655 25.390792508269953 25.64493702434359 24.42005394846991 26-27 24.19312563335646 25.693529049671366 25.77423616227186 24.33910915470032 28-29 24.267133489232354 26.247750653786117 25.450018304818762 24.03509755216277 30-31 25.04734781858982 25.697946555933626 25.47189813654345 23.7828074889331 32-33 24.61283666014438 25.67479901278476 25.48249333858678 24.229870988484077 34-35 24.552006033196893 25.866520765826767 25.855167975974062 23.726305225002278 36-37 24.47611222478347 25.99866200315538 25.616700871844465 23.90852490021668 38-39 24.652621426281808 26.001089478424127 25.520411979301816 23.825877115992245 40-41 24.571022707623342 25.80207931604998 25.51008020614283 24.116817770183847 42-43 24.238934889255283 25.849799694288432 25.442534071766616 24.468731344689676 44-45 24.112662915179403 25.818099773279407 25.76911133445557 24.30012597708562 46-47 24.076706053068488 25.60623345202282 25.64351223891433 24.67354825599436 48-49 24.014087043254502 25.94785952739334 25.671933407571906 24.36612002178025 50-51 24.218880801661097 25.888085890079736 25.66888168410649 24.22415162415267 52-53 24.33250351903906 25.901630038927653 25.540233909369782 24.225632532663504 54-55 23.87871713830793 25.48174570390348 25.967808253809554 24.671728903979034 56-57 24.087762371023622 25.666317339491158 25.492186408104132 24.753733881381084 58-59 24.257022767693492 25.878625566950415 25.626623755284918 24.23772791007118 60-61 24.82778078814914 25.75236494350466 25.513104019354564 23.90675024899163 62-63 24.45000463879526 25.744216590955325 25.684782772015257 24.120995998234157 64-65 24.179206068805907 25.40890339884517 25.80408744800539 24.607803084343534 66-67 24.259217169338246 25.8070247698346 25.498674803474298 24.435083257352854 68-69 24.21922681470871 25.71994362801742 25.617507655270394 24.443321902003476 70-71 24.140969814396914 25.552370826482505 25.774459172425175 24.532200186695402 72-73 24.201445664057207 26.17909768583089 25.277537162361984 24.34191948774992 74-75 24.053014538883364 25.929157972536366 25.52543392345699 24.49239356512328 76-77 23.935650849118208 25.365936725036242 25.737013044314033 24.961399381531518 78-79 23.991086093748173 25.520659556560833 25.838379641874464 24.64987470781653 80-81 24.331009218947315 25.926622855500664 25.28929798389934 24.453069941652686 82-83 24.37385267438813 25.840449922166098 25.51609231122208 24.269605092223692 84-85 24.287851316110203 25.944980053587948 25.21788885247669 24.549279777825163 86-87 23.8954684902194 26.187899803498393 25.388751922622394 24.527879783659813 88-89 23.99107069391727 25.624031853582274 25.641728288179998 24.74316916432046 90-91 24.376780388961922 25.36530767220046 25.638769692795254 24.61914224604236 92-93 24.53137454403913 25.62153065473763 25.43263513542521 24.414459665798034 94-95 24.38529702316188 25.57131947382656 25.4489202778643 24.594463225147262 96-97 23.88648102531421 25.641059888225943 25.615229548954098 24.85722953750575 98-99 23.758754793283607 25.6184224271533 25.476585593651663 25.14623718591143 100-101 23.799378265430192 25.821995207959574 25.60228449532125 24.77634203128898 102-103 23.823721262465998 25.756223906814014 25.651189330547673 24.768865500172314 104-105 23.93626789502128 25.791106258493475 25.525849967302072 24.74677587918318 106-107 23.875936600248423 25.624829470543002 25.67885034048651 24.820383588722063 108-109 23.90925597368281 25.56445509962319 25.76045548027129 24.765833446422707 110-111 23.70213717940401 26.28445894119847 25.511207742004423 24.502196137393103 112-113 23.63675260457472 26.334377238152683 25.46815327108064 24.560716886191955 114-115 23.842722879291166 26.360638316139728 25.375408534921746 24.421230269647356 116-117 23.60740586338857 26.125005425706927 25.548258741588963 24.719329969315538 118-119 23.595145460160666 25.81805292452562 25.761946401909487 24.824855213404227 120-121 23.593062080502897 25.98156448791927 25.856727540380604 24.56864589119723 122-123 23.626881269606866 26.124206603027964 25.581593905719135 24.66731822164603 124-125 23.574042924727305 25.993230243967353 25.53375684255488 24.898969988750462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 770.0 1 670.0 2 523.0 3 413.0 4 305.0 5 254.5 6 291.5 7 398.5 8 536.5 9 588.0 10 521.5 11 508.0 12 573.0 13 605.5 14 671.0 15 757.5 16 819.0 17 874.5 18 954.5 19 1057.0 20 1169.0 21 1271.5 22 1419.0 23 1663.5 24 1900.5 25 2315.0 26 2996.5 27 3889.0 28 5064.0 29 6770.0 30 9069.0 31 11861.5 32 15647.0 33 19702.0 34 23677.5 35 28002.0 36 32981.5 37 37545.0 38 41488.0 39 45863.0 40 49934.0 41 53500.0 42 57017.5 43 62718.5 44 69203.5 45 74690.5 46 79093.0 47 83514.0 48 88843.0 49 92608.0 50 94234.0 51 94789.0 52 94275.0 53 92547.5 54 90203.5 55 86599.5 56 81222.0 57 74154.0 58 67568.5 59 60205.0 60 51208.5 61 43345.5 62 35688.5 63 27319.0 64 20381.0 65 15476.5 66 11494.5 67 8554.0 68 6510.5 69 5139.5 70 4174.5 71 3295.0 72 2589.0 73 2165.0 74 1690.0 75 1351.5 76 1037.0 77 627.5 78 456.5 79 343.0 80 226.5 81 155.5 82 102.0 83 71.5 84 54.5 85 36.5 86 28.0 87 22.5 88 15.0 89 10.5 90 11.5 91 13.5 92 14.0 93 9.5 94 7.5 95 8.0 96 6.5 97 4.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01726625567890488 2 0.01139954449518858 3 0.011733422367432599 4 0.013307418050868679 5 0.008728521517236446 6 0.029905917985285527 7 0.013212024373084674 8 0.013212024373084674 9 0.008919308872804455 10-11 0.00846618890333043 12-13 0.0070591321560163605 14-15 0.006534466928204334 16-17 0.005008168083660255 18-19 0.006462921669866329 20-21 0.005675923828148289 22-23 0.005962104861500304 24-25 0.0062005890559603165 26-27 0.005032016503106257 28-29 0.006319831153190322 30-31 0.005795165925378295 32-33 0.008251553128316422 34-35 0.008299249967208423 36-37 0.0075122521254903825 38-39 0.007583797383828387 40-41 0.007846129997734399 42-43 0.007369161608814376 44-45 0.007726887900504394 46-47 0.009944790908982508 48-49 0.008609279420006439 50-51 0.006105195378176312 52-53 0.005819014344824297 54-55 0.006534466928204334 56-57 0.007822281578288398 58-59 0.007703039481058393 60-61 0.005675923828148289 62-63 0.005985953280946305 64-65 0.003982686047482203 66-67 0.004459654436402227 68-69 0.006844496381002349 70-71 0.0053420459559042725 72-73 0.004721987050308241 74-75 0.004459654436402227 76-77 0.005055864922552258 78-79 0.0036011113363461837 80-81 0.0036965050141301884 82-83 0.0058905596031623006 84-85 0.009920942489536505 86-87 0.007369161608814376 88-89 0.005127410180890262 90-91 0.005866711183716299 92-93 0.008490037322776433 94-95 0.0074407068671523795 96-97 0.009682458295076494 98-99 0.00724991951158437 100-101 0.008680824678344443 102-103 0.005842862764270298 104-105 0.0053420459559042725 106-107 0.007774584739396397 108-109 0.007178374253246366 110-111 0.007965372094964406 112-113 0.00569977224759429 114-115 0.0028141134946281436 116-117 0.0035295660780081803 118-119 0.15432312223507386 120-121 0.011471089753526585 122-123 0.0024802356223841263 124-125 0.0021225093306941082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2096575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.03989346990438 #Duplication Level Percentage of deduplicated Percentage of total 1 79.78939115281037 47.10757153690455 2 11.052886912112417 13.051225316520352 3 3.5967742570031693 6.370595069062849 4 1.6490751496752027 3.894448846427624 5 0.9315004979865464 2.7497845084144292 6 0.5915448926529452 2.095484846693754 7 0.4222971714982209 1.745266601252783 8 0.3183560785911014 1.5036567172412105 9 0.24251964524148031 1.2886500617474401 >10 1.3021429802968543 13.858700392638285 >50 0.0751624273876242 3.0318934807467555 >100 0.027138344957132465 2.6895817935203645 >500 0.0010490911486879101 0.44605054312710934 >1k 1.6139863825966738E-4 0.1670902857024883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.769683889200243E-5 2 0.0 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 3 0.0 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 4 0.0 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 5 4.769683889200243E-5 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 6 4.769683889200243E-5 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 7 4.769683889200243E-5 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 8 4.769683889200243E-5 0.0 0.0 4.769683889200243E-5 4.769683889200243E-5 9 4.769683889200243E-5 0.0 0.0 4.769683889200243E-5 9.539367778400486E-5 10-11 4.769683889200243E-5 0.0 0.0 7.154525833800365E-5 9.539367778400486E-5 12-13 4.769683889200243E-5 0.0 0.0 9.539367778400486E-5 9.539367778400486E-5 14-15 4.769683889200243E-5 0.0 0.0 9.539367778400486E-5 9.539367778400486E-5 16-17 4.769683889200243E-5 0.0 0.0 9.539367778400486E-5 9.539367778400486E-5 18-19 4.769683889200243E-5 0.0 0.0 1.1924209723000609E-4 1.430905166760073E-4 20-21 9.539367778400486E-5 0.0 0.0 1.430905166760073E-4 1.9078735556800973E-4 22-23 9.539367778400486E-5 0.0 0.0 2.6233261390601337E-4 2.6233261390601337E-4 24-25 9.539367778400486E-5 0.0 0.0 5.48513647258028E-4 2.861810333520146E-4 26-27 9.539367778400486E-5 0.0 0.0 0.0011924209723000608 2.861810333520146E-4 28-29 9.539367778400486E-5 0.0 0.0 0.003004900850196153 2.861810333520146E-4 30-31 9.539367778400486E-5 0.0 0.0 0.013235872792530674 2.861810333520146E-4 32-33 9.539367778400486E-5 0.0 0.0 0.037370473271883906 3.8157471113601946E-4 34-35 9.539367778400486E-5 0.0 0.0 0.06720484599883142 3.8157471113601946E-4 36-37 9.539367778400486E-5 0.0 0.0 0.10927345790157758 3.8157471113601946E-4 38-39 9.539367778400486E-5 0.0 0.0 0.18348973921753337 4.054231305820207E-4 40-41 9.539367778400486E-5 0.0 0.0 0.2700595018065178 4.292715500280219E-4 42-43 9.539367778400486E-5 0.0 0.0 0.34976091959505384 4.292715500280219E-4 44-45 9.539367778400486E-5 0.0 0.0 0.4349236254367242 4.292715500280219E-4 46-47 9.539367778400486E-5 0.0 0.0 0.5276224318233309 4.5311996947402307E-4 48-49 9.539367778400486E-5 0.0 0.0 0.6301706354411362 4.769683889200243E-4 50-51 9.539367778400486E-5 0.0 0.0 0.7404218785399997 4.769683889200243E-4 52-53 9.539367778400486E-5 0.0 0.0 0.8526048436139895 6.200589055960316E-4 54-55 9.539367778400486E-5 0.0 0.0 0.9691997662854894 6.677557444880341E-4 56-57 9.539367778400486E-5 0.0 0.0 1.0882033793210355 7.154525833800364E-4 58-59 9.539367778400486E-5 0.0 0.0 1.2249263680049605 7.154525833800364E-4 60-61 9.539367778400486E-5 0.0 0.0 1.371904177050666 7.869978417180401E-4 62-63 9.539367778400486E-5 0.0 0.0 1.528922170683138 8.585431000560438E-4 64-65 9.539367778400486E-5 0.0 0.0 1.6765200386344397 8.585431000560438E-4 66-67 9.539367778400486E-5 0.0 0.0 1.8341342427530616 9.300883583940474E-4 68-69 9.539367778400486E-5 0.0 0.0 2.015739956834361 0.001001633616732051 70-71 9.539367778400486E-5 0.0 0.0 2.2147550171112407 0.0010254820361780522 72-73 9.539367778400486E-5 0.0 0.0 2.411385235443521 0.0011447241334080582 74-75 9.539367778400486E-5 0.0 0.0 2.607490788547989 0.0011924209723000608 76-77 9.539367778400486E-5 0.0 0.0 2.8284702431346362 0.0011924209723000608 78-79 9.539367778400486E-5 0.0 0.0 3.075611413853547 0.0011924209723000608 80-81 9.539367778400486E-5 0.0 0.0 3.3260675148754517 0.0011924209723000608 82-83 9.539367778400486E-5 0.0 0.0 3.5918581496011353 0.0011924209723000608 84-85 9.539367778400486E-5 0.0 0.0 3.865208733291201 0.0011924209723000608 86-87 9.539367778400486E-5 0.0 0.0 4.157900385151974 0.0011924209723000608 88-89 9.539367778400486E-5 0.0 0.0 4.470934738800186 0.0012401178111920632 90-91 9.539367778400486E-5 0.0 0.0 4.812754134719722 0.0012401178111920632 92-93 9.539367778400486E-5 0.0 0.0 5.160702574436879 0.0013593599084220694 94-95 9.539367778400486E-5 0.0 0.0 5.516282508376758 0.0013832083278680705 96-97 9.539367778400486E-5 0.0 0.0 5.8964740111849085 0.0015024504250980765 98-99 9.539367778400486E-5 0.0 0.0 6.309981755959123 0.0015739956834360802 100-101 9.539367778400486E-5 0.0 0.0 6.770351644944731 0.0015739956834360802 102-103 9.539367778400486E-5 0.0 0.0 7.24932330109822 0.0015978441028820815 104-105 9.539367778400486E-5 0.0 0.0 7.749114627428067 0.0016216925223280828 106-107 9.539367778400486E-5 0.0 0.0 8.27750020867367 0.0016216925223280828 108-109 9.539367778400486E-5 0.0 0.0 8.82098183942859 0.0016216925223280828 110-111 9.539367778400486E-5 0.0 0.0 9.387882618079487 0.0016693893612200852 112-113 9.539367778400486E-5 0.0 0.0 9.984784708393452 0.0017170862001120875 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2110 0.0 62.330814 1 GTATCAA 4275 0.0 59.580017 1 TCAACGC 5470 0.0 45.690285 4 CAACGCA 5695 0.0 43.884087 5 GTACATG 13490 0.0 43.45275 1 ATCAACG 5820 0.0 43.044827 3 TACATGG 13955 0.0 41.618065 2 ACATGGG 13930 0.0 41.521885 3 AACGCAG 6090 0.0 41.23218 6 TATCAAC 6245 0.0 40.496582 2 CATGGGG 11995 0.0 39.48887 4 ATGGGGG 7880 0.0 37.454758 5 ACGCAGA 7375 0.0 33.960026 7 CGCAGAG 7870 0.0 31.748447 8 TGGGGGG 5805 0.0 31.673601 6 GCAGAGT 8945 0.0 27.93229 9 GTATAGG 630 0.0 27.393682 1 TATACCG 225 6.552E-9 26.446548 5 ATGGGGA 3355 0.0 25.00795 5 AGAGTAC 8055 0.0 24.519123 10-11 >>END_MODULE