FastQCFastQC Report
Mon 15 Aug 2016
SRR2921348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096575
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31070.14819407843745155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.12482262738037038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.11385235443520982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.071.1581
GTATCAA52350.057.8710981
GTACATG122300.041.5300871
TACATGG124800.040.4421422
ACATGGG127500.039.3052983
TCAACGC75050.038.7466744
ATCAACG76650.038.2488373
CAACGCA76800.037.7861825
TATCAAC81950.036.9385642
AACGCAG79900.036.7676126
CATGGGG109800.036.198354
ATGGGGG67850.033.8124125
ACGCAGA94650.030.275137
TGGGGGG49200.028.7044056
CGCAGAG100350.028.430628
GTGGTAT11500.027.5390191
GTAAGTA12000.027.3115752
AGAGTAC104550.025.4936510-11
GCAGAGT113250.024.403279
TATACCG2201.5178921E-724.3772745
Produced by FastQC (version 0.11.5)