FastQCFastQC Report
Mon 15 Aug 2016
SRR2921347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296124
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.061.7422831
GGTATCA17550.054.5947881
TCAACGC43650.046.214694
CAACGCA44050.045.7940335
AACGCAG46750.042.8918766
TATCAAC48650.041.709612
ATCAACG49950.040.5049443
GTACATG115250.040.070361
TACATGG120200.038.0703162
ACATGGG125650.036.4663933
ACGCAGA59400.033.8510257
CATGGGG114850.033.833494
CGCAGAG64600.031.1261778
ATGGGGG70800.030.6771285
GCAGAGT74300.026.261939
ATGGGGA41550.023.7734495
AGAGTAC65000.023.6583310-11
AGTACAT80400.021.6858352
TGGGGGG51300.021.5735866
GAGTACA81650.021.5014741
Produced by FastQC (version 0.11.5)