##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921347_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2296124
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78373249876749	31.0	31.0	34.0	28.0	34.0
2	30.778162242108877	33.0	31.0	34.0	27.0	34.0
3	30.965681295957882	33.0	31.0	34.0	27.0	34.0
4	34.41802533312661	37.0	35.0	37.0	32.0	37.0
5	34.43750337525325	37.0	35.0	37.0	32.0	37.0
6	34.460890178405	37.0	35.0	37.0	32.0	37.0
7	34.46535683612906	37.0	35.0	37.0	32.0	37.0
8	34.43816753799011	37.0	35.0	37.0	31.0	37.0
9	36.08147382284232	39.0	37.0	39.0	32.0	39.0
10-11	36.08118507537049	39.0	37.0	39.0	32.0	39.0
12-13	36.00453416278912	39.0	37.0	39.0	32.0	39.0
14-15	37.34740632474553	41.0	38.0	41.0	31.5	41.0
16-17	37.33615257712563	41.0	38.0	41.0	31.0	41.0
18-19	37.322100200163405	41.0	38.0	41.0	31.0	41.0
20-21	37.34676763972678	41.0	38.0	41.0	31.0	41.0
22-23	37.29548992127603	41.0	38.0	41.0	31.0	41.0
24-25	37.26574414099587	41.0	38.0	41.0	31.0	41.0
26-27	37.22467384165664	41.0	38.0	41.0	31.0	41.0
28-29	37.158971161836206	41.0	38.0	41.0	30.5	41.0
30-31	37.08582768178025	40.0	38.0	41.0	30.0	41.0
32-33	37.01282618011919	40.0	38.0	41.0	30.0	41.0
34-35	36.899488660020104	40.0	38.0	41.0	30.0	41.0
36-37	36.79035735874892	40.0	38.0	41.0	30.0	41.0
38-39	36.68864638843547	40.0	37.5	41.0	30.0	41.0
40-41	36.585136299259105	40.0	37.0	41.0	30.0	41.0
42-43	36.393853729154	40.0	37.0	41.0	29.0	41.0
44-45	36.13311084244579	40.0	36.0	41.0	28.5	41.0
46-47	35.91386789215217	40.0	36.0	41.0	28.0	41.0
48-49	35.70600128738692	40.0	35.0	41.0	27.5	41.0
50-51	35.52555545780629	39.0	35.0	41.0	27.0	41.0
52-53	35.32333205872157	39.0	35.0	41.0	27.0	41.0
54-55	35.1070667350718	39.0	35.0	41.0	26.0	41.0
56-57	34.84973655603966	38.5	35.0	40.0	26.0	41.0
58-59	34.56415616055579	38.0	34.5	40.0	26.0	41.0
60-61	34.263902777027724	37.5	34.0	40.0	25.5	41.0
62-63	33.933828486614836	37.0	34.0	40.0	25.0	41.0
64-65	33.57404739465291	36.5	34.0	39.0	23.5	41.0
66-67	33.22631900541957	36.0	33.0	39.0	23.0	41.0
68-69	32.87188692770948	35.0	33.0	38.5	22.5	40.0
70-71	32.82193252629213	35.0	33.0	38.0	23.5	40.0
72-73	32.53547826685318	35.0	33.0	37.0	23.0	39.5
74-75	32.213957956974454	35.0	33.0	37.0	22.0	39.0
76-77	31.924943949020175	35.0	33.0	36.0	22.0	39.0
78-79	31.64806234332292	35.0	33.0	36.0	20.5	37.5
80-81	31.385259898855637	35.0	33.0	35.5	20.0	37.0
82-83	31.179012326860395	35.0	33.0	35.0	20.0	37.0
84-85	30.980366260707175	35.0	33.0	35.0	19.0	36.0
86-87	30.825656846058834	35.0	32.0	35.0	18.0	36.0
88-89	30.694400868594208	35.0	32.0	35.0	18.0	36.0
90-91	30.58336810207114	35.0	32.0	35.0	17.5	35.0
92-93	30.480347969012126	35.0	32.0	35.0	16.0	35.0
94-95	30.366926176460854	35.0	32.0	35.0	14.0	35.0
96-97	30.284257296208743	35.0	32.0	35.0	10.5	35.0
98-99	30.21622569164383	35.0	32.0	35.0	9.0	35.0
100-101	30.13036578163897	35.0	32.0	35.0	7.0	35.0
102-103	29.271433293672295	33.5	30.0	34.5	7.0	35.0
104-105	29.89682395201653	34.0	31.0	35.0	7.0	35.0
106-107	29.990964120404648	35.0	31.0	35.0	3.5	35.0
108-109	29.93536977968089	35.0	31.0	35.0	2.0	35.0
110-111	29.866416622098807	35.0	31.0	35.0	2.0	35.0
112-113	29.785512672660538	34.0	31.0	35.0	2.0	35.0
114-115	29.666293066053925	34.0	31.0	35.0	2.0	35.0
116-117	29.53769003764605	34.0	31.0	35.0	2.0	35.0
118-119	29.538138401932997	34.5	31.0	35.0	2.0	35.0
120-121	29.72363709451232	35.0	32.0	35.0	2.0	35.0
122-123	29.598109466213497	35.0	32.0	35.0	2.0	35.0
124-125	29.012534819548073	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68668.0
3	10814.0
4	6722.0
5	5952.0
6	6310.0
7	6885.0
8	7201.0
9	7608.0
10	8234.0
11	8822.0
12	8993.0
13	8526.0
14	8194.0
15	7467.0
16	6583.0
17	6030.0
18	5913.0
19	5767.0
20	6010.0
21	6090.0
22	6806.0
23	9099.0
24	12676.0
25	15714.0
26	17379.0
27	19726.0
28	23876.0
29	28770.0
30	35001.0
31	44057.0
32	57136.0
33	80103.0
34	127505.0
35	214924.0
36	458043.0
37	748219.0
38	190245.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.66390180305639	17.46407325305907	12.236677668332236	25.6353472755523
2	16.14960507139379	19.574860466108966	39.223113973076735	25.052420489420513
3	19.372678699065315	24.176810663730365	28.389137476767935	28.061373160436386
4	13.745736798776903	15.173860206201734	35.08038557197678	36.00001742304459
5	14.782380312111673	37.59269204485021	32.68052000318828	14.944407639849839
6	33.85729558109972	33.977750845365065	17.335756088763112	14.829197484772102
7	29.964326316202122	31.59207425701604	20.354079823678788	18.089519603103042
8	27.462883551157212	35.044679122664505	19.21004087872934	18.282396447448946
9	28.34703011782661	15.72683843834008	18.158852501572355	37.76727894226095
10-11	26.73826860876663	25.259349789671653	26.543445935292564	21.458935666269152
12-13	26.644086458691525	22.563882221995254	26.775665205479093	24.016366113834124
14-15	24.243018826178034	23.72920739458144	23.885197531248657	28.14257624799187
16-17	24.683299521171	26.66810538941768	24.435217842368832	24.213377247042487
18-19	24.758697495663064	26.36502891797261	25.50452378225043	23.371749804113893
20-21	25.57494124008518	25.277336127183176	25.220541590782737	23.927181041948906
22-23	25.633945870562087	24.70676675683086	25.12667157811434	24.532615794492717
24-25	25.05492193767732	25.00095819399596	25.126568716011334	24.81755115231539
26-27	24.65516325248888	25.274083694611623	25.33068178155708	24.740071271342423
28-29	24.789033532943	25.651606001966044	25.07130938801323	24.488051077077728
30-31	25.35914175752599	25.329546738896802	24.889650214754433	24.421661288822776
32-33	25.083440951072223	25.303590911763617	24.928798071373972	24.68417006579019
34-35	24.994370499938807	25.555360525379356	25.15968398142635	24.290584993255486
36-37	25.080478423594172	25.439326607597618	25.118676003078455	24.361518965729758
38-39	25.143491175804456	25.56534085959686	24.976284430042337	24.31488353455635
40-41	25.058510472650507	25.360105907929874	25.055222082822564	24.52616153659706
42-43	24.82608536582179	25.297545159876893	24.988065962994032	24.88830351130728
44-45	24.69322631597401	25.291104799034297	25.290582139910185	24.725086745081505
46-47	24.740145703083147	25.213271298165164	25.187007110930693	24.859575887820995
48-49	24.63147863264243	25.437164515325367	25.190924644109668	24.740432207922535
50-51	24.645398140802413	25.453328312987356	25.266110639395066	24.635162906815165
52-53	24.766185299579202	25.357780535719694	25.157627704409702	24.718406460291405
54-55	24.413517899561754	25.107535873601577	25.44412446460724	25.03482176222943
56-57	24.622606706513693	25.231614667063308	25.113602361030114	25.032176265392884
58-59	24.73714675965327	25.37010694446501	25.221258402810502	24.671487893071216
60-61	25.094697258407116	25.262336558215786	25.26804213064367	24.374924052733427
62-63	24.848191715287708	25.3255461265477	25.27376014160353	24.55250201656106
64-65	24.643690876465566	25.123364575530044	25.283422762669638	24.94952178533475
66-67	24.727888889905138	25.307628954163906	25.162182184981113	24.802299970949846
68-69	24.5711944967946	25.21299267281686	25.311208199058267	24.904604631330272
70-71	24.575012554393297	25.061095143790812	25.446807917564783	24.917084384251105
72-73	24.71073294923364	25.515430298144377	25.028151994419908	24.74568475820208
74-75	24.479487532940002	25.495589017654602	25.221942826979	24.8029806224264
76-77	24.519564436738342	25.027727382502423	25.245517724074595	25.207190456684636
78-79	24.442455521438994	25.16569761111474	25.3343568302084	25.057490037237866
80-81	24.775581684521267	25.390638780477925	25.004257317032273	24.829522217968535
82-83	24.753064521713792	25.320639923135708	25.22351425493397	24.70278130021653
84-85	24.66144084296134	25.41417200878433	25.01476541286969	24.909621735384636
86-87	24.348921016847868	25.618216883758326	25.235304827004672	24.797557272389128
88-89	24.387932894906186	25.260141449701557	25.277475865201836	25.074449790190418
90-91	24.642912051944183	25.02152653981724	25.36346860173963	24.972092806498946
92-93	24.80410447964344	25.156750171662427	25.23430042851907	24.804844920175068
94-95	24.747671073752763	25.182216687866937	25.203427844935554	24.866684393444753
96-97	24.42843282761354	25.034730351136425	25.410530281664833	25.126306539585197
98-99	24.265601956826814	25.08310242068466	25.3028142451835	25.348481377305028
100-101	24.275907787117752	25.276549575955503	25.420369510957773	25.02717312596897
102-103	24.235990777860504	25.107529562871722	25.48028709974079	25.17619255952698
104-105	24.276167276493496	25.250249672583752	25.312531712664637	25.161051338258115
106-107	24.34236372743309	25.016420218210328	25.456586598140202	25.184629456216378
108-109	24.311426824883604	25.047741436430883	25.478911558403745	25.161920180281765
110-111	24.099780482068347	25.54552819324547	25.35425577323449	25.000435551451694
112-113	24.126432028216023	25.50010333185903	25.400734886599547	24.9727297533254
114-115	24.17867616523839	25.669836365659588	25.21089890985205	24.940588559249967
116-117	23.998153348308342	25.530939582042617	25.38889112703461	25.082015942614426
118-119	24.110731334829417	25.25320822018019	25.46763120293889	25.16842924205151
120-121	24.027700162596243	25.368672717238905	25.640922334050998	24.962704786113854
122-123	24.08638575515674	25.497468939358637	25.40707547190355	25.009069833581076
124-125	24.062603233032654	25.41719483145074	25.308204702842392	25.211997232674214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	474.0
1	385.5
2	279.5
3	234.0
4	189.5
5	166.0
6	164.5
7	192.0
8	212.0
9	219.0
10	226.5
11	243.0
12	275.0
13	290.5
14	326.0
15	371.0
16	418.0
17	486.5
18	523.0
19	541.0
20	621.5
21	738.0
22	867.0
23	1052.0
24	1284.0
25	1598.0
26	2155.0
27	2986.0
28	4006.0
29	5535.0
30	7778.0
31	10553.0
32	14208.5
33	17923.0
34	21778.0
35	26331.0
36	31304.0
37	36373.0
38	40896.5
39	45592.5
40	50107.5
41	54558.0
42	59220.5
43	65254.5
44	71673.0
45	77221.5
46	83107.0
47	88868.0
48	94847.0
49	100262.0
50	103323.5
51	105660.5
52	105985.5
53	105746.0
54	106205.5
55	104350.5
56	99378.0
57	90936.5
58	82178.5
59	73293.5
60	62664.0
61	52626.0
62	43732.0
63	33927.5
64	25074.5
65	18920.0
66	14244.5
67	10760.5
68	8206.0
69	6121.0
70	4636.0
71	3568.5
72	2669.0
73	2048.0
74	1589.0
75	1165.5
76	822.0
77	503.0
78	333.0
79	226.5
80	142.5
81	94.0
82	71.0
83	61.5
84	44.0
85	32.0
86	22.0
87	18.0
88	13.5
89	11.0
90	10.0
91	6.0
92	3.0
93	1.5
94	2.0
95	3.5
96	2.5
97	3.0
98	3.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01789973015394639
2	0.011584740196958003
3	0.011628291851833785
4	0.013675219630995538
5	0.009363605798293123
6	0.029005402147270792
7	0.013675219630995538
8	0.013457461356616629
9	0.00905874421416265
10-11	0.008274814426398575
12-13	0.006990040607563006
14-15	0.006336765784426277
16-17	0.004616475416832889
18-19	0.006402093266739951
20-21	0.005596387651537983
22-23	0.005552835996662201
24-25	0.006010128372857912
26-27	0.004790682036336017
28-29	0.0062278866472368225
30-31	0.005639939306413765
32-33	0.008231262771522791
34-35	0.0079699528422681
36-37	0.007273126364255589
38-39	0.007142471399628243
40-41	0.00729490219169348
42-43	0.007403781328882935
44-45	0.00751266046607239
46-47	0.009668467382423598
48-49	0.008361917736150137
50-51	0.005814145925916892
52-53	0.005596387651537983
54-55	0.006358541611864168
56-57	0.007708642913013409
58-59	0.0072295747093798065
60-61	0.005356853549721183
62-63	0.005923025063106348
64-65	0.003658339009565685
66-67	0.004137407213199287
68-69	0.006663403195994641
70-71	0.005008440310714927
72-73	0.004594699589394998
74-75	0.004072079730885614
76-77	0.004725354554022344
78-79	0.003222822460807866
80-81	0.0033534774254352115
82-83	0.005639939306413765
84-85	0.009494260762920469
86-87	0.0072295747093798065
88-89	0.004943112828401253
90-91	0.005269750239969619
92-93	0.008427245218463811
94-95	0.00707714391731457
96-97	0.009646691554985706
98-99	0.007055368089876678
100-101	0.00860145183796694
102-103	0.005552835996662201
104-105	0.005095543620466491
106-107	0.007577987948386062
108-109	0.00707714391731457
110-111	0.008013504497143883
112-113	0.005378629377159073
114-115	0.0025913234651090272
116-117	0.0033317015979973203
118-119	0.16081448562882492
120-121	0.011018568683572838
122-123	0.0022211343986648807
124-125	0.0019380486419722975
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2296124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.56514243839952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58226120277531	43.10869394425327
2	11.409148667181524	12.679019415856349
3	4.0758151335819415	6.794197453501948
4	1.9577239969753444	4.351248509880313
5	1.1685326432877763	3.246484138410239
6	0.7706555001473557	2.5692949581974305
7	0.5438318672626243	2.1152666616891946
8	0.39169892524361344	1.741184525930351
9	0.28903749840199966	1.4454368791871204
>10	1.7074757427406835	16.956447827276637
>50	0.08295385710063763	3.077132462499812
>100	0.020473490197655823	1.708293138204979
>500	3.1318008293397634E-4	0.12520664112350186
>1k	7.829502073348015E-5	0.08209344398888657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3065496462734592E-4
7	0.0	0.0	0.0	0.0	1.3065496462734592E-4
8	0.0	0.0	0.0	0.0	1.3065496462734592E-4
9	0.0	0.0	0.0	0.0	2.1775827437890986E-4
10-11	0.0	0.0	0.0	0.0	2.1775827437890986E-4
12-13	0.0	0.0	0.0	0.0	3.048615841304738E-4
14-15	0.0	0.0	0.0	0.0	3.701890664441468E-4
16-17	0.0	0.0	0.0	0.0	3.9196489388203774E-4
18-19	0.0	0.0	0.0	0.0	4.355165487578197E-4
20-21	0.0	0.0	0.0	0.0	4.7906820363360165E-4
22-23	0.0	0.0	0.0	6.532748231367295E-5	5.443956859472746E-4
24-25	0.0	0.0	0.0	3.4841323900625576E-4	6.968264780125115E-4
26-27	0.0	0.0	0.0	7.621539603261845E-4	6.968264780125115E-4
28-29	0.0	4.355165487578197E-5	0.0	0.0031357191510563024	6.968264780125115E-4
30-31	0.0	4.355165487578197E-5	0.0	0.012651755741414663	6.968264780125115E-4
32-33	0.0	4.355165487578197E-5	0.0	0.031030554098994655	7.403781328882934E-4
34-35	0.0	4.355165487578197E-5	0.0	0.054896860970923174	7.403781328882934E-4
36-37	0.0	4.355165487578197E-5	0.0	0.09060921796906439	7.403781328882934E-4
38-39	0.0	4.355165487578197E-5	0.0	0.15160331062259705	7.621539603261845E-4
40-41	0.0	4.355165487578197E-5	0.0	0.22350709282251308	7.839297877640755E-4
42-43	0.0	4.355165487578197E-5	0.0	0.2891829883751923	8.710330975156395E-4
44-45	0.0	4.355165487578197E-5	0.0	0.360564150716599	9.581364072672033E-4
46-47	0.0	4.355165487578197E-5	0.0	0.43394868918229157	9.581364072672033E-4
48-49	0.0	4.355165487578197E-5	0.0	0.5159782311408269	0.0010887913718945492
50-51	0.0	4.355165487578197E-5	0.0	0.6052155719813042	0.0010887913718945492
52-53	0.0	4.355165487578197E-5	0.0	0.6987209749996081	0.001241222163959786
54-55	0.0	4.355165487578197E-5	0.0	0.7930538594605518	0.001350101301149241
56-57	0.0	4.355165487578197E-5	0.0	0.8942461295644313	0.001350101301149241
58-59	0.0	4.355165487578197E-5	0.0	1.0041269548160292	0.001350101301149241
60-61	0.0	4.355165487578197E-5	0.0	1.1218253021178297	0.001393652956025023
62-63	0.0	4.355165487578197E-5	0.0	1.250215580691635	0.001480756265776587
64-65	0.0	4.355165487578197E-5	0.0	1.3772557579642912	0.0015025320932144778
66-67	0.0	4.355165487578197E-5	0.0	1.519277704514216	0.001524307920652369
68-69	0.0	4.355165487578197E-5	0.0	1.6735812177391116	0.001524307920652369
70-71	0.0	4.355165487578197E-5	0.0	1.8430407068607795	0.001567859575528151
72-73	0.0	4.355165487578197E-5	0.0	2.012347765190382	0.0016331870578418237
74-75	0.0	4.355165487578197E-5	0.0	2.184267922812531	0.0016549628852797148
76-77	0.0	4.355165487578197E-5	0.0	2.378617182695708	0.0016549628852797148
78-79	0.0	4.355165487578197E-5	0.0	2.5953955448399126	0.0016549628852797148
80-81	0.0	4.355165487578197E-5	0.0	2.819490585003249	0.0016549628852797148
82-83	0.0	6.532748231367295E-5	0.0	3.056564018319568	0.001676738712717606
84-85	0.0	8.710330975156394E-5	0.0	3.3088152033600973	0.0016985145401554969
86-87	0.0	8.710330975156394E-5	0.0	3.568165308145379	0.001742066195031279
88-89	0.0	8.710330975156394E-5	0.0	3.8620300994197176	0.0017856178499070607
90-91	0.0	8.710330975156394E-5	0.0	4.172618726166357	0.0017856178499070607
92-93	0.0	8.710330975156394E-5	0.0	4.494813868937392	0.0017856178499070607
94-95	0.0	8.710330975156394E-5	0.0	4.826851685710354	0.0018727211596586248
96-97	0.0	8.710330975156394E-5	0.0	5.176527922708007	0.0018727211596586248
98-99	0.0	8.710330975156394E-5	0.0	5.566554767948073	0.0018727211596586248
100-101	0.0	8.710330975156394E-5	0.0	5.989419560964478	0.0018727211596586248
102-103	0.0	8.710330975156394E-5	0.0	6.434865887033975	0.0019162728145344066
104-105	0.0	8.710330975156394E-5	0.0	6.898451477359236	0.0019162728145344066
106-107	0.0	8.710330975156394E-5	0.0	7.392240140340853	0.0019816002968480796
108-109	0.0	8.710330975156394E-5	0.0	7.911288763150422	0.002112255261475426
110-111	0.0	8.710330975156394E-5	0.0	8.450131613101036	0.0022211343986648802
112-113	0.0	8.710330975156394E-5	0.0	9.007418588891541	0.0023082377084164443
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3335	0.0	61.742283	1
GGTATCA	1755	0.0	54.594788	1
TCAACGC	4365	0.0	46.21469	4
CAACGCA	4405	0.0	45.794033	5
AACGCAG	4675	0.0	42.891876	6
TATCAAC	4865	0.0	41.70961	2
ATCAACG	4995	0.0	40.504944	3
GTACATG	11525	0.0	40.07036	1
TACATGG	12020	0.0	38.070316	2
ACATGGG	12565	0.0	36.466393	3
ACGCAGA	5940	0.0	33.851025	7
CATGGGG	11485	0.0	33.83349	4
CGCAGAG	6460	0.0	31.126177	8
ATGGGGG	7080	0.0	30.677128	5
GCAGAGT	7430	0.0	26.26193	9
ATGGGGA	4155	0.0	23.773449	5
AGAGTAC	6500	0.0	23.65833	10-11
AGTACAT	8040	0.0	21.685835	2
TGGGGGG	5130	0.0	21.573586	6
GAGTACA	8165	0.0	21.501474	1
>>END_MODULE