FastQCFastQC Report
Mon 15 Aug 2016
SRR2921347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296124
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.066.093881
GTATCAA41600.057.618831
GTACATG111300.041.3531841
TCAACGC58650.039.6287234
TACATGG119050.038.495542
ACATGGG121700.037.8042143
CAACGCA61450.037.5320785
CATGGGG110150.036.8988954
TATCAAC64750.036.539642
ATCAACG63950.036.3444023
AACGCAG64550.035.624076
ATGGGGG69550.034.36065
TGGGGGG49000.028.570926
ACGCAGA80300.028.3307617
CGCAGAG85600.026.4340368
AGAGTAC85800.023.59534610-11
GCAGAGT96450.023.4603779
GTATACG1552.0425525E-423.1383781
TAAGTAC16950.022.922241
ATGGGGA39300.021.9881615
Produced by FastQC (version 0.11.5)