##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2296124
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.15246781097188	33.0	30.0	34.0	16.0	34.0
2	29.934348057857502	33.0	31.0	34.0	26.0	34.0
3	30.16791253434048	33.0	31.0	34.0	26.0	34.0
4	32.320357262935275	37.0	35.0	37.0	19.0	37.0
5	31.97226020894342	37.0	33.0	37.0	19.0	37.0
6	32.110286291158495	37.0	35.0	37.0	17.0	37.0
7	32.323597506058036	37.0	35.0	37.0	17.0	37.0
8	32.57983758716864	37.0	35.0	37.0	17.0	37.0
9	34.33871472098197	39.0	37.0	39.0	17.0	39.0
10-11	34.519370687297375	39.0	37.0	39.0	21.0	39.0
12-13	34.54617585984032	39.0	37.0	39.0	24.0	39.0
14-15	35.935624992378465	40.5	38.0	41.0	25.0	41.0
16-17	35.9551328673887	41.0	38.0	41.0	25.0	41.0
18-19	35.98279578977442	41.0	38.0	41.0	25.0	41.0
20-21	35.9716254435736	41.0	38.0	41.0	25.0	41.0
22-23	35.92041653673756	41.0	38.0	41.0	24.5	41.0
24-25	35.85780406458885	41.0	38.0	41.0	24.0	41.0
26-27	35.76977049148914	40.0	38.0	41.0	23.5	41.0
28-29	35.69742400671741	40.0	37.0	41.0	23.0	41.0
30-31	35.60203804324156	40.0	37.0	41.0	22.0	41.0
32-33	35.482341110497515	40.0	37.0	41.0	20.5	41.0
34-35	35.39810611273607	40.0	37.0	41.0	19.0	41.0
36-37	35.31597770852097	40.0	36.0	41.0	18.0	41.0
38-39	35.2169619323695	40.0	36.0	41.0	16.5	41.0
40-41	35.089520862113716	40.0	36.0	41.0	15.0	41.0
42-43	34.94852869444333	40.0	35.0	41.0	12.5	41.0
44-45	34.80272559321709	40.0	35.0	41.0	9.0	41.0
46-47	34.598042614423264	40.0	35.0	41.0	7.0	41.0
48-49	34.396205083000744	40.0	35.0	41.0	2.0	41.0
50-51	34.21326940531087	40.0	35.0	41.0	2.0	41.0
52-53	34.02691296288876	39.0	35.0	41.0	2.0	41.0
54-55	33.816112936409354	39.0	35.0	41.0	2.0	41.0
56-57	33.56521990972613	38.5	34.5	41.0	2.0	41.0
58-59	33.48825194109726	38.5	35.0	41.0	2.0	41.0
60-61	33.24140399211889	37.5	34.0	41.0	2.0	41.0
62-63	32.94995414010742	37.0	34.0	40.0	2.0	41.0
64-65	32.646633195768175	36.5	34.0	40.0	2.0	41.0
66-67	32.328463314699036	36.0	34.0	39.0	2.0	41.0
68-69	31.994447599519887	35.5	34.0	39.0	2.0	41.0
70-71	31.668099806456446	35.0	33.0	38.0	2.0	40.5
72-73	31.333269457572847	35.0	33.0	37.0	2.0	39.5
74-75	31.00843007607603	35.0	33.0	37.0	2.0	39.0
76-77	30.72040730378673	35.0	33.0	36.0	2.0	39.0
78-79	30.47669703378389	35.0	33.0	36.0	2.0	37.0
80-81	30.255227287376467	35.0	33.0	35.0	2.0	37.0
82-83	30.066365100491087	35.0	32.0	35.0	2.0	36.5
84-85	29.902384191794518	35.0	32.0	35.0	2.0	36.0
86-87	29.770096040109333	35.0	32.0	35.0	2.0	36.0
88-89	29.652235245134847	35.0	32.0	35.0	2.0	36.0
90-91	29.55764867228425	35.0	32.0	35.0	2.0	35.0
92-93	29.49367564643721	35.0	32.0	35.0	2.0	35.0
94-95	29.42519132241987	35.0	32.0	35.0	2.0	35.0
96-97	29.361845004886497	35.0	32.0	35.0	2.0	35.0
98-99	29.307407831632787	35.0	32.0	35.0	2.0	35.0
100-101	29.256759870111544	35.0	31.5	35.0	2.0	35.0
102-103	28.58139673641319	34.0	30.0	35.0	2.0	35.0
104-105	29.127736785992397	35.0	31.0	35.0	2.0	35.0
106-107	29.189989303713563	35.0	31.0	35.0	2.0	35.0
108-109	29.259962441052835	35.0	31.5	35.0	2.0	35.0
110-111	29.36929843510194	35.0	32.0	35.0	2.0	35.0
112-113	29.333818208424283	35.0	32.0	35.0	2.0	35.0
114-115	29.273351961827842	35.0	32.0	35.0	2.0	35.0
116-117	29.210438983260488	35.0	32.0	35.0	2.0	35.0
118-119	29.129032665483223	35.0	32.0	35.0	2.0	35.0
120-121	29.032516536563357	35.0	32.0	35.0	2.0	35.0
122-123	28.91796610287598	35.0	32.0	35.0	2.0	35.0
124-125	28.39180092190143	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	160188.0
3	9465.0
4	7183.0
5	6658.0
6	6893.0
7	7258.0
8	7454.0
9	7734.0
10	8109.0
11	8304.0
12	8748.0
13	10115.0
14	11411.0
15	9800.0
16	6806.0
17	6109.0
18	5501.0
19	5055.0
20	5029.0
21	5258.0
22	5981.0
23	7258.0
24	9537.0
25	11995.0
26	14659.0
27	17402.0
28	20249.0
29	24763.0
30	29883.0
31	37176.0
32	47656.0
33	64626.0
34	97898.0
35	169498.0
36	385390.0
37	790074.0
38	258707.0
39	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.817516334156416	17.708760352249634	11.807792321307607	27.66593099228635
2	15.571066719393203	19.684781832340065	38.045506253146606	26.698645195120125
3	18.524565746449234	23.82528121303553	28.371333603934286	29.27881943658095
4	13.55454002537809	16.439174258095964	31.435770039478484	38.57051567704746
5	14.708760589324225	37.551771362654115	32.018687209566636	15.720780838455022
6	33.69749308840093	34.06345381701092	16.690057536747485	15.548995557840662
7	29.892066916159642	31.445524438390322	19.291693765739424	19.37071487971061
8	27.895339285356506	34.470498459437074	18.291974350955652	19.342187904250768
9	28.096268688895094	15.604571355896843	18.154018229499	38.14514172570907
10-11	26.785417582635347	25.404011599838245	25.553920149960813	22.256650667565594
12-13	26.7711378409005	22.677379595570013	26.180883840601133	24.370598722928356
14-15	24.238085573775635	23.49738080347577	23.713636545761467	28.55089707698713
16-17	24.359442260087	26.35639451527879	24.282638916713555	25.001524307920654
18-19	24.55161393722639	25.692972156556003	25.75640514188258	23.999008764335027
20-21	25.337917290181196	24.77840917999202	25.609679616606073	24.273993913220714
22-23	25.29536732336755	24.25367706622116	25.394577993174583	25.0563776172367
24-25	24.78017302201449	24.705155296490954	25.298851455757614	25.21582022573694
26-27	24.39084300325244	24.87515918129857	25.667864627520114	25.066133187928873
28-29	24.55076467995631	25.21364264299315	25.36023751330503	24.875355163745514
30-31	25.109902601079035	25.01145408523233	25.203756417336347	24.674886896352287
32-33	24.79815985547819	25.1418912915853	25.246611245734112	24.8133376072024
34-35	24.84441171295627	25.20157883459256	25.530676914661406	24.423332537789772
36-37	24.973019749804454	25.02438892673044	25.568370871956393	24.43422045150872
38-39	24.84428105799164	25.329620699927357	25.38893805386817	24.437160188212832
40-41	24.87485431971444	25.265665094742268	25.254058578717874	24.605422006825414
42-43	24.611519238508027	25.295236668402925	25.118591156226756	24.974652936862295
44-45	24.576612587125084	25.28103882891342	25.38551924896042	24.756829335001072
46-47	24.554074605726868	25.24972518905773	25.09553055497003	25.100669650245372
48-49	24.584234126728347	25.436126271926078	25.047667286261543	24.93197231508403
50-51	24.51627176929469	25.586510136212155	25.141303344244477	24.75591475024868
52-53	24.67693382413145	25.371473840262983	25.111383357344813	24.840208978260755
54-55	24.228547761357834	25.126234471657455	25.424650410866313	25.220567356118394
56-57	24.377124231966565	25.256584574700668	25.12536343855994	25.240927754772823
58-59	24.526332201570995	25.351135217435996	25.188600441439572	24.933932139553438
60-61	24.83158575059535	25.221699699145166	25.324263846377637	24.622450703881846
62-63	24.631879637162452	25.279449193510455	25.293603481345084	24.79506768798201
64-65	24.364842665291594	25.005204422757654	25.385954765509176	25.24399814644157
66-67	24.408307216857626	25.31021843768019	25.201949023658997	25.079525321803175
68-69	24.248058902742187	25.19439281153805	25.32483001789102	25.23271826782874
70-71	24.28242115843918	25.003462356562622	25.432032416367754	25.28208406863044
72-73	24.341760288207432	25.423496292012103	25.05306769146614	25.181675728314325
74-75	24.145102790615837	25.49788251853994	25.16782630206383	25.189188388780398
76-77	24.187021258433777	25.001154118854206	25.154739029773655	25.657085592938362
78-79	24.0907721011583	25.149621710325746	25.301551658359916	25.458054530156037
80-81	24.416799789558404	25.319102975274856	24.963394834076905	25.300702401089836
82-83	24.37387963367832	25.294343859477976	25.124862594528867	25.20691391231484
84-85	24.229484121937666	25.378681639144922	24.92785668369827	25.46397755521914
86-87	23.869638573526515	25.61466628108935	25.101801993272137	25.413893152111992
88-89	23.920441578939116	25.291251691981792	25.13969193301407	25.648614796065022
90-91	24.126579786657214	25.060722079928855	25.2521321866438	25.560565946770136
92-93	24.24079830583214	25.177969285041563	25.13668210943497	25.444550299691326
94-95	24.175419288569877	25.12199376208968	25.078158973730268	25.624427975610175
96-97	23.89008644389748	24.98025467817684	25.24487574329405	25.88478313463163
98-99	23.72686480645854	25.0625728673943	25.149218922505455	26.0613434036417
100-101	23.707365107459353	25.251336600288138	25.30377279275858	25.73752549949393
102-103	23.665517120485895	25.02661551088171	25.2604008451636	26.047466523468792
104-105	23.6002113997788	25.160711085444664	25.15269757920251	26.086379935574023
106-107	23.75563994913158	24.939463094263367	25.25625838370817	26.048638572896888
108-109	23.651203349663117	24.890864402708665	25.31738985672081	26.140542390907413
110-111	23.491886726878544	25.416377409832442	25.227188197101714	25.864547666187303
112-113	23.490603118538658	25.39865838163065	25.208728249875062	25.90201024995563
114-115	23.461157132053188	25.445181026311165	25.117170666209667	25.976491175425988
116-117	23.233113766183088	25.32062400361307	25.287459194545903	26.15880303565794
118-119	23.30776770255342	25.09700618413743	25.307513795576135	26.287712317733007
120-121	23.262367981122942	25.15704206290567	25.426605441656918	26.15398451431447
122-123	23.146329024393324	25.3294856143887	25.254335761095327	26.26984960012264
124-125	22.97344762746761	25.24926261556167	25.084027529019032	26.693262227951685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	49.5
2	79.0
3	94.5
4	82.0
5	103.0
6	207.5
7	502.5
8	778.5
9	846.0
10	752.0
11	665.0
12	688.0
13	770.0
14	896.5
15	937.5
16	953.0
17	1068.5
18	1125.5
19	1196.5
20	1307.0
21	1328.0
22	1495.0
23	1722.0
24	1927.5
25	2262.0
26	2852.0
27	3701.5
28	4538.0
29	5758.5
30	7704.0
31	10061.5
32	13252.5
33	16513.5
34	19801.0
35	23812.0
36	28690.0
37	33845.5
38	38068.5
39	42470.0
40	47217.0
41	51869.5
42	56525.0
43	62254.0
44	68686.0
45	74732.5
46	80899.0
47	86969.0
48	93784.0
49	100037.0
50	104055.0
51	107245.5
52	107605.0
53	106770.0
54	107179.0
55	106222.0
56	100935.5
57	92001.5
58	83751.0
59	74902.5
60	64315.5
61	54290.5
62	45371.5
63	35385.5
64	25814.0
65	19548.0
66	14880.0
67	11333.0
68	8915.0
69	6672.0
70	5002.5
71	3929.0
72	2987.5
73	2367.5
74	1884.5
75	1407.5
76	1036.0
77	720.0
78	509.5
79	399.0
80	330.0
81	257.0
82	218.0
83	197.5
84	187.0
85	182.0
86	166.5
87	146.0
88	138.0
89	133.0
90	131.0
91	114.5
92	107.5
93	110.5
94	98.5
95	84.0
96	68.0
97	58.5
98	38.0
99	15.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3472808959794854
2	0.0
3	0.0
4	0.01929338310997141
5	0.17747299361881153
6	0.04655671906221093
7	0.023300135358543354
8	0.009145847523914214
9	0.007621539603261844
10-11	0.0025042201553574636
12-13	0.055071067590426306
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.048615841304738E-4
92-93	5.008440310714927E-4
94-95	1.0887913718945492E-4
96-97	4.5729237619571064E-4
98-99	4.355165487578197E-5
100-101	0.0
102-103	2.1775827437890985E-5
104-105	2.1775827437890985E-5
106-107	1.7420661950312788E-4
108-109	5.443956859472746E-4
110-111	4.3551654875781967E-4
112-113	7.186023054504024E-4
114-115	3.266374115683648E-4
116-117	6.750506505746206E-4
118-119	3.266374115683648E-4
120-121	1.524307920652369E-4
122-123	0.0019598244694101884
124-125	6.532748231367295E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2296124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.367503667678314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41891473608922	46.93851300809396
2	9.926526595447658	11.587731549341951
3	3.4936365722255163	6.117445363287237
4	1.7522120287902065	4.090889680678497
5	1.0216993857446	2.9817021322356303
6	0.6902656376407145	2.4173449282000026
7	0.45570937139421386	1.8619032884373012
8	0.36426725870740784	1.70090964468958
9	0.2683913943752726	1.409880212601482
>10	1.5146757220174278	15.876093754624202
>50	0.07356315372013085	2.8761033036947623
>100	0.019540339693943053	1.7098649361557843
>500	2.9890207684135455E-4	0.1058546247423296
>1k	2.9890207684068256E-4	0.3257635732175237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.355165487578197E-5	4.355165487578197E-5
5	0.0	0.0	0.0	4.355165487578197E-5	4.355165487578197E-5
6	0.0	0.0	0.0	4.355165487578197E-5	4.355165487578197E-5
7	0.0	0.0	0.0	8.710330975156394E-5	4.355165487578197E-5
8	0.0	0.0	0.0	8.710330975156394E-5	1.3065496462734592E-4
9	0.0	0.0	0.0	8.710330975156394E-5	1.3065496462734592E-4
10-11	0.0	4.355165487578197E-5	0.0	8.710330975156394E-5	1.3065496462734592E-4
12-13	0.0	4.355165487578197E-5	0.0	1.0887913718945493E-4	1.5243079206523691E-4
14-15	0.0	4.355165487578197E-5	0.0	1.3065496462734592E-4	2.1775827437890986E-4
16-17	0.0	4.355165487578197E-5	0.0	1.3065496462734592E-4	3.9196489388203774E-4
18-19	0.0	4.355165487578197E-5	0.0	1.3065496462734592E-4	4.355165487578197E-4
20-21	0.0	4.355165487578197E-5	0.0	1.5243079206523691E-4	4.572923761957107E-4
22-23	0.0	4.355165487578197E-5	0.0	2.3953410181680085E-4	5.443956859472746E-4
24-25	0.0	4.355165487578197E-5	0.0	5.226198585093836E-4	6.097231682609476E-4
26-27	0.0	4.355165487578197E-5	0.0	9.145847523914215E-4	6.314989956988386E-4
28-29	0.0	4.355165487578197E-5	0.0	0.003222822460807866	6.532748231367296E-4
30-31	0.0	4.355165487578197E-5	0.0	0.012433997467035753	6.532748231367296E-4
32-33	0.0	6.532748231367295E-5	0.0	0.029767556107596974	6.532748231367296E-4
34-35	0.0	8.710330975156394E-5	0.0	0.05234908916068993	6.532748231367296E-4
36-37	0.0	8.710330975156394E-5	0.0	0.08499105449008851	6.532748231367296E-4
38-39	0.0	8.710330975156394E-5	0.0	0.14121624093472304	7.186023054504024E-4
40-41	0.0	8.710330975156394E-5	0.0	0.20902616757631556	7.839297877640755E-4
42-43	0.0	8.710330975156394E-5	0.0	0.2703904492962924	8.057056152019665E-4
44-45	0.0	8.710330975156394E-5	0.0	0.3364800855702915	8.274814426398574E-4
46-47	0.0	8.710330975156394E-5	0.0	0.4036367373887473	8.274814426398574E-4
48-49	0.0	8.710330975156394E-5	0.0	0.4802223224878099	9.145847523914214E-4
50-51	0.0	8.710330975156394E-5	0.0	0.5642116889157553	9.145847523914214E-4
52-53	0.0	8.710330975156394E-5	0.0	0.651576308596574	0.0010016880621429853
54-55	0.0	8.710330975156394E-5	0.0	0.7394635481359021	0.0011105671993324401
56-57	0.0	1.7420661950312788E-4	0.0	0.8341230700084141	0.0011323430267703312
58-59	0.0	1.7420661950312788E-4	0.0	0.9365347864488155	0.0011758946816461133
60-61	0.0	1.7420661950312788E-4	0.0	1.047504403072308	0.0011758946816461133
62-63	0.0	1.7420661950312788E-4	0.0	1.1686651069367335	0.001219446336521895
64-65	0.0	1.7420661950312788E-4	0.0	1.2869078499244815	0.001241222163959786
66-67	0.0	1.7420661950312788E-4	0.0	1.4189782433352902	0.0012629979913976771
68-69	0.0	1.7420661950312788E-4	0.0	1.5615881372260383	0.0012629979913976771
70-71	0.0	1.7420661950312788E-4	0.0	1.719462886150748	0.0013065496462734592
72-73	0.0	1.7420661950312788E-4	0.0	1.8774900658675229	0.001371877128587132
74-75	0.0	1.7420661950312788E-4	0.0	2.038914274664609	0.001393652956025023
76-77	0.0	1.7420661950312788E-4	0.0	2.2188914884387776	0.001393652956025023
78-79	0.0	2.1775827437890986E-4	0.0	2.420339668066707	0.001437204610900805
80-81	0.0	2.1775827437890986E-4	0.0	2.6279068552046843	0.0014807562657765871
82-83	0.0	2.1775827437890986E-4	0.0	2.8493887960754734	0.001524307920652369
84-85	0.0	2.1775827437890986E-4	0.0	3.0862880227722895	0.001524307920652369
86-87	0.0	2.613099292546918E-4	0.0	3.3298506526651	0.00154608374809026
88-89	0.0	3.048615841304738E-4	0.0	3.6044220608294673	0.001567859575528151
90-91	0.0	3.048615841304738E-4	0.0	3.8960221660502654	0.001567859575528151
92-93	0.0	3.048615841304738E-4	0.0	4.198379530025382	0.0016114112304039328
94-95	0.0	3.048615841304738E-4	0.0	4.5079229170550015	0.0016985145401554969
96-97	0.0	3.048615841304738E-4	0.0	4.83556201668551	0.0017202903675933878
98-99	0.0	3.048615841304738E-4	0.0	5.203029104699921	0.0017856178499070607
100-101	0.0	3.048615841304738E-4	0.0	5.601722729260267	0.0017856178499070607
102-103	0.0	3.048615841304738E-4	0.0	6.021364699815864	0.0018509453322207337
104-105	0.0	3.048615841304738E-4	0.0	6.455444043962782	0.0018727211596586248
106-107	0.0	3.048615841304738E-4	0.0	6.918115049535652	0.0018727211596586248
108-109	0.0	3.048615841304738E-4	0.0	7.409617250636289	0.0018727211596586248
110-111	0.0	3.048615841304738E-4	0.0	7.918322355412862	0.0018944969870965157
112-113	0.0	3.048615841304738E-4	2.1775827437890985E-5	8.4440779330733	0.0019162728145344066
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2270	0.0	66.09388	1
GTATCAA	4160	0.0	57.61883	1
GTACATG	11130	0.0	41.353184	1
TCAACGC	5865	0.0	39.628723	4
TACATGG	11905	0.0	38.49554	2
ACATGGG	12170	0.0	37.804214	3
CAACGCA	6145	0.0	37.532078	5
CATGGGG	11015	0.0	36.898895	4
TATCAAC	6475	0.0	36.53964	2
ATCAACG	6395	0.0	36.344402	3
AACGCAG	6455	0.0	35.62407	6
ATGGGGG	6955	0.0	34.3606	5
TGGGGGG	4900	0.0	28.57092	6
ACGCAGA	8030	0.0	28.330761	7
CGCAGAG	8560	0.0	26.434036	8
AGAGTAC	8580	0.0	23.595346	10-11
GCAGAGT	9645	0.0	23.460377	9
GTATACG	155	2.0425525E-4	23.138378	1
TAAGTAC	1695	0.0	22.92224	1
ATGGGGA	3930	0.0	21.988161	5
>>END_MODULE