FastQCFastQC Report
Mon 15 Aug 2016
SRR2921346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386009
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.061.9259261
GTATCAA24600.054.6721341
TCAACGC29500.043.1671264
GTACATG79400.041.3724561
CAACGCA31200.041.1965185
TACATGG80000.040.0922242
AACGCAG32750.039.2439276
ATCAACG32800.039.0055123
ACATGGG84000.038.324753
TATCAAC34350.038.2848432
CATGGGG74700.035.4487344
ATGGGGG47950.034.375785
ACGCAGA41100.031.4101187
CGCAGAG43200.029.8832388
TGGGGGG34650.029.3645176
GCAGAGT49850.025.5378659
GTATATG5450.024.0225371
TATACCG1501.6856712E-423.8024335
GAGTACA53650.023.2939011
AGAGTAC43250.023.03905710-11
Produced by FastQC (version 0.11.5)