##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921346_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386009
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.222554110398995	31.0	31.0	34.0	26.0	34.0
2	30.389697325197744	31.0	31.0	34.0	27.0	34.0
3	30.655000075757084	33.0	31.0	34.0	27.0	34.0
4	34.05828244982536	37.0	35.0	37.0	30.0	37.0
5	34.23652948862525	37.0	35.0	37.0	31.0	37.0
6	34.23458938578321	37.0	35.0	37.0	31.0	37.0
7	34.113380216145785	37.0	35.0	37.0	30.0	37.0
8	34.190062979389026	37.0	35.0	37.0	31.0	37.0
9	35.78888088028288	39.0	37.0	39.0	30.0	39.0
10-11	35.81726236986917	39.0	37.0	39.0	30.5	39.0
12-13	35.75019570579989	39.0	37.0	39.0	30.0	39.0
14-15	37.07774877363711	41.0	38.0	41.0	31.0	41.0
16-17	37.066514719601386	41.0	38.0	41.0	31.0	41.0
18-19	37.051622680660806	41.0	38.0	41.0	30.5	41.0
20-21	37.08995829031413	41.0	38.0	41.0	31.0	41.0
22-23	37.047489229867914	41.0	38.0	41.0	30.0	41.0
24-25	37.032422588886504	41.0	38.0	41.0	30.0	41.0
26-27	37.00165511190764	41.0	38.0	41.0	30.0	41.0
28-29	36.94417352268275	40.0	38.0	41.0	30.0	41.0
30-31	36.86006440073621	40.0	38.0	41.0	30.0	41.0
32-33	36.78082682002787	40.0	38.0	41.0	30.0	41.0
34-35	36.675346263985304	40.0	38.0	41.0	30.0	41.0
36-37	36.560964972088925	40.0	37.0	41.0	30.0	41.0
38-39	36.46437180422349	40.0	37.0	41.0	29.0	41.0
40-41	36.35852148146224	40.0	37.0	41.0	29.0	41.0
42-43	36.16945272361146	40.0	36.5	41.0	28.0	41.0
44-45	35.91574369286202	40.0	36.0	41.0	27.5	41.0
46-47	35.703990738876875	40.0	35.5	41.0	27.0	41.0
48-49	35.50123989093866	40.0	35.0	41.0	26.5	41.0
50-51	35.31761698517109	39.0	35.0	41.0	26.0	41.0
52-53	35.1283501766583	39.0	35.0	41.0	26.0	41.0
54-55	34.922904541023904	39.0	35.0	41.0	26.0	41.0
56-57	34.67949594843901	38.5	35.0	40.0	25.0	41.0
58-59	34.408052184365324	38.0	34.0	40.0	24.0	41.0
60-61	34.121498489548046	37.5	34.0	40.0	23.5	41.0
62-63	33.78664785005003	37.0	34.0	40.0	23.0	41.0
64-65	33.444460678105266	36.5	34.0	39.5	22.0	41.0
66-67	33.1001761171825	36.0	33.0	39.0	22.0	41.0
68-69	32.752617767994295	35.5	33.0	39.0	21.0	40.0
70-71	32.70026709783269	35.0	33.0	38.5	22.0	40.0
72-73	32.412240468856986	35.0	33.0	37.0	20.5	39.5
74-75	32.084277591271054	35.0	33.0	37.0	20.0	39.0
76-77	31.772874490713985	35.0	33.0	36.5	19.5	39.0
78-79	31.483651621309818	35.0	33.0	36.0	18.5	37.5
80-81	31.203591390820694	35.0	33.0	35.5	17.5	37.0
82-83	30.986341719281768	35.0	32.5	35.0	16.5	37.0
84-85	30.772709628869656	35.0	32.0	35.0	13.0	36.0
86-87	30.60068729712433	35.0	32.0	35.0	10.5	36.0
88-89	30.459659713609362	35.0	32.0	35.0	7.5	36.0
90-91	30.336468233611757	35.0	32.0	35.0	7.0	35.0
92-93	30.225115060580414	35.0	32.0	35.0	6.5	35.0
94-95	30.10213101069329	35.0	32.0	35.0	2.0	35.0
96-97	30.00699562556953	35.0	31.5	35.0	2.0	35.0
98-99	29.9208663147209	35.0	31.0	35.0	2.0	35.0
100-101	29.827926081288073	35.0	31.0	35.0	2.0	35.0
102-103	28.981956466372154	33.5	29.5	34.5	2.0	35.0
104-105	29.577551083723122	34.0	31.0	35.0	2.0	35.0
106-107	29.650881415632945	34.5	31.0	35.0	2.0	35.0
108-109	29.585783714247164	35.0	31.0	35.0	2.0	35.0
110-111	29.505910134782674	34.0	31.0	35.0	2.0	35.0
112-113	29.4153811411037	34.0	31.0	35.0	2.0	35.0
114-115	29.28730946191547	34.0	31.0	35.0	2.0	35.0
116-117	29.1452707017054	34.0	30.5	35.0	2.0	35.0
118-119	29.148118085813294	34.5	30.5	35.0	2.0	35.0
120-121	29.325492835905106	35.0	31.0	35.0	2.0	35.0
122-123	29.190014999902598	35.0	31.0	35.0	2.0	35.0
124-125	28.601425748317652	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47065.0
3	6460.0
4	4264.0
5	3623.0
6	3968.0
7	4430.0
8	4679.0
9	4805.0
10	5426.0
11	5843.0
12	5659.0
13	5685.0
14	5818.0
15	5170.0
16	4366.0
17	4224.0
18	3861.0
19	3625.0
20	3648.0
21	3931.0
22	4407.0
23	5584.0
24	7853.0
25	9899.0
26	10868.0
27	11990.0
28	14434.0
29	17333.0
30	21078.0
31	26496.0
32	34523.0
33	48179.0
34	77579.0
35	128469.0
36	265433.0
37	445691.0
38	119588.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.741354177791756	17.874358833734078	12.530849586795945	24.853437401678217
2	16.49290009185612	19.185590494875758	39.8008316839399	24.520677729328224
3	20.10529171693615	23.886254351076296	28.1537726645616	27.854681267425953
4	13.775302562800002	14.900550429057574	34.80294812920507	36.521198878937355
5	14.550471279818918	37.9325596883485	32.55124622359221	14.96572280824038
6	34.04679440499483	34.1104494609889	17.1710596113	14.671696522716266
7	30.580584733880922	31.900807243616992	19.305079205413087	18.213528817089003
8	27.775905650561	35.22207317363567	18.66062647069223	18.3413947051111
9	28.445206734772515	16.227066950865947	18.037899330534636	37.289826983826906
10-11	27.034207472999416	25.50213002995896	26.119095516546697	21.34456698049493
12-13	26.422310475253152	22.813175997933495	26.550889882056172	24.213623644757178
14-15	24.235265680440286	24.167873744744025	23.83935609675144	27.75750447806425
16-17	24.63776533781451	27.20280126455189	24.41246787842668	23.746965519206924
18-19	24.525000496059484	26.663720373974737	25.6948350286542	23.116444101311583
20-21	25.485142499673508	25.59766597663812	25.388564959489386	23.52862656419899
22-23	25.330590051019858	24.971878109371968	25.35486975902841	24.342662080579768
24-25	24.78895962751406	25.0098940676361	25.437368297062303	24.763778007787543
26-27	24.284079503991148	25.487014978249466	25.544845639394982	24.684059878364405
28-29	24.45513924790438	26.19581471613775	25.193597789206144	24.155448246751725
30-31	25.457706438699734	25.44017311094628	25.228763027334672	23.87335742301931
32-33	24.968098313116005	25.46344483380276	25.216853903803198	24.351602949278032
34-35	24.8507019793961	25.826059192475658	25.47852392213738	23.84471490599086
36-37	24.812208562950403	25.74531220405329	25.407231697527806	24.03524753546851
38-39	24.86685710616148	25.921646101462066	25.20291619195089	24.008580600425567
40-41	24.92030507031481	25.520813039807777	25.343745264844035	24.21513662503337
42-43	24.67487113178903	25.576263139362382	25.14376814689533	24.605097581953256
44-45	24.233680397717023	25.612305272348134	25.709569885490403	24.444444444444443
46-47	24.23006473506535	25.42493773809339	25.506185783107405	24.838811743733853
48-49	24.274370445198066	25.738364961396925	25.481708636986795	24.505555956418213
50-51	24.42156127767007	25.71563911282613	25.539872533152852	24.322927076350954
52-53	24.488369785794813	25.69138218714769	25.345406989853437	24.474841037204058
54-55	24.059074031583307	25.267403216054007	25.937967648973032	24.735555103389657
56-57	24.339871780533297	25.610557722210398	25.303285578737363	24.746284918518946
58-59	24.340363110553756	25.74737682136121	25.59368763141147	24.31857243667356
60-61	24.946363021420517	25.611869222096956	25.544874859075534	23.89689289740699
62-63	24.677292969640646	25.696318434093445	25.449264466077704	24.177124130188204
64-65	24.41261642238874	25.307666804597982	25.533179095577026	24.746537677436255
66-67	24.425431454024775	25.492422903229762	25.41276652238568	24.66937912035978
68-69	24.168983413941465	25.50473583308741	25.64503944302822	24.681241309942905
70-71	24.251619385364112	25.31602140781454	25.769359118433844	24.663000088387506
72-73	24.303040229503562	25.91266050385729	25.22251917099343	24.561780095645723
74-75	24.1742673442279	25.81534267806005	25.41929693041096	24.591093047301097
76-77	24.137970842998822	25.2937526380917	25.467821594652023	25.100454924257456
78-79	24.030194222706925	25.36922949824328	25.80859988434014	24.79197639470966
80-81	24.351317927449774	25.677109939363508	25.31158817977956	24.659983953407156
82-83	24.484589825641173	25.65679487133235	25.534025765999125	24.32458953702735
84-85	24.478371629795294	25.783863136433876	25.167718768591573	24.570046465179257
86-87	23.939615213664357	26.056704899012345	25.37985817281328	24.623821714510015
88-89	23.85028682626673	25.553278326280974	25.58549464678755	25.010940200664745
90-91	24.39349608747885	25.28181336065544	25.694927972812575	24.62976257905313
92-93	24.537366913955097	25.431696477050806	25.51640686529353	24.514529743700567
94-95	24.385478185891632	25.455385070512982	25.338603016779526	24.820533726815857
96-97	23.966178841138973	25.377522408177345	25.58396193604343	25.072336814640252
98-99	23.734323578795006	25.40768977119153	25.45383243898092	25.404154211032537
100-101	23.83278368792407	25.61531603979671	25.592695254000397	24.959205018278823
102-103	23.80053725540845	25.579158588474332	25.589187933327224	25.03111622278999
104-105	24.001662407828903	25.60775472846437	25.43520054778647	24.95538231592026
106-107	24.04391062597319	25.43469759132293	25.58445522279289	24.93693655991099
108-109	23.94718737068594	25.45481570525251	25.548977086314963	25.049019837746595
110-111	23.705716565817784	25.9539635558647	25.645644497229963	24.694675381087553
112-113	23.719423235382823	26.028292751912424	25.528558997588636	24.723725015116116
114-115	23.819475559769202	26.18654908861861	25.380905688638954	24.613069662973235
116-117	23.69192983797914	26.044913881209713	25.38194548413432	24.881210796676825
118-119	23.63497420184706	25.675882701507426	25.654203581390643	25.034939515254873
120-121	23.60500528012821	25.716082437106586	25.844595021201688	24.834317261563516
122-123	23.542059439573038	25.979289729498067	25.55006244683361	24.928588384095285
124-125	23.44782603833307	25.9105898446843	25.45632758254337	25.185256534439265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	322.0
1	275.0
2	203.5
3	170.5
4	144.5
5	130.5
6	129.0
7	166.5
8	219.5
9	239.5
10	241.5
11	231.5
12	258.0
13	283.0
14	304.5
15	345.5
16	367.5
17	439.5
18	466.5
19	462.5
20	543.5
21	613.0
22	680.5
23	821.0
24	977.0
25	1231.0
26	1726.0
27	2299.5
28	2965.5
29	3919.5
30	5344.5
31	7091.0
32	9360.0
33	11956.5
34	14756.5
35	17594.5
36	20648.5
37	23600.5
38	25850.5
39	28807.0
40	31819.0
41	34224.5
42	36676.5
43	39996.5
44	43837.0
45	47016.5
46	50518.0
47	54310.0
48	58801.0
49	62924.5
50	64945.0
51	65902.5
52	65235.5
53	63493.5
54	62065.5
55	59452.5
56	55760.5
57	51174.0
58	45669.0
59	39850.5
60	34058.5
61	29111.5
62	24230.5
63	18829.0
64	13921.0
65	10502.0
66	8101.0
67	6325.5
68	4934.0
69	3840.0
70	2991.0
71	2270.0
72	1763.5
73	1447.5
74	1141.5
75	833.0
76	622.0
77	432.0
78	291.0
79	190.0
80	125.5
81	85.0
82	63.5
83	47.0
84	30.0
85	21.5
86	17.0
87	14.5
88	15.0
89	16.0
90	9.5
91	5.5
92	4.5
93	3.0
94	4.0
95	3.5
96	1.5
97	1.0
98	1.0
99	1.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01825384972247655
2	0.010533842132338246
3	0.010461692528692093
4	0.01305907825995358
5	0.007359259571907541
6	0.030014235116799386
7	0.013131227863599732
8	0.013131227863599732
9	0.00916299966306135
10-11	0.008152905212015218
12-13	0.006854212346384475
14-15	0.00624094071539218
16-17	0.004581499831530675
18-19	0.00624094071539218
20-21	0.00548336987710758
22-23	0.005627669084399885
24-25	0.005591594282576809
26-27	0.004761873840646057
28-29	0.005880192697161418
30-31	0.005555519480753732
32-33	0.007972531202899837
34-35	0.007683932788315227
36-37	0.006962436751853704
38-39	0.006637763535446019
40-41	0.007142810760969085
42-43	0.00699851155367678
44-45	0.007142810760969085
46-47	0.009343373672176731
48-49	0.007864306797430609
50-51	0.005771968291692189
52-53	0.005158696660699895
54-55	0.006277015517215256
56-57	0.007503558779199847
58-59	0.006854212346384475
60-61	0.005158696660699895
62-63	0.005555519480753732
64-65	0.0038600037950691514
66-67	0.004040377804184533
68-69	0.006204865913569104
70-71	0.004797948642469132
72-73	0.004401125822415294
74-75	0.004184677011476837
76-77	0.0046175746333537515
78-79	0.00349925577683839
80-81	0.0032467321640768563
82-83	0.005339070669815275
84-85	0.00955982248311519
86-87	0.00699851155367678
88-89	0.00472579903882298
90-91	0.005339070669815275
92-93	0.007972531202899837
94-95	0.006782062742738322
96-97	0.00945159807764596
98-99	0.0064934643281537125
100-101	0.008333279221130599
102-103	0.00548336987710758
104-105	0.004870098246115285
106-107	0.007431409175553695
108-109	0.00652953912997679
110-111	0.007720007590138303
112-113	0.005122621858876818
114-115	0.0025973857312614856
116-117	0.003282806965899933
118-119	0.15793548238142752
120-121	0.011002814556038236
122-123	0.0021644881093845713
124-125	0.0018398148929768853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1386009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.78900456512284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.6596292253838	53.55435095234752
2	9.531720920000526	12.351014203987818
3	2.966376016857299	5.765656478941153
4	1.4501960757321015	3.758270406837214
5	0.8570450889834476	2.776354909133235
6	0.5423256576113558	2.1082043704059226
7	0.38337105804554183	1.7386760460894009
8	0.280197055020229	1.4522950621471675
9	0.20364775139751157	1.1874721585473342
>10	1.0744529144491588	12.138056188049825
>50	0.03757717461175289	1.61988167894944
>100	0.013238574931504848	1.3845749715262987
>500	0.0	0.0
>1k	2.2248697577475086E-4	0.16519257303766713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4429920729230474E-4
2	0.0	0.0	0.0	0.0	1.4429920729230474E-4
3	0.0	0.0	0.0	0.0	1.4429920729230474E-4
4	0.0	0.0	0.0	0.0	1.4429920729230474E-4
5	0.0	0.0	0.0	0.0	1.4429920729230474E-4
6	0.0	0.0	0.0	0.0	2.1644881093845712E-4
7	0.0	0.0	0.0	0.0	2.1644881093845712E-4
8	0.0	0.0	0.0	0.0	2.1644881093845712E-4
9	0.0	0.0	0.0	0.0	2.1644881093845712E-4
10-11	0.0	0.0	0.0	0.0	2.1644881093845712E-4
12-13	0.0	0.0	0.0	0.0	3.2467321640768565E-4
14-15	0.0	0.0	0.0	0.0	3.96822820053838E-4
16-17	0.0	0.0	0.0	0.0	5.050472255230666E-4
18-19	0.0	0.0	0.0	0.0	5.771968291692189E-4
20-21	0.0	0.0	0.0	3.6074801823076184E-5	5.771968291692189E-4
22-23	0.0	0.0	0.0	1.4429920729230474E-4	5.771968291692189E-4
24-25	0.0	0.0	0.0	5.050472255230666E-4	5.771968291692189E-4
26-27	0.0	0.0	0.0	9.379448473999809E-4	5.771968291692189E-4
28-29	0.0	0.0	0.0	0.0036435549841306947	5.771968291692189E-4
30-31	0.0	0.0	0.0	0.013924873503707406	6.132716309922952E-4
32-33	0.0	0.0	0.0	0.03820321513063768	6.493464328153713E-4
34-35	0.0	1.4429920729230474E-4	0.0	0.06713520619274478	7.214960364615237E-4
36-37	0.0	2.1644881093845712E-4	0.0	0.10692571260359782	7.214960364615237E-4
38-39	0.0	2.1644881093845712E-4	0.0	0.1735919463726426	7.575708382845999E-4
40-41	0.0	2.1644881093845712E-4	0.0	0.26110941559542544	7.936456401076761E-4
42-43	0.0	2.1644881093845712E-4	0.0	0.3340165900798624	7.936456401076761E-4
44-45	0.0	2.1644881093845712E-4	0.0	0.410062272322907	8.657952437538285E-4
46-47	0.0	2.1644881093845712E-4	0.0	0.49317861572327454	0.0010100944510461332
48-49	0.0	2.1644881093845712E-4	0.0	0.5862516044268111	0.0010100944510461332
50-51	0.0	2.1644881093845712E-4	0.0	0.6875857227478321	0.0010100944510461332
52-53	0.0	2.8859841458460947E-4	0.0	0.7954854550006529	0.0010461692528692095
54-55	0.0	2.8859841458460947E-4	0.0	0.910527998014443	0.0010822440546922856
56-57	0.0	2.8859841458460947E-4	0.0	1.0215662380258714	0.0010822440546922856
58-59	0.0	2.8859841458460947E-4	0.0	1.1510026269670688	0.0010822440546922856
60-61	0.0	2.8859841458460947E-4	0.0	1.2914057556624812	0.0010822440546922856
62-63	0.0	2.8859841458460947E-4	0.0	1.459297883347078	0.0010822440546922856
64-65	0.0	2.8859841458460947E-4	0.0	1.608034291263621	0.0010822440546922856
66-67	0.0	2.8859841458460947E-4	0.0	1.7630837894992024	0.0010822440546922856
68-69	0.0	2.8859841458460947E-4	0.0	1.9384073263593526	0.0011183188565153618
70-71	0.0	3.2467321640768565E-4	0.0	2.126212744650287	0.0011543936583384379
72-73	0.0	3.607480182307618E-4	0.0	2.298000950931776	0.001370842469276895
74-75	0.0	3.607480182307618E-4	0.0	2.4805394481565415	0.001370842469276895
76-77	0.0	3.607480182307618E-4	0.0	2.6893764759103296	0.001370842469276895
78-79	0.0	3.607480182307618E-4	0.0	2.919281187928794	0.001370842469276895
80-81	0.0	3.607480182307618E-4	0.0	3.1443879513047897	0.0014790668747461236
82-83	0.0	3.607480182307618E-4	0.0	3.3930876350730768	0.001731590487507657
84-85	0.0	3.607480182307618E-4	0.0	3.64467330298721	0.001731590487507657
86-87	0.0	3.607480182307618E-4	0.0	3.924325166719697	0.001731590487507657
88-89	0.0	3.607480182307618E-4	0.0	4.231429954639545	0.0018037400911538092
90-91	0.0	3.607480182307618E-4	0.0	4.555670273425353	0.0018037400911538092
92-93	0.0	3.607480182307618E-4	0.0	4.869665348493408	0.0018758896947999616
94-95	0.0	3.607480182307618E-4	0.0	5.1908393091242555	0.0021644881093845713
96-97	0.0	3.607480182307618E-4	0.0	5.551875925769602	0.0021644881093845713
98-99	0.0	3.607480182307618E-4	0.0	5.9539295920877855	0.002200562911207647
100-101	0.0	3.607480182307618E-4	0.0	6.378890757563624	0.0022366377130307235
102-103	3.6074801823076184E-5	3.607480182307618E-4	0.0	6.826326524575237	0.0022366377130307235
104-105	7.214960364615237E-5	4.3289762187691423E-4	0.0	7.297391286780966	0.0022366377130307235
106-107	7.214960364615237E-5	4.3289762187691423E-4	0.0	7.797496264454272	0.0022366377130307235
108-109	7.214960364615237E-5	4.3289762187691423E-4	0.0	8.311742564442223	0.0022366377130307235
110-111	7.214960364615237E-5	4.3289762187691423E-4	0.0	8.867979933752235	0.0022366377130307235
112-113	7.214960364615237E-5	4.3289762187691423E-4	0.0	9.419094681203369	0.0022366377130307235
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1355	0.0	61.925926	1
GTATCAA	2460	0.0	54.672134	1
TCAACGC	2950	0.0	43.167126	4
GTACATG	7940	0.0	41.372456	1
CAACGCA	3120	0.0	41.196518	5
TACATGG	8000	0.0	40.092224	2
AACGCAG	3275	0.0	39.243927	6
ATCAACG	3280	0.0	39.005512	3
ACATGGG	8400	0.0	38.32475	3
TATCAAC	3435	0.0	38.284843	2
CATGGGG	7470	0.0	35.448734	4
ATGGGGG	4795	0.0	34.37578	5
ACGCAGA	4110	0.0	31.410118	7
CGCAGAG	4320	0.0	29.883238	8
TGGGGGG	3465	0.0	29.364517	6
GCAGAGT	4985	0.0	25.537865	9
GTATATG	545	0.0	24.022537	1
TATACCG	150	1.6856712E-4	23.802433	5
GAGTACA	5365	0.0	23.293901	1
AGAGTAC	4325	0.0	23.039057	10-11
>>END_MODULE