FastQCFastQC Report
Mon 15 Aug 2016
SRR2921346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386009
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20490.1478345378709662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.11623301147395146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.11341917693175152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.068.248371
GTATCAA33850.048.5450741
GTACATG74950.038.0292631
TACATGG76900.036.3458372
ACATGGG79050.035.432693
TCAACGC45750.034.7799034
CATGGGG69300.034.398144
ATGGGGG46250.033.3730745
TATCAAC48800.033.216762
CAACGCA47600.033.1777655
ATCAACG48400.032.7525023
AACGCAG49050.032.436456
TAGCCCG1203.656622E-529.7973865
TGGGGGG35850.029.4201726
GTGGTAT9500.028.3047981
TGGTATC9400.027.261442
ACGCAGA60000.026.2160177
CGCAGAG63850.024.7223158
GTAAGTA8000.023.0929762
AGAGTAC64050.022.691710-11
Produced by FastQC (version 0.11.5)