##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386009
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.163996049087704	33.0	30.0	34.0	16.0	34.0
2	29.95772754722372	33.0	31.0	34.0	26.0	34.0
3	30.19853550734519	33.0	31.0	34.0	26.0	34.0
4	32.31700154905199	37.0	35.0	37.0	19.0	37.0
5	31.987518840065253	37.0	33.0	37.0	19.0	37.0
6	32.124664414156044	37.0	35.0	37.0	17.0	37.0
7	32.35891758278626	37.0	35.0	37.0	17.0	37.0
8	32.60908767547686	37.0	35.0	37.0	17.0	37.0
9	34.359257407419435	39.0	37.0	39.0	17.0	39.0
10-11	34.562324270621616	39.0	37.0	39.0	21.5	39.0
12-13	34.58883203500122	39.0	37.0	39.0	24.0	39.0
14-15	35.984395483723404	41.0	38.0	41.0	25.0	41.0
16-17	35.99368474519285	41.0	38.0	41.0	25.0	41.0
18-19	36.02544680445798	41.0	38.0	41.0	25.0	41.0
20-21	36.01958825664191	41.0	38.0	41.0	25.0	41.0
22-23	35.975235009296476	41.0	38.0	41.0	25.0	41.0
24-25	35.90967879717953	41.0	38.0	41.0	24.5	41.0
26-27	35.8089738234023	40.5	38.0	41.0	24.0	41.0
28-29	35.73923545951	40.0	37.0	41.0	23.5	41.0
30-31	35.631846546451	40.0	37.0	41.0	22.5	41.0
32-33	35.49329621957722	40.0	37.0	41.0	21.0	41.0
34-35	35.40606482353289	40.0	36.0	41.0	19.5	41.0
36-37	35.32802745148119	40.0	36.0	41.0	18.5	41.0
38-39	35.23264639695702	40.0	36.0	41.0	17.0	41.0
40-41	35.09642542003695	40.0	35.5	41.0	15.5	41.0
42-43	34.94807898072812	40.0	35.0	41.0	13.0	41.0
44-45	34.79221274897927	40.0	35.0	41.0	8.5	41.0
46-47	34.57368242197562	40.0	35.0	41.0	4.5	41.0
48-49	34.35054858951132	40.0	35.0	41.0	2.0	41.0
50-51	34.16157110090916	40.0	35.0	41.0	2.0	41.0
52-53	33.98074254929081	39.0	35.0	41.0	2.0	41.0
54-55	33.774934722646094	39.0	35.0	41.0	2.0	41.0
56-57	33.532452170224005	39.0	34.5	41.0	2.0	41.0
58-59	33.47153048789727	38.5	35.0	41.0	2.0	41.0
60-61	33.23324487791926	38.0	34.0	41.0	2.0	41.0
62-63	32.93932687305782	37.0	34.0	40.0	2.0	41.0
64-65	32.64445468968816	37.0	34.0	40.0	2.0	41.0
66-67	32.32912123947247	36.0	34.0	39.0	2.0	41.0
68-69	32.00094335606767	35.5	34.0	39.0	2.0	41.0
70-71	31.669965346545368	35.0	33.0	38.5	2.0	40.5
72-73	31.33101805255233	35.0	33.0	37.0	2.0	39.5
74-75	30.996748217363667	35.0	33.0	37.0	2.0	39.0
76-77	30.701693856244802	35.0	33.0	36.0	2.0	39.0
78-79	30.446216077961978	35.0	33.0	36.0	2.0	37.5
80-81	30.225991317516698	35.0	33.0	35.5	2.0	37.0
82-83	30.024175528441734	35.0	32.0	35.0	2.0	37.0
84-85	29.852211637875367	35.0	32.0	35.0	2.0	36.0
86-87	29.70998023822356	35.0	32.0	35.0	2.0	36.0
88-89	29.592555315297375	35.0	32.0	35.0	2.0	36.0
90-91	29.491342769058498	35.0	32.0	35.0	2.0	35.0
92-93	29.419863795978237	35.0	32.0	35.0	2.0	35.0
94-95	29.351110634923728	35.0	32.0	35.0	2.0	35.0
96-97	29.28633544226625	35.0	32.0	35.0	2.0	35.0
98-99	29.23129864236091	35.0	31.5	35.0	2.0	35.0
100-101	29.18164636737568	35.0	31.0	35.0	2.0	35.0
102-103	28.510675255355487	34.0	29.5	35.0	2.0	35.0
104-105	29.043038681567005	35.0	31.0	35.0	2.0	35.0
106-107	29.100631741929526	35.0	31.0	35.0	2.0	35.0
108-109	29.17016051122323	35.0	31.5	35.0	2.0	35.0
110-111	29.28020164371227	35.0	32.0	35.0	2.0	35.0
112-113	29.24132022230736	35.0	32.0	35.0	2.0	35.0
114-115	29.177458082884023	35.0	32.0	35.0	2.0	35.0
116-117	29.110716813527183	35.0	32.0	35.0	2.0	35.0
118-119	29.03111704180853	35.0	32.0	35.0	2.0	35.0
120-121	28.932189473517127	35.0	32.0	35.0	2.0	35.0
122-123	28.81743336442981	35.0	32.0	35.0	2.0	35.0
124-125	28.293138789142063	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94783.0
3	5991.0
4	4539.0
5	4198.0
6	4280.0
7	4442.0
8	4875.0
9	4803.0
10	4929.0
11	5001.0
12	5618.0
13	6719.0
14	8242.0
15	7273.0
16	4493.0
17	3991.0
18	3608.0
19	3300.0
20	3111.0
21	3142.0
22	3483.0
23	4424.0
24	5919.0
25	7523.0
26	8883.0
27	10256.0
28	12048.0
29	14737.0
30	17279.0
31	21573.0
32	27913.0
33	38006.0
34	58817.0
35	100184.0
36	221155.0
37	476334.0
38	169861.0
39	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26983924710707	17.881713905899968	12.199828256075115	26.648618590917845
2	15.90292703727032	19.378301295301835	38.42810544520274	26.290666222225106
3	19.32303469890888	23.346529495840215	28.460782000694078	28.869653804556826
4	13.517396550131622	15.99172170138087	31.656265875398326	38.83461587308918
5	14.255285316757615	37.857395829568866	32.038740920096856	15.848577933576669
6	34.06369397326149	33.73742098527224	16.551929738700093	15.646955302766175
7	30.396769209341063	31.746083294182515	18.56275714150658	19.294390354969845
8	28.249602239724076	34.6240128149161	17.95228319810085	19.17410174725897
9	28.176360658340005	16.050537192169767	17.880237533461766	37.89286461602846
10-11	27.31371901780512	25.44954734113649	25.172703020070674	22.064030620987715
12-13	26.626253744494026	22.770205468450236	26.171121075974202	24.432419711081536
14-15	24.47274873395483	23.84876288682108	23.694939931847482	27.983548447376606
16-17	24.401537075156078	26.773924267447036	24.259727029189566	24.56481162820732
18-19	24.388009024472424	25.910113137793477	26.067868246165787	23.634009591568308
20-21	25.229814525013904	25.090241116760424	25.92151277516957	23.758431583056097
22-23	24.947962098370212	24.432236731507516	25.88973087476344	24.730070295358832
24-25	24.597206800244443	24.613332236659357	25.860185612070342	24.929275351025858
26-27	24.08624330722239	24.93811367747251	26.060761510206643	24.914881505098453
28-29	24.22011689678783	25.582409638032654	25.814947810584204	24.382525654595316
30-31	25.109577210537594	25.058928188777998	25.783310209385363	24.048184391299046
32-33	24.627365334568534	25.101965427352923	25.819890058433963	24.450779179644577
34-35	24.666362195339282	25.419676207008756	26.022738669085122	23.89122292856684
36-37	24.69511381239227	25.180428121318116	26.157874876714367	23.966583189575246
38-39	24.55294301840753	25.48536842112858	25.894456673802264	24.06723188666163
40-41	24.61488345313775	25.286668412687074	25.938251483215474	24.160196650959698
42-43	24.38418509547918	25.387605708188044	25.589841047208207	24.638368149124574
44-45	23.958574583570524	25.45690540249017	26.115775582986835	24.46874443095247
46-47	23.990969755607647	25.29399159745716	25.62587977422946	25.08915887270573
48-49	24.194756311106204	25.66516523341479	25.407699372803492	24.73237908267551
50-51	24.214922125325305	25.739335025963033	25.568989811754467	24.47675303695719
52-53	24.311927267427556	25.686810114508635	25.41805284092672	24.58320977713709
54-55	23.920227069232595	25.230103123428492	25.962962722464283	24.88670708487463
56-57	24.080507413732523	25.465996252549587	25.411703675805857	25.041792657912033
58-59	24.106445196243314	25.606687979659583	25.621514723208865	24.66535210088823
60-61	24.717660563531695	25.391393562379466	25.76704047376316	24.123905400325683
62-63	24.38505089072293	25.48031794887335	25.649400545018107	24.485230615385614
64-65	24.092844995956014	25.1108398286014	25.686665815301346	25.10964936014124
66-67	24.09428798802894	25.372057468602293	25.594025724219684	24.939628819149082
68-69	23.85356083546355	25.296047861161075	25.730893522336434	25.11949778103894
70-71	23.920227069232595	25.203083096863004	25.84950747073071	25.02718236317369
72-73	23.976503760076596	25.714912385128812	25.349438567859227	24.959145286935367
74-75	23.90139602268095	25.639047076894883	25.455823158435482	25.003733741988686
76-77	23.777587302824152	25.100305986469063	25.535620620068123	25.58648609063866
78-79	23.733395670590884	25.130464520793154	25.915343984057827	25.220795824558138
80-81	23.96896412649557	25.408276569632665	25.384503275231257	25.238256028640503
82-83	24.070190020411122	25.453622595524273	25.547777828282502	24.928409555782103
84-85	23.995154432619124	25.585548145791257	25.196589632534856	25.22270778905476
86-87	23.478491120909027	25.87108020222091	25.364084937399394	25.286343739470666
88-89	23.397178517599816	25.456941477291995	25.573066264360477	25.572813740747712
90-91	23.89905819345003	25.0611739555334	25.69800358079129	25.341764270225287
92-93	23.942544156634522	25.27374570121937	25.500945704682564	25.28276443746354
94-95	23.81546413546252	25.313472442779872	25.287173874302223	25.583889547455392
96-97	23.442239906147492	25.1448317808492	25.506627142985465	25.906301170017848
98-99	23.15690097026857	25.151730725940975	25.519766119676078	26.171602184114377
100-101	23.28412008868629	25.36541970506685	25.569278410169055	25.781181796077803
102-103	23.18145120269782	25.205500108585156	25.607048727677817	26.005999961039215
104-105	23.40414939735218	25.243892804409207	25.413624808217268	25.938332990021344
106-107	23.404607624636817	25.113437378021903	25.539986450284886	25.941968547056398
108-109	23.32210800485569	25.059226083647033	25.54436229371881	26.074303617778465
110-111	23.017302271351507	25.60907155323618	25.5693530244884	25.80427315092392
112-113	23.096939208043306	25.606142868223063	25.503581355666228	25.793336568067403
114-115	23.083538659672946	25.656978149429477	25.37151741876833	25.887965772129252
116-117	22.851270869866834	25.4509832074254	25.43832086519512	26.259425057512647
118-119	22.842036758859628	25.124061511999223	25.727413878439197	26.30648785070194
120-121	22.78768332783192	25.180888270806616	25.848164602283894	26.183263799077565
122-123	22.688432639290728	25.36843806503096	25.537307014645172	26.405822281033142
124-125	22.31818537647551	25.317594109407132	25.40161247440037	26.962608039716983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	48.0
2	57.0
3	78.5
4	74.0
5	81.0
6	159.0
7	390.5
8	571.5
9	643.5
10	634.5
11	566.5
12	574.0
13	619.5
14	705.0
15	746.0
16	769.5
17	857.5
18	938.0
19	970.5
20	1035.5
21	1107.5
22	1193.5
23	1335.0
24	1477.0
25	1684.0
26	2115.5
27	2685.5
28	3251.5
29	4071.0
30	5276.0
31	6691.5
32	8734.0
33	11026.5
34	13383.5
35	15861.0
36	18699.5
37	21678.0
38	24084.5
39	26941.5
40	30064.0
41	32621.5
42	35195.0
43	38534.0
44	42112.5
45	45506.0
46	48956.0
47	52972.5
48	57802.5
49	62100.0
50	64962.0
51	66685.0
52	66300.5
53	64740.5
54	63325.5
55	60668.5
56	56968.0
57	52225.0
58	46809.5
59	41069.5
60	35177.0
61	29943.5
62	24853.0
63	19330.5
64	14398.0
65	11001.0
66	8483.0
67	6649.5
68	5212.0
69	3984.0
70	3227.5
71	2584.0
72	2018.5
73	1678.5
74	1289.0
75	978.5
76	757.5
77	567.0
78	414.5
79	272.5
80	204.0
81	180.5
82	153.0
83	126.5
84	118.0
85	99.0
86	83.5
87	86.5
88	86.5
89	82.5
90	86.0
91	74.5
92	63.0
93	66.0
94	61.5
95	54.0
96	42.0
97	28.5
98	21.0
99	12.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.352306514604162
2	0.0
3	0.0
4	0.015656463991215064
5	0.17741587536588865
6	0.04805163602833748
7	0.024675164446984112
8	0.008946550852122894
9	0.007142810760969085
10-11	0.0022727125148538
12-13	0.05833295454791419
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.803740091153809E-4
92-93	3.96822820053838E-4
94-95	1.4429920729230474E-4
96-97	3.246732164076857E-4
98-99	7.214960364615237E-5
100-101	0.0
102-103	0.0
104-105	3.6074801823076184E-5
106-107	1.4429920729230474E-4
108-109	4.689724236999904E-4
110-111	4.328976218769142E-4
112-113	8.297204419307523E-4
114-115	2.8859841458460947E-4
116-117	6.854212346384475E-4
118-119	2.164488109384571E-4
120-121	1.0822440546922855E-4
122-123	0.0016594408838615047
124-125	1.803740091153809E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1386009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.29894201997281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.06755479153935	55.73589940885155
2	8.724822666440533	11.568930241937709
3	2.7854171901465246	5.540106383728801
4	1.3173712943608906	3.4936129225443695
5	0.7804809362936486	2.5872530171513355
6	0.4997563983641048	1.9879992287551334
7	0.3427200915456864	1.5905385634927067
8	0.25414941532294166	1.3479869880724562
9	0.19011432089386845	1.1343940504298184
>10	0.9877467095913165	11.466022002776368
>50	0.03616954248298408	1.604084910024108
>100	0.013044444034687505	1.3604398329903433
>500	1.0869983057829121E-4	0.043023902077593254
>1k	5.434991528914561E-4	0.5397085471676648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2049	0.1478345378709662	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1611	0.11623301147395146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.11341917693175152	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	7.214960364615237E-5
5	0.0	0.0	0.0	0.0	7.214960364615237E-5
6	0.0	0.0	0.0	0.0	1.4429920729230474E-4
7	0.0	0.0	0.0	0.0	1.4429920729230474E-4
8	0.0	0.0	0.0	0.0	1.4429920729230474E-4
9	0.0	0.0	0.0	0.0	1.4429920729230474E-4
10-11	0.0	0.0	0.0	0.0	1.4429920729230474E-4
12-13	0.0	0.0	0.0	0.0	2.525236127615333E-4
14-15	0.0	0.0	0.0	0.0	2.8859841458460947E-4
16-17	0.0	0.0	0.0	0.0	3.607480182307618E-4
18-19	0.0	0.0	0.0	0.0	4.3289762187691423E-4
20-21	0.0	0.0	0.0	3.6074801823076184E-5	4.689724236999904E-4
22-23	0.0	0.0	0.0	1.4429920729230474E-4	5.050472255230666E-4
24-25	0.0	0.0	0.0	5.050472255230666E-4	5.771968291692189E-4
26-27	0.0	0.0	0.0	9.018700455769046E-4	6.493464328153713E-4
28-29	0.0	0.0	0.0	0.0034271061731922375	6.493464328153713E-4
30-31	0.0	0.0	0.0	0.01313122786359973	6.493464328153713E-4
32-33	0.0	0.0	0.0	0.03560582939937619	6.493464328153713E-4
34-35	0.0	0.0	0.0	0.06258978116303718	6.493464328153713E-4
36-37	0.0	0.0	0.0	0.10036009867179795	6.493464328153713E-4
38-39	0.0	0.0	0.0	0.16385174988041204	6.493464328153713E-4
40-41	0.0	0.0	0.0	0.2455972508115027	7.214960364615237E-4
42-43	0.0	0.0	0.0	0.31468049630269357	7.214960364615237E-4
44-45	0.0	0.0	0.0	0.38654150153426137	7.214960364615237E-4
46-47	0.0	0.0	0.0	0.46363335303017517	8.657952437538285E-4
48-49	0.0	0.0	0.0	0.5514394206675426	8.657952437538285E-4
50-51	0.0	3.6074801823076184E-5	0.0	0.6470376454986945	8.657952437538285E-4
52-53	0.0	7.214960364615237E-5	0.0	0.7478306417923692	9.740196492230571E-4
54-55	0.0	7.214960364615237E-5	0.0	0.8571733661181132	0.0010822440546922856
56-57	0.0	7.214960364615237E-5	0.0	0.9616459921977418	0.0010822440546922856
58-59	0.0	7.214960364615237E-5	0.0	1.0827130271159855	0.0010822440546922856
60-61	0.0	7.214960364615237E-5	0.0	1.214638577382975	0.0010822440546922856
62-63	0.0	7.214960364615237E-5	0.0	1.3719968629352335	0.0010822440546922856
64-65	0.0	7.214960364615237E-5	0.0	1.5127607396488767	0.0010822440546922856
66-67	0.0	7.214960364615237E-5	0.0	1.6585029390141046	0.0010822440546922856
68-69	0.0	7.214960364615237E-5	0.0	1.8219939408762857	0.0010822440546922856
70-71	0.0	7.214960364615237E-5	0.0	2.000203461882282	0.0010822440546922856
72-73	0.0	7.214960364615237E-5	0.0	2.162467920482479	0.0012265432619845903
74-75	0.0	7.214960364615237E-5	0.0	2.333570705529329	0.0012265432619845903
76-77	0.0	7.214960364615237E-5	0.0	2.529276505419517	0.0012265432619845903
78-79	0.0	7.214960364615237E-5	0.0	2.7446791471051055	0.0012265432619845903
80-81	0.0	7.214960364615237E-5	0.0	2.956690757419324	0.0012986928656307426
82-83	0.0	7.214960364615237E-5	0.0	3.1888681819526425	0.001370842469276895
84-85	0.0	7.214960364615237E-5	0.0	3.4247973858755607	0.001370842469276895
86-87	0.0	7.214960364615237E-5	0.0	3.686844746318386	0.0014069172710999712
88-89	0.0	7.214960364615237E-5	0.0	3.9748659640738264	0.0015151416765691998
90-91	0.0	7.214960364615237E-5	0.0	4.279986637893405	0.0015151416765691998
92-93	0.0	7.214960364615237E-5	0.0	4.573022253102253	0.0015151416765691998
94-95	0.0	7.214960364615237E-5	0.0	4.874968344361401	0.0015151416765691998
96-97	0.0	7.214960364615237E-5	0.0	5.213313910660032	0.001551216478392276
98-99	0.0	7.214960364615237E-5	0.0	5.594335967515363	0.0015872912802153522
100-101	7.214960364615237E-5	7.214960364615237E-5	0.0	5.995199165373386	0.0015872912802153522
102-103	7.214960364615237E-5	7.214960364615237E-5	0.0	6.4157592050268075	0.0016955156856845806
104-105	7.214960364615237E-5	7.214960364615237E-5	0.0	6.859046369828768	0.001731590487507657
106-107	7.214960364615237E-5	7.214960364615237E-5	0.0	7.333826836622273	0.001731590487507657
108-109	7.214960364615237E-5	7.214960364615237E-5	0.0	7.819177220349941	0.001731590487507657
110-111	7.214960364615237E-5	7.214960364615237E-5	0.0	8.344967456921275	0.001767665289330733
112-113	7.214960364615237E-5	7.214960364615237E-5	0.0	8.867510961328534	0.0018037400911538092
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2005	0.0	68.24837	1
GTATCAA	3385	0.0	48.545074	1
GTACATG	7495	0.0	38.029263	1
TACATGG	7690	0.0	36.345837	2
ACATGGG	7905	0.0	35.43269	3
TCAACGC	4575	0.0	34.779903	4
CATGGGG	6930	0.0	34.39814	4
ATGGGGG	4625	0.0	33.373074	5
TATCAAC	4880	0.0	33.21676	2
CAACGCA	4760	0.0	33.177765	5
ATCAACG	4840	0.0	32.752502	3
AACGCAG	4905	0.0	32.43645	6
TAGCCCG	120	3.656622E-5	29.797386	5
TGGGGGG	3585	0.0	29.420172	6
GTGGTAT	950	0.0	28.304798	1
TGGTATC	940	0.0	27.26144	2
ACGCAGA	6000	0.0	26.216017	7
CGCAGAG	6385	0.0	24.722315	8
GTAAGTA	800	0.0	23.092976	2
AGAGTAC	6405	0.0	22.6917	10-11
>>END_MODULE