FastQCFastQC Report
Mon 15 Aug 2016
SRR2921345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911354
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.059.332051
GTATCAA31150.055.5894361
GTACATG103750.043.761661
TACATGG107200.041.7960132
TCAACGC41400.041.2481844
ACATGGG109900.040.6067473
CAACGCA42650.039.899765
TATCAAC44900.038.2988852
ATCAACG44900.038.033843
AACGCAG45300.037.434326
CATGGGG100500.037.3582534
ATGGGGG63650.034.8685345
ACGCAGA54850.030.6948057
CGCAGAG58750.028.5551838
TGGGGGG50200.027.3798796
GCAGAGT66150.025.3608029
AGAGTAC57750.024.00169810-11
ATGGGGA38200.022.8969295
CAGAGTA61500.021.76433810-11
AGTACAT68550.021.3531652
Produced by FastQC (version 0.11.5)