##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921345_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911354 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.665907518962996 33.0 31.0 34.0 27.0 34.0 2 30.68674771915616 33.0 31.0 34.0 27.0 34.0 3 30.841130946962206 33.0 31.0 34.0 27.0 34.0 4 34.25451329267106 37.0 35.0 37.0 31.0 37.0 5 34.271480845515796 37.0 35.0 37.0 31.0 37.0 6 34.31142948925212 37.0 35.0 37.0 31.0 37.0 7 34.20402709283576 37.0 35.0 37.0 30.0 37.0 8 34.19327398273685 37.0 35.0 37.0 30.0 37.0 9 35.8055640137829 39.0 37.0 39.0 30.0 39.0 10-11 35.82199346641177 39.0 37.0 39.0 30.5 39.0 12-13 35.77341978513661 39.0 37.0 39.0 30.0 39.0 14-15 37.09746336889974 41.0 38.0 41.0 31.0 41.0 16-17 37.08848203943383 41.0 38.0 41.0 31.0 41.0 18-19 37.087011615849285 41.0 38.0 41.0 31.0 41.0 20-21 37.11581658865914 41.0 38.0 41.0 31.0 41.0 22-23 37.06868089323066 41.0 38.0 41.0 30.0 41.0 24-25 37.04824328721943 41.0 38.0 41.0 30.0 41.0 26-27 37.01191275922723 41.0 38.0 41.0 30.0 41.0 28-29 36.94401141808372 40.5 38.0 41.0 30.0 41.0 30-31 36.86632565186774 40.0 38.0 41.0 30.0 41.0 32-33 36.78324214143481 40.0 38.0 41.0 30.0 41.0 34-35 36.67534716227344 40.0 38.0 41.0 30.0 41.0 36-37 36.569148101293635 40.0 37.5 41.0 30.0 41.0 38-39 36.47187517330646 40.0 37.0 41.0 29.0 41.0 40-41 36.36768358974842 40.0 37.0 41.0 29.0 41.0 42-43 36.1828287695529 40.0 36.5 41.0 28.0 41.0 44-45 35.950767361776 40.0 36.0 41.0 27.5 41.0 46-47 35.73826512514165 40.0 35.5 41.0 27.0 41.0 48-49 35.537803829118 40.0 35.0 41.0 26.5 41.0 50-51 35.36830670822883 39.5 35.0 41.0 26.0 41.0 52-53 35.175872444350965 39.0 35.0 41.0 26.0 41.0 54-55 34.96619987715515 39.0 35.0 41.0 26.0 41.0 56-57 34.72622235336834 39.0 35.0 40.5 25.0 41.0 58-59 34.45920614391682 38.0 34.5 40.0 24.0 41.0 60-61 34.17194015342004 38.0 34.0 40.0 23.0 41.0 62-63 33.844751417058276 37.0 34.0 40.0 23.0 41.0 64-65 33.50547229869506 36.5 34.0 39.5 22.0 41.0 66-67 33.171066950444555 36.0 33.5 39.0 22.0 41.0 68-69 32.814255496365405 35.5 33.0 39.0 21.0 40.5 70-71 32.755469682748455 35.0 33.0 38.5 22.0 40.0 72-73 32.46345862671174 35.0 33.0 37.5 20.5 40.0 74-75 32.13442747915875 35.0 33.0 37.0 20.0 39.0 76-77 31.834445372233507 35.0 33.0 36.5 20.0 39.0 78-79 31.545697971176452 35.0 33.0 36.0 18.5 38.0 80-81 31.273660452223922 35.0 33.0 36.0 18.0 37.0 82-83 31.056694102714623 35.0 33.0 35.0 17.5 37.0 84-85 30.842620728551594 35.0 32.5 35.0 14.5 36.0 86-87 30.6758538711301 35.0 32.0 35.0 12.0 36.0 88-89 30.542142899745414 35.0 32.0 35.0 10.0 36.0 90-91 30.42165266088856 35.0 32.0 35.0 7.5 35.5 92-93 30.31290697589248 35.0 32.0 35.0 7.0 35.0 94-95 30.19777628843218 35.0 32.0 35.0 6.5 35.0 96-97 30.11633428449152 35.0 32.0 35.0 2.0 35.0 98-99 30.045519825212914 35.0 32.0 35.0 2.0 35.0 100-101 29.961119708855605 35.0 31.5 35.0 2.0 35.0 102-103 29.13590078028455 33.5 29.5 35.0 2.0 35.0 104-105 29.73303009280332 34.0 31.0 35.0 2.0 35.0 106-107 29.81978534588569 35.0 31.0 35.0 2.0 35.0 108-109 29.769291559805247 35.0 31.0 35.0 2.0 35.0 110-111 29.6958718793065 35.0 31.0 35.0 2.0 35.0 112-113 29.61562536296259 35.0 31.0 35.0 2.0 35.0 114-115 29.499254193623997 34.0 31.0 35.0 2.0 35.0 116-117 29.3699861459468 34.0 31.0 35.0 2.0 35.0 118-119 29.37169802140263 34.5 31.0 35.0 2.0 35.0 120-121 29.54532153646054 35.0 32.0 35.0 2.0 35.0 122-123 29.421546191861893 35.0 32.0 35.0 2.0 35.0 124-125 28.85722084972224 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64039.0 3 9792.0 4 5891.0 5 5215.0 6 5703.0 7 6164.0 8 6243.0 9 6599.0 10 7433.0 11 7923.0 12 7921.0 13 7604.0 14 7782.0 15 7067.0 16 5743.0 17 5305.0 18 5034.0 19 4798.0 20 4878.0 21 5122.0 22 5860.0 23 7549.0 24 10688.0 25 13748.0 26 14926.0 27 15939.0 28 19011.0 29 23147.0 30 28142.0 31 34963.0 32 45707.0 33 63326.0 34 102435.0 35 169161.0 36 355684.0 37 631899.0 38 182833.0 39 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.62216542964907 17.79683189537887 12.337157413105269 25.243845261866788 2 16.038917106500364 19.90294792791105 39.51381872276166 24.544316242826923 3 19.122934157272212 25.281336580950164 28.550057687930536 27.04567157384709 4 13.300001569784783 15.605230522895312 35.56718940499924 35.527578502320665 5 14.199365735816045 38.57971285967755 32.66706467328488 14.553856731221526 6 33.009800196569145 34.78091123092269 17.91955946740019 14.28972910510797 7 29.777118473343954 32.112416638541575 20.5132632691558 17.597201618958668 8 27.323150082255776 35.48326837987517 19.413461900715394 17.780119637153657 9 28.650145668862866 15.99746544550394 18.184905273380274 37.16748361225293 10-11 26.538225782864046 25.727087670151295 26.803686786885855 20.930999760098807 12-13 26.373634710722182 23.14345511386676 26.963439678740077 23.519470496670984 14-15 24.061852023739576 24.196921473452562 24.219210679564405 27.522015823243457 16-17 24.260918178376876 27.161601937137725 24.68260037090453 23.894879513580864 18-19 24.446432682446996 26.745097423662124 25.884713589083525 22.92375630480735 20-21 25.384551391155437 25.67551234713562 25.418822102568317 23.521114159140627 22-23 25.211079247131064 25.199411484322553 25.43417942926327 24.15532983928311 24-25 24.6777491518613 25.319713233893737 25.457503071298067 24.545034542946897 26-27 24.30236214539958 25.599535596992784 25.568744568405005 24.52935768920263 28-29 24.470566506264777 26.13908973279665 25.272480027374883 24.11786373356369 30-31 25.203250118312404 25.637258479840586 25.2380701984326 23.921421203414408 32-33 24.78312680923871 25.613862512900944 25.312376141136646 24.290634536723704 34-35 24.6758132498049 25.91662696659255 25.556016869980752 23.851542913621795 36-37 24.681315622454175 25.98653109510851 25.350679697805116 23.9814735846322 38-39 24.78234492198322 25.99860769815775 25.27051388809315 23.94853349176588 40-41 24.70127766356935 25.70466659306632 25.376081179433275 24.21797456393105 42-43 24.480893044476943 25.75469007934743 25.21333287986961 24.551083996306016 44-45 24.260332893037383 25.778869927996045 25.577872080815627 24.38292509815094 46-47 24.23273801145785 25.568353356749945 25.501248182404684 24.697660449387524 48-49 24.159002845596625 25.89872271247597 25.476448417053486 24.46582602487392 50-51 24.323333743188343 25.82510353199093 25.54120481675557 24.31035790806516 52-53 24.387915590585457 25.72397418242245 25.483373237284802 24.404736989707292 54-55 24.034198793765505 25.46131118223433 25.836670177481913 24.66781984651825 56-57 24.284568038340197 25.611453085500223 25.389341962442032 24.71463691371755 58-59 24.38202479409111 25.778958639801825 25.50593952610935 24.33307703999772 60-61 24.82204788101662 25.66827040926175 25.463143787195712 24.046537922525918 62-63 24.55607540204097 25.731903970599312 25.57585410161319 24.13616652574653 64-65 24.301185873531065 25.417499109895065 25.56454627669301 24.71676873988086 66-67 24.371939223975556 25.72017182202503 25.43408082541543 24.473808128583986 68-69 24.227192900071444 25.685572723959773 25.522824170083098 24.564410205885682 70-71 24.260521900826337 25.424150045244936 25.749929300695584 24.565398753233136 72-73 24.320021263315077 25.968399072241382 25.257302332005942 24.454277332437595 74-75 24.197666123314516 25.889408871054453 25.38992776320206 24.522997242428975 76-77 24.18509049347018 25.33524166254855 25.574663554949446 24.905004289031822 78-79 24.125535597919427 25.449713898692433 25.735906767048682 24.688843736339457 80-81 24.419491665092693 25.728104289281795 25.2698557368575 24.582548308768015 82-83 24.47332952431181 25.717487803806094 25.43366861094194 24.37551406094016 84-85 24.358373642231744 25.84436775528816 25.23811878377661 24.559139818703486 86-87 24.037126053352875 26.028447296842298 25.408764094738512 24.52566255506631 88-89 24.10429741558601 25.58839302765446 25.54064977907302 24.766659777686506 90-91 24.46314879630485 25.329775820721455 25.605850170607305 24.601225212366394 92-93 24.51642305451469 25.545933040515635 25.488586885572705 24.44905701939697 94-95 24.43689139037296 25.41066679363611 25.496501974392288 24.65593984159864 96-97 24.104896670950243 25.51007861128436 25.60999091658748 24.775033801177916 98-99 23.920986822329443 25.534637330864413 25.493040983389974 25.05133486341617 100-101 23.945560718995747 25.613340564416948 25.618625220313902 24.8224734962734 102-103 23.945647246011852 25.579337770381827 25.6699849915409 24.805029992065425 104-105 24.109332881276472 25.63460620824753 25.453704982671155 24.802355927804847 106-107 23.98739126351333 25.47631362152181 25.71006551070013 24.826229604264725 108-109 23.985022163807066 25.39851425055357 25.737904086595815 24.878559499043547 110-111 23.856329271058783 25.997022295114046 25.547070209948863 24.599578223878304 112-113 23.80742596027897 26.084516740182956 25.535636410535595 24.572420889002487 114-115 23.918197573656848 26.095000163500217 25.40364278473562 24.583159478107323 116-117 23.726965939412963 25.863836132475278 25.658426202061424 24.75077172605033 118-119 23.829440288066834 25.67416107136382 25.688282508386408 24.80811613218294 120-121 23.65946445291928 25.75576937113992 25.895292804300663 24.68947337164014 122-123 23.728830408086974 25.897645109755274 25.654854850350844 24.71866963180691 124-125 23.64676478697784 25.84497576814433 25.569617339157556 24.938642105720266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 641.0 1 561.0 2 441.0 3 341.5 4 257.5 5 217.0 6 207.0 7 238.0 8 288.5 9 325.0 10 322.0 11 323.0 12 320.5 13 353.5 14 418.5 15 442.0 16 478.5 17 535.5 18 594.5 19 667.0 20 682.5 21 738.0 22 917.5 23 1113.0 24 1312.5 25 1642.5 26 2162.0 27 2918.5 28 3981.0 29 5408.0 30 7542.5 31 10084.5 32 13397.5 33 17176.0 34 20676.5 35 24822.0 36 29910.0 37 34638.0 38 38083.5 39 41805.5 40 45606.5 41 48608.5 42 52230.5 43 57700.5 44 63508.0 45 68229.5 46 72301.0 47 76313.0 48 81828.5 49 86391.5 50 87439.0 51 87258.5 52 85893.5 53 84448.0 54 83573.5 55 80730.5 56 75456.0 57 68835.0 58 61916.5 59 54445.5 60 46148.0 61 38456.5 62 31801.5 63 24730.0 64 18493.5 65 14298.5 66 11099.0 67 8523.5 68 6589.5 69 5129.0 70 4119.0 71 3229.0 72 2423.5 73 1927.5 74 1482.5 75 1093.5 76 778.5 77 512.0 78 342.5 79 230.5 80 156.5 81 98.5 82 66.5 83 50.0 84 38.0 85 33.0 86 24.5 87 19.5 88 21.0 89 16.5 90 11.0 91 5.5 92 3.5 93 6.0 94 4.5 95 3.5 96 3.5 97 2.0 98 1.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01600959319937594 2 0.011248570385182441 3 0.010934656793037815 4 0.013812198054363555 5 0.008527985919929013 6 0.03013570484588412 7 0.01302741407400199 8 0.01266118154983326 9 0.008894218444097744 10-11 0.007873999269627709 12-13 0.0067491422311094645 14-15 0.006016677182772004 16-17 0.004211674027940402 18-19 0.005964358250747899 20-21 0.0052842121344345416 22-23 0.00549348786253096 24-25 0.005755082522651482 26-27 0.0043424713580006635 28-29 0.005912039318723795 30-31 0.00525805266842249 32-33 0.008187912861772336 34-35 0.0077693614055794995 36-37 0.0071676936873023 38-39 0.006827620629145621 40-41 0.006853780095157674 42-43 0.007272331551350508 44-45 0.007298491017362562 46-47 0.009155813104218266 48-49 0.00813559392974823 50-51 0.005702763590627377 52-53 0.0051272553383622296 54-55 0.006121315046820212 56-57 0.0077693614055794995 58-59 0.0069322584931938306 60-61 0.004996458008301968 62-63 0.005545806794555064 64-65 0.003269933251506524 66-67 0.003871600969783724 68-69 0.006461388104976891 70-71 0.004734863348181446 72-73 0.004237833493952454 74-75 0.0036884847076993584 76-77 0.004368630824012716 78-79 0.0029037007273377932 80-81 0.002877541261325741 82-83 0.005336531066458646 84-85 0.009181972570230318 86-87 0.007089215289266143 88-89 0.004656384950145289 90-91 0.004891820144253759 92-93 0.008030956065700022 94-95 0.006958417959205883 96-97 0.009312769900290578 98-99 0.006722982765097412 100-101 0.008397188589868753 102-103 0.005153414804374281 104-105 0.004708703882169394 106-107 0.007298491017362562 108-109 0.00680146116313357 110-111 0.007873999269627709 112-113 0.0052057337363983856 114-115 0.0021712356790003317 116-117 0.0028252223293016364 118-119 0.15214345432609555 120-121 0.01075154053095345 122-123 0.0017526842228074966 124-125 0.0014126111646508182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1911354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.19160312957843 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2106614395063 49.08218961567249 2 10.73673797109636 13.139964176672073 3 3.5641654074443156 6.542909853015143 4 1.694911918408263 4.148575098033234 5 0.9508337005746228 2.909151922389537 6 0.6025681298955157 2.2123265917859194 7 0.4322229258401194 1.851388961905966 8 0.3057126883642734 1.4965639598450486 9 0.24541118835898484 1.3515393637459097 >10 1.1815219042838407 12.675656136580507 >50 0.05410767395872398 2.234655185872237 >100 0.02063166232550729 2.083652206953664 >500 3.422599620690234E-4 0.13194364532625513 >1k 1.7112998103451147E-4 0.13948328220204098 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.231893202410438E-5 2 0.0 0.0 0.0 0.0 1.0463786404820876E-4 3 0.0 0.0 0.0 0.0 1.0463786404820876E-4 4 0.0 0.0 0.0 5.231893202410438E-5 1.0463786404820876E-4 5 0.0 0.0 0.0 5.231893202410438E-5 1.0463786404820876E-4 6 0.0 0.0 0.0 5.231893202410438E-5 1.5695679607231314E-4 7 0.0 0.0 0.0 5.231893202410438E-5 1.5695679607231314E-4 8 0.0 0.0 0.0 5.231893202410438E-5 1.5695679607231314E-4 9 0.0 0.0 0.0 1.5695679607231314E-4 2.0927572809641753E-4 10-11 0.0 0.0 0.0 1.5695679607231314E-4 2.0927572809641753E-4 12-13 0.0 0.0 0.0 1.5695679607231314E-4 2.615946601205219E-4 14-15 0.0 0.0 0.0 2.0927572809641753E-4 3.139135921446263E-4 16-17 0.0 0.0 0.0 2.0927572809641753E-4 5.755082522651482E-4 18-19 0.0 0.0 0.0 2.0927572809641753E-4 5.755082522651482E-4 20-21 0.0 0.0 0.0 2.3543519410846973E-4 6.539866503013047E-4 22-23 0.0 0.0 0.0 4.708703882169394E-4 7.063055823254091E-4 24-25 0.0 0.0 0.0 6.539866503013047E-4 7.324650483374613E-4 26-27 0.0 0.0 0.0 0.0011510165045302964 7.324650483374613E-4 28-29 0.0 0.0 0.0 0.003191454853470367 7.324650483374613E-4 30-31 0.0 0.0 0.0 0.011667121841375278 7.324650483374613E-4 32-33 0.0 0.0 0.0 0.034844408728053514 7.847839803615656E-4 34-35 0.0 0.0 0.0 0.06184097765249137 7.847839803615656E-4 36-37 0.0 0.0 0.0 0.0979933596811475 7.847839803615656E-4 38-39 0.0 0.0 0.0 0.16412448975961544 8.371029123856701E-4 40-41 0.0 0.0 0.0 0.24707615648383294 8.371029123856701E-4 42-43 0.0 0.0 0.0 0.3200872261234706 8.371029123856701E-4 44-45 0.0 0.0 0.0 0.3956357639662773 8.371029123856701E-4 46-47 2.615946601205219E-5 0.0 0.0 0.4785089522924586 8.371029123856701E-4 48-49 5.231893202410438E-5 0.0 0.0 0.5713488971692319 8.632623783977223E-4 50-51 5.231893202410438E-5 0.0 0.0 0.6718535655875364 8.894218444097745E-4 52-53 5.231893202410438E-5 0.0 0.0 0.7787673031787936 8.894218444097745E-4 54-55 5.231893202410438E-5 0.0 0.0 0.8845300242655207 8.894218444097745E-4 56-57 5.231893202410438E-5 0.0 0.0 0.9948706519043569 8.894218444097745E-4 58-59 5.231893202410438E-5 0.0 0.0 1.1189449991995204 9.155813104218266E-4 60-61 5.231893202410438E-5 0.0 0.0 1.2514950134825886 9.417407764338788E-4 62-63 5.231893202410438E-5 0.0 0.0 1.4061236170798292 9.417407764338788E-4 64-65 5.231893202410438E-5 0.0 0.0 1.547201617282827 9.417407764338788E-4 66-67 5.231893202410438E-5 0.0 0.0 1.701150074763754 9.67900242445931E-4 68-69 5.231893202410438E-5 0.0 0.0 1.8684660193768396 9.940597084579833E-4 70-71 5.231893202410438E-5 0.0 0.0 2.0516084409272173 0.0010202191744700354 72-73 5.231893202410438E-5 0.0 0.0 2.2337829622351486 0.0011510165045302964 74-75 5.231893202410438E-5 0.0 0.0 2.4145710318444413 0.0011510165045302964 76-77 5.231893202410438E-5 0.0 0.0 2.6198966805730386 0.0011510165045302964 78-79 1.0463786404820876E-4 0.0 0.0 2.8522973766241106 0.0011510165045302964 80-81 1.0463786404820876E-4 0.0 0.0 3.0885696736449657 0.0011771759705423487 82-83 1.0463786404820876E-4 0.0 0.0 3.337660109011727 0.0012033354365544007 84-85 1.0463786404820876E-4 0.0 0.0 3.5916423645227415 0.0012033354365544007 86-87 1.0463786404820876E-4 0.0 0.0 3.8655058142029155 0.0012033354365544007 88-89 1.0463786404820876E-4 0.0 0.0 4.1601137204306475 0.0012033354365544007 90-91 1.0463786404820876E-4 0.0 0.0 4.485118926164384 0.0012033354365544007 92-93 1.0463786404820876E-4 0.0 0.0 4.810333407626217 0.0013079733006026095 94-95 1.0463786404820876E-4 0.0 0.0 5.141747682532905 0.0013602922326267138 96-97 1.0463786404820876E-4 0.0 0.0 5.495816055006032 0.0014910895626869748 98-99 1.0463786404820876E-4 0.0 0.0 5.880674118975344 0.0016218868927472356 100-101 1.0463786404820876E-4 0.0 0.0 6.309244650650795 0.0016218868927472356 102-103 1.0463786404820876E-4 0.0 0.0 6.767218422123793 0.001648046358759288 104-105 1.0463786404820876E-4 0.0 0.0 7.2440008496594555 0.0017003652907833923 106-107 1.0463786404820876E-4 0.0 0.0 7.74513774005234 0.0017265247567954446 108-109 1.0463786404820876E-4 0.0 0.0 8.26000835010155 0.0017526842228074969 110-111 1.0463786404820876E-4 0.0 0.0 8.814955262081227 0.001778843688819549 112-113 1.0463786404820876E-4 0.0 0.0 9.381333860708168 0.001778843688819549 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1715 0.0 59.33205 1 GTATCAA 3115 0.0 55.589436 1 GTACATG 10375 0.0 43.76166 1 TACATGG 10720 0.0 41.796013 2 TCAACGC 4140 0.0 41.248184 4 ACATGGG 10990 0.0 40.606747 3 CAACGCA 4265 0.0 39.89976 5 TATCAAC 4490 0.0 38.298885 2 ATCAACG 4490 0.0 38.03384 3 AACGCAG 4530 0.0 37.43432 6 CATGGGG 10050 0.0 37.358253 4 ATGGGGG 6365 0.0 34.868534 5 ACGCAGA 5485 0.0 30.694805 7 CGCAGAG 5875 0.0 28.555183 8 TGGGGGG 5020 0.0 27.379879 6 GCAGAGT 6615 0.0 25.360802 9 AGAGTAC 5775 0.0 24.001698 10-11 ATGGGGA 3820 0.0 22.896929 5 CAGAGTA 6150 0.0 21.764338 10-11 AGTACAT 6855 0.0 21.353165 2 >>END_MODULE