FastQCFastQC Report
Mon 15 Aug 2016
SRR2921345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911354
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.11196251453158337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20580.10767236210560681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20470.10709685385334167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.066.313051
GTATCAA42700.057.1027681
GTACATG100050.042.350061
TACATGG105850.040.2540172
TCAACGC59100.039.5268824
ACATGGG109300.038.5472373
CAACGCA61550.037.7598765
TATCAAC63000.037.7421262
ATCAACG62850.036.9788443
CATGGGG96150.036.5676234
AACGCAG63850.036.2006956
ATGGGGG62300.035.8704835
ACGCAGA77650.029.6857037
CGCAGAG82200.027.748318
TGGGGGG47950.025.8416846
GCAGAGT90350.024.5861369
AGAGTAC82300.024.31231310-11
GTAAGTA10000.023.8371322
GAGTACT38050.022.13464512-13
CAGAGTA87300.021.2838389
Produced by FastQC (version 0.11.5)