##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1911354
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.964178796811055	31.0	30.0	34.0	16.0	34.0
2	29.75290971740452	33.0	31.0	34.0	25.0	34.0
3	29.988631096071163	33.0	31.0	34.0	26.0	34.0
4	32.09450368691514	37.0	33.0	37.0	19.0	37.0
5	31.740436360820652	37.0	33.0	37.0	19.0	37.0
6	31.885425724381772	37.0	33.0	37.0	17.0	37.0
7	32.10227671064596	37.0	35.0	37.0	17.0	37.0
8	32.356317040171525	37.0	35.0	37.0	17.0	37.0
9	34.09805248007434	39.0	37.0	39.0	17.0	39.0
10-11	34.28388671067735	39.0	37.0	39.0	17.0	39.0
12-13	34.306192625751166	39.0	37.0	39.0	17.5	39.0
14-15	35.673836714705914	40.0	37.5	41.0	20.5	41.0
16-17	35.70039877489989	40.0	38.0	41.0	21.5	41.0
18-19	35.730978405884	40.5	38.0	41.0	22.0	41.0
20-21	35.7219123197482	41.0	38.0	41.0	21.0	41.0
22-23	35.66848710390644	40.5	38.0	41.0	20.0	41.0
24-25	35.60054913951052	40.5	37.0	41.0	18.5	41.0
26-27	35.50565776930909	40.0	37.0	41.0	18.0	41.0
28-29	35.42559776995785	40.0	37.0	41.0	17.5	41.0
30-31	35.327003265747734	40.0	37.0	41.0	16.5	41.0
32-33	35.19173606773	40.0	36.0	41.0	16.0	41.0
34-35	35.10374164074264	40.0	36.0	41.0	14.5	41.0
36-37	35.01981919623471	40.0	36.0	41.0	9.5	41.0
38-39	34.922443723140766	40.0	35.5	41.0	9.0	41.0
40-41	34.78723669189486	40.0	35.0	41.0	8.0	41.0
42-43	34.64166057151108	40.0	35.0	41.0	2.0	41.0
44-45	34.487565621020494	40.0	35.0	41.0	2.0	41.0
46-47	34.263860331471825	40.0	35.0	41.0	2.0	41.0
48-49	34.061004921118744	40.0	35.0	41.0	2.0	41.0
50-51	33.87912940250733	40.0	35.0	41.0	2.0	41.0
52-53	33.696954096415425	39.0	35.0	41.0	2.0	41.0
54-55	33.500035838468435	39.0	34.5	41.0	2.0	41.0
56-57	33.26058647430042	39.0	34.0	41.0	2.0	41.0
58-59	33.20013665705045	38.5	34.0	41.0	2.0	41.0
60-61	32.97160180688664	38.0	34.0	41.0	2.0	41.0
62-63	32.688563970881376	37.0	34.0	40.0	2.0	41.0
64-65	32.39744416785169	37.0	34.0	40.0	2.0	41.0
66-67	32.08697970130075	36.0	34.0	39.0	2.0	41.0
68-69	31.758728367429583	35.5	33.0	39.0	2.0	41.0
70-71	31.430319030383696	35.0	33.0	38.5	2.0	40.5
72-73	31.093411006019817	35.0	33.0	37.0	2.0	39.5
74-75	30.765696202796555	35.0	33.0	37.0	2.0	39.0
76-77	30.467656436222697	35.0	32.5	36.0	2.0	39.0
78-79	30.209309212212915	35.0	32.0	36.0	2.0	37.5
80-81	29.977408423557332	35.0	32.0	35.5	2.0	37.0
82-83	29.776367956956168	35.0	32.0	35.0	2.0	37.0
84-85	29.603354219051	35.0	32.0	35.0	2.0	36.0
86-87	29.460442963469873	35.0	32.0	35.0	2.0	36.0
88-89	29.341331590066517	35.0	32.0	35.0	2.0	36.0
90-91	29.237718130707343	35.0	31.0	35.0	2.0	35.5
92-93	29.165969255302784	35.0	31.0	35.0	2.0	35.0
94-95	29.09459105953162	35.0	31.0	35.0	2.0	35.0
96-97	29.031856997709475	35.0	31.0	35.0	2.0	35.0
98-99	28.978068426884818	35.0	31.0	35.0	2.0	35.0
100-101	28.921335608160497	35.0	31.0	35.0	2.0	35.0
102-103	28.257080059476163	34.0	29.5	35.0	2.0	35.0
104-105	28.79176018675766	35.0	31.0	35.0	2.0	35.0
106-107	28.84958280883604	35.0	31.0	35.0	2.0	35.0
108-109	28.918780089925782	35.0	31.5	35.0	2.0	35.0
110-111	29.025019698077905	35.0	32.0	35.0	2.0	35.0
112-113	28.983630714142958	35.0	32.0	35.0	2.0	35.0
114-115	28.922327836706334	35.0	32.0	35.0	2.0	35.0
116-117	28.85203028847613	35.0	32.0	35.0	2.0	35.0
118-119	28.773127583901257	35.0	32.0	35.0	2.0	35.0
120-121	28.674957647824527	35.0	31.0	35.0	2.0	35.0
122-123	28.557222000738744	35.0	31.0	35.0	2.0	35.0
124-125	28.036718996062476	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141856.0
3	8827.0
4	6436.0
5	6007.0
6	6301.0
7	6615.0
8	6951.0
9	7056.0
10	7219.0
11	7213.0
12	8049.0
13	9535.0
14	11271.0
15	9233.0
16	6178.0
17	5484.0
18	4820.0
19	4507.0
20	4217.0
21	4451.0
22	5062.0
23	6302.0
24	8129.0
25	10645.0
26	12195.0
27	14469.0
28	17138.0
29	20359.0
30	24586.0
31	30484.0
32	38880.0
33	52923.0
34	81166.0
35	138424.0
36	301013.0
37	643294.0
38	233799.0
39	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80049976114357	17.94651715829094	11.895890094545148	27.357092986020337
2	15.445490474292045	20.232672754497596	38.26062571349944	26.06121105771092
3	18.24026318515565	24.821723239127863	28.682389552118547	28.25562402359793
4	13.200499630823684	17.004664023388667	31.813160677777365	37.98167566801028
5	14.275778827261012	38.177826437648484	31.946264967670828	15.600129767419677
6	32.6362346832488	34.81436699485472	17.159367492109354	15.390030829787122
7	29.238327438245488	32.01820281718739	19.69137265799123	19.05209708657589
8	27.67569456871283	34.63686946220828	18.547226695884124	19.14020927319476
9	28.219970374959253	15.731043139438086	18.215371220805967	37.83361526479669
10-11	26.485365565260338	25.776873920245443	25.81200681834591	21.925753696148302
12-13	26.544273173356814	23.186902093668426	26.354351332894826	23.914473400079935
14-15	24.03377396337884	23.923224060011908	24.05250414104347	27.99049783556578
16-17	23.880845725072383	26.698717244424635	24.60716329889701	24.81327373160597
18-19	24.20114222692395	25.919819143915777	26.321837817588996	23.557200811571274
20-21	25.006722982765094	25.062494964302793	25.957122542448968	23.973659510483145
22-23	24.809846841558393	24.630262107385654	25.85238004053671	24.707511010519244
24-25	24.33298593562469	24.912339629393614	25.73675520076344	25.017919234218255
26-27	23.986634605625124	25.094252556041425	26.069896000426922	24.849216837906532
28-29	24.210114923766085	25.50121536879092	25.76738793546355	24.521281771979446
30-31	24.783478099818243	25.230307938770107	25.723989381349554	24.262224580062092
32-33	24.42736928899618	25.29884573972168	25.813010044188573	24.460774927093567
34-35	24.46987842126576	25.457084349628587	26.02885179825401	24.044185430851638
36-37	24.581997892593417	25.371281301109054	25.99133912399273	24.055381682304798
38-39	24.438670178313384	25.55931554280369	25.930178292456553	24.071835986426375
40-41	24.318467431988005	25.555627058095986	25.84767133665454	24.278234173261467
42-43	24.24778455482344	25.56875911003404	25.56250699765716	24.620949337485364
44-45	24.011119865812404	25.64307815297428	25.91767406770279	24.42812791351053
46-47	24.028568229642442	25.496114272918568	25.478142719768286	24.997174777670697
48-49	24.129543768448965	25.797994510697652	25.35793997344291	24.714521747410476
50-51	24.143669880095473	25.799930311182546	25.476991703263757	24.579408105458224
52-53	24.25505688637479	25.705442319947014	25.43824953409991	24.601251259578287
54-55	23.863423520708356	25.441284032157306	25.839928134714974	24.855364312419365
56-57	24.014337480131886	25.549505743049167	25.398277870033493	25.037878906785448
58-59	24.084209413850076	25.647656059526387	25.592930456629176	24.67520406999436
60-61	24.52083706105724	25.414392101096915	25.681951119468188	24.38281971837765
62-63	24.220081680316678	25.525517512716117	25.722158218728712	24.532242588238496
64-65	24.023284017508008	25.17987248829887	25.72506191945605	25.071781574737074
66-67	23.95940260150658	25.563396419501565	25.573337016586144	24.90386396240571
68-69	23.858845614156245	25.450204410067418	25.649591860011277	25.041358115765057
70-71	23.889164435264217	25.30677205792334	25.81405642282905	24.990007083983397
72-73	23.926023122875197	25.712453056838243	25.358018451840948	25.003505368445616
74-75	23.736628588947937	25.591177772406365	25.592041034784764	25.080152603860927
76-77	23.706597521966103	25.112459544385814	25.695815636454576	25.48512729719351
78-79	23.6646900574148	25.254662401627325	25.835481025492925	25.245166515464952
80-81	23.95767607674978	25.50785987315798	25.36013736858792	25.174326681504315
82-83	23.964843770437085	25.527662588929108	25.457555220016804	25.049938420617007
84-85	23.812961910771108	25.589608204445646	25.28746637200644	25.309963512776807
86-87	23.476394221060044	25.820884043458197	25.397205331927005	25.305516403554755
88-89	23.468467902858393	25.397519245519145	25.54464008237093	25.589372769251533
90-91	23.739529651816778	25.200068014753967	25.615768959112668	25.444633374316584
92-93	23.7835548523869	25.397468174293746	25.553457559795493	25.265519413523858
94-95	23.708473450083122	25.172489114383662	25.481406490953397	25.63763094457982
96-97	23.39622266391189	25.222918706022508	25.59548318541845	25.78537544464715
98-99	23.277294525242315	25.24053628998082	25.4937860804435	25.98838310433337
100-101	23.27352756213658	25.378396675864334	25.582335872894298	25.765739889104793
102-103	23.223379865791475	25.25539486667567	25.578385793526476	25.942839474006384
104-105	23.272690459224194	25.317575917386314	25.459831093559853	25.94990252982964
106-107	23.257685270784904	25.12861321651544	25.59197656526718	26.021724947432475
108-109	23.205153224401432	25.055471350832686	25.687460204766587	26.0519152199993
110-111	23.001660084055818	25.594331542800397	25.55592934623661	25.848079026907172
112-113	22.992449539995622	25.614771413047542	25.521067543956715	25.87171150300012
114-115	22.97582860282	25.589661757396602	25.450022235592645	25.984487404190755
116-117	22.784162758855945	25.383643856701806	25.625411032975244	26.206782351467005
118-119	22.79096523163346	25.1330015782043	25.688839220665816	26.38719396949642
120-121	22.710573457950183	25.299316923308734	25.807072689907457	26.18303692883362
122-123	22.622514421924883	25.403044797172225	25.582449043229673	26.391991737673216
124-125	22.37538601592328	25.237913990224197	25.47892133973194	26.90777865412058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	84.5
2	118.5
3	138.0
4	105.0
5	135.0
6	255.5
7	643.5
8	933.5
9	974.5
10	868.5
11	698.0
12	717.0
13	762.0
14	835.5
15	909.5
16	955.5
17	1039.5
18	1147.0
19	1203.0
20	1263.0
21	1398.5
22	1523.5
23	1712.5
24	1955.5
25	2257.5
26	2752.5
27	3438.0
28	4244.5
29	5406.5
30	7228.5
31	9510.5
32	12414.5
33	15622.0
34	18852.5
35	22598.5
36	27126.5
37	31791.0
38	35393.0
39	39027.0
40	43159.0
41	46819.0
42	50255.5
43	55312.0
44	61202.5
45	66171.5
46	70378.5
47	74825.0
48	80831.0
49	85935.0
50	87844.0
51	88282.5
52	87489.0
53	85736.0
54	84726.0
55	82345.0
56	77207.5
57	70174.5
58	63369.0
59	56479.0
60	47981.0
61	39901.0
62	32967.0
63	25901.0
64	19424.5
65	14897.0
66	11580.0
67	8995.5
68	7045.0
69	5443.5
70	4323.0
71	3422.5
72	2634.0
73	2175.5
74	1720.0
75	1281.5
76	977.0
77	682.0
78	485.5
79	374.5
80	299.5
81	251.0
82	218.5
83	188.5
84	168.5
85	150.0
86	140.0
87	136.5
88	130.0
89	126.5
90	118.0
91	105.5
92	98.5
93	86.5
94	74.5
95	74.0
96	64.5
97	46.0
98	29.0
99	9.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33714319796332864
2	0.0
3	0.0
4	0.017945393684267803
5	0.17396044898014704
6	0.04520355726882618
7	0.02307264902263003
8	0.008789580580049534
9	0.006958417959205883
10-11	0.0020927572809641753
12-13	0.05726307110038224
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.0927572809641753E-4
92-93	3.139135921446263E-4
94-95	7.847839803615657E-5
96-97	3.6623252416873064E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.5695679607231314E-4
108-109	3.6623252416873064E-4
110-111	2.615946601205219E-4
112-113	7.063055823254091E-4
114-115	2.0927572809641753E-4
116-117	5.755082522651482E-4
118-119	2.3543519410846968E-4
120-121	1.0463786404820876E-4
122-123	0.0014649300966749225
124-125	7.847839803615657E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1911354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.80171153135872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.49084252147524	52.63056938533903
2	9.499850713767431	12.122134696615236
3	3.1601168641705244	6.04862593719569
4	1.4857939479807287	3.7918478745642017
5	0.8591887491635932	2.740885636256225
6	0.5322647829708331	2.037564248484381
7	0.3763166292475531	1.6806751516593956
8	0.2602710645969075	1.3284591506697219
9	0.20936398499819903	1.2022002518319728
>10	1.05731206987267	11.754942696804987
>50	0.049723789665238535	2.1781034196901925
>100	0.01846278037220969	1.9967634958843172
>500	8.201695313811719E-5	0.027053689661411442
>1k	4.100847656905757E-4	0.46017436534322304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2140	0.11196251453158337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2058	0.10767236210560681	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2047	0.10709685385334167	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.231893202410438E-5	0.0
2	0.0	0.0	0.0	5.231893202410438E-5	0.0
3	0.0	0.0	0.0	5.231893202410438E-5	0.0
4	0.0	0.0	0.0	5.231893202410438E-5	0.0
5	0.0	0.0	0.0	5.231893202410438E-5	0.0
6	0.0	0.0	0.0	5.231893202410438E-5	0.0
7	0.0	0.0	0.0	5.231893202410438E-5	0.0
8	0.0	0.0	0.0	5.231893202410438E-5	0.0
9	0.0	0.0	0.0	5.231893202410438E-5	0.0
10-11	0.0	0.0	0.0	5.231893202410438E-5	0.0
12-13	0.0	0.0	0.0	5.231893202410438E-5	7.847839803615657E-5
14-15	0.0	0.0	0.0	1.0463786404820876E-4	2.0927572809641753E-4
16-17	0.0	0.0	0.0	1.0463786404820876E-4	2.0927572809641753E-4
18-19	0.0	0.0	0.0	1.0463786404820876E-4	2.0927572809641753E-4
20-21	0.0	0.0	0.0	1.3079733006026096E-4	2.3543519410846973E-4
22-23	0.0	0.0	0.0	3.139135921446263E-4	2.615946601205219E-4
24-25	0.0	0.0	0.0	4.708703882169394E-4	2.615946601205219E-4
26-27	0.0	0.0	0.0	9.94059708457983E-4	3.139135921446263E-4
28-29	0.0	0.0	0.0	0.003008338591386002	3.139135921446263E-4
30-31	5.231893202410438E-5	0.0	0.0	0.01098697572506192	3.139135921446263E-4
32-33	5.231893202410438E-5	0.0	0.0	0.03322252183530628	3.139135921446263E-4
34-35	5.231893202410438E-5	0.0	0.0	0.05825713080884022	3.139135921446263E-4
36-37	5.231893202410438E-5	0.0	0.0	0.09221211769248397	3.139135921446263E-4
38-39	5.231893202410438E-5	0.0	0.0	0.15392229801491508	3.139135921446263E-4
40-41	5.231893202410438E-5	0.0	0.0	0.23124967954654135	3.139135921446263E-4
42-43	5.231893202410438E-5	0.0	0.0	0.2996043642360337	3.139135921446263E-4
44-45	5.231893202410438E-5	0.0	0.0	0.36929318169214076	3.139135921446263E-4
46-47	5.231893202410438E-5	0.0	0.0	0.44630664963162237	3.923919901807828E-4
48-49	5.231893202410438E-5	0.0	0.0	0.5326590469374066	4.1855145619283505E-4
50-51	5.231893202410438E-5	0.0	0.0	0.626571529920674	4.1855145619283505E-4
52-53	5.231893202410438E-5	0.0	0.0	0.7254543114462313	4.7087038821693947E-4
54-55	5.231893202410438E-5	0.0	0.0	0.8238139036515475	5.231893202410438E-4
56-57	5.231893202410438E-5	0.0	0.0	0.926803721340997	5.231893202410438E-4
58-59	5.231893202410438E-5	0.0	0.0	1.0423239232502195	5.231893202410438E-4
60-61	5.231893202410438E-5	0.0	0.0	1.1665552273414552	5.231893202410438E-4
62-63	5.231893202410438E-5	0.0	0.0	1.3131005559409719	5.755082522651482E-4
64-65	5.231893202410438E-5	0.0	0.0	1.4464876731364258	5.755082522651482E-4
66-67	5.231893202410438E-5	0.0	0.0	1.5902339388726525	6.016677182772004E-4
68-69	5.231893202410438E-5	0.0	0.0	1.7460397184404355	6.278271842892526E-4
70-71	5.231893202410438E-5	0.0	0.0	1.9175673370814614	6.278271842892526E-4
72-73	5.231893202410438E-5	0.0	0.0	2.087394590431704	8.894218444097745E-4
74-75	5.231893202410438E-5	0.0	0.0	2.2570125680538506	9.417407764338788E-4
76-77	5.231893202410438E-5	0.0	0.0	2.45012174615482	9.417407764338788E-4
78-79	5.231893202410438E-5	0.0	0.0	2.667506908714974	9.417407764338788E-4
80-81	5.231893202410438E-5	0.0	0.0	2.8868801906920436	0.0010725381064941397
82-83	5.231893202410438E-5	0.0	0.0	3.119071611015019	0.0013079733006026095
84-85	5.231893202410438E-5	0.0	0.0	3.3565995624044525	0.0013079733006026095
86-87	5.231893202410438E-5	0.0	0.0	3.610607977381479	0.0013602922326267138
88-89	5.231893202410438E-5	0.0	0.0	3.886224111284461	0.0014126111646508182
90-91	5.231893202410438E-5	0.0	0.0	4.191949790567315	0.0014126111646508182
92-93	5.231893202410438E-5	0.0	0.0	4.496001264025398	0.0014126111646508182
94-95	5.231893202410438E-5	0.0	0.0	4.806854198646613	0.0014126111646508182
96-97	5.231893202410438E-5	0.0	0.0	5.135233975495905	0.0014387706306628703
98-99	5.231893202410438E-5	0.0	0.0	5.498615117869322	0.0014649300966749225
100-101	5.231893202410438E-5	0.0	0.0	5.9019417648431425	0.0014649300966749225
102-103	5.231893202410438E-5	0.0	0.0	6.332317299673425	0.0014649300966749225
104-105	5.231893202410438E-5	0.0	0.0	6.779068660227253	0.0014649300966749225
106-107	5.231893202410438E-5	0.0	0.0	7.2493896996579394	0.0014649300966749225
108-109	5.231893202410438E-5	0.0	0.0	7.736165043210207	0.0014910895626869748
110-111	5.231893202410438E-5	0.0	0.0	8.25373007825866	0.001517249028699027
112-113	5.231893202410438E-5	0.0	0.0	8.788011012088813	0.0016218868927472356
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2235	0.0	66.31305	1
GTATCAA	4270	0.0	57.102768	1
GTACATG	10005	0.0	42.35006	1
TACATGG	10585	0.0	40.254017	2
TCAACGC	5910	0.0	39.526882	4
ACATGGG	10930	0.0	38.547237	3
CAACGCA	6155	0.0	37.759876	5
TATCAAC	6300	0.0	37.742126	2
ATCAACG	6285	0.0	36.978844	3
CATGGGG	9615	0.0	36.567623	4
AACGCAG	6385	0.0	36.200695	6
ATGGGGG	6230	0.0	35.870483	5
ACGCAGA	7765	0.0	29.685703	7
CGCAGAG	8220	0.0	27.74831	8
TGGGGGG	4795	0.0	25.841684	6
GCAGAGT	9035	0.0	24.586136	9
AGAGTAC	8230	0.0	24.312313	10-11
GTAAGTA	1000	0.0	23.837132	2
GAGTACT	3805	0.0	22.134645	12-13
CAGAGTA	8730	0.0	21.283838	9
>>END_MODULE