FastQCFastQC Report
Mon 15 Aug 2016
SRR2921344_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921344_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791054
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.053.428561
GGTATCA13850.050.2635271
GTACATG86600.044.5906371
TACATGG90450.042.3613622
TCAACGC30550.041.6768764
CAACGCA31150.040.3011135
ACATGGG93350.040.280553
CATGGGG84200.037.6623764
AACGCAG33900.037.2052766
TATCAAC35250.036.119962
ATCAACG35600.035.2634743
ATGGGGG53450.034.284335
ACGCAGA42250.029.9914177
TGGGGGG37950.029.6290956
CGCAGAG45800.027.6667568
ATGGGGA29950.024.4343555
TATATAC4950.024.0390553
AGAGTAC45100.023.54538310-11
GCAGAGT54200.023.269169
GAGTACA55950.022.3324341
Produced by FastQC (version 0.11.5)