##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921344_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1791054
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.553371925134584	31.0	31.0	34.0	27.0	34.0
2	30.758797333860397	33.0	31.0	34.0	27.0	34.0
3	30.841048901931487	33.0	31.0	34.0	27.0	34.0
4	34.33975915857366	37.0	35.0	37.0	32.0	37.0
5	34.26154264472205	37.0	35.0	37.0	31.0	37.0
6	34.404536658302874	37.0	35.0	37.0	32.0	37.0
7	34.34018907302627	37.0	35.0	37.0	31.0	37.0
8	34.35622488210852	37.0	35.0	37.0	31.0	37.0
9	36.00497751603246	39.0	37.0	39.0	32.0	39.0
10-11	35.98767960095006	39.0	37.0	39.0	31.5	39.0
12-13	35.912925852598526	39.0	37.0	39.0	31.0	39.0
14-15	37.24537311549513	41.0	38.0	41.0	31.0	41.0
16-17	37.224150416458684	41.0	38.0	41.0	31.0	41.0
18-19	37.20551837074706	41.0	38.0	41.0	31.0	41.0
20-21	37.22469060117673	41.0	38.0	41.0	31.0	41.0
22-23	37.157921815869315	41.0	38.0	41.0	31.0	41.0
24-25	37.127599726194745	41.0	38.0	41.0	31.0	41.0
26-27	37.07888539374022	41.0	38.0	41.0	30.0	41.0
28-29	37.007305195711574	40.0	38.0	41.0	30.0	41.0
30-31	36.935436620001404	40.0	38.0	41.0	30.0	41.0
32-33	36.85526232039905	40.0	38.0	41.0	30.0	41.0
34-35	36.74370566158251	40.0	38.0	41.0	30.0	41.0
36-37	36.63992375439267	40.0	38.0	41.0	30.0	41.0
38-39	36.54349785098607	40.0	37.0	41.0	30.0	41.0
40-41	36.44311868877209	40.0	37.0	41.0	29.5	41.0
42-43	36.255633554320525	40.0	37.0	41.0	28.5	41.0
44-45	36.00531865594226	40.0	36.0	41.0	28.0	41.0
46-47	35.78825959462976	40.0	36.0	41.0	27.0	41.0
48-49	35.58311865527226	40.0	35.0	41.0	27.0	41.0
50-51	35.398899474834366	39.0	35.0	41.0	26.0	41.0
52-53	35.1993284401252	39.0	35.0	41.0	26.0	41.0
54-55	34.986650039585626	39.0	35.0	41.0	26.0	41.0
56-57	34.732293666187616	38.5	35.0	40.0	25.5	41.0
58-59	34.4615491771884	38.0	34.0	40.0	25.0	41.0
60-61	34.166217768978484	37.5	34.0	40.0	24.0	41.0
62-63	33.83694433557001	37.0	34.0	40.0	23.0	41.0
64-65	33.48859023792694	36.5	34.0	39.5	22.5	41.0
66-67	33.14484152906613	36.0	33.0	39.0	22.0	41.0
68-69	32.78808064971798	35.0	33.0	39.0	22.0	40.0
70-71	32.73919686396948	35.0	33.0	38.5	22.0	40.0
72-73	32.45278450565979	35.0	33.0	37.0	22.0	39.5
74-75	32.12827865603158	35.0	33.0	37.0	21.0	39.0
76-77	31.83312647189867	35.0	33.0	36.0	20.0	39.0
78-79	31.554351795088255	35.0	33.0	36.0	20.0	37.5
80-81	31.28988656958417	35.0	33.0	35.5	19.5	37.0
82-83	31.079662031407203	35.0	33.0	35.0	18.5	37.0
84-85	30.877512626643306	35.0	32.0	35.0	18.0	36.0
86-87	30.7219444528194	35.0	32.0	35.0	17.5	36.0
88-89	30.592981841976847	35.0	32.0	35.0	16.5	36.0
90-91	30.48051370868773	35.0	32.0	35.0	14.0	35.0
92-93	30.37592668897755	35.0	32.0	35.0	11.0	35.0
94-95	30.266584927087624	35.0	32.0	35.0	9.0	35.0
96-97	30.185525952874677	35.0	32.0	35.0	7.0	35.0
98-99	30.112447754227397	35.0	32.0	35.0	7.0	35.0
100-101	30.02820238809103	35.0	31.5	35.0	6.0	35.0
102-103	29.183367168159087	33.5	29.5	34.5	2.0	35.0
104-105	29.805794520991547	34.0	31.0	35.0	2.0	35.0
106-107	29.897008688738588	35.0	31.0	35.0	2.0	35.0
108-109	29.84190119337552	35.0	31.0	35.0	2.0	35.0
110-111	29.771916145465184	34.5	31.0	35.0	2.0	35.0
112-113	29.6889630351737	34.0	31.0	35.0	2.0	35.0
114-115	29.57188197564116	34.0	31.0	35.0	2.0	35.0
116-117	29.44331215027576	34.0	31.0	35.0	2.0	35.0
118-119	29.447269317396348	34.5	31.0	35.0	2.0	35.0
120-121	29.62873453285049	35.0	32.0	35.0	2.0	35.0
122-123	29.50971885828121	35.0	32.0	35.0	2.0	35.0
124-125	28.929849686274117	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58853.0
3	8923.0
4	5681.0
5	4525.0
6	4832.0
7	5362.0
8	5689.0
9	5974.0
10	6440.0
11	6602.0
12	6710.0
13	6631.0
14	6204.0
15	5622.0
16	5025.0
17	4800.0
18	4662.0
19	4558.0
20	4660.0
21	4821.0
22	5512.0
23	7120.0
24	9296.0
25	12062.0
26	13624.0
27	15596.0
28	19020.0
29	22741.0
30	27755.0
31	34634.0
32	45282.0
33	62767.0
34	99472.0
35	165886.0
36	351781.0
37	582426.0
38	149474.0
39	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.080400898828216	17.312494695037238	11.877755788552003	25.72934861758254
2	16.450271322461077	19.37976098607483	39.01878317543881	25.151184516025282
3	19.564430784900146	24.294916609524407	28.296252286621115	27.84440031895433
4	13.61334749430155	14.983544016823409	35.116632548561064	36.28647594031398
5	14.717373472622938	37.213418750492075	32.86559751900863	15.20361025787636
6	33.800708742554434	33.814057006581976	17.88053024442961	14.504704006433974
7	30.14585044619549	31.12736325019949	20.68765645879204	18.039129844812983
8	27.766833070883727	34.681375012564075	19.311488591818275	18.24030332473392
9	28.48998490141248	15.381351010890636	18.187488274025963	37.941175813670924
10-11	26.84414627200697	24.875119352731293	26.860757938701553	21.419976436560184
12-13	26.737253533414762	22.316244983423488	26.973275170127376	23.973226313034374
14-15	24.26727476072768	23.456979001658894	24.01908259913309	28.25666363848034
16-17	24.794839918603202	26.363590426356083	24.50215091797533	24.339418737065387
18-19	24.827004930910853	25.986475311664726	25.559689929406066	23.626829828018362
20-21	25.683215412503284	25.157268874847393	25.083677119804083	24.07583859284524
22-23	25.52928882733298	24.705375232172386	25.109291669377033	24.6560442711176
24-25	24.968368884139103	24.943549764986113	25.085010368752364	25.003070982122416
26-27	24.6888506044594	25.14862668800693	25.23913576948493	24.92338693804874
28-29	24.873545119782463	25.54994402427755	24.983486463925672	24.593024392014318
30-31	25.449981127482186	25.193638733106866	24.91736293973525	24.439017199675707
32-33	25.06855434233339	25.196784883704698	25.004145925406846	24.730514848555067
34-35	25.095928456802604	25.316122792891687	25.07551992888563	24.512428821420077
36-37	25.06498702213844	25.288976230007293	25.13341498530516	24.512621762549106
38-39	25.151345441713158	25.41796580112299	24.91202924057588	24.518659516587974
40-41	25.109014559749802	25.200419558436245	25.04720311592214	24.64336276589181
42-43	24.896792419802118	25.21201979195719	24.991743114399533	24.899444673841153
44-45	24.68753699191259	25.21033741091231	25.229796552856275	24.872329044318825
46-47	24.791032612564873	25.067124465244785	25.18583642374362	24.956006498446722
48-49	24.75546697258131	25.283102729417138	25.14766905269294	24.813761245308612
50-51	24.640521105853537	25.333808311238577	25.25256698849584	24.77310359441205
52-53	24.81376686064934	25.262630393907205	25.094397302016922	24.829205443426527
54-55	24.599556325103254	24.985691959188163	25.372023020171962	25.042728695536614
56-57	24.656406797377446	25.185518552972034	25.12884396116869	25.029230688481825
58-59	24.735152308710255	25.25775642332078	25.224840655858532	24.782250612110435
60-61	25.061865916235337	25.158657040598108	25.223761425372842	24.555715617793712
62-63	24.915583054130423	25.25366960707977	25.203836040026545	24.62691129876326
64-65	24.81765742767855	24.997473474271594	25.19730630481235	24.987562793237508
66-67	24.801192412801743	25.150552166524616	25.08743067034694	24.960824750326704
68-69	24.62487681084567	25.074862712143585	25.29463495528897	25.005625521721775
70-71	24.63424323630909	24.94170086352237	25.409154827933445	25.014901072235098
72-73	24.718604880995787	25.350519015492573	25.053922781125713	24.876953322385926
74-75	24.598734610820102	25.33274185429794	25.179614297228266	24.88890923765369
76-77	24.67525506251457	24.931231879708484	25.194578953172353	25.198934104604596
78-79	24.48424208754303	25.017908939394363	25.36201741480342	25.13583155825919
80-81	24.80057308542066	25.219080662442973	25.06712704082117	24.913219211315198
82-83	24.817361337556004	25.199221873310968	25.189813553340983	24.79360323579204
84-85	24.749544569382497	25.315465097612215	24.995428244376043	24.93956208862924
86-87	24.453637040784308	25.460178878730318	25.166924074253995	24.919260006231386
88-89	24.448555174075377	25.090858229893513	25.298566089905115	25.162020506125987
90-91	24.737488037182167	24.943298756090286	25.291658244768467	25.027554961959076
92-93	24.770640665024114	25.129759270762808	25.248441785961067	24.851158278252008
94-95	24.780903611548265	24.940149994234854	25.2556834123842	25.023262981832676
96-97	24.48633679465449	24.939994801457267	25.382540992966334	25.191127410921908
98-99	24.375355260762795	25.01680683530083	25.25335885441037	25.354479049526002
100-101	24.419686667234348	25.038423257482588	25.35918242566967	25.182707649613395
102-103	24.34305043029827	25.01045524568571	25.425287009755067	25.22120731426096
104-105	24.432383357561285	25.08504443528122	25.241355897945	25.241216309212493
106-107	24.476556288136653	24.91554657697015	25.42293715883609	25.184959976057105
108-109	24.46589440840498	24.86498695097206	25.44197509908216	25.2271435415408
110-111	24.337741873162255	25.25579060571171	25.345214018732293	25.061253502393743
112-113	24.323766721748598	25.240672853821827	25.329032866853886	25.106527557575692
114-115	24.398813078249567	25.3297632996179	25.294196964354658	24.977226657777873
116-117	24.302431147634344	25.232997544396834	25.32473369715121	25.139837610817604
118-119	24.333970942150213	25.045583764378705	25.419425156645698	25.20102013682539
120-121	24.26098231182054	25.045606656477876	25.580909028470728	25.11250200323086
122-123	24.214910756285864	25.18667409259236	25.415872776584326	25.182542374537448
124-125	24.20234490636957	25.161429366660883	25.350007900477433	25.286217826492113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	248.0
1	210.0
2	153.0
3	124.5
4	107.0
5	98.0
6	93.5
7	112.0
8	133.5
9	138.0
10	143.0
11	148.5
12	169.5
13	191.0
14	199.0
15	231.0
16	266.0
17	293.5
18	326.0
19	342.5
20	373.0
21	437.5
22	511.0
23	598.0
24	704.0
25	862.5
26	1169.0
27	1642.0
28	2222.5
29	3141.5
30	4613.5
31	6494.5
32	9013.5
33	11875.5
34	15022.5
35	18563.0
36	22744.5
37	27285.0
38	31704.0
39	36022.5
40	39955.0
41	43970.5
42	48158.0
43	53077.5
44	58153.5
45	62455.5
46	67360.5
47	72213.5
48	76773.5
49	80820.0
50	82269.5
51	83523.0
52	83651.5
53	82461.5
54	82277.5
55	79913.5
56	75503.5
57	69839.0
58	63520.0
59	56589.5
60	48345.0
61	40856.0
62	33602.5
63	25660.5
64	18985.0
65	14598.5
66	11204.0
67	8528.0
68	6689.0
69	5097.5
70	3949.0
71	3127.5
72	2385.0
73	1906.5
74	1493.0
75	1096.0
76	791.5
77	520.5
78	354.0
79	241.5
80	149.0
81	101.0
82	76.5
83	50.5
84	32.0
85	24.0
86	19.0
87	14.0
88	12.0
89	8.0
90	8.0
91	7.5
92	3.5
93	4.0
94	4.0
95	2.5
96	3.5
97	2.5
98	3.5
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015521586730495005
2	0.01094327697545691
3	0.011278275250215795
4	0.01205993789131986
5	0.008765788189524157
6	0.031210672598369453
7	0.013790762310907432
8	0.013064932715596516
9	0.008821621235317306
10-11	0.00803995859421324
12-13	0.0065045498349016835
14-15	0.005750803716694192
16-17	0.004159561911589489
18-19	0.005974135899866783
20-21	0.005164556735866143
22-23	0.004997057598486701
24-25	0.00535997239614216
26-27	0.004103728865796341
28-29	0.005527471533521602
30-31	0.005024974121383275
32-33	0.007760793365247503
34-35	0.00759329422786806
36-37	0.006672048972281126
38-39	0.006588299403591405
40-41	0.006895381155453716
42-43	0.006951214201246864
44-45	0.0068116315867639944
46-47	0.009184536032972763
48-49	0.007844542933937224
50-51	0.005220389781659291
52-53	0.004829558461107259
54-55	0.005750803716694192
56-57	0.00714662986152288
58-59	0.006644132449384553
60-61	0.004689975846624389
62-63	0.005164556735866143
64-65	0.003182483610209407
66-67	0.003545398407864866
68-69	0.006085801991453077
70-71	0.004215394957382637
72-73	0.003740814068140882
74-75	0.003545398407864866
76-77	0.003992062774210046
78-79	0.0027358192438642274
80-81	0.002624153152277932
82-83	0.004801641938210685
84-85	0.009296202124559057
86-87	0.0066999654951777005
88-89	0.004299144526072357
90-91	0.00491330802979698
92-93	0.007900375979730371
94-95	0.006616215926487979
96-97	0.009352035170352206
98-99	0.00647663331200511
100-101	0.008179541208696108
102-103	0.004857474984003833
104-105	0.004382894094762078
106-107	0.007034963769936585
108-109	0.00647663331200511
110-111	0.00759329422786806
112-113	0.004773725415314112
114-115	0.0019262400798635887
116-117	0.00267998619807108
118-119	0.1598220935828847
120-121	0.010999110021250057
122-123	0.0017587409424841462
124-125	0.0012841600532423926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1791054.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.39929794200192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85475312713268	51.26134583534097
2	10.563110134999091	13.39387533285975
3	3.3740583759718454	6.417387967558384
4	1.6309708391176019	4.136096246557348
5	0.8978549175090308	2.8461685711923295
6	0.562893432060334	2.141222904527348
7	0.419633033365415	1.8623107796047984
8	0.3033202891892502	1.5384234708930764
9	0.22878050443925724	1.3054071027839367
>10	1.111541498951645	12.238417258230717
>50	0.04383967723286978	1.8589437688560366
>100	0.009068148753311517	0.9053765510658486
>500	1.76021277702409E-4	0.09502421052952634
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	5.58330457931475E-5	0.0
7	0.0	0.0	0.0	5.58330457931475E-5	5.58330457931475E-5
8	0.0	0.0	0.0	5.58330457931475E-5	1.11666091586295E-4
9	0.0	0.0	0.0	5.58330457931475E-5	1.11666091586295E-4
10-11	0.0	0.0	0.0	8.374956868972125E-5	1.11666091586295E-4
12-13	0.0	0.0	0.0	1.11666091586295E-4	1.674991373794425E-4
14-15	0.0	0.0	0.0	1.11666091586295E-4	1.9541566027601626E-4
16-17	0.0	0.0	0.0	1.3958261448286874E-4	2.2333218317259E-4
18-19	0.0	0.0	0.0	1.674991373794425E-4	3.34998274758885E-4
20-21	0.0	0.0	0.0	1.674991373794425E-4	4.187478434486062E-4
22-23	0.0	0.0	0.0	2.512487060691637E-4	4.4666436634518E-4
24-25	0.0	0.0	0.0	2.791652289657375E-4	4.4666436634518E-4
26-27	0.0	0.0	0.0	3.629147976554587E-4	4.4666436634518E-4
28-29	0.0	0.0	0.0	0.0013679096219321137	4.4666436634518E-4
30-31	0.0	0.0	0.0	0.008235374254489256	4.4666436634518E-4
32-33	0.0	0.0	0.0	0.025152787129812948	5.024974121383275E-4
34-35	0.0	0.0	0.0	0.04455477054293171	5.024974121383275E-4
36-37	0.0	0.0	0.0	0.07096380120309047	5.024974121383275E-4
38-39	0.0	0.0	0.0	0.12235811985568273	5.024974121383275E-4
40-41	0.0	0.0	0.0	0.18489113114400796	5.024974121383275E-4
42-43	0.0	0.0	0.0	0.24117084130350064	5.024974121383275E-4
44-45	0.0	0.0	0.0	0.30149844728299646	5.58330457931475E-4
46-47	0.0	0.0	0.0	0.36699060999835853	6.141635037246225E-4
48-49	0.0	0.0	0.0	0.43893148950282906	7.258295953109175E-4
50-51	0.0	0.0	0.0	0.5164277570637178	7.258295953109175E-4
52-53	0.0	0.0	0.0	0.5995073292039212	7.537461182074912E-4
54-55	0.0	0.0	0.0	0.6890356181332333	7.816626411040649E-4
56-57	0.0	0.0	0.0	0.7800155662531671	7.816626411040649E-4
58-59	0.0	0.0	0.0	0.8816596261195921	8.095791640006387E-4
60-61	0.0	0.0	0.0	0.9892219888400908	8.374956868972125E-4
62-63	0.0	0.0	0.0	1.1101284495051518	9.491617784835075E-4
64-65	0.0	0.0	0.0	1.2279082596057964	9.770783013800812E-4
66-67	0.0	0.0	0.0	1.3570500945253465	0.001004994824276655
68-69	0.0	0.0	0.0	1.5031651753660136	0.001004994824276655
70-71	0.0	0.0	0.0	1.6619822741246215	0.0010887443929663762
72-73	0.0	0.0	0.0	1.819403546738401	0.0012841600532423926
74-75	0.0	0.0	0.0	1.9753173271157654	0.0013958261448286874
76-77	0.0	0.0	0.0	2.15110767179549	0.0013958261448286874
78-79	0.0	0.0	0.0	2.3548145393717888	0.0013958261448286874
80-81	0.0	0.0	0.0	2.5573209964635346	0.0014795757135184088
82-83	0.0	0.0	0.0	2.774734876782051	0.0016191583280012775
84-85	0.0	0.0	0.0	3.0014449592251267	0.0016470748508978512
86-87	0.0	0.0	0.0	3.2437324614444902	0.001674991373794425
88-89	0.0	0.0	0.0	3.5128198256445646	0.0017587409424841462
90-91	0.0	0.0	0.0	3.8012254236890684	0.00178665746538072
92-93	0.0	0.0	0.0	4.093595167984885	0.001898323556967015
94-95	0.0	0.0	0.0	4.399001928473401	0.001898323556967015
96-97	0.0	0.0	0.0	4.731794797923458	0.0019820731256567363
98-99	0.0	0.0	0.0	5.094709595578916	0.0020658226943464576
100-101	0.0	0.0	0.0	5.482051350768877	0.0020658226943464576
102-103	0.0	0.0	0.0	5.895495054867134	0.002121655740139605
104-105	0.0	0.0	0.0	6.312232908667188	0.0021774887859327525
106-107	0.0	0.0	0.0	6.759539355038989	0.0021774887859327525
108-109	0.0	0.0	0.0	7.229625684094394	0.0022054053088293264
110-111	0.0	0.0	0.0	7.731034351839755	0.0022891548775190473
112-113	0.0	0.0	0.0	8.24137630691202	0.002344987923312195
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2450	0.0	53.42856	1
GGTATCA	1385	0.0	50.263527	1
GTACATG	8660	0.0	44.590637	1
TACATGG	9045	0.0	42.361362	2
TCAACGC	3055	0.0	41.676876	4
CAACGCA	3115	0.0	40.301113	5
ACATGGG	9335	0.0	40.28055	3
CATGGGG	8420	0.0	37.662376	4
AACGCAG	3390	0.0	37.205276	6
TATCAAC	3525	0.0	36.11996	2
ATCAACG	3560	0.0	35.263474	3
ATGGGGG	5345	0.0	34.28433	5
ACGCAGA	4225	0.0	29.991417	7
TGGGGGG	3795	0.0	29.629095	6
CGCAGAG	4580	0.0	27.666756	8
ATGGGGA	2995	0.0	24.434355	5
TATATAC	495	0.0	24.039055	3
AGAGTAC	4510	0.0	23.545383	10-11
GCAGAGT	5420	0.0	23.26916	9
GAGTACA	5595	0.0	22.332434	1
>>END_MODULE