FastQCFastQC Report
Mon 15 Aug 2016
SRR2921344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791054
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.059.587471
GTATCAA29350.051.9328421
GTACATG84400.041.9265631
TACATGG88150.039.6825872
ACATGGG90850.038.306473
TCAACGC40150.036.3634224
CATGGGG81400.036.091654
CAACGCA41450.034.9354175
TATCAAC43900.034.47892
ATCAACG42550.034.032273
AACGCAG43900.033.3826836
ATGGGGG49400.031.2433095
ACGCAGA53800.027.0137797
CGCAGAG57100.025.7640638
TGGGGGG35050.024.9850356
ATGGGGA29800.024.7964575
TACCGTA1552.0918142E-423.0567117
AGAGTAC58050.023.03229910-11
GCAGAGT64050.021.6659729
CAGAGTA62800.020.9591679
Produced by FastQC (version 0.11.5)