##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1791054
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.115363355878717	31.0	30.0	34.0	16.0	34.0
2	29.87643421136381	33.0	31.0	34.0	26.0	34.0
3	30.11330367481941	33.0	31.0	34.0	26.0	34.0
4	32.30837875351609	37.0	35.0	37.0	19.0	37.0
5	31.950578821185736	37.0	33.0	37.0	19.0	37.0
6	32.085554651060214	37.0	35.0	37.0	17.0	37.0
7	32.29133850794002	37.0	35.0	37.0	17.0	37.0
8	32.53323071219516	37.0	35.0	37.0	17.0	37.0
9	34.285301280698405	39.0	37.0	39.0	17.0	39.0
10-11	34.45262370648791	39.0	37.0	39.0	20.0	39.0
12-13	34.47397984650379	39.0	37.0	39.0	22.5	39.0
14-15	35.86263116578283	40.0	38.0	41.0	25.0	41.0
16-17	35.87879734502701	40.0	38.0	41.0	25.0	41.0
18-19	35.8981923493094	40.5	38.0	41.0	25.0	41.0
20-21	35.887356830112324	41.0	38.0	41.0	24.0	41.0
22-23	35.83637623432906	40.5	38.0	41.0	24.0	41.0
24-25	35.7742371251788	40.5	38.0	41.0	23.0	41.0
26-27	35.69116927797822	40.0	37.5	41.0	22.0	41.0
28-29	35.61988164510953	40.0	37.0	41.0	21.5	41.0
30-31	35.53402968308046	40.0	37.0	41.0	20.0	41.0
32-33	35.42510973985151	40.0	37.0	41.0	18.0	41.0
34-35	35.34820725673263	40.0	37.0	41.0	18.0	41.0
36-37	35.27299483990991	40.0	36.5	41.0	16.5	41.0
38-39	35.179864761196484	40.0	36.0	41.0	16.0	41.0
40-41	35.060152290215704	40.0	36.0	41.0	15.0	41.0
42-43	34.9299208734075	40.0	35.5	41.0	11.0	41.0
44-45	34.802669266253275	40.0	35.0	41.0	9.0	41.0
46-47	34.625687444376325	40.0	35.0	41.0	7.5	41.0
48-49	34.44751135364986	40.0	35.0	41.0	2.0	41.0
50-51	34.27292169861992	40.0	35.0	41.0	2.0	41.0
52-53	34.08602811528854	39.0	35.0	41.0	2.0	41.0
54-55	33.881611888865436	39.0	35.0	41.0	2.0	41.0
56-57	33.629157468172366	39.0	34.5	41.0	2.0	41.0
58-59	33.558786055585145	38.5	35.0	41.0	2.0	41.0
60-61	33.315135389552744	37.5	34.0	41.0	2.0	41.0
62-63	33.02276955357013	37.0	34.0	40.0	2.0	41.0
64-65	32.72034092774422	36.5	34.0	40.0	2.0	41.0
66-67	32.39910996541701	36.0	34.0	39.0	2.0	41.0
68-69	32.063650509699876	35.5	34.0	39.0	2.0	41.0
70-71	31.731950292955993	35.0	33.0	38.5	2.0	40.5
72-73	31.393082229793183	35.0	33.0	37.0	2.0	39.5
74-75	31.065394734050454	35.0	33.0	37.0	2.0	39.0
76-77	30.77349342900884	35.0	33.0	36.0	2.0	39.0
78-79	30.51814908986552	35.0	33.0	36.0	2.0	37.0
80-81	30.297922899030404	35.0	32.5	35.5	2.0	37.0
82-83	30.101211353761528	35.0	32.0	35.0	2.0	36.5
84-85	29.932652505172932	35.0	32.0	35.0	2.0	36.0
86-87	29.79711946150144	35.0	32.0	35.0	2.0	36.0
88-89	29.67991361511155	35.0	32.0	35.0	2.0	36.0
90-91	29.58281576099883	35.0	32.0	35.0	2.0	35.0
92-93	29.518666662200022	35.0	32.0	35.0	2.0	35.0
94-95	29.449629380242026	35.0	32.0	35.0	2.0	35.0
96-97	29.385316969784274	35.0	32.0	35.0	2.0	35.0
98-99	29.32770424565647	35.0	31.5	35.0	2.0	35.0
100-101	29.278272458563507	35.0	31.0	35.0	2.0	35.0
102-103	28.58959249693197	34.0	29.5	35.0	2.0	35.0
104-105	29.14427286949472	35.0	31.0	35.0	2.0	35.0
106-107	29.21035965414778	35.0	31.0	35.0	2.0	35.0
108-109	29.28129665548889	35.0	31.5	35.0	2.0	35.0
110-111	29.397238720887252	35.0	32.0	35.0	2.0	35.0
112-113	29.35828540066352	35.0	32.0	35.0	2.0	35.0
114-115	29.299148434385565	35.0	32.0	35.0	2.0	35.0
116-117	29.234648983224403	35.0	32.0	35.0	2.0	35.0
118-119	29.15467261176938	35.0	32.0	35.0	2.0	35.0
120-121	29.059979207773747	35.0	32.0	35.0	2.0	35.0
122-123	28.94482299249492	35.0	32.0	35.0	2.0	35.0
124-125	28.414046142662365	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	126540.0
3	7917.0
4	5820.0
5	5388.0
6	5359.0
7	5724.0
8	5814.0
9	6139.0
10	6312.0
11	6171.0
12	6439.0
13	6859.0
14	7439.0
15	6390.0
16	5058.0
17	4606.0
18	4086.0
19	3782.0
20	3888.0
21	3858.0
22	4617.0
23	5552.0
24	7444.0
25	9648.0
26	11676.0
27	13708.0
28	16329.0
29	19553.0
30	23306.0
31	29360.0
32	38086.0
33	50950.0
34	77342.0
35	131769.0
36	298602.0
37	618396.0
38	200935.0
39	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9228057554763	17.633513626171933	11.251942714163576	28.191737904188198
2	15.619462059770392	19.889070904618176	37.47491700417743	27.016550031434004
3	18.37571619839491	23.96253825959463	28.306516721438886	29.355228820571572
4	13.431231351639324	16.57652536121208	31.317965473972016	38.67427781317658
5	14.703312708841162	37.28908930431213	32.04964420793336	15.95795377891335
6	33.632032496800655	34.10901644334985	16.968652228485308	15.290298831364193
7	30.0034401914891	31.188742531824566	19.56586570520016	19.241951571486172
8	27.923408598776263	34.538440320150805	18.274493468668933	19.263657612404
9	28.166268643215574	15.602091200508339	17.948242619908168	38.283397536367914
10-11	26.702324297206975	25.398398505428073	25.83658591262103	22.062691284743927
12-13	26.696215659979504	22.6572138937996	26.248064618218276	24.398505828002627
14-15	24.18140379910377	23.53262380698739	23.599707211507862	28.686265182400977
16-17	24.47486228779255	26.252279384094507	24.175122581452037	25.097735746660906
18-19	24.709221497509287	25.66111909523666	25.442616470525177	24.187042936728876
20-21	25.47321297961982	24.928142870064217	25.08213041036172	24.51651373995424
22-23	25.253370361809303	24.538316544336464	24.96650017252411	25.24181292133012
24-25	24.74757879996918	24.803104764010463	24.971441397076806	25.477875038943548
26-27	24.595768748457612	24.903464663823648	25.140838858013215	25.35992772970553
28-29	24.731470966257856	25.208368926900025	24.967030586459146	25.093129520382966
30-31	25.175957843817105	25.084447481762133	24.8786189584457	24.860975715975062
32-33	24.942352380218573	25.200747716149262	24.912230452013173	24.944669451618992
34-35	25.014795757135182	25.247982472890264	25.06962380810405	24.667597961870495
36-37	25.106948199216774	25.112419837704504	25.092040775990004	24.688591187088722
38-39	24.908070890101584	25.38848633262872	25.018145739882776	24.685297037386924
40-41	24.858770310666234	25.410707884854393	24.983082587124677	24.747439217354696
42-43	24.748109213904215	25.41690535293743	24.814271373169095	25.020714059989256
44-45	24.608861597696105	25.43304110317165	25.078724594568335	24.879372704563902
46-47	24.57801383989539	25.348733204024	24.846124125794088	25.22712883028652
48-49	24.682840383372024	25.452750168336635	24.735602611646552	25.12880683664479
50-51	24.50802711699368	25.633453821046153	24.975182211144947	24.88333685081522
52-53	24.75631667163581	25.394572134620176	24.84860869633188	25.000502497412135
54-55	24.412580525210295	25.19996605350816	25.239523766452603	25.147929654828943
56-57	24.4402457994008	25.40202584623356	24.905502569995097	25.25222578437054
58-59	24.58789628900078	25.43926648777759	25.00544372196483	24.9673935012568
60-61	24.854052418296714	25.198681893454918	25.168867047001374	24.778398641246998
62-63	24.7482487965187	25.347811958768414	25.063314673929426	24.840624570783458
64-65	24.57572468501787	25.04120478779534	25.01842490511174	25.364645622075045
66-67	24.513777920710375	25.321710009860116	24.947070272588096	25.21744179684141
68-69	24.348456272116863	25.255994514961582	25.08204666079303	25.313502552128526
70-71	24.368193253804744	25.063733421772877	25.203986032805265	25.364087291617114
72-73	24.477235192238762	25.41994825393316	24.87811646103356	25.22470009279452
74-75	24.258425485775415	25.481197105168242	24.976075539877638	25.284301869178705
76-77	24.38873981465662	25.087322883620484	24.95617105905238	25.567766242670515
78-79	24.22221775557856	25.099578237172075	25.15945917878523	25.518744828464136
80-81	24.48742472309601	25.35275318332111	24.794059810591975	25.36576228299091
82-83	24.460764443729783	25.34848195531793	24.930459941464637	25.260293659487655
84-85	24.356356648096593	25.393343807612723	24.731805964532615	25.51849357975807
86-87	24.09771006345984	25.617401820380625	24.897685943584058	25.387202172575478
88-89	24.041011605456898	25.27662482538215	24.996761683343998	25.685601885816954
90-91	24.243761698777675	25.14192074213347	25.027155820390522	25.587161738698335
92-93	24.254006238238397	25.30004039528757	25.01749671491675	25.428456651557283
94-95	24.241787238129056	25.13249181766714	24.940342390570024	25.685378553633782
96-97	24.020288651827517	25.090861480455185	25.014677140594305	25.87417272712299
98-99	23.88668906688464	25.185700710308005	24.928310369201597	25.999299853605756
100-101	23.910808942667277	25.27695982365691	25.027023194163885	25.785208039511932
102-103	23.781080860766902	25.078808344137027	25.13787970658618	26.00223108850989
104-105	23.834903916911497	25.26087990646848	24.93406117291829	25.970155003701734
106-107	23.906761046847276	25.069763390718535	25.009854532582494	26.0136210298517
108-109	23.77596940342257	25.044108204684402	25.107395103430946	26.072527288462076
110-111	23.66426853960846	25.410952957496175	25.02394544430768	25.900833058587686
112-113	23.628560174177498	25.48926004992054	24.997313023419547	25.884866752482417
114-115	23.696743589800853	25.514641674278582	24.928394038810712	25.860220697109852
116-117	23.50461852501999	25.35071645204372	25.013316226030792	26.131348796905502
118-119	23.539650568590258	25.178798499764802	25.071738544794194	26.20981238685075
120-121	23.43945808400368	25.214420012813694	25.17793308683023	26.168188816352394
122-123	23.33775537722212	25.36684259924613	25.046942340902735	26.248459682629015
124-125	23.14906370721581	25.424429092829747	24.884914070102894	26.54159312985154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	35.5
2	64.5
3	72.0
4	50.5
5	73.0
6	138.0
7	294.5
8	429.0
9	468.5
10	444.0
11	383.0
12	386.5
13	424.0
14	462.5
15	499.0
16	540.0
17	592.0
18	628.0
19	650.5
20	700.5
21	767.0
22	876.5
23	984.5
24	1050.0
25	1221.0
26	1553.5
27	1999.5
28	2533.0
29	3368.0
30	4652.0
31	6344.0
32	8665.0
33	11271.5
34	14022.0
35	17130.0
36	20866.0
37	25122.0
38	29235.5
39	33460.0
40	37918.0
41	42054.0
42	45966.0
43	50933.5
44	56119.0
45	60753.5
46	65767.0
47	70479.0
48	75710.0
49	80538.0
50	82869.5
51	84776.5
52	84814.0
53	83498.5
54	83361.0
55	81374.0
56	76835.5
57	70629.5
58	64445.0
59	57747.0
60	49572.5
61	41931.0
62	34556.0
63	26751.5
64	20042.0
65	15386.5
66	11759.0
67	8960.5
68	7078.5
69	5569.0
70	4408.5
71	3470.5
72	2652.5
73	2197.5
74	1711.0
75	1272.0
76	983.5
77	664.0
78	445.0
79	339.5
80	289.0
81	224.5
82	192.0
83	167.5
84	146.0
85	155.0
86	132.0
87	105.5
88	100.0
89	97.0
90	97.5
91	91.5
92	83.0
93	79.5
94	79.5
95	65.0
96	54.0
97	46.0
98	25.0
99	9.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34504822300165156
2	0.0
3	0.0
4	0.019374066890222183
5	0.17933574308758976
6	0.047290589786795935
7	0.025459868881675257
8	0.00826329077738583
9	0.006755798540970848
10-11	0.0022054053088293264
12-13	0.0576476197814248
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	8.374956868972125E-5
92-93	1.9541566027601623E-4
94-95	0.0
96-97	1.9541566027601623E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	0.0
108-109	2.2333218317259E-4
110-111	1.9541566027601623E-4
112-113	4.187478434486063E-4
114-115	1.11666091586295E-4
116-117	3.34998274758885E-4
118-119	8.374956868972125E-5
120-121	8.374956868972125E-5
122-123	0.0015074922364149825
124-125	5.58330457931475E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1791054.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.74666961464504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.16428569833931	54.677748155466645
2	9.091309139784968	11.954465967560903
3	3.0198093061005764	5.956272142422753
4	1.4508165888491564	3.8154543575404722
5	0.8399119639518158	2.761070719966384
6	0.5233036604421255	2.0643283722733687
7	0.37832201193255877	1.7411388628534414
8	0.2722410630136598	1.4319154580399083
9	0.20438491562560943	1.209386476966864
>10	1.0052431611201427	11.479803520310757
>50	0.04094757978643914	1.7665258741244818
>100	0.009000530144332764	0.8975834076816754
>500	4.2438090921619666E-4	0.24430668479232354
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	5.58330457931475E-5
3	0.0	0.0	0.0	0.0	5.58330457931475E-5
4	0.0	0.0	0.0	0.0	5.58330457931475E-5
5	0.0	0.0	0.0	0.0	5.58330457931475E-5
6	0.0	0.0	0.0	0.0	5.58330457931475E-5
7	0.0	0.0	0.0	0.0	5.58330457931475E-5
8	0.0	0.0	0.0	0.0	5.58330457931475E-5
9	0.0	0.0	0.0	0.0	5.58330457931475E-5
10-11	0.0	0.0	0.0	2.791652289657375E-5	5.58330457931475E-5
12-13	0.0	0.0	0.0	5.58330457931475E-5	1.9541566027601626E-4
14-15	5.58330457931475E-5	0.0	0.0	5.58330457931475E-5	2.2333218317259E-4
16-17	5.58330457931475E-5	0.0	0.0	1.3958261448286874E-4	2.791652289657375E-4
18-19	5.58330457931475E-5	0.0	0.0	1.674991373794425E-4	3.9083132055203247E-4
20-21	5.58330457931475E-5	0.0	0.0	1.674991373794425E-4	5.024974121383275E-4
22-23	5.58330457931475E-5	0.0	0.0	2.2333218317259E-4	7.258295953109174E-4
24-25	5.58330457931475E-5	0.0	0.0	2.791652289657375E-4	9.491617784835075E-4
26-27	5.58330457931475E-5	0.0	0.0	3.629147976554587E-4	9.491617784835075E-4
28-29	5.58330457931475E-5	0.0	0.0	0.0014795757135184088	9.491617784835075E-4
30-31	5.58330457931475E-5	0.0	0.0	0.008095791640006388	9.491617784835075E-4
32-33	5.58330457931475E-5	0.0	0.0	0.023840710553673983	0.0010329113471732286
34-35	5.58330457931475E-5	0.0	0.0	0.04220978261961951	0.0010608278700698025
36-37	5.58330457931475E-5	0.0	0.0	0.06733465322653588	0.0010608278700698025
38-39	5.58330457931475E-5	0.0	0.0	0.11574190392919476	0.0010608278700698025
40-41	5.58330457931475E-5	0.0	0.0	0.17489701594703455	0.00111666091586295
42-43	5.58330457931475E-5	0.0	0.0	0.22760341117576577	0.00111666091586295
44-45	5.58330457931475E-5	0.0	0.0	0.2840785369955344	0.00111666091586295
46-47	5.58330457931475E-5	0.0	0.0	0.3448807238642721	0.00111666091586295
48-49	5.58330457931475E-5	0.0	0.0	0.41207579447632514	0.0012004104845526713
50-51	5.58330457931475E-5	0.0	0.0	0.4841841731181751	0.001228327007449245
52-53	5.58330457931475E-5	0.0	0.0	0.5626575189804439	0.0013679096219321137
54-55	5.58330457931475E-5	0.0	0.0	0.646742085944924	0.0015354087593115562
56-57	5.58330457931475E-5	0.0	0.0	0.7318595642565774	0.0015633252822081299
58-59	5.58330457931475E-5	0.0	0.0	0.8275015717002391	0.0015633252822081299
60-61	5.58330457931475E-5	0.0	0.0	0.928168553265284	0.0015633252822081299
62-63	5.58330457931475E-5	0.0	0.0	1.0436033754426166	0.0015633252822081299
64-65	5.58330457931475E-5	0.0	0.0	1.1545436374336004	0.0015633252822081299
66-67	5.58330457931475E-5	0.0	0.0	1.274835934594937	0.0015633252822081299
68-69	5.58330457931475E-5	0.0	0.0	1.4108173176241476	0.0015633252822081299
70-71	5.58330457931475E-5	2.791652289657375E-5	0.0	1.5594728020484028	0.0015633252822081299
72-73	5.58330457931475E-5	5.58330457931475E-5	0.0	1.7055599663661734	0.001674991373794425
74-75	5.58330457931475E-5	5.58330457931475E-5	0.0	1.8504188036764946	0.0017029078966909988
76-77	5.58330457931475E-5	5.58330457931475E-5	0.0	2.013786295667244	0.0017308244195875725
78-79	5.58330457931475E-5	5.58330457931475E-5	0.0	2.204511980096636	0.0017308244195875725
80-81	5.58330457931475E-5	5.58330457931475E-5	0.0	2.3944001688391303	0.0018145739882772936
82-83	5.58330457931475E-5	5.58330457931475E-5	0.0	2.5977441216177732	0.002121655740139605
84-85	5.58330457931475E-5	5.58330457931475E-5	0.0	2.8105238591354587	0.002121655740139605
86-87	5.58330457931475E-5	5.58330457931475E-5	0.0	3.0385460181546735	0.0021495722630361785
88-89	5.58330457931475E-5	5.58330457931475E-5	0.0	3.291413882551838	0.0021774887859327525
90-91	5.58330457931475E-5	5.58330457931475E-5	0.0	3.561059577209844	0.0021774887859327525
92-93	5.58330457931475E-5	5.58330457931475E-5	0.0	3.8344460859359906	0.0022333218317259
94-95	5.58330457931475E-5	5.58330457931475E-5	0.0	4.120674195194561	0.0022891548775190473
96-97	5.58330457931475E-5	5.58330457931475E-5	0.0	4.4351259091015685	0.0023449879233121947
98-99	5.58330457931475E-5	5.58330457931475E-5	0.0	4.777466229382252	0.0024008209691053425
100-101	5.58330457931475E-5	5.58330457931475E-5	0.0	5.143591427170817	0.0024008209691053425
102-103	5.58330457931475E-5	5.58330457931475E-5	0.0	5.533166504192503	0.002484570537795064
104-105	5.58330457931475E-5	5.58330457931475E-5	0.0	5.92396990822164	0.0025124870606916374
106-107	5.58330457931475E-5	5.58330457931475E-5	0.0	6.347128562287904	0.002540403583588211
108-109	5.58330457931475E-5	5.58330457931475E-5	0.0	6.78982878238177	0.0025683201064847848
110-111	5.58330457931475E-5	5.58330457931475E-5	0.0	7.258938033135796	0.002624153152277932
112-113	5.58330457931475E-5	5.58330457931475E-5	0.0	7.740274720918521	0.00267998619807108
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1605	0.0	59.58747	1
GTATCAA	2935	0.0	51.932842	1
GTACATG	8440	0.0	41.926563	1
TACATGG	8815	0.0	39.682587	2
ACATGGG	9085	0.0	38.30647	3
TCAACGC	4015	0.0	36.363422	4
CATGGGG	8140	0.0	36.09165	4
CAACGCA	4145	0.0	34.935417	5
TATCAAC	4390	0.0	34.4789	2
ATCAACG	4255	0.0	34.03227	3
AACGCAG	4390	0.0	33.382683	6
ATGGGGG	4940	0.0	31.243309	5
ACGCAGA	5380	0.0	27.013779	7
CGCAGAG	5710	0.0	25.764063	8
TGGGGGG	3505	0.0	24.985035	6
ATGGGGA	2980	0.0	24.796457	5
TACCGTA	155	2.0918142E-4	23.056711	7
AGAGTAC	5805	0.0	23.032299	10-11
GCAGAGT	6405	0.0	21.665972	9
CAGAGTA	6280	0.0	20.959167	9
>>END_MODULE