FastQCFastQC Report
Mon 15 Aug 2016
SRR2921343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719421
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29040.10678743747290324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.064.303241
GTATCAA46650.056.6466981
TCAACGC60500.042.4936184
CAACGCA61950.041.4029545
GTACATG149250.039.4788171
TATCAAC67300.039.2611732
AACGCAG66700.038.8976
ATCAACG67500.038.52773
TACATGG155900.037.1035542
ACATGGG156400.036.338083
CATGGGG147800.033.218114
ATGGGGG93500.032.7149965
ACGCAGA81700.031.6739967
CGCAGAG86100.030.1238848
TATACCG1802.1969754E-829.7553715
TGGGGGG68550.028.8194756
GCAGAGT99400.025.7939919
AGAGTAC87900.024.63429510-11
CAGAGTA92650.022.15140710-11
GTAAGTA21450.021.3628312
Produced by FastQC (version 0.11.5)