##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921343_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719421
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.959296482596848	33.0	31.0	34.0	28.0	34.0
2	31.059866787819907	33.0	31.0	34.0	28.0	34.0
3	31.23152428402958	34.0	31.0	34.0	28.0	34.0
4	34.650073673771	37.0	35.0	37.0	32.0	37.0
5	34.71639698303426	37.0	35.0	37.0	32.0	37.0
6	34.741282795124405	37.0	35.0	37.0	32.0	37.0
7	34.7162818850042	37.0	35.0	37.0	32.0	37.0
8	34.6635313178798	37.0	35.0	37.0	32.0	37.0
9	36.332857251598774	39.0	38.0	39.0	32.0	39.0
10-11	36.33409906005727	39.0	37.5	39.0	32.0	39.0
12-13	36.26045875206523	39.0	37.0	39.0	32.0	39.0
14-15	37.622352515480316	41.0	38.0	41.0	32.0	41.0
16-17	37.604929689077196	41.0	38.0	41.0	32.0	41.0
18-19	37.61180523354052	41.0	38.5	41.0	32.0	41.0
20-21	37.630687194075506	41.0	39.0	41.0	32.0	41.0
22-23	37.57702797764671	41.0	38.5	41.0	32.0	41.0
24-25	37.54416620302631	41.0	38.0	41.0	32.0	41.0
26-27	37.49176736518545	41.0	38.0	41.0	32.0	41.0
28-29	37.418022807060765	41.0	38.0	41.0	32.0	41.0
30-31	37.347170408700975	41.0	38.0	41.0	31.0	41.0
32-33	37.27025513886963	41.0	38.0	41.0	31.0	41.0
34-35	37.16293523511071	40.0	38.0	41.0	31.0	41.0
36-37	37.07224000991388	40.0	38.0	41.0	30.0	41.0
38-39	36.98373863406953	40.0	38.0	41.0	30.0	41.0
40-41	36.88865718106906	40.0	38.0	41.0	30.0	41.0
42-43	36.739787991634984	40.0	37.0	41.0	30.0	41.0
44-45	36.55718018651764	40.0	37.0	41.0	30.0	41.0
46-47	36.367642413587305	40.0	36.5	41.0	29.5	41.0
48-49	36.18515136126403	40.0	36.0	41.0	29.0	41.0
50-51	36.02740546609003	40.0	35.5	41.0	29.0	41.0
52-53	35.8404625469907	39.5	35.0	41.0	28.0	41.0
54-55	35.63621134793031	39.0	35.0	41.0	28.0	41.0
56-57	35.394746528764756	39.0	35.0	41.0	28.0	41.0
58-59	35.13102366275763	38.5	35.0	40.5	27.0	41.0
60-61	34.8481171175776	38.0	35.0	40.0	27.0	41.0
62-63	34.52166968630455	37.0	34.5	40.0	26.0	41.0
64-65	34.18008539317745	37.0	34.0	40.0	26.0	41.0
66-67	33.835909188022015	36.0	34.0	39.0	26.0	41.0
68-69	33.47597245884326	36.0	34.0	39.0	26.0	40.5
70-71	33.39668958943834	35.5	34.0	39.0	26.0	40.0
72-73	33.08783505753614	35.0	34.0	37.5	26.0	40.0
74-75	32.75368580297056	35.0	34.0	37.0	25.5	39.0
76-77	32.44842192510832	35.0	34.0	36.5	25.0	39.0
78-79	32.15930505059717	35.0	33.5	36.0	25.0	38.0
80-81	31.880596825574266	35.0	33.0	36.0	24.0	37.0
82-83	31.658776997015174	35.0	33.0	35.0	24.0	37.0
84-85	31.44687582393458	35.0	33.0	35.0	23.0	36.0
86-87	31.27959867192318	35.0	33.0	35.0	22.5	36.0
88-89	31.14390839079348	35.0	33.0	35.0	20.5	36.0
90-91	31.01851019022064	35.0	33.0	35.0	20.0	35.5
92-93	30.904067630572833	35.0	33.0	35.0	20.0	35.0
94-95	30.776939098433086	35.0	33.0	35.0	19.0	35.0
96-97	30.680702804016	35.0	33.0	35.0	18.0	35.0
98-99	30.60319880592229	35.0	32.5	35.0	17.5	35.0
100-101	30.508312982800383	35.0	32.0	35.0	16.0	35.0
102-103	29.70324436709138	34.0	30.5	35.0	13.0	35.0
104-105	30.270516407720613	34.5	32.0	35.0	10.5	35.0
106-107	30.328424322677513	35.0	32.0	35.0	9.0	35.0
108-109	30.255064037528577	35.0	32.0	35.0	7.0	35.0
110-111	30.169011896282335	35.0	32.0	35.0	3.5	35.0
112-113	30.07329225596184	35.0	32.0	35.0	2.0	35.0
114-115	29.932554025287	35.0	32.0	35.0	2.0	35.0
116-117	29.795468778096513	35.0	31.5	35.0	2.0	35.0
118-119	29.78854653251556	35.0	31.5	35.0	2.0	35.0
120-121	29.943594426901903	35.0	32.0	35.0	2.0	35.0
122-123	29.80040916798098	35.0	32.0	35.0	2.0	35.0
124-125	29.23896833186182	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73935.0
3	11039.0
4	7179.0
5	6230.0
6	6760.0
7	7282.0
8	7624.0
9	8010.0
10	8638.0
11	9121.0
12	9138.0
13	9235.0
14	8913.0
15	8251.0
16	7256.0
17	6785.0
18	6560.0
19	6420.0
20	6489.0
21	6905.0
22	7982.0
23	10011.0
24	13474.0
25	17287.0
26	19571.0
27	22396.0
28	26948.0
29	32427.0
30	39778.0
31	50264.0
32	64645.0
33	87745.0
34	137647.0
35	231053.0
36	496950.0
37	951808.0
38	287578.0
39	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46681856549395	17.609855401879056	12.628272674945704	25.29505335768128
2	16.31451117002027	19.85271402217822	39.08212531959527	24.750649488206236
3	19.12118417045841	24.611374191450892	28.56044791818373	27.70699371990697
4	13.633687177569048	15.514126001795518	35.36816755320812	35.484019267427314
5	14.580260221120653	37.807600833937386	32.98252504735857	14.629613897583399
6	33.25085000012874	34.196103848809415	17.695783271026354	14.85726288003549
7	29.45370879130983	31.807934164660434	20.66729041943696	18.071066624592778
8	27.353514475697306	34.408835471342826	19.921920972107802	18.315729080852066
9	28.357864247482855	15.417405560700129	18.426466467143825	37.798263724673184
10-11	26.600851615309068	25.264440399524048	26.840849953046604	21.29385803212028
12-13	26.53833091566833	22.68033332334273	27.060962117282468	23.72037364370648
14-15	24.205159492144958	23.806077469057996	24.178663123030127	27.810099915766923
16-17	24.45524912996703	26.72401857320661	24.771985811755883	24.04874648507048
18-19	24.639268882474095	26.480540755127958	25.902124292700755	22.97806606969719
20-21	25.555467909209913	25.29969993691302	25.487600217926815	23.657231935950257
22-23	25.557596141894383	24.76311587482192	25.28562896844806	24.39365901483564
24-25	24.9522478975836	25.0987955511295	25.21344104922971	24.735515502057183
26-27	24.52017267035771	25.347595903409186	25.53282838451498	24.599403041718126
28-29	24.64415221060401	25.854708594718645	25.168783031910873	24.332356162766473
30-31	25.298063463186605	25.395019934640345	25.0863883447767	24.220528257396357
32-33	24.9569585553594	25.364654768355845	25.102241244286194	24.576145431998565
34-35	24.896963991181213	25.550668488284217	25.460347639650706	24.092019880883864
36-37	24.818692778657628	25.777077574538637	25.298409225766083	24.10582042103765
38-39	25.007506766202802	25.71410814193148	25.10703922292236	24.17134586894335
40-41	24.95161731924368	25.435251055776746	25.12815779162046	24.484973833359106
42-43	24.676316418043278	25.435361382079662	25.13383959622044	24.75448260365662
44-45	24.56245031866845	25.44838924457898	25.33605863918707	24.6531017975655
46-47	24.609008170774427	25.315940595242804	25.25419318774209	24.820858046240684
48-49	24.417926222938288	25.597473648750903	25.411167380148086	24.573432748162727
50-51	24.59104140491447	25.409611347521903	25.471779121814453	24.527568125749173
52-53	24.634759445505495	25.397684929721017	25.40127045149442	24.566285173279073
54-55	24.093118101230676	25.103365468596866	25.74106320343773	25.062453226734725
56-57	24.4389252578239	25.26979432561256	25.25988332602114	25.031397090542406
58-59	24.604665171626802	25.446158338402203	25.368470447710255	24.58070604226074
60-61	25.07304342006117	25.23805075841962	25.389580229715808	24.2993255918034
62-63	24.725812101281324	25.18505511155159	25.581284596990173	24.507848190176915
64-65	24.420768968967018	24.95584828025719	25.67084412572056	24.95253862505523
66-67	24.572431874559054	25.388743845620766	25.328673316531237	24.710150963288946
68-69	24.354262201365863	25.274318930566675	25.46532856949263	24.90609029857484
70-71	24.437094056445783	25.062093596765617	25.581955089615565	24.918857257173034
72-73	24.447843206188672	25.525697691052557	25.256675361334995	24.769783741423776
74-75	24.22175498725319	25.42855120283381	25.477846841521234	24.87184696839177
76-77	24.141388455105076	24.904087962715245	25.549918664385924	25.404604917793755
78-79	24.10232761849496	25.07313856805247	25.646516257989806	25.178017555462763
80-81	24.524197793683257	25.26281764045793	25.27248914575786	24.940495420100955
82-83	24.379239769253953	25.36286899426739	25.50781617027974	24.750075066198917
84-85	24.3318028277271	25.427901049778516	25.257571773480265	24.98272434901412
86-87	23.936389006314503	25.643590954817668	25.437299838868967	24.98272019999886
88-89	24.015258453571985	25.172476753492973	25.623699218855585	25.18856557407946
90-91	24.40040488770434	24.855784037826293	25.678706345171364	25.065104729298
92-93	24.485341926107242	25.096407598750215	25.54600939105261	24.872241084089932
94-95	24.4115166346752	25.05490096496617	25.46176478538196	25.071817614976677
96-97	23.9371287434575	25.067980952829274	25.663811846669393	25.33107845704383
98-99	23.750585415284913	24.98237088639459	25.70439686182471	25.562646836495784
100-101	23.86017295328893	25.134824975309595	25.753707882192877	25.251294189208593
102-103	23.76531456748155	25.099994833163585	25.761349893932756	25.37334070542211
104-105	23.883675090465122	25.102894883939868	25.637429760819042	25.37600026477597
106-107	23.88892556220967	24.774828364261815	25.868457436157318	25.467788637371203
108-109	23.86588445778159	24.755182777429948	25.94787664488654	25.431056119901918
110-111	23.607089538996824	25.43921635472629	25.722407357045178	25.231286749231714
112-113	23.56870002675353	25.407853401697334	25.789959980031384	25.23348659151775
114-115	23.687778042342877	25.480303639016345	25.623775556452422	25.208142762188356
116-117	23.396201089789496	25.259434070677273	25.824830174316677	25.519534665216554
118-119	23.419016720738707	25.059761653269142	26.010385883744036	25.510835742248112
120-121	23.378734468637543	25.178561249239177	26.120087749784393	25.322616532338888
122-123	23.40000643533181	25.286330895568355	25.978901304993357	25.334761364106477
124-125	23.215457530934973	25.114828838333537	25.88143923591224	25.78827439481925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	470.0
1	428.5
2	341.0
3	262.0
4	201.5
5	169.0
6	188.0
7	223.0
8	271.5
9	301.5
10	312.5
11	337.5
12	358.0
13	371.0
14	420.0
15	486.5
16	526.0
17	588.5
18	645.5
19	711.5
20	781.5
21	785.0
22	855.5
23	1041.0
24	1218.0
25	1529.5
26	2005.5
27	2730.0
28	3780.0
29	5423.5
30	7973.5
31	11096.0
32	15225.5
33	20016.0
34	25220.5
35	31474.0
36	38204.5
37	44031.5
38	49697.5
39	56425.5
40	62068.0
41	66847.5
42	72898.5
43	81726.0
44	91067.0
45	99093.0
46	105475.5
47	111376.0
48	119225.0
49	125239.0
50	127713.5
51	127627.0
52	125632.5
53	123585.5
54	123170.0
55	121738.5
56	114395.5
57	103434.0
58	92631.0
59	81925.0
60	69324.5
61	57134.0
62	46969.0
63	36458.0
64	26683.0
65	19653.0
66	14958.5
67	11463.0
68	8645.5
69	6270.5
70	4801.5
71	3621.5
72	2622.5
73	1966.0
74	1493.0
75	1116.0
76	789.5
77	498.5
78	304.5
79	219.5
80	155.0
81	104.0
82	76.0
83	66.5
84	60.5
85	38.5
86	23.5
87	27.5
88	24.5
89	16.0
90	11.5
91	10.5
92	11.0
93	9.5
94	7.5
95	6.5
96	4.5
97	3.5
98	3.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01860690198391496
2	0.01312779448272261
3	0.01298070434846241
4	0.01573864436584111
5	0.010002129129693416
6	0.032801099940023996
7	0.014525150758194483
8	0.014010335288283793
9	0.00981826646186817
10-11	0.008659931654569117
12-13	0.00733612044622734
14-15	0.006637442308491403
16-17	0.004688498029543789
18-19	0.0068580775098817
20-21	0.005865219103625367
22-23	0.005846832836842843
24-25	0.006214558172493336
26-27	0.0049826782980641835
28-29	0.006453579640666157
30-31	0.005865219103625367
32-33	0.008807021788829313
34-35	0.008310592585701148
36-37	0.007740618315442882
38-39	0.0076303007147477345
40-41	0.00788770844970308
42-43	0.00788770844970308
44-45	0.00792448098326813
46-47	0.010241150597866238
48-49	0.008917339389524461
50-51	0.006122626838580713
52-53	0.005571038835104973
54-55	0.006839691243099174
56-57	0.008089957384310852
58-59	0.00761191444796521
60-61	0.0054423349676273
62-63	0.006012309237885564
64-65	0.0038427297575476544
66-67	0.004320772693893296
68-69	0.0068580775098817
70-71	0.005111382165541856
72-73	0.004780429363456413
74-75	0.004173682559633098
76-77	0.004872360697369036
78-79	0.00354854948902726
80-81	0.0034014593547670624
82-83	0.005681356435800121
84-85	0.010149219263953613
86-87	0.007556755647617637
88-89	0.00520331349945448
90-91	0.005460721234409825
92-93	0.00871509045491669
94-95	0.007593528181182686
96-97	0.010185991797518662
98-99	0.007262575379097242
100-101	0.009101202057349708
102-103	0.00571812896936517
104-105	0.005184927232671955
106-107	0.007998026050398227
108-109	0.007299347912662291
110-111	0.008512841520308918
112-113	0.005644583902235071
114-115	0.0027027812170311253
116-117	0.003254369220506865
118-119	0.17204029828408327
120-121	0.01198784594220608
122-123	0.0021511932135553856
124-125	0.001746695344339843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2719421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.49876918038239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03251962452998	39.916136979710934
2	12.244827062370101	12.85676699202135
3	4.422893092466153	6.965893307126647
4	2.10231262209122	4.414753003686856
5	1.2051263013370175	3.1633823763550013
6	0.7948299862226237	2.503655759060882
7	0.5439007464797078	1.99878838225331
8	0.41345969075079586	1.736489989609465
9	0.31376064609108584	1.4824842963321205
>10	1.785401424473151	16.975357664420855
>50	0.09837628407053987	3.5284864879694733
>100	0.040701232524245484	3.6415656185960232
>500	0.0017511912901039836	0.653219314049706
>1k	1.400953032032122E-4	0.1630198288073747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2904	0.10678743747290324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.677253356504933E-5	0.0
3	0.0	0.0	0.0	3.677253356504933E-5	0.0
4	0.0	0.0	0.0	3.677253356504933E-5	0.0
5	0.0	0.0	0.0	3.677253356504933E-5	0.0
6	0.0	0.0	0.0	3.677253356504933E-5	3.677253356504933E-5
7	0.0	0.0	0.0	3.677253356504933E-5	3.677253356504933E-5
8	0.0	0.0	0.0	3.677253356504933E-5	3.677253356504933E-5
9	0.0	0.0	0.0	3.677253356504933E-5	1.8386266782524662E-4
10-11	0.0	0.0	0.0	7.354506713009865E-5	1.8386266782524662E-4
12-13	0.0	0.0	0.0	7.354506713009865E-5	1.8386266782524662E-4
14-15	0.0	0.0	0.0	7.354506713009865E-5	3.125665353029193E-4
16-17	0.0	0.0	0.0	7.354506713009865E-5	3.6772533565049325E-4
18-19	0.0	1.8386266782524664E-5	0.0	7.354506713009865E-5	4.228841359980672E-4
20-21	0.0	3.677253356504933E-5	0.0	9.193133391262331E-5	4.412704027805919E-4
22-23	0.0	3.677253356504933E-5	0.0	3.1256653530291925E-4	4.780429363456412E-4
24-25	0.0	3.677253356504933E-5	0.0	5.699742702582646E-4	5.148154699106905E-4
26-27	0.0	3.677253356504933E-5	0.0	8.457682719961344E-4	5.515880034757399E-4
28-29	0.0	3.677253356504933E-5	0.0	0.002610849883118502	5.515880034757399E-4
30-31	0.0	3.677253356504933E-5	0.0	0.010866283668472075	5.515880034757399E-4
32-33	0.0	7.354506713009865E-5	0.0	0.031679537666289996	6.251330706058385E-4
34-35	0.0	7.354506713009865E-5	0.0	0.05740192489504199	6.986781377359371E-4
36-37	0.0	7.354506713009865E-5	0.0	0.09413768592652627	6.986781377359371E-4
38-39	0.0	7.354506713009865E-5	0.0	0.16027308754326747	7.722232048660359E-4
40-41	0.0	7.354506713009865E-5	0.0	0.2387272879043002	7.906094716485605E-4
42-43	0.0	7.354506713009865E-5	0.0	0.313467462375263	8.273820052136098E-4
44-45	0.0	7.354506713009865E-5	0.0	0.3970698174354026	8.457682719961345E-4
46-47	0.0	7.354506713009865E-5	0.0	0.48951596681793663	9.560858726912825E-4
48-49	0.0	7.354506713009865E-5	0.0	0.5850326227531523	0.001029630939821381
50-51	0.0	7.354506713009865E-5	0.0	0.6856238883203447	0.001029630939821381
52-53	0.0	7.354506713009865E-5	0.0	0.7926871197949859	0.001084789740168955
54-55	0.0	7.354506713009865E-5	0.0	0.9085573730584562	0.001139948540516529
56-57	0.0	7.354506713009865E-5	0.0	1.0317453605013713	0.0011583348072990538
58-59	0.0	7.354506713009865E-5	0.0	1.1618098117209508	0.0011767210740815785
60-61	0.0	7.354506713009865E-5	0.0	1.3031634307450006	0.001195107340864103
62-63	0.0	7.354506713009865E-5	0.0	1.4522760543512754	0.001250266141211677
64-65	0.0	7.354506713009865E-5	0.0	1.604238549308842	0.0012870386747767264
66-67	0.0	7.354506713009865E-5	0.0	1.7729877058388532	0.001360583741906825
68-69	0.0	7.354506713009865E-5	0.0	1.9618146656953814	0.001360583741906825
70-71	0.0	7.354506713009865E-5	0.0	2.16382457883498	0.0013973562754718742
72-73	0.0	7.354506713009865E-5	0.0	2.3664044662448367	0.0015076738761670224
74-75	0.0	7.354506713009865E-5	0.0	2.580255135192381	0.0015076738761670224
76-77	0.0	7.354506713009865E-5	0.0	2.8232664232570093	0.0015076738761670224
78-79	0.0	7.354506713009865E-5	0.0	3.08661292238311	0.0015076738761670224
80-81	0.0	7.354506713009865E-5	0.0	3.359869619304992	0.0015996052100796456
82-83	0.0	7.354506713009865E-5	0.0	3.6539947290250385	0.0017650816111223675
84-85	0.0	7.354506713009865E-5	0.0	3.958600010811125	0.0017650816111223675
86-87	0.0	7.354506713009865E-5	0.0	4.279642615100788	0.0017650816111223675
88-89	0.0	7.354506713009865E-5	0.0	4.643911332596167	0.0018018541446874169
90-91	0.0	7.354506713009865E-5	0.0	5.036016858000288	0.0018018541446874169
92-93	0.0	1.2870386747767263E-4	0.0	5.430678074487179	0.0018018541446874169
94-95	0.0	1.470901342601973E-4	0.0	5.842309815214342	0.001857012945034991
96-97	0.0	1.470901342601973E-4	0.0	6.276023462347316	0.0018753992118175156
98-99	0.0	1.470901342601973E-4	0.0	6.755647617636255	0.0018753992118175156
100-101	0.0	1.470901342601973E-4	0.0	7.275500924645357	0.0018753992118175156
102-103	0.0	1.470901342601973E-4	0.0	7.815597511382018	0.0019857168125126637
104-105	0.0	1.470901342601973E-4	0.0	8.380552330808653	0.002022489346077713
106-107	0.0	1.470901342601973E-4	0.0	8.988806808508134	0.002059261879642762
108-109	0.0	1.470901342601973E-4	0.0	9.621239227026635	0.00216957948033791
110-111	0.0	1.470901342601973E-4	0.0	10.284450256139083	0.0022982833478155827
112-113	0.0	1.8386266782524662E-4	0.0	10.964962762293887	0.002353442148163157
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	64.30324	1
GTATCAA	4665	0.0	56.646698	1
TCAACGC	6050	0.0	42.493618	4
CAACGCA	6195	0.0	41.402954	5
GTACATG	14925	0.0	39.478817	1
TATCAAC	6730	0.0	39.261173	2
AACGCAG	6670	0.0	38.897	6
ATCAACG	6750	0.0	38.5277	3
TACATGG	15590	0.0	37.103554	2
ACATGGG	15640	0.0	36.33808	3
CATGGGG	14780	0.0	33.21811	4
ATGGGGG	9350	0.0	32.714996	5
ACGCAGA	8170	0.0	31.673996	7
CGCAGAG	8610	0.0	30.123884	8
TATACCG	180	2.1969754E-8	29.755371	5
TGGGGGG	6855	0.0	28.819475	6
GCAGAGT	9940	0.0	25.793991	9
AGAGTAC	8790	0.0	24.634295	10-11
CAGAGTA	9265	0.0	22.151407	10-11
GTAAGTA	2145	0.0	21.362831	2
>>END_MODULE