FastQCFastQC Report
Mon 15 Aug 2016
SRR2921343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719421
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.070.1957241
GTATCAA50600.054.9216081
GTACATG137100.040.4094661
TCAACGC69650.038.598164
TACATGG142850.038.556972
ACATGGG147900.037.2404563
TATCAAC77850.036.1405332
CAACGCA74100.036.11935
CATGGGG139750.035.871984
ATCAACG75700.035.7495963
ATGGGGG89400.034.871885
AACGCAG77600.034.3220336
GTAAGTA17200.033.9632152
TGGGGGG65350.028.994046
TGTAAGT18800.028.9283961
ACGCAGA91300.028.8375767
CGCAGAG97550.026.619668
TAAGTAC25400.025.5802023
GTGGTAT13850.025.027591
AGAGTAC99550.022.85311310-11
Produced by FastQC (version 0.11.5)