##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719421
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94864127327104	31.0	30.0	34.0	16.0	34.0
2	29.755511559262064	33.0	31.0	34.0	25.0	34.0
3	30.006293986844994	33.0	31.0	34.0	26.0	34.0
4	32.07068710582142	37.0	33.0	37.0	19.0	37.0
5	31.716067501133512	37.0	33.0	37.0	19.0	37.0
6	31.87800086856724	37.0	33.0	37.0	17.0	37.0
7	32.10238576520517	37.0	35.0	37.0	17.0	37.0
8	32.36864795851764	37.0	35.0	37.0	17.0	37.0
9	34.087279608416644	39.0	37.0	39.0	17.0	39.0
10-11	34.307977323114	39.0	37.0	39.0	17.0	39.0
12-13	34.334294506073164	39.0	37.0	39.0	18.0	39.0
14-15	35.735210914382144	40.5	38.0	41.0	21.5	41.0
16-17	35.76780093997951	41.0	38.0	41.0	22.0	41.0
18-19	35.788972358454245	41.0	38.0	41.0	22.0	41.0
20-21	35.77508300480139	41.0	38.0	41.0	22.0	41.0
22-23	35.722887886796485	41.0	38.0	41.0	21.0	41.0
24-25	35.660688433309886	41.0	38.0	41.0	19.0	41.0
26-27	35.57189508354904	40.5	37.0	41.0	18.0	41.0
28-29	35.50500051297684	40.0	37.0	41.0	18.0	41.0
30-31	35.414757773805526	40.0	37.0	41.0	17.0	41.0
32-33	35.30334582251148	40.0	37.0	41.0	16.0	41.0
34-35	35.2200860036015	40.0	36.0	41.0	15.5	41.0
36-37	35.144516240773314	40.0	36.0	41.0	14.5	41.0
38-39	35.05317786396442	40.0	36.0	41.0	9.0	41.0
40-41	34.93087149801373	40.0	35.5	41.0	9.0	41.0
42-43	34.7964671523828	40.0	35.0	41.0	7.5	41.0
44-45	34.669133245643096	40.0	35.0	41.0	3.5	41.0
46-47	34.489565977463585	40.0	35.0	41.0	2.0	41.0
48-49	34.30378157703423	40.0	35.0	41.0	2.0	41.0
50-51	34.1341147619291	40.0	35.0	41.0	2.0	41.0
52-53	33.95236835341052	39.0	35.0	41.0	2.0	41.0
54-55	33.75861038066559	39.0	35.0	41.0	2.0	41.0
56-57	33.52204476614691	39.0	34.5	41.0	2.0	41.0
58-59	33.45530169841301	38.5	35.0	41.0	2.0	41.0
60-61	33.21681343197688	38.0	34.0	41.0	2.0	41.0
62-63	32.93107540171235	37.0	34.0	40.0	2.0	41.0
64-65	32.638533717287615	37.0	34.0	40.0	2.0	41.0
66-67	32.326903962277264	36.0	34.0	39.0	2.0	41.0
68-69	31.996376066817163	35.5	34.0	39.0	2.0	41.0
70-71	31.663100343786418	35.0	33.0	38.5	2.0	40.5
72-73	31.327005454469905	35.0	33.0	37.0	2.0	39.5
74-75	30.99932706263576	35.0	33.0	37.0	2.0	39.0
76-77	30.702426913670227	35.0	33.0	36.0	2.0	39.0
78-79	30.447989847838933	35.0	33.0	36.0	2.0	37.5
80-81	30.221971147534713	35.0	33.0	35.5	2.0	37.0
82-83	30.024928468229085	35.0	32.0	35.0	2.0	37.0
84-85	29.85922370975292	35.0	32.0	35.0	2.0	36.0
86-87	29.7204671509119	35.0	32.0	35.0	2.0	36.0
88-89	29.60448915412509	35.0	32.0	35.0	2.0	36.0
90-91	29.502266475106282	35.0	32.0	35.0	2.0	35.0
92-93	29.437556744615858	35.0	32.0	35.0	2.0	35.0
94-95	29.370657945202304	35.0	32.0	35.0	2.0	35.0
96-97	29.305174152880337	35.0	32.0	35.0	2.0	35.0
98-99	29.25818676107892	35.0	32.0	35.0	2.0	35.0
100-101	29.209389241312763	35.0	31.5	35.0	2.0	35.0
102-103	28.53733386628992	34.0	29.5	35.0	2.0	35.0
104-105	29.07501615233537	35.0	31.0	35.0	2.0	35.0
106-107	29.133684707149058	35.0	31.0	35.0	2.0	35.0
108-109	29.205695624178823	35.0	31.5	35.0	2.0	35.0
110-111	29.312982984245544	35.0	32.0	35.0	2.0	35.0
112-113	29.27550588893739	35.0	32.0	35.0	2.0	35.0
114-115	29.211952286902246	35.0	32.0	35.0	2.0	35.0
116-117	29.149287109278042	35.0	32.0	35.0	2.0	35.0
118-119	29.07023995181327	35.0	32.0	35.0	2.0	35.0
120-121	28.97084581607629	35.0	32.0	35.0	2.0	35.0
122-123	28.85311395330109	35.0	32.0	35.0	2.0	35.0
124-125	28.332098082643327	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	204882.0
3	11234.0
4	8544.0
5	8017.0
6	8190.0
7	8342.0
8	8970.0
9	9109.0
10	9333.0
11	9088.0
12	9765.0
13	10688.0
14	12176.0
15	10584.0
16	7591.0
17	6805.0
18	6215.0
19	5828.0
20	5784.0
21	6049.0
22	6798.0
23	8515.0
24	11046.0
25	14023.0
26	17161.0
27	20182.0
28	23931.0
29	28629.0
30	34456.0
31	42893.0
32	55503.0
33	73779.0
34	113490.0
35	196480.0
36	433961.0
37	938738.0
38	332313.0
39	329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77808677641265	17.868851793469496	12.178437915325844	27.174623514792014
2	15.640498473755995	20.091850434338777	37.86394236125999	26.403708730645235
3	18.382994027037373	24.266967122780915	28.652165295480174	28.697873554701538
4	14.01625994730082	16.710582648111615	31.585411066827323	37.687746337760245
5	14.445306593450322	38.29083749301807	31.89435506096204	15.369500852569573
6	33.473190351484575	34.32815283512153	16.928352834091363	15.270303979302536
7	29.424984560788932	31.859506305708518	19.765794800287047	18.94971433321551
8	27.636014330885768	35.04336997942749	18.51199009106482	18.808625598621926
9	27.960351632315927	16.35364614056172	17.889794219190616	37.79620800793173
10-11	26.926081743476427	25.621228761227183	25.57991371830422	21.872775776992174
12-13	26.758739519420256	23.2238204426679	26.14077716341095	23.876662874500898
14-15	24.481957740268975	24.042029534963508	23.6560098638644	27.82000286090311
16-17	24.364763675797164	26.945460081392326	24.215687824724455	24.47408841808606
18-19	24.665084957422923	26.28998967059532	25.66636427386565	23.37856109811611
20-21	25.471267597036277	25.1445620225776	25.43230709772411	23.951863282662007
22-23	25.43293223079472	24.55881233541993	25.205549269495236	24.802706164290118
24-25	25.005617004502064	25.1279408374062	25.004789622496848	24.861652535594896
26-27	24.472121087540323	25.21935735584891	25.478714035083204	24.82980752152756
28-29	24.59705577032758	25.580298159056653	25.154159653838075	24.66848641677769
30-31	25.1930649943499	25.315701393789343	25.07964011456851	24.411593497292255
32-33	24.82565222523471	25.406106667559015	25.12095405602884	24.64728705117744
34-35	24.97619897765002	25.511202568487924	25.411070959590294	24.101527494271757
36-37	24.99199278081621	25.56375051895238	25.33485988377673	24.10939681645468
38-39	24.844626852554274	25.696848704191076	25.19703642797493	24.261488015279724
40-41	24.776046077455458	25.697106111926033	25.061787049522678	24.465060761095835
42-43	24.661609953001022	25.574230691018418	24.992654686420384	24.771504669560173
44-45	24.44832925832374	25.60081723278595	25.292240517374836	24.658612991515476
46-47	24.491095714859892	25.53003010567323	24.976879269520975	25.0019949099459
48-49	24.41361598663833	25.845501671127785	25.004127716892675	24.736754625341202
50-51	24.48401700214862	25.65538767259648	25.25160686778546	24.608988457469437
52-53	24.6121876678896	25.601699773591513	25.1696041179354	24.61650844058349
54-55	24.008750392087137	25.245778421215398	25.55312325675208	25.19234792994538
56-57	24.285408548363787	25.43456860853836	25.061621573121634	25.21840126997622
58-59	24.479181413984815	25.599291172643003	25.167287448320803	24.754239965051383
60-61	24.967796453730408	25.25059562311242	25.297958646344203	24.48364927681297
62-63	24.560209691695402	25.268981889894942	25.458507527889207	24.712300890520446
64-65	24.26496301970162	25.017439374043228	25.50206459389701	25.215533012358144
66-67	24.35009143490471	25.593977541542852	25.113948888384698	24.94198213516774
68-69	24.15142782231953	25.480313640293282	25.21959637731708	25.148662160070103
70-71	24.276932479377045	25.22742892696644	25.306177307595988	25.189461286060528
72-73	24.344667486203864	25.74296146128165	24.84117023439916	25.071200818115326
74-75	24.047324779796874	25.68366575090801	25.144966520446815	25.124042948848302
76-77	23.965671369015684	25.14184085509379	25.208160119378352	25.684327656512178
78-79	24.026290890597668	25.277770525416994	25.29711287807221	25.398825705913136
80-81	24.364193701526908	25.540804458007788	24.81962152973004	25.27538031073526
82-83	24.195462931263677	25.65435804165666	25.021778533003904	25.12840049407576
84-85	24.145783238417295	25.660351964627765	24.825449976300103	25.368414820654838
86-87	23.740880871332536	25.977901178228745	24.91495432299743	25.36626362744128
88-89	23.75742851143681	25.528595976864192	25.07873918749616	25.635236324202836
90-91	24.116786796581767	25.228275031008486	25.104792681666694	25.550145490743052
92-93	24.121721901646385	25.42378646560359	25.06790070794716	25.38659092480286
94-95	24.039142184415127	25.41526333382241	24.905117600487454	25.64047688127501
96-97	23.61610223388406	25.42767065594891	25.04988671262146	25.906340397545573
98-99	23.431186901768225	25.344149608344868	25.10246208705127	26.122201402835643
100-101	23.551061053069752	25.492117623567662	25.145904220052724	25.810917103309862
102-103	23.44557904053841	25.4223233548612	25.132298382633657	25.999799221966736
104-105	23.529351198168875	25.499513591222833	24.991335470185653	25.97979974042264
106-107	23.548347477291095	25.199362510654854	25.113792730650452	26.138497281403595
108-109	23.459046194369627	25.19789667727742	25.23976235299439	26.103294775358567
110-111	23.239292766100313	25.87434818158267	24.97043937631359	25.91591967600343
112-113	23.16172124092883	25.932481024756026	24.979145204353095	25.92665252996205
114-115	23.241189145240952	25.888522735859965	24.85192130441266	26.01836681448642
116-117	22.924222706233685	25.67979074818995	25.08986347371096	26.306123071865407
118-119	23.007214966887197	25.49208626188743	25.144511699476706	26.356187071748664
120-121	22.983079467739053	25.65429441152482	25.145214895245964	26.21741122549016
122-123	22.949594400615876	25.838590217846374	24.92725798121293	26.28455740032482
124-125	22.595823335025482	25.750964774270674	24.781456035545833	26.871755855158003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	64.5
2	85.5
3	104.0
4	91.5
5	111.5
6	212.0
7	541.0
8	816.5
9	913.5
10	863.0
11	699.5
12	668.5
13	739.0
14	846.0
15	924.0
16	969.0
17	1036.5
18	1134.0
19	1209.0
20	1268.0
21	1392.0
22	1544.0
23	1733.5
24	1983.5
25	2281.5
26	2796.0
27	3505.0
28	4397.0
29	5798.5
30	8089.5
31	10882.0
32	14409.0
33	18762.0
34	23374.0
35	28657.5
36	35002.5
37	41271.0
38	46994.0
39	53208.5
40	59042.5
41	63855.0
42	69582.0
43	78203.5
44	87090.5
45	95872.0
46	102629.0
47	108752.0
48	117768.0
49	124371.0
50	127778.5
51	128514.0
52	127027.0
53	125439.5
54	125053.0
55	123969.5
56	116833.5
57	105176.0
58	94408.0
59	83999.5
60	71185.5
61	59092.5
62	48906.0
63	38065.5
64	28111.5
65	20934.0
66	15759.5
67	12078.5
68	9415.0
69	6895.0
70	5258.0
71	4061.5
72	2955.5
73	2368.0
74	1914.5
75	1443.5
76	1084.5
77	744.5
78	523.0
79	426.5
80	338.5
81	294.0
82	286.0
83	280.0
84	253.0
85	213.0
86	205.5
87	199.5
88	190.5
89	174.0
90	164.0
91	149.5
92	128.0
93	124.0
94	109.5
95	93.5
96	81.0
97	65.0
98	46.0
99	21.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3659602540393709
2	0.0
3	0.0
4	0.02180611240407425
5	0.1939015694885051
6	0.0517389547260244
7	0.02552013829414423
8	0.009524086193347775
9	0.007979639783615704
10-11	0.002666008683466076
12-13	0.056519384089480804
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.4709013426019728E-4
92-93	3.3095280208544393E-4
94-95	7.354506713009864E-5
96-97	3.4933906886796856E-4
98-99	1.838626678252466E-5
100-101	0.0
102-103	0.0
104-105	1.838626678252466E-5
106-107	1.1031760069514798E-4
108-109	3.4933906886796856E-4
110-111	3.4933906886796856E-4
112-113	8.273820052136098E-4
114-115	2.022489346077713E-4
116-117	6.619056041708879E-4
118-119	1.6547640104272197E-4
120-121	1.1031760069514798E-4
122-123	0.0018570129450349907
124-125	9.193133391262331E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2719421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.87366081875117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.227820520758	45.628498525252354
2	10.040901128659986	11.421256102120475
3	3.6124259697631063	6.163556680114654
4	1.7418976294310902	3.9627237982900216
5	1.0194345570021763	2.898948761092781
6	0.6741176672674886	2.300372373605934
7	0.458261657304794	1.8244112644654147
8	0.3456273889517435	1.5725675911129635
9	0.2606271059874477	1.3340535863492533
>10	1.49798238829029	15.366388232929069
>50	0.08483181303902648	3.29132598221145
>100	0.0343235819663385	3.2879658326116514
>500	0.0012304903700604082	0.49249257885137854
>1k	5.181012084190167E-4	0.4554386909924414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.677253356504933E-5
3	0.0	0.0	0.0	0.0	3.677253356504933E-5
4	0.0	0.0	0.0	0.0	3.677253356504933E-5
5	0.0	0.0	0.0	0.0	3.677253356504933E-5
6	0.0	0.0	0.0	0.0	3.677253356504933E-5
7	0.0	0.0	0.0	0.0	3.677253356504933E-5
8	0.0	0.0	0.0	0.0	3.677253356504933E-5
9	0.0	0.0	0.0	0.0	3.677253356504933E-5
10-11	3.677253356504933E-5	0.0	0.0	0.0	3.677253356504933E-5
12-13	3.677253356504933E-5	0.0	0.0	0.0	5.515880034757399E-5
14-15	3.677253356504933E-5	0.0	0.0	0.0	1.2870386747767263E-4
16-17	3.677253356504933E-5	0.0	0.0	0.0	2.390214681728206E-4
18-19	3.677253356504933E-5	0.0	0.0	0.0	2.5740773495534525E-4
20-21	3.677253356504933E-5	0.0	0.0	0.0	2.5740773495534525E-4
22-23	3.677253356504933E-5	0.0	0.0	1.6547640104272194E-4	3.4933906886796856E-4
24-25	3.677253356504933E-5	0.0	0.0	4.228841359980672E-4	4.0449786921554256E-4
26-27	3.677253356504933E-5	0.0	0.0	7.170644045184618E-4	4.0449786921554256E-4
28-29	3.677253356504933E-5	0.0	0.0	0.0024821460156408295	4.228841359980672E-4
30-31	3.677253356504933E-5	0.0	0.0	0.01040662699890896	4.412704027805919E-4
32-33	3.677253356504933E-5	0.0	0.0	0.02941802685203946	5.148154699106905E-4
34-35	3.677253356504933E-5	0.0	0.0	0.053136311001496274	5.148154699106905E-4
36-37	3.677253356504933E-5	3.677253356504933E-5	0.0	0.08727960841664457	5.148154699106905E-4
38-39	3.677253356504933E-5	3.677253356504933E-5	0.0	0.14909423733949248	5.148154699106905E-4
40-41	3.677253356504933E-5	3.677253356504933E-5	0.0	0.22183030873116005	5.148154699106905E-4
42-43	3.677253356504933E-5	3.677253356504933E-5	0.0	0.28956531555798093	5.332017366932152E-4
44-45	3.677253356504933E-5	3.677253356504933E-5	0.0	0.3667508635110195	5.699742702582646E-4
46-47	3.677253356504933E-5	3.677253356504933E-5	0.0	0.4506841713732445	6.067468038233139E-4
48-49	3.677253356504933E-5	3.677253356504933E-5	0.0	0.5386440716608425	6.619056041708879E-4
50-51	3.677253356504933E-5	3.677253356504933E-5	0.0	0.6310166759762464	6.619056041708879E-4
52-53	3.677253356504933E-5	3.677253356504933E-5	0.0	0.730173812734402	7.354506713009865E-4
54-55	3.677253356504933E-5	3.677253356504933E-5	0.0	0.8381011987478217	8.457682719961344E-4
56-57	3.677253356504933E-5	3.677253356504933E-5	0.0	0.9515996235963464	8.825408055611837E-4
58-59	3.677253356504933E-5	3.677253356504933E-5	0.0	1.0727467354264015	8.825408055611837E-4
60-61	3.677253356504933E-5	3.677253356504933E-5	0.0	1.2042085429214526	8.825408055611837E-4
62-63	3.677253356504933E-5	3.677253356504933E-5	0.0	1.344109646869683	9.193133391262331E-4
64-65	3.677253356504933E-5	3.677253356504933E-5	0.0	1.4850587680245169	9.376996059087578E-4
66-67	3.677253356504933E-5	3.677253356504933E-5	0.0	1.6400182244676347	9.560858726912825E-4
68-69	3.677253356504933E-5	3.677253356504933E-5	0.0	1.816507999313089	9.560858726912825E-4
70-71	3.677253356504933E-5	3.677253356504933E-5	0.0	2.0049856201007494	9.928584062563319E-4
72-73	3.677253356504933E-5	3.677253356504933E-5	0.0	2.1920291120793727	0.0012870386747767264
74-75	3.677253356504933E-5	3.677253356504933E-5	0.0	2.389332140922645	0.0012870386747767264
76-77	3.677253356504933E-5	3.677253356504933E-5	0.0	2.614582295275355	0.0012870386747767264
78-79	3.677253356504933E-5	3.677253356504933E-5	0.0	2.8557917291952957	0.0012870386747767264
80-81	3.677253356504933E-5	3.677253356504933E-5	0.0	3.1093383481263106	0.001305424941559251
82-83	3.677253356504933E-5	3.677253356504933E-5	0.0	3.3823192510464546	0.0013238112083417757
84-85	3.677253356504933E-5	3.677253356504933E-5	0.0	3.6640152444215146	0.0013238112083417757
86-87	3.677253356504933E-5	3.677253356504933E-5	0.0	3.960806362825028	0.0013238112083417757
88-89	3.677253356504933E-5	3.677253356504933E-5	0.0	4.298065654416877	0.0013238112083417757
90-91	3.677253356504933E-5	3.677253356504933E-5	0.0	4.6599441572305285	0.0013238112083417757
92-93	3.677253356504933E-5	3.677253356504933E-5	0.0	5.027908514349194	0.0013238112083417757
94-95	3.677253356504933E-5	3.677253356504933E-5	0.0	5.4118321510350915	0.0013238112083417757
96-97	3.677253356504933E-5	3.677253356504933E-5	0.0	5.81302049222978	0.0013605837419068249
98-99	3.677253356504933E-5	5.515880034757399E-5	0.0	6.258666826504612	0.0013973562754718742
100-101	3.677253356504933E-5	7.354506713009865E-5	0.0	6.742685299554575	0.0013973562754718742
102-103	3.677253356504933E-5	7.354506713009865E-5	0.0	7.244593610183933	0.001470901342601973
104-105	3.677253356504933E-5	7.354506713009865E-5	0.0	7.772389784443085	0.0015076738761670224
106-107	3.677253356504933E-5	7.354506713009865E-5	0.0	8.342327282167783	0.0015076738761670224
108-109	3.677253356504933E-5	7.354506713009865E-5	0.0	8.935633724973073	0.0016731502772097443
110-111	3.677253356504933E-5	7.354506713009865E-5	0.0	9.556979224621712	0.0018386266782524662
112-113	3.677253356504933E-5	7.354506713009865E-5	0.0	10.197005171321395	0.001912171745382565
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2750	0.0	70.195724	1
GTATCAA	5060	0.0	54.921608	1
GTACATG	13710	0.0	40.409466	1
TCAACGC	6965	0.0	38.59816	4
TACATGG	14285	0.0	38.55697	2
ACATGGG	14790	0.0	37.240456	3
TATCAAC	7785	0.0	36.140533	2
CAACGCA	7410	0.0	36.1193	5
CATGGGG	13975	0.0	35.87198	4
ATCAACG	7570	0.0	35.749596	3
ATGGGGG	8940	0.0	34.87188	5
AACGCAG	7760	0.0	34.322033	6
GTAAGTA	1720	0.0	33.963215	2
TGGGGGG	6535	0.0	28.99404	6
TGTAAGT	1880	0.0	28.928396	1
ACGCAGA	9130	0.0	28.837576	7
CGCAGAG	9755	0.0	26.61966	8
TAAGTAC	2540	0.0	25.580202	3
GTGGTAT	1385	0.0	25.02759	1
AGAGTAC	9955	0.0	22.853113	10-11
>>END_MODULE