FastQCFastQC Report
Mon 15 Aug 2016
SRR2921342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927008
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.060.803691
GTATCAA34500.054.5077061
TCAACGC42450.041.9163444
CAACGCA43750.040.6697735
TATCAAC47850.038.0565452
ATCAACG47400.037.79013
GTACATG105800.037.742161
AACGCAG47950.037.727016
TACATGG110500.036.1368372
ACATGGG114750.034.279833
CATGGGG103750.031.0312374
ACGCAGA60300.029.697947
ATGGGGG65400.028.8443095
CGCAGAG62400.028.6024078
GCAGAGT72750.024.2866049
TGGGGGG47050.022.8921366
AGAGTAC65350.022.07487510-11
GTATAGG5100.021.0035271
AGTACAT74050.020.8144342
CAGAGTA69400.020.74378410-11
Produced by FastQC (version 0.11.5)