##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921342_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1927008
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44193070293429	31.0	31.0	34.0	27.0	34.0
2	30.444016319600127	31.0	31.0	34.0	27.0	34.0
3	30.555025199687805	33.0	31.0	34.0	26.0	34.0
4	34.00626515302479	37.0	35.0	37.0	30.0	37.0
5	34.00090295421711	37.0	35.0	37.0	30.0	37.0
6	34.16083430893904	37.0	35.0	37.0	30.0	37.0
7	34.01121946561717	37.0	35.0	37.0	30.0	37.0
8	34.0298519777811	37.0	35.0	37.0	30.0	37.0
9	35.67111864610837	39.0	37.0	39.0	30.0	39.0
10-11	35.67446917708696	39.0	37.0	39.0	30.0	39.0
12-13	35.613389773161295	39.0	37.0	39.0	30.0	39.0
14-15	36.91434648948007	41.0	38.0	41.0	30.0	41.0
16-17	36.890580890167556	41.0	38.0	41.0	30.0	41.0
18-19	36.87462454748501	40.5	38.0	41.0	30.0	41.0
20-21	36.92166924060513	41.0	38.0	41.0	30.0	41.0
22-23	36.858268881084044	40.0	38.0	41.0	30.0	41.0
24-25	36.835655067337555	40.5	38.0	41.0	30.0	41.0
26-27	36.80989414678092	40.0	38.0	41.0	30.0	41.0
28-29	36.745695399292586	40.0	38.0	41.0	30.0	41.0
30-31	36.66880028520899	40.0	38.0	41.0	30.0	41.0
32-33	36.59934468357163	40.0	38.0	41.0	30.0	41.0
34-35	36.485043393696344	40.0	37.5	41.0	29.5	41.0
36-37	36.37179087995483	40.0	37.0	41.0	29.0	41.0
38-39	36.27256581187001	40.0	37.0	41.0	28.0	41.0
40-41	36.17427068284097	40.0	37.0	41.0	28.0	41.0
42-43	35.96842851716236	40.0	36.5	41.0	27.0	41.0
44-45	35.69039334553878	40.0	36.0	41.0	26.5	41.0
46-47	35.45985408467428	40.0	35.0	41.0	26.0	41.0
48-49	35.2530827583487	39.5	35.0	41.0	25.0	41.0
50-51	35.072764358009934	39.0	35.0	41.0	24.0	41.0
52-53	34.879599358175994	39.0	35.0	41.0	24.0	41.0
54-55	34.670595036450294	39.0	35.0	40.5	24.0	41.0
56-57	34.42341106004749	38.0	34.0	40.0	23.0	41.0
58-59	34.1552087484847	38.0	34.0	40.0	22.5	41.0
60-61	33.869498984954916	37.5	34.0	40.0	22.0	41.0
62-63	33.54697826890184	37.0	34.0	40.0	21.5	41.0
64-65	33.20214031285806	36.5	33.0	39.5	20.5	41.0
66-67	32.860676499526726	36.0	33.0	39.0	20.0	41.0
68-69	32.51186035553563	35.0	33.0	39.0	19.0	40.0
70-71	32.47019368886896	35.0	33.0	38.5	20.0	40.0
72-73	32.182328511350235	35.0	33.0	37.0	18.5	39.5
74-75	31.858728920689483	35.0	33.0	37.0	18.0	39.0
76-77	31.56073430935419	35.0	33.0	36.5	17.0	39.0
78-79	31.27330945175111	35.0	32.5	36.0	14.0	37.5
80-81	30.99869019744599	35.0	32.0	35.5	11.5	37.0
82-83	30.77793086484332	35.0	32.0	35.0	9.0	37.0
84-85	30.565970924874208	35.0	32.0	35.0	7.0	36.0
86-87	30.395270284295655	35.0	32.0	35.0	7.0	36.0
88-89	30.25497325387336	35.0	32.0	35.0	3.5	36.0
90-91	30.13175269640811	35.0	32.0	35.0	2.0	35.0
92-93	30.022715266361114	35.0	31.5	35.0	2.0	35.0
94-95	29.89757826641093	35.0	31.0	35.0	2.0	35.0
96-97	29.805934640644978	35.0	31.0	35.0	2.0	35.0
98-99	29.72074480230492	35.0	31.0	35.0	2.0	35.0
100-101	29.623809812932798	35.0	31.0	35.0	2.0	35.0
102-103	28.755420838937876	33.5	29.5	34.5	2.0	35.0
104-105	29.367226031235987	34.0	31.0	35.0	2.0	35.0
106-107	29.444546934937478	34.0	31.0	35.0	2.0	35.0
108-109	29.37989152094854	34.0	31.0	35.0	2.0	35.0
110-111	29.29423852936781	34.0	31.0	35.0	2.0	35.0
112-113	29.19918209991863	34.0	31.0	35.0	2.0	35.0
114-115	29.06608820513459	34.0	30.0	35.0	2.0	35.0
116-117	28.9355765518358	34.0	30.0	35.0	2.0	35.0
118-119	28.93401584217606	34.0	30.5	35.0	2.0	35.0
120-121	29.103445600640995	35.0	31.0	35.0	2.0	35.0
122-123	28.97039892932463	35.0	31.0	35.0	2.0	35.0
124-125	28.379132053421678	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74043.0
3	9746.0
4	6180.0
5	5095.0
6	5343.0
7	5851.0
8	6391.0
9	6843.0
10	7237.0
11	7754.0
12	7470.0
13	7727.0
14	7927.0
15	7071.0
16	6077.0
17	5756.0
18	5414.0
19	5111.0
20	5232.0
21	5286.0
22	6113.0
23	8011.0
24	10876.0
25	13980.0
26	15709.0
27	17396.0
28	21091.0
29	25070.0
30	31093.0
31	38859.0
32	50821.0
33	69414.0
34	108395.0
35	177979.0
36	370530.0
37	606668.0
38	157390.0
39	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61769151237579	17.82552640211267	12.512508667832067	25.04427341767947
2	16.309485996151043	20.08614479467042	38.74413781506325	24.86023139411529
3	19.455095600905146	24.870611804272457	28.47168303265586	27.20260956216654
4	13.470784028467284	15.573495240314672	35.457151413580604	35.49856931763743
5	14.431217095975951	37.77241833158953	32.68495798249547	15.11140658993905
6	32.945291560831194	34.590588676240266	17.901729654022475	14.562390108906065
7	29.48435084135345	31.674968352902894	20.526937791071546	18.313743014672106
8	28.05161291661974	34.23936032452963	19.406122403433386	18.302904355417244
9	28.3310255633104	15.697853047294409	18.47013531074971	37.50098607864548
10-11	26.516091782519286	25.46275957365372	26.748934458666078	21.272214185160916
12-13	26.34311363634005	22.83913359828862	27.02053489967647	23.797217865694865
14-15	24.175792809315773	23.93205707458993	24.206386275859135	27.685763840235154
16-17	24.535175729444692	26.860633417941983	24.62251759422205	23.981673258391282
18-19	24.509615412061102	26.468117768590215	25.83390615038141	23.188360668967274
20-21	25.527039342029877	25.396752082105106	25.453086207039714	23.623122368825303
22-23	25.44984218157889	24.89654356380047	25.335227915540937	24.318386339079705
24-25	24.808610335755453	25.25453534406546	25.347119599500438	24.589734720678646
26-27	24.470365508453003	25.417369753832347	25.51246978011784	24.599794957596803
28-29	24.574021355175823	25.847422772436257	25.332862106823622	24.24569376556429
30-31	25.210559174795904	25.458341485489854	25.22768519061515	24.103414149099095
32-33	24.82992288176562	25.470139941090274	25.281567576867175	24.41836960027693
34-35	24.79972514754636	25.72410854595994	25.427173441447497	24.048992865046202
36-37	24.904858409904172	25.691836221011847	25.33054988284078	24.072755486243196
38-39	24.977184459847116	25.81857642965924	25.1173342834078	24.086904827085842
40-41	24.845694309437597	25.504124073556056	25.262461095971318	24.387720521035032
42-43	24.619458601040034	25.537273076404098	25.232320978171742	24.610947344384126
44-45	24.466087590127156	25.52950109011496	25.55337411468705	24.45103720507084
46-47	24.46065717344059	25.38890006163028	25.46734568101463	24.683097083914497
48-49	24.42924684976438	25.65485458055677	25.381713063876603	24.53418550580225
50-51	24.470598797858106	25.599955161000032	25.497847831795628	24.431598209346234
52-53	24.570053451236223	25.5556798198554	25.348351610997273	24.525915117911108
54-55	24.24809913212167	25.309502445140673	25.688041256267248	24.75435716647041
56-57	24.415829616787757	25.460276881023574	25.341067173398745	24.782826328789923
58-59	24.53820017302735	25.62146348754426	25.424329596862883	24.416006742565507
60-61	24.892320874064662	25.515210787451693	25.472396025648376	24.12007231283527
62-63	24.72000903008341	25.49610318167723	25.46805283636736	24.315834951871988
64-65	24.53235955866689	25.26474044654865	25.492926718578136	24.709973276206327
66-67	24.4594551464603	25.472807273155652	25.44488709062616	24.622850489757887
68-69	24.336244607206375	25.371079731607153	25.51940263960803	24.77327302157844
70-71	24.348445010001768	25.325865070466463	25.64962184264492	24.67606807688685
72-73	24.32190974296273	25.771451732013645	25.29353854521129	24.613099979812333
74-75	24.20515889037773	25.660819356840896	25.504508216714083	24.62951353606729
76-77	24.256908062888087	25.102093242307006	25.549206892883188	25.091791801921726
78-79	24.158055859578326	25.300178158208432	25.68550431539348	24.85626166681976
80-81	24.408524502196357	25.504173846662027	25.359228974236085	24.728072676905526
82-83	24.394072241924274	25.515121304914288	25.506091258993397	24.584715194168044
84-85	24.39332430979255	25.630653120283657	25.23227486646336	24.743747703460432
86-87	24.071646894063498	25.726198467823274	25.45889920240971	24.743255435703514
88-89	24.068042546831407	25.379363458217085	25.60586575827023	24.94672823668128
90-91	24.409083089180314	25.159537381165965	25.611325344160395	24.82005418549333
92-93	24.411490636086004	25.380072071426845	25.560782598022612	24.64765469446453
94-95	24.37637836916304	25.226721373710053	25.48319956281419	24.91370069431272
96-97	24.05667293176989	25.28261082239276	25.652003660972365	25.008712584864988
98-99	23.89908187842557	25.274525668325143	25.540423350004595	25.285969103244692
100-101	23.960620385404145	25.387494359271646	25.636529759042613	25.015355496281593
102-103	23.814041302588976	25.31020016077629	25.771381093268413	25.104377443366317
104-105	24.000636250667192	25.301706589320172	25.604081345633833	25.0935758143788
106-107	23.99760335094924	25.119456192130503	25.787694675837386	25.09524578108287
108-109	23.95822332084067	25.12107104573259	25.81286852292935	25.10783711049739
110-111	23.71213045159315	25.6094807833531	25.674405569726677	25.003983195327077
112-113	23.747620857827524	25.654255988561953	25.668631391572745	24.929491762037777
114-115	23.827166964718483	25.733902866590764	25.585482402833897	24.85344776585686
116-117	23.66097967217981	25.515214403350804	25.72297899182668	25.100826932642704
118-119	23.683869232258676	25.287966347722012	25.833633305047194	25.19453111497212
120-121	23.571150164238	25.33196628359172	26.039988021447215	25.056895530723068
122-123	23.541280480590192	25.55784643615553	25.834110030078406	25.066763053175876
124-125	23.43744932133072	25.43345911806077	25.80912671425261	25.319964846355898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	253.0
1	253.5
2	227.0
3	174.5
4	144.5
5	136.5
6	136.5
7	182.0
8	239.0
9	281.0
10	291.0
11	289.5
12	313.0
13	339.5
14	380.0
15	419.5
16	435.5
17	482.0
18	528.0
19	536.5
20	614.0
21	704.5
22	776.0
23	963.0
24	1183.0
25	1436.0
26	1945.0
27	2721.0
28	3650.5
29	4916.0
30	6916.0
31	9381.5
32	12333.5
33	15579.0
34	19415.0
35	23594.0
36	27997.5
37	32601.0
38	36440.0
39	40887.0
40	45182.5
41	48800.0
42	52505.0
43	57219.5
44	62700.5
45	67951.0
46	72697.5
47	76833.5
48	82399.0
49	87147.5
50	88577.0
51	89178.0
52	88465.0
53	87212.5
54	86380.5
55	83987.5
56	79083.5
57	71541.0
58	64775.5
59	57818.5
60	48893.5
61	40982.5
62	33664.5
63	25645.0
64	18946.5
65	14273.0
66	10731.5
67	8056.0
68	5954.5
69	4567.5
70	3656.5
71	2840.5
72	2172.0
73	1690.0
74	1300.0
75	949.0
76	643.5
77	432.0
78	292.5
79	201.0
80	154.0
81	109.5
82	77.0
83	64.0
84	53.5
85	41.0
86	35.5
87	31.0
88	24.0
89	18.0
90	18.0
91	15.5
92	12.0
93	8.5
94	6.0
95	6.0
96	6.0
97	2.5
98	2.0
99	2.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01785150866005746
2	0.013077267971902555
3	0.012869692289808866
4	0.015827645759643967
5	0.009963632740497184
6	0.03471703283016988
7	0.015101130872316047
8	0.014582191667081818
9	0.009548481376309802
10-11	0.008847913449243594
12-13	0.007368936714326043
14-15	0.0065386339859512775
16-17	0.005033710290772015
18-19	0.006616474866736412
20-21	0.005812119098623359
22-23	0.005708331257576513
24-25	0.005941853899931915
26-27	0.004644505886846344
28-29	0.006305111343595875
30-31	0.005967800860193627
32-33	0.00838086816453279
34-35	0.008095451601653963
36-37	0.007420830634849466
38-39	0.007342989754064332
40-41	0.007835981999036849
42-43	0.00768030023746658
44-45	0.007628406316943157
46-47	0.010145261462329165
48-49	0.008718178647935037
50-51	0.005915906939670204
52-53	0.005786172138361647
54-55	0.006486740065427855
56-57	0.007861928959298561
58-59	0.007524618475896312
60-61	0.005319126853650841
62-63	0.005993747820455338
64-65	0.0038142031584715787
66-67	0.004488824125276076
68-69	0.006798103588568393
70-71	0.005111551171557149
72-73	0.004618558926584633
74-75	0.004255301482920673
76-77	0.004826134608678324
78-79	0.003580680516116176
80-81	0.0035547335558544645
82-83	0.005578596456267956
84-85	0.010171208422590877
86-87	0.007342989754064332
88-89	0.004981816370248592
90-91	0.005630490376791378
92-93	0.00879601952872017
94-95	0.007187307992494063
96-97	0.01024904930337601
98-99	0.007213254952755774
100-101	0.009003595210813863
102-103	0.0055526494960062445
104-105	0.004903975489463458
106-107	0.007835981999036849
108-109	0.007446777595111177
110-111	0.008458709045317923
112-113	0.00547480861522111
114-115	0.002594696026171142
116-117	0.003502839635331042
118-119	0.16686490144306615
120-121	0.011987495640910676
122-123	0.002283332503030605
124-125	0.001997915940151779
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1927008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.72232188493463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.2436891343061	50.145491318672065
2	10.041154426389243	12.395267312038659
3	3.3394553797549276	6.183568196088307
4	1.5702851947739356	3.876865929719364
5	0.9237506124573837	2.850801632175007
6	0.5816705900584268	2.154123563435164
7	0.40359984752006955	1.7437783790940988
8	0.3143243985303612	1.552066536190354
9	0.24402211866816917	1.3555450579932409
>10	1.261327179335412	13.647953229096283
>50	0.06158791860593798	2.5308998680601587
>100	0.014797124167094909	1.3968623947662293
>500	2.520565747545251E-4	0.10236846212460073
>1k	8.401885825150821E-5	0.06440812054648123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0378784104684568E-4
2	0.0	0.0	0.0	0.0	1.0378784104684568E-4
3	0.0	0.0	0.0	0.0	1.0378784104684568E-4
4	0.0	0.0	0.0	0.0	1.556817615702685E-4
5	0.0	0.0	0.0	0.0	1.556817615702685E-4
6	0.0	0.0	0.0	0.0	1.556817615702685E-4
7	0.0	0.0	0.0	0.0	2.0757568209369136E-4
8	0.0	0.0	0.0	0.0	2.0757568209369136E-4
9	0.0	0.0	0.0	5.189392052342284E-5	2.0757568209369136E-4
10-11	0.0	0.0	0.0	5.189392052342284E-5	2.594696026171142E-4
12-13	0.0	0.0	0.0	1.0378784104684568E-4	3.11363523140537E-4
14-15	0.0	0.0	0.0	1.0378784104684568E-4	3.6325744366395987E-4
16-17	0.0	0.0	0.0	1.8162872183197993E-4	3.892044039256713E-4
18-19	0.0	0.0	0.0	2.0757568209369136E-4	4.151513641873827E-4
20-21	0.0	0.0	0.0	2.0757568209369136E-4	4.92992244972517E-4
22-23	0.0	0.0	0.0	2.594696026171142E-4	6.22727046281074E-4
24-25	0.0	0.0	0.0	4.670452847108056E-4	7.784088078513426E-4
26-27	0.0	0.0	0.0	5.967800860193626E-4	8.303027283747654E-4
28-29	0.0	0.0	0.0	0.0015049236951792625	8.303027283747654E-4
30-31	0.0	0.0	0.0	0.0066164748667364125	8.562496886364768E-4
32-33	0.0	0.0	0.0	0.0195380610770687	9.85984489945034E-4
34-35	0.0	0.0	0.0	0.036092221724040585	0.0010897723309918797
36-37	0.0	0.0	0.0	0.05972990252245969	0.0010897723309918797
38-39	0.0	0.0	0.0	0.1049554542586227	0.0011935601720387253
40-41	0.0	0.0	0.0	0.16349179660904364	0.0011935601720387253
42-43	0.0	0.0	0.0	0.21782473139706737	0.0011935601720387253
44-45	0.0	0.0	0.0	0.276931906873246	0.0012195071323004365
46-47	0.0	5.189392052342284E-5	0.0	0.3394640811039705	0.001245454092562148
48-49	0.0	5.189392052342284E-5	0.0	0.40887219980404854	0.001297348013085571
50-51	0.0	5.189392052342284E-5	0.0	0.4870244441123234	0.0013492419336089939
52-53	0.0	5.189392052342284E-5	0.0	0.5682903236520036	0.0014530297746558397
54-55	0.0	5.189392052342284E-5	0.0	0.6548493830850728	0.0015568176157026853
56-57	0.0	5.189392052342284E-5	0.0	0.7393326856972052	0.0015827645759643968
58-59	0.0	5.189392052342284E-5	0.0	0.8367635214799316	0.001608711536226108
60-61	0.0	5.189392052342284E-5	0.0	0.9445990883276043	0.001608711536226108
62-63	0.0	5.189392052342284E-5	0.0	1.0618015078297547	0.0016606054567495309
64-65	0.0	5.189392052342284E-5	0.0	1.1731139673524966	0.0016606054567495309
66-67	0.0	5.189392052342284E-5	0.0	1.295376044105681	0.0016606054567495309
68-69	0.0	5.189392052342284E-5	0.0	1.4372799697769807	0.0016606054567495309
70-71	0.0	5.189392052342284E-5	0.0	1.5905227170826484	0.001738446337534665
72-73	0.0	5.189392052342284E-5	0.0	1.737200883442103	0.0020757568209369137
74-75	0.0	5.189392052342284E-5	0.0	1.891585296999286	0.0020757568209369137
76-77	0.0	5.189392052342284E-5	0.0	2.063328226971554	0.0020757568209369137
78-79	0.0	5.189392052342284E-5	0.0	2.258267739417792	0.0020757568209369137
80-81	0.0	5.189392052342284E-5	0.0	2.4585523256779425	0.002101703781198625
82-83	0.0	5.189392052342284E-5	0.0	2.6751056560221858	0.002205491622245471
84-85	0.0	5.189392052342284E-5	0.0	2.891477357644597	0.0022314385825071823
86-87	0.0	5.189392052342284E-5	0.0	3.1220160995699033	0.0022314385825071823
88-89	0.0	5.189392052342284E-5	0.0	3.3865972533585746	0.0022314385825071823
90-91	0.0	5.189392052342284E-5	0.0	3.6687444992444247	0.0022314385825071823
92-93	0.0	5.189392052342284E-5	0.0	3.953538335076969	0.0022314385825071823
94-95	0.0	5.189392052342284E-5	0.0	4.241316071339611	0.002413067304339162
96-97	0.0	5.189392052342284E-5	0.0	4.5542623590561115	0.002542802105647719
98-99	0.0	5.189392052342284E-5	0.0	4.902626247529849	0.002594696026171142
100-101	0.0	5.189392052342284E-5	0.0	5.277585770271841	0.002594696026171142
102-103	2.594696026171142E-5	5.189392052342284E-5	0.0	5.679374449924443	0.0026725369069562762
104-105	5.189392052342284E-5	5.189392052342284E-5	0.0	6.093306306979525	0.0026984838672179877
106-107	5.189392052342284E-5	5.189392052342284E-5	0.0	6.533392699978412	0.0026984838672179877
108-109	5.189392052342284E-5	5.189392052342284E-5	0.0	6.985726058220827	0.0027244308274796993
110-111	5.189392052342284E-5	5.189392052342284E-5	0.0	7.46182683206297	0.002828218668526545
112-113	5.189392052342284E-5	5.189392052342284E-5	0.0	7.959723052524951	0.0028541656287882564
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1840	0.0	60.80369	1
GTATCAA	3450	0.0	54.507706	1
TCAACGC	4245	0.0	41.916344	4
CAACGCA	4375	0.0	40.669773	5
TATCAAC	4785	0.0	38.056545	2
ATCAACG	4740	0.0	37.7901	3
GTACATG	10580	0.0	37.74216	1
AACGCAG	4795	0.0	37.72701	6
TACATGG	11050	0.0	36.136837	2
ACATGGG	11475	0.0	34.27983	3
CATGGGG	10375	0.0	31.031237	4
ACGCAGA	6030	0.0	29.69794	7
ATGGGGG	6540	0.0	28.844309	5
CGCAGAG	6240	0.0	28.602407	8
GCAGAGT	7275	0.0	24.286604	9
TGGGGGG	4705	0.0	22.892136	6
AGAGTAC	6535	0.0	22.074875	10-11
GTATAGG	510	0.0	21.003527	1
AGTACAT	7405	0.0	20.814434	2
CAGAGTA	6940	0.0	20.743784	10-11
>>END_MODULE