FastQCFastQC Report
Mon 15 Aug 2016
SRR2921342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927008
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.063.097911
GTATCAA39350.051.211851
GTACATG102350.040.4884071
TACATGG105650.038.925012
ACATGGG106100.037.9173133
TCAACGC52550.036.860384
CATGGGG94900.036.739974
TATCAAC56700.035.108522
CAACGCA55400.034.426225
AACGCAG57750.033.531766
ATGGGGG56750.032.9771355
ATCAACG59450.032.3817143
TGGGGGG38350.027.3446436
ACGCAGA72600.026.4219727
CGCAGAG77350.024.7179078
GTAAGTA9400.023.459732
AGAGTAC77650.022.3572810-11
ATGGGGA33950.022.2952885
CAGAGTA81500.020.973949
GCAGAGT86650.020.8271479
Produced by FastQC (version 0.11.5)