FastQCFastQC Report
Mon 15 Aug 2016
SRR2921341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372567
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29550.054.980691
GGTATCA16600.051.624921
TCAACGC37650.042.0426454
CAACGCA38850.040.741065
GTACATG84050.040.2883571
ATCAACG40350.039.5243383
AACGCAG40450.038.9796036
TACATGG86600.038.6181762
TATCAAC41800.038.1532822
ACATGGG88000.037.7333073
CATGGGG73350.036.5889364
ATGGGGG48150.035.4672365
ACGCAGA50600.030.9242697
TGGGGGG40150.029.3426
CGCAGAG53000.029.1860898
GCAGAGT60250.025.4737919
GAGTACA58100.023.5589921
AGAGTAC54000.022.69365110-11
ATGGGGA21900.022.5515145
AGTACAT56600.022.394252
Produced by FastQC (version 0.11.5)