##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921341_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1372567 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.058698773903206 31.0 31.0 34.0 26.0 34.0 2 30.03457026141529 31.0 31.0 34.0 26.0 34.0 3 30.21378264230453 33.0 31.0 34.0 26.0 34.0 4 33.540103324646445 37.0 35.0 37.0 28.0 37.0 5 33.64544973032282 37.0 35.0 37.0 28.0 37.0 6 33.593565924286395 37.0 35.0 37.0 28.0 37.0 7 33.5395445176811 37.0 35.0 37.0 28.0 37.0 8 33.58749773235113 37.0 35.0 37.0 28.0 37.0 9 35.128333990253296 39.0 37.0 39.0 28.0 39.0 10-11 35.14061207941033 39.0 37.0 39.0 27.5 39.0 12-13 35.047505877673004 39.0 37.0 39.0 27.0 39.0 14-15 36.30366532198428 40.0 38.0 41.0 27.0 41.0 16-17 36.28938842329737 40.0 38.0 41.0 27.0 41.0 18-19 36.27716643340544 40.0 38.0 41.0 27.0 41.0 20-21 36.32219556495238 40.0 38.0 41.0 27.0 41.0 22-23 36.28015171572681 40.0 38.0 41.0 27.0 41.0 24-25 36.27875069122309 40.0 37.5 41.0 27.0 41.0 26-27 36.26065867822846 40.0 37.0 41.0 27.0 41.0 28-29 36.19137572154948 40.0 37.0 41.0 27.0 41.0 30-31 36.100494183526195 40.0 37.0 41.0 27.0 41.0 32-33 36.002852319777475 40.0 37.0 41.0 27.0 41.0 34-35 35.87858188343447 40.0 36.5 41.0 26.5 41.0 36-37 35.752645590342766 40.0 36.0 41.0 26.0 41.0 38-39 35.63929775377085 40.0 36.0 41.0 25.0 41.0 40-41 35.524167490548734 40.0 35.5 41.0 25.0 41.0 42-43 35.29639136013033 40.0 35.0 41.0 24.0 41.0 44-45 34.99341234344116 40.0 35.0 41.0 23.0 41.0 46-47 34.72995744470033 39.5 35.0 41.0 22.0 41.0 48-49 34.49653277399209 39.0 35.0 41.0 19.5 41.0 50-51 34.307886245261614 39.0 34.5 41.0 18.5 41.0 52-53 34.12246870280285 39.0 34.0 41.0 17.5 41.0 54-55 33.923020879855045 38.5 34.0 40.0 15.5 41.0 56-57 33.683906869391436 38.0 34.0 40.0 13.5 41.0 58-59 33.417908561112135 38.0 33.5 40.0 10.5 41.0 60-61 33.13230538108522 37.0 33.0 40.0 8.0 41.0 62-63 32.7998134153014 37.0 33.0 40.0 7.0 41.0 64-65 32.465446859789 36.0 32.5 39.0 4.0 41.0 66-67 32.13490161136032 36.0 32.0 39.0 2.0 40.5 68-69 31.787176145135355 35.0 32.0 38.5 2.0 40.0 70-71 31.758546941606497 35.0 32.0 38.5 2.0 40.0 72-73 31.477128985324576 35.0 32.0 37.0 2.0 39.5 74-75 31.15034238765758 35.0 32.0 37.0 2.0 39.0 76-77 30.84621661456235 35.0 32.0 36.5 2.0 39.0 78-79 30.55430299577361 35.0 32.0 36.0 2.0 37.5 80-81 30.272191084296793 35.0 31.0 35.5 2.0 37.0 82-83 30.04232725979861 35.0 31.0 35.0 2.0 37.0 84-85 29.818948728914506 35.0 31.0 35.0 2.0 36.0 86-87 29.642086324383435 35.0 31.0 35.0 2.0 36.0 88-89 29.49260363974946 35.0 31.0 35.0 2.0 36.0 90-91 29.361382723029188 35.0 31.0 35.0 2.0 35.0 92-93 29.239885921780136 35.0 31.0 35.0 2.0 35.0 94-95 29.108805981784496 34.0 30.0 35.0 2.0 35.0 96-97 29.013441602486438 34.0 30.0 35.0 2.0 35.0 98-99 28.926413428269804 34.0 30.0 35.0 2.0 35.0 100-101 28.827551223364686 34.0 30.0 35.0 2.0 35.0 102-103 27.96467349134869 33.5 28.0 34.5 2.0 35.0 104-105 28.558870714507925 34.0 29.5 35.0 2.0 35.0 106-107 28.636637045768985 34.0 30.0 35.0 2.0 35.0 108-109 28.57401350899446 34.0 30.0 35.0 2.0 35.0 110-111 28.491177115579788 34.0 29.0 35.0 2.0 35.0 112-113 28.397794424607326 34.0 29.0 35.0 2.0 35.0 114-115 28.259865274336335 34.0 29.0 35.0 2.0 35.0 116-117 28.118108988486537 34.0 29.0 35.0 2.0 35.0 118-119 28.115044657200706 34.0 29.0 35.0 2.0 35.0 120-121 28.286265442779843 34.0 30.0 35.0 2.0 35.0 122-123 28.149216030984277 34.0 30.0 35.0 2.0 35.0 124-125 27.548064684638344 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61878.0 3 8611.0 4 5462.0 5 4480.0 6 4816.0 7 5490.0 8 5440.0 9 5814.0 10 6568.0 11 7016.0 12 7047.0 13 7252.0 14 7968.0 15 7149.0 16 5403.0 17 4816.0 18 4529.0 19 4190.0 20 4058.0 21 4313.0 22 4722.0 23 6050.0 24 8784.0 25 11002.0 26 11696.0 27 12631.0 28 15208.0 29 18023.0 30 22018.0 31 27784.0 32 36132.0 33 49930.0 34 80413.0 35 128101.0 36 250898.0 37 408439.0 38 108379.0 39 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51162326357894 17.99968082571408 12.48007893369555 25.00861697701143 2 16.126387979093696 19.613467996785214 39.62820121406957 24.63194281005152 3 19.87714996666436 24.639155351372228 28.41508155391448 27.068613128048934 4 13.350316095052944 15.33243173133815 34.82692739286433 36.49032478074458 5 14.187504963812442 38.39954300137055 32.38096140212309 15.031990632693908 6 33.14420855470771 34.15987056431335 17.465655085962496 15.230265795016434 7 29.43785969008177 32.65749124502136 19.796234521212334 18.108414543684535 8 27.77669287437061 35.225194371780205 18.989266742933758 18.008846010915423 9 28.41656350482608 16.722710444690073 18.202703362408094 36.658022688075754 10-11 26.528732684175093 26.06951540463582 26.15348968701091 21.248262224178184 12-13 26.120137038003126 23.60470131864833 26.466484415643084 23.80867722770546 14-15 24.00529991824982 24.850998998888137 24.059763609365277 27.083937473496768 16-17 24.228904294733315 27.599991256798752 24.580745285046575 23.59035916342136 18-19 24.17241933303922 26.914427501467237 26.068440366504564 22.844712798988983 20-21 25.15572141877167 25.814964556938047 25.875621045943735 23.153692978346545 22-23 24.951447503740482 25.308719558377202 25.78358865516416 23.956244282718153 24-25 24.44118595319066 25.38465882078642 25.873918246680738 24.300236979342177 26-27 23.92107389513581 25.718670833275652 26.063661977167197 24.296593294421342 28-29 24.028799046394983 26.32978645892533 25.780596527735277 23.860817966944413 30-31 25.046676335271062 25.706647839482223 25.798197209656802 23.448478615589917 32-33 24.512321609621942 25.712841994205238 25.80898417533944 23.96585222083338 34-35 24.464169915115306 26.135086888411234 25.931618638201755 23.4691245582717 36-37 24.417733273732303 26.069257417067682 25.92473584830736 23.588273460892655 38-39 24.520751060140185 26.236393046063274 25.70574005799805 23.537115835798495 40-41 24.60293866916146 25.782209728822302 25.852812982255198 23.762038619761043 42-43 24.285671089019385 25.885062546472227 25.591647695250753 24.237618669257635 44-45 23.836879941623135 26.000037159512306 26.072898948349373 24.090183950515186 46-47 23.88115926189045 25.804069439131467 25.849317813359562 24.46545348561852 48-49 23.8725603776032 26.156074628801424 25.85981524956173 24.111549744033645 50-51 23.914190245672426 26.144572080572516 25.91152645069721 24.029711223057856 52-53 24.11014607429654 26.06440937670425 25.710088951694605 24.1153555973046 54-55 23.646443619820225 25.726988503268178 26.258727832961863 24.367840043949734 56-57 23.8350533040962 25.96776361783679 25.624328074753606 24.572855003313403 58-59 23.968104023318688 26.156580712828735 25.813219532934074 24.0620957309185 60-61 24.446197142050472 26.11218681408756 25.889671126330843 23.551944917531127 62-63 24.2236328053847 26.055715120489744 25.84598559398229 23.874666480143272 64-65 24.02673786466479 25.697726902635722 25.80963861478655 24.46589661791294 66-67 24.045490345796864 25.960739112473412 25.681506377726667 24.312264164003057 68-69 23.770484039371322 25.987179328379252 25.894135468071823 24.348201164177606 70-71 23.850542260627545 25.83943839503969 26.040714173822128 24.269305170510638 72-73 23.887704107616837 26.477687779736087 25.43320614441249 24.201401968234585 74-75 23.84633347285929 26.25015072810326 25.648659075434725 24.254856723602728 76-77 23.650697834202127 25.723490005256842 25.820393713208013 24.805418447333018 78-79 23.687750884413663 25.735153489622554 26.032672031253473 24.544423594710313 80-81 24.023332196741187 26.06562799630462 25.600135225322106 24.310904581632087 82-83 24.047059063540082 26.13851632946882 25.8055079534393 24.008916653551797 84-85 24.030475960088193 26.32273069731993 25.34584742638219 24.30094591620969 86-87 23.554679758449037 26.535231525198476 25.594733268049293 24.315355448303198 88-89 23.517709165530963 26.056916033604484 25.843363420863675 24.582011380000882 90-91 24.067258924856848 25.7090231091442 25.93430757449797 24.289410391500976 92-93 24.220377742326004 25.907988197692656 25.738072277656602 24.133561782324737 94-95 24.01759754077428 25.959614984577012 25.58281106724436 24.439976407404345 96-97 23.560155825627728 25.847373803355577 25.81334660418389 24.779123766832807 98-99 23.389948075121854 25.86054201077114 25.668114306864993 25.081395607242012 100-101 23.411318357119384 26.22083394355904 25.745331500106015 24.62251619921556 102-103 23.33292410010889 26.176560699548375 25.82446226207854 24.666052938264194 104-105 23.4968712101424 26.100668453922072 25.614036621613696 24.788423714321834 106-107 23.567308494488547 26.033936860113645 25.812509722502032 24.586244922895776 108-109 23.506104691617807 26.049331533171237 25.757703816989675 24.686859958221277 110-111 23.22372658570209 26.67379259893971 25.681736046142234 24.420744769215972 112-113 23.201724458893462 26.6890760833333 25.724587127688135 24.384612330085098 114-115 23.32889385618134 26.849243807439375 25.522865007351363 24.298997329027916 116-117 23.229455254831887 26.674535390130217 25.527559657491956 24.56844969754594 118-119 23.085764409857028 26.365418970278302 25.86111776004297 24.687698859821705 120-121 23.045826570557836 26.428799131450138 26.01303189115438 24.512342406837647 122-123 23.095381295315214 26.571786510906612 25.765834619575333 24.566997574202837 124-125 22.932903961429385 26.548844393363435 25.68100557539903 24.83724606980815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 847.0 1 730.5 2 554.0 3 392.5 4 272.0 5 247.0 6 248.5 7 313.5 8 409.5 9 457.0 10 448.5 11 442.0 12 468.5 13 506.0 14 585.0 15 633.5 16 652.5 17 712.0 18 779.0 19 872.0 20 961.0 21 1039.5 22 1151.5 23 1336.5 24 1585.5 25 1947.0 26 2581.5 27 3395.0 28 4082.0 29 5094.5 30 6808.5 31 8870.5 32 11541.0 33 14382.5 34 17151.5 35 20102.5 36 23329.0 37 26427.0 38 28588.0 39 31028.5 40 33360.0 41 35143.5 42 37348.5 43 40279.5 44 43369.5 45 46386.5 46 49819.5 47 53072.0 48 56206.0 49 59430.5 50 61819.5 51 62485.5 52 61412.0 53 60221.5 54 58670.0 55 55668.0 56 52026.0 57 47008.0 58 42071.5 59 37140.0 60 31535.0 61 26810.0 62 22208.0 63 17350.0 64 13283.5 65 10367.5 66 7962.5 67 6043.0 68 4835.0 69 3966.0 70 3224.0 71 2467.5 72 1853.5 73 1588.0 74 1264.0 75 974.0 76 719.0 77 484.0 78 340.5 79 223.0 80 146.5 81 100.5 82 66.5 83 42.5 84 39.0 85 33.0 86 26.5 87 26.0 88 18.5 89 12.5 90 11.5 91 10.5 92 10.0 93 7.0 94 5.5 95 4.0 96 4.5 97 4.5 98 3.5 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0201083080097365 2 0.011511277773689736 3 0.011802702527454034 4 0.014206956746009485 5 0.009762729251103953 6 0.03329527811757095 7 0.014935518630420228 8 0.014352669122891633 9 0.009325592120457507 10-11 0.008961311178252137 12-13 0.007358475032548502 14-15 0.006775625525019907 16-17 0.005245645567757348 18-19 0.006374916488594 20-21 0.00611991982905024 22-23 0.006083491734829702 24-25 0.006156347923270776 26-27 0.004954220813993051 28-29 0.006629913148137759 30-31 0.006083491734829702 32-33 0.008779170707149451 34-35 0.008524174047605691 36-37 0.007832040257415485 38-39 0.0077956121631949475 40-41 0.007941324540077095 42-43 0.007832040257415485 44-45 0.007686327880533337 46-47 0.010345578758632549 48-49 0.008815598801369989 50-51 0.006484200771255611 52-53 0.005864923169506479 54-55 0.006557056959696685 56-57 0.008123465011179783 58-59 0.007686327880533337 60-61 0.005792066981065405 62-63 0.006192776017491314 64-65 0.004116374646920697 66-67 0.00407994655270016 68-69 0.006994194090343131 70-71 0.005245645567757348 72-73 0.004954220813993051 74-75 0.004553511777567142 76-77 0.005136361285095737 78-79 0.0034606689509510285 80-81 0.003132816102966194 82-83 0.005755638886844868 84-85 0.010272722570191474 86-87 0.0075041874094306515 88-89 0.005209217473536811 90-91 0.005828495075285942 92-93 0.008997739272472672 94-95 0.007467759315210114 96-97 0.009872013533765565 98-99 0.007540615503651188 100-101 0.00903416736669321 102-103 0.005937779357947553 104-105 0.005209217473536811 106-107 0.007759184068974411 108-109 0.00713990646722528 110-111 0.008378461670723541 112-113 0.005719210792624331 114-115 0.0024042542185554513 116-117 0.0032421003856278057 118-119 0.1642907049346225 120-121 0.011984842998556719 122-123 0.002076401370570617 124-125 0.0014206956746009485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1372567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.60766549872658 #Duplication Level Percentage of deduplicated Percentage of total 1 84.02333677564532 56.8062164681468 2 9.223867799022678 12.47208337521601 3 2.7753238987255453 5.628995093869752 4 1.2387459691093592 3.349948924697658 5 0.6939915947604294 2.345957579874546 6 0.4310368868464499 1.748483859811635 7 0.307625845648504 1.4558505689956864 8 0.21731199563355588 1.1753565367723342 9 0.16809192741113774 1.0227872521303587 >10 0.8648189564266255 10.242695714180048 >50 0.04042629121018023 1.8471782042350262 >100 0.01477500514277518 1.4966622964423235 >500 4.313696116441417E-4 0.2453412449020923 >1k 2.1568480582207086E-4 0.1624428807256647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.285618844107428E-5 0.0 10-11 0.0 0.0 0.0 7.285618844107428E-5 0.0 12-13 0.0 0.0 0.0 7.285618844107428E-5 0.0 14-15 0.0 0.0 0.0 7.285618844107428E-5 7.285618844107428E-5 16-17 0.0 0.0 0.0 7.285618844107428E-5 1.4571237688214855E-4 18-19 0.0 0.0 0.0 7.285618844107428E-5 1.4571237688214855E-4 20-21 0.0 0.0 0.0 1.0928428266161142E-4 1.4571237688214855E-4 22-23 0.0 0.0 0.0 4.371371306464457E-4 1.4571237688214855E-4 24-25 0.0 0.0 0.0 0.00101998663817504 1.4571237688214855E-4 26-27 0.0 7.285618844107428E-5 0.0 0.0017121204283652458 1.4571237688214855E-4 28-29 0.0 7.285618844107428E-5 0.0 0.004116374646920697 1.4571237688214855E-4 30-31 0.0 7.285618844107428E-5 0.0 0.013806247709583577 1.8214047110268569E-4 32-33 0.0 7.285618844107428E-5 0.0 0.034606689509510284 2.1856856532322284E-4 34-35 0.0 7.285618844107428E-5 0.0 0.061308482573164 2.914247537642971E-4 36-37 0.0 7.285618844107428E-5 0.0 0.09864727914921458 3.6428094220537137E-4 38-39 0.0 7.285618844107428E-5 0.0 0.1604657550414661 3.6428094220537137E-4 40-41 0.0 7.285618844107428E-5 0.0 0.24315752892208542 3.6428094220537137E-4 42-43 0.0 7.285618844107428E-5 0.0 0.3186729682412589 3.6428094220537137E-4 44-45 0.0 7.285618844107428E-5 0.0 0.39542696276393063 3.6428094220537137E-4 46-47 0.0 7.285618844107428E-5 0.0 0.4743302148456141 3.6428094220537137E-4 48-49 0.0 7.285618844107428E-5 0.0 0.5631419085552837 3.6428094220537137E-4 50-51 0.0 7.285618844107428E-5 0.0 0.6616434753276161 3.6428094220537137E-4 52-53 0.0 7.285618844107428E-5 0.0 0.7708549018007864 3.6428094220537137E-4 54-55 0.0 7.285618844107428E-5 0.0 0.8821427296445274 3.6428094220537137E-4 56-57 0.0 2.1856856532322284E-4 0.0 0.9954341026703979 4.0070903642590853E-4 58-59 0.0 2.1856856532322284E-4 0.0 1.1225681515000725 4.371371306464457E-4 60-61 0.0 2.1856856532322284E-4 0.0 1.2537821468824473 4.371371306464457E-4 62-63 0.0 2.1856856532322284E-4 0.0 1.4031373331866495 4.371371306464457E-4 64-65 0.0 2.1856856532322284E-4 0.0 1.5443326263854515 4.371371306464457E-4 66-67 0.0 2.1856856532322284E-4 0.0 1.6954363612122396 4.371371306464457E-4 68-69 0.0 2.1856856532322284E-4 0.0 1.8639891531706647 4.371371306464457E-4 70-71 0.0 2.1856856532322284E-4 0.0 2.0437982262432364 4.371371306464457E-4 72-73 0.0 2.1856856532322284E-4 0.0 2.2159938276237154 5.0999331908752E-4 74-75 0.0 2.1856856532322284E-4 0.0 2.3925972284048793 5.0999331908752E-4 76-77 0.0 2.1856856532322284E-4 0.0 2.6089436799806496 5.0999331908752E-4 78-79 0.0 2.1856856532322284E-4 0.0 2.8514090751125445 5.0999331908752E-4 80-81 0.0 2.914247537642971E-4 0.0 3.078611098766035 5.0999331908752E-4 82-83 0.0 2.914247537642971E-4 0.0 3.3195100858464466 5.0999331908752E-4 84-85 0.0 2.914247537642971E-4 0.0 3.575672444405264 5.0999331908752E-4 86-87 0.0 3.6428094220537137E-4 0.0 3.851287405277848 5.0999331908752E-4 88-89 0.0 4.371371306464457E-4 0.0 4.162638326580779 5.0999331908752E-4 90-91 0.0 4.371371306464457E-4 0.0 4.481311294822038 5.0999331908752E-4 92-93 0.0 4.371371306464457E-4 0.0 4.789602256210443 5.0999331908752E-4 94-95 0.0 4.371371306464457E-4 0.0 5.112683023852387 5.0999331908752E-4 96-97 0.0 4.371371306464457E-4 0.0 5.476854681775097 5.0999331908752E-4 98-99 0.0 4.371371306464457E-4 0.0 5.883027932334086 5.0999331908752E-4 100-101 0.0 4.371371306464457E-4 0.0 6.310001624693002 5.0999331908752E-4 102-103 0.0 4.371371306464457E-4 0.0 6.754715798937319 5.0999331908752E-4 104-105 0.0 4.371371306464457E-4 0.0 7.221687538750386 5.0999331908752E-4 106-107 0.0 4.371371306464457E-4 0.0 7.732518704004978 5.0999331908752E-4 108-109 0.0 4.371371306464457E-4 0.0 8.252748317568468 5.0999331908752E-4 110-111 0.0 4.371371306464457E-4 0.0 8.813194547151433 6.557056959696685E-4 112-113 0.0 4.371371306464457E-4 0.0 9.369378689710594 7.285618844107427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2955 0.0 54.98069 1 GGTATCA 1660 0.0 51.62492 1 TCAACGC 3765 0.0 42.042645 4 CAACGCA 3885 0.0 40.74106 5 GTACATG 8405 0.0 40.288357 1 ATCAACG 4035 0.0 39.524338 3 AACGCAG 4045 0.0 38.979603 6 TACATGG 8660 0.0 38.618176 2 TATCAAC 4180 0.0 38.153282 2 ACATGGG 8800 0.0 37.733307 3 CATGGGG 7335 0.0 36.588936 4 ATGGGGG 4815 0.0 35.467236 5 ACGCAGA 5060 0.0 30.924269 7 TGGGGGG 4015 0.0 29.342 6 CGCAGAG 5300 0.0 29.186089 8 GCAGAGT 6025 0.0 25.473791 9 GAGTACA 5810 0.0 23.558992 1 AGAGTAC 5400 0.0 22.693651 10-11 ATGGGGA 2190 0.0 22.551514 5 AGTACAT 5660 0.0 22.39425 2 >>END_MODULE